1. Apoptosis, Toll-like, RIG-I-like and NOD-like Receptors Are Pathways Jointly Induced by Diverse Respiratory Bacterial and Viral Pathogens
- Author
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Isidoro Martínez, Juan C. Oliveros, Isabel Cuesta, Jorge de la Barrera, Vicente Ausina, Casals Carro, María Cristina, Alba de Lorenzo, Ernesto García, García-Fojeda García-Valdecasas, María Belén, Junkal Garmendia, Mar González-Nicolau, Alicia Lacoma, Margarita Menéndez, David Moranta, Amelia Nieto, Juan Ortín, Alicia Pérez-González, Cristina Prat, Elisa Ramos-Sevillano, Verónica Regueiro, Ariel Rodriguez-Frandsen, Dolores Solís, José Yuste, José A. Bengoechea, José A. Melero, Isidoro Martínez, Juan C. Oliveros, Isabel Cuesta, Jorge de la Barrera, Vicente Ausina, Casals Carro, María Cristina, Alba de Lorenzo, Ernesto García, García-Fojeda García-Valdecasas, María Belén, Junkal Garmendia, Mar González-Nicolau, Alicia Lacoma, Margarita Menéndez, David Moranta, Amelia Nieto, Juan Ortín, Alicia Pérez-González, Cristina Prat, Elisa Ramos-Sevillano, Verónica Regueiro, Ariel Rodriguez-Frandsen, Dolores Solís, José Yuste, José A. Bengoechea, and José A. Melero
- Abstract
Lower respiratory tract infections are among the top five leading causes of human death. Fighting these infections is therefore a world health priority. Searching for induced alterations in host gene expression shared by several relevant respiratory pathogens represents an alternative to identify new targets for wide-range host-oriented therapeutics. With this aim, alveolar macrophages were independently infected with three unrelated bacterial (Streptococcus pneumoniae, Klebsiella pneumoniae, and Staphylococcus aureus) and two dissimilar viral (respiratory syncytial virus and influenza A virus) respiratory pathogens, all of them highly relevant for human health. Cells were also activated with bacterial lipopolysaccharide (LPS) as a prototypical pathogen-associated molecular pattern. Patterns of differentially expressed cellular genes shared by the indicated pathogens were searched by microarray analysis. Most of the commonly up-regulated host genes were related to the innate immune response and/or apoptosis, with Toll-like, RIG-I-like and NOD-like receptors among the top 10 signaling pathways with over-expressed genes. These results identify new potential broad-spectrum targets to fight the important human infections caused by the bacteria and viruses studied here., Instituto de Salud Carlos III, Ministerio de Economía y Competitividad, Sociedad Española de Neumología y Cirugía Torácica, European Union, Depto. de Bioquímica y Biología Molecular, TRUE, pub
- Published
- 2024