Search

In this paper, we study the possibility of inferring early warning indicators (EWIs) for periods of extreme bitcoin price volatility using features obtained from Bitcoin daily transaction graphs. We infer the low-dimensional representations of transaction graphs in the time period from 2012 to 2017 using Bitcoin blockchain, and demonstrate how these representations can be used to predict extreme price volatility events. Our EWI, which is obtained with a non-negative decomposition, contains more predictive information than those obtained with singular value decomposition or scalar value of the total Bitcoin transaction volume.

A novel utilization method of filter residue from the preparation process of zeolite-based catalysts was investigated. Y zeolite and a fluid catalytic cracking (FCC) catalyst were synthesized from filter residue. Compared to the Y zeolite synthesized by the conventional method, the Y zeolite synthesized from filter residue exhibited better thermal stability. The catalyst possessed wide-pore distribution. In addition, the pore volume, specific surface area, attrition resistance were superior to those of the reference catalyst. The yields of gasoline and light oil increased by 1.93 and 1.48 %, respectively. At the same time, the coke yield decreased by 0.41 %. The catalyst exhibited better gasoline and coke selectivity. The quality of the cracked gasoline had been improved., Proučena je upotreba taloga preostalog nakon priprave katalizatora baziranog na zeolitima. Od taloga su pripravljeni zeolit Y i katalizator za katalitičko krekiranje u fluidiziranom sloju (FCC). Ovako sintetiziran zeolit Y termički je stabilniji u odnosu na zeolit Y sintetiziran konvencionalnom metodom. Katalizator ima široku distribuciju veličine pora. U odnosu na referentni katalizator pokazuje veći obujam pora i specifičnu površinu te bolju otpornost na atriciju. Iskorištenje benzina i lakog ulja povećalo se za 1,93 i 1,48 %, a koksa smanjilo za 0,41 %. Katalizator je pokazao bolju selektivnost s obzirom na benzin i koks, a poboljšala se kvaliteta krekiranog benzina.

Recent evidence supports the idea that early aggregates, protein, and lipoprotein oligomers but not large aggregates like fibrils that are formed at late stages of the aggregation process are responsible for cytotoxicity. Oligomers can interact with the cellular plasma membrane affecting its structure and/or dynamics or may be taken up by the cells. In either case, disparate cascades of molecular interactions are activated in the attempt to counteract the disturbance induced by the oligomers. If unsuccessful, cell death follows. Here, we study the molecular and cellular mechanisms underlying PC12 cell death caused by ELOA oligomers. ELOA, a lipoprotein complex formed by equine lysozyme (EL) and oleic acid (OA), induces cell death in all tested cell lines, but the actual mechanism of its action is not known. We have used methods with single-molecule sensitivity, fluorescence correlation spectroscopy (FCS), fluorescence cross-correlation spectroscopy (FCCS), and confocal laser scanning microscopy (CLSM) imaging by avalanche photodiodes (APD), so-called APD imaging, to study ELOA interactions with the plasma membrane in live PC12 cells. We detected ELOA accumulation in the cell surroundings, observed ELOA interactions with the plasma membrane, and local changes in plasma membrane lipid dynamics in the vicinity of ELOA complexes. These interactions resulted in plasma membrane rupture, followed by rapid influx and distribution of ELOA inside the already dead cell. In order to probe the ELOA−plasma membrane interaction sites at the molecular and atomic levels, the ELOA complexes were further studied by photochemically induced dynamic nuclear polarization (photo-CIDNP) spectroscopy, nuclear magnetic resonance (NMR) and atomic force microscopy (AFM). We observed a novel mechanism of oligomer toxicity−cell death induced by continuous disturbance of the plasma membrane, eventually causing permanent plasma membrane damage and identified the sites in ELOA that are potentially i