Search

Your search keyword '"Dabrowska, Agnieszka"' showing total 3 results
Start Over Author "Dabrowska, Agnieszka" Publication Type Electronic Resources
3 results on '"Dabrowska, Agnieszka"'

1. L-Arginine Improves Solubility and ANTI SARS-CoV-2 Mpro Activity of Rutin but Not the Antiviral Activity in Cells

Author

Fundación hna, Instituto de Investigación Sanitaria Aragón, Instituto de Salud Carlos III, European Commission, Ministerio de Economía y Competitividad (España), Diputación General de Aragón, Centro de Investigación Biomédica en Red Enfermedades Hepáticas y Digestivas (España), Ministry of Science and Higher Education (Poland), Sancineto, Luca [0000-0002-6199-7399], Ostacolo, Carmine[0000-0003-3715-8680], Ortega-Alarcon, David [0000-0003-1885-4365], Ceballos-Laita, Laura [0000-0002-7223-1719], Abian, Olga [0000-0001-5664-1729], Velázquez-Campoy, Adrián [0000-0001-5702-4538], Moretti, Silvia [0000-0002-8177-7254], Dabrowska, Agnieszka [0000-0003-1499-4950], Botwina, Pawel [0000-0001-9006-1568], Synowiec, Aleksandra [0000-0001-9846-6922], Kula-Pacurar, Anna [0000-0001-8404-2176], Pyrc, Krzysztof [0000-0002-3867-7688], Iraci, Nunzio [0000-0002-1359-8684], Santi, Claudio [0000-0002-7698-8970], Sancineto, Luca, Ostacolo, Carmine, Ortega-Alarcón, David, Jiménez-Alesanco, Ana, Ceballos-Laita, Laura, Vega, Sonia, Abian, Olga, Velázquez-Campoy, Adrián, Moretti, Silvia, Dabrowska, Agnieszka, Botwina, Pawel, Synowiec, Aleksandra, Kula-Pacurar, Anna, Pyrc, Krzysztof, Iraci, Nunzio, Santi, Claudio, Fundación hna, Instituto de Investigación Sanitaria Aragón, Instituto de Salud Carlos III, European Commission, Ministerio de Economía y Competitividad (España), Diputación General de Aragón, Centro de Investigación Biomédica en Red Enfermedades Hepáticas y Digestivas (España), Ministry of Science and Higher Education (Poland), Sancineto, Luca [0000-0002-6199-7399], Ostacolo, Carmine[0000-0003-3715-8680], Ortega-Alarcon, David [0000-0003-1885-4365], Ceballos-Laita, Laura [0000-0002-7223-1719], Abian, Olga [0000-0001-5664-1729], Velázquez-Campoy, Adrián [0000-0001-5702-4538], Moretti, Silvia [0000-0002-8177-7254], Dabrowska, Agnieszka [0000-0003-1499-4950], Botwina, Pawel [0000-0001-9006-1568], Synowiec, Aleksandra [0000-0001-9846-6922], Kula-Pacurar, Anna [0000-0001-8404-2176], Pyrc, Krzysztof [0000-0002-3867-7688], Iraci, Nunzio [0000-0002-1359-8684], Santi, Claudio [0000-0002-7698-8970], Sancineto, Luca, Ostacolo, Carmine, Ortega-Alarcón, David, Jiménez-Alesanco, Ana, Ceballos-Laita, Laura, Vega, Sonia, Abian, Olga, Velázquez-Campoy, Adrián, Moretti, Silvia, Dabrowska, Agnieszka, Botwina, Pawel, Synowiec, Aleksandra, Kula-Pacurar, Anna, Pyrc, Krzysztof, Iraci, Nunzio, and Santi, Claudio

Abstract

The COVID-19 pandemic outbreak prompts an urgent need for efficient therapeutics, and repurposing of known drugs has been extensively used in an attempt to get to anti-SARS-CoV-2 agents in the shortest possible time. The glycoside rutin shows manifold pharmacological activities and, despite its use being limited by its poor solubility in water, it is the active principle of many pharmaceutical preparations. We herein report our in silico and experimental investigations of rutin as a SARS-CoV-2 Mpro inhibitor and of its water solubility improvement obtained by mixing it with l-arginine. Tests of the rutin/l-arginine mixture in a cellular model of SARS-CoV-2 infection highlighted that the mixture still suffers from unfavorable pharmacokinetic properties, but nonetheless, the results of this study suggest that rutin might be a good starting point for hit optimization.

Published
2021

2. Acriflavine, a clinically approved drug, inhibits SARS-CoV-2 and other betacoronaviruses

Author

Napolitano, Valeria, Dabrowska, Agnieszka, Schorpp, Kenji, Mourao, Andre, Barreto-Duran, Emilia, Benedyk, Malgorzata, Botwina, Pawel, Brandner, Stefanie, Bostock, Mark, Chykunova, Yuliya, Czarna, Anna, Dubin, Grzegorz, Froehlich, Tony, Hoelscher, Michael, Jedrysik, Malwina, Matsuda, Alex, Owczarek, Katarzyna, Pachota, Magdalena, Plettenburg, Oliver, Potempa, Jan, Rothenaigner, Ina, Schlauderer, Florian, Slysz, Klaudia, Szczepanski, Artur, Mohn, Kristin Greve-Isdahl, Blomberg, Bjorn, Sattler, Michael, Hadian, Kamyar, Popowicz, Grzegorz Maria, Pyrc, Krzysztof, Napolitano, Valeria, Dabrowska, Agnieszka, Schorpp, Kenji, Mourao, Andre, Barreto-Duran, Emilia, Benedyk, Malgorzata, Botwina, Pawel, Brandner, Stefanie, Bostock, Mark, Chykunova, Yuliya, Czarna, Anna, Dubin, Grzegorz, Froehlich, Tony, Hoelscher, Michael, Jedrysik, Malwina, Matsuda, Alex, Owczarek, Katarzyna, Pachota, Magdalena, Plettenburg, Oliver, Potempa, Jan, Rothenaigner, Ina, Schlauderer, Florian, Slysz, Klaudia, Szczepanski, Artur, Mohn, Kristin Greve-Isdahl, Blomberg, Bjorn, Sattler, Michael, Hadian, Kamyar, Popowicz, Grzegorz Maria, and Pyrc, Krzysztof

Abstract

The COVID-19 pandemic caused by SARS-CoV-2 has been socially and economically devastating. Despite an unprecedented research effort and available vaccines, effective therapeutics are still missing to limit severe disease and mortality. Using high-throughput screening, we identify acriflavine (ACF) as a potent papain-like protease (PLpro) inhibitor. NMR titrations and a co-crystal structure confirm that acriflavine blocks the PLpro catalytic pocket in an unexpected binding mode. We show that the drug inhibits viral replication at nanomolar concentration in cellular models, in vivo in mice and ex vivo in human airway epithelia, with broad range activity against SARS-CoV-2 and other betacoronaviruses. Considering that acriflavine is an inexpensive drug approved in some countries, it may be immediately tested in clinical trials and play an important role during the current pandemic and future outbreaks. © 2021 The Authors

Published
2022

3. In vivo confirmation of hydration-induced changes in human-skin thickness, roughness and interaction with the environment

Author

Dabrowska, Agnieszka K., Adlhart, Christian, Spano, Fabrizio, Rotaru, Gelu-Marius, Derler, Siegfried, Zhai, Lina, Spencer, Nicolas D., Rossi, René M., Dabrowska, Agnieszka K., Adlhart, Christian, Spano, Fabrizio, Rotaru, Gelu-Marius, Derler, Siegfried, Zhai, Lina, Spencer, Nicolas D., and Rossi, René M.

Abstract

Skin properties, structure and performance can be influenced by many internal and external factors, such as age, gender, lifestyle, skin diseases and a hydration level that can vary in relation to the environment. The aim of this work was to demonstrate the multifaceted influence of water on human skin through a combination of in vivo confocal Raman spectroscopy and images of volar-forearm skin captured with laser scanning confocal microscopy. By means of this pilot study, we have both qualitatively and quantitatively studied the influence of changing the depth-dependent hydration level of the stratum corneum (SC) on the real contact area (RCA), surface roughness and the dimensions of the primary lines and presented a new method for characterising the contact area for different states of the skin. The hydration level of the skin and the thickness of the SC increased significantly due to uptake of moisture derived from liquid water or, to a much lesser extent, from humidity present in the environment. Hydrated skin was smoother and exhibited higher RCA values. The highest rates of water uptake were observed for the upper few μm of skin and for short exposure times.

Published
2018

Catalog

Books, media, physical & digital resources
See catalog results