1. Comparative genomics of Cluster O mycobacteriophages
- Author
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Cresawn, SG, Pope, WH, Jacobs-Sera, D, Bowman, CA, Russell, DA, Dedrick, RM, Adair, T, Anders, KR, Ball, S, Bollivar, D, Breitenberger, C, Burnett, SH, Butela, K, Byrnes, D, Carzo, S, Cornely, KA, Cross, T, Daniels, RL, Dunbar, D, Findley, AM, Gissendanner, CR, Golebiewska, UP, Hartzog, GA, Hatherill, JR, Hughes, LE, Jalloh, CS, De Los Santos, C, Ekanem, K, Khambule, SL, King, RA, King-Smith, C, Klyczek, K, Krukonis, GP, Laing, C, Lapin, JS, Lopez, AJ, Mkhwanazi, SM, Molloy, SD, Moran, D, Munsamy, V, Pacey, E, Plymale, R, Poxleitner, M, Reyna, N, Schildbach, JF, Stukey, J, Taylor, SE, Ware, VC, Wellmann, AL, Westholm, D, Wodarski, D, Zajko, M, Zikalala, TS, Hendrix, RW, Hatfull, GF, Cresawn, SG, Pope, WH, Jacobs-Sera, D, Bowman, CA, Russell, DA, Dedrick, RM, Adair, T, Anders, KR, Ball, S, Bollivar, D, Breitenberger, C, Burnett, SH, Butela, K, Byrnes, D, Carzo, S, Cornely, KA, Cross, T, Daniels, RL, Dunbar, D, Findley, AM, Gissendanner, CR, Golebiewska, UP, Hartzog, GA, Hatherill, JR, Hughes, LE, Jalloh, CS, De Los Santos, C, Ekanem, K, Khambule, SL, King, RA, King-Smith, C, Klyczek, K, Krukonis, GP, Laing, C, Lapin, JS, Lopez, AJ, Mkhwanazi, SM, Molloy, SD, Moran, D, Munsamy, V, Pacey, E, Plymale, R, Poxleitner, M, Reyna, N, Schildbach, JF, Stukey, J, Taylor, SE, Ware, VC, Wellmann, AL, Westholm, D, Wodarski, D, Zajko, M, Zikalala, TS, Hendrix, RW, and Hatfull, GF
- Abstract
Mycobacteriophages - viruses of mycobacterial hosts - are genetically diverse but morphologically are all classified in the Caudovirales with double-stranded DNA and tails. We describe here a group of five closely related mycobacteriophages - Corndog, Catdawg, Dylan, Firecracker, and YungJamal - designated as Cluster O with long flexible tails but with unusual prolate capsids. Proteomic analysis of phage Corndog particles, Catdawg particles, and Corndog-infected cells confirms expression of half of the predicted gene products and indicates a non-canonical mechanism for translation of the Corndog tape measure protein. Bioinformatic analysis identifies 8-9 strongly predicted SigA promoters and all five Cluster O genomes contain more than 30 copies of a 17 bp repeat sequence with dyad symmetry located throughout the genomes. Comparison of the Cluster O phages provides insights into phage genome evolution including the processes of gene flux by horizontal genetic exchange.
- Published
- 2015