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Mucosal-associated invariant T (MAIT) cells are a subset of unconventional T cells which recognize a limited repertoire of ligands presented by the MHC class-I like molecule MR1. In addition to their key role in host protection against bacterial and viral pathogens, MAIT cells are emerging as potent anti-cancer effectors. With their abundance in human, unrestricted properties, and rapid effector functions MAIT cells are emerging as attractive candidates for immunotherapy. In the current study, we demonstrate that MAIT cells are potent cytotoxic cells, rapidly degranulating and inducing target cell death. Previous work from our group and others has highlighted glucose metabolism as a critical process for MAIT cell cytokine responses at 18 h. However, the metabolic processes supporting rapid MAIT cell cytotoxic responses are currently unknown. Here, we show that glucose metabolism is dispensable for both MAIT cell cytotoxicity and early (<3 h) cytokine production, as is oxidative phosphorylation. We show that MAIT cells have the machinery required to make (GYS-1) and metabolize (PYGB) glycogen and further demonstrate that that MAIT cell cytotoxicity and rapid cytokine responses are dependent on glycogen metabolism. In summary, we show that glycogen-fueled metabolism supports rapid MAIT cell effector functions (cytotoxicity and cytokine production) which may have implications for their use as an immunotherapeutic agent.

For the past year, the Discovery Committee has been working on creating a new search interface for the libraries using NCSU Libraries' QuickSearch, an open-source application. This poster will provide an update on the progress the group has made and will include a live demo and usability testing of the in-development application.

Pickering, C, Suraci, B, Semenova, EA, Boulygina, EA, Kostryukova, ES, Kulemin, NA, Borisov, OV, Khabibova, SA, Larin, AK, Pavlenko, AV, Lyubaeva, EV, Popov, DV, Lysenko, EA, Vepkhvadze, TF, Lednev, EM, Leońska-Duniec, A, Pająk, B, Chycki, J, Moska, W, Lulińska-Kuklik, E, Dornowski, M, Maszczyk, A, Bradley, B, Kana-ah, A, Cięszczyk, P, Generozov, EV, and Ahmetov, II. A genome-wide association study of sprint performance in elite youth football players. J Strength Cond Res XX(X): 000-000, 2019-Sprint speed is an important component of football performance, with teams often placing a high value on sprint and acceleration ability. The aim of this study was to undertake the first genome-wide association study to identify genetic variants associated with sprint test performance in elite youth football players and to further validate the obtained results in additional studies. Using micro-array data (600 K-1.14 M single nucleotide polymorphisms [SNPs]) of 1,206 subjects, we identified 12 SNPs with suggestive significance after passing replication criteria. The polymorphism rs55743914 located in the PTPRK gene was found as the most significant for 5-m sprint test (p = 7.7 × 10). Seven of the discovered SNPs were also associated with sprint test performance in a cohort of 126 Polish women, and 4 were associated with power athlete status in a cohort of 399 elite Russian athletes. Six SNPs were associated with muscle fiber type in a cohort of 96 Russian subjects. We also examined genotype distributions and possible associations for 16 SNPs previously linked with sprint performance. Four SNPs (AGT rs699, HSD17B14 rs7247312, IGF2 rs680, and IL6 rs1800795) were associated with sprint test performance in this cohort. In addition, the G alleles of 2 SNPs in ADRB2 (rs1042713 & rs1042714) were significantly over-represented in these players compared with British and European controls. These results suggest that there is a genetic influence on sprint test performance in footballers

This document describes the work performed as part of the Ananke simulation development. The Ananke simulation tool development is a larger project to provide end-to-end space object simulation and characterization to demonstrate the feasibility of such methods. This document describes the orbit propagation tool, CO-TurboProp, used in the Ananke tool. The document describes the addition of higher-fidelity models in CO-TurboProp and validation of the new tools. The description provided here includes the mathematical background and description of the models implemented, as well as a definition of the software interface for the larger Ananke tool. Comparisons of the software with semi-analytic techniques and other available high-fidelity propagation tools are also presented, and all tools met all required and expected accuracies., The original document contains color images.

This chapter examines the connection between value and desire with regard to death. It argues that having categorical desires is a necessary condition for death to be bad for those who die, and that the degree to which death is bad bears a close relation to the number and strength of those desires. The chapter also analyzes the principles espoused by Jeff McMahan in his book “The Ethics of Killing: Problems at the Margins of Life.”.

This chapter examines the connection between value and desire with regard to death. It argues that having categorical desires is a necessary condition for death to be bad for those who die, and that the degree to which death is bad bears a close relation to the number and strength of those desires. The chapter also analyzes the principles espoused by Jeff McMahan in his book “The Ethics of Killing: Problems at the Margins of Life.”.