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We detected HBV-DNA in two out of 15 (13.3%) archival liver biopsies from Saudi HCC patients. Our real-time quantitative assay of HBV by SYBR green was more accurate and has detection range from 102 to 1010 copies/ml. The melting temperature varied 80.5 - 81.7 oC in different genotypes. Mean HBV DNA level was significantly higher in UK than Saudi hepatitis B patients at 5.09 (95% CI 1.43-8.75) and 4.52 (95% CI 0.14-8.90) log10 copies/ml, respectively (p: 0.007). Mean HBV DNA in patients with HBeAg was higher at 7.18 and 6.44 log10 copies/ml in UK and Saudi patients, respectively. HBV genotypes A, B, C and D were mostly detected in UK patients, A and D in Saudi patients. In UK patients, genotypes C and D showed higher mean HBV DNA than genotypes A or B. Saudi patients had higher mean HBV DNA in genotypes D than genotypes A or B. Within genotype D, UK patients had even higher HBV DNA levels than Saudi patients at 6.10 and 5.10 log10 IU/ml, respectively. All UK HBV genotype A had subgenotype Ae, genotype B had subgenotype Ba, and genotype C subgenotype C1 (Cs). HBV detected in Saudi Samples had HBV subgenotype D1, while UK had subgenotype D2 mostly in Asian patients. Core promoter region/X gene sequences showed clear correlation to HBV genotypes and subgenotypes unlike the conserved precore region. Sequencing of the S gene, Pre S1 region, Pre S2 and beginning as well as the end of S gene overlapping the YMDD region of the reverse transcriptase part of pol gene showed accurate discrimination between genotypes, and genotype-related sequence variations. In contrast, the more conserved regions at the `a` determinant and the rt part of the Pol gene. That can contribute to differences in fibronectin binding site to liver sinusoids, and the expression efficiency of HBsAg between different genotypes. Furthermore, the genotype-specific contribution of preS1 epitopes favours vaccination using the large HBsAg of the prevalent HBV genotypes in specific geographical areas. We found

We detected HBV-DNA in two out of 15 (13.3%) archival liver biopsies from Saudi HCC patients. Our real-time quantitative assay of HBV by SYBR green was more accurate and has detection range from 102 to 1010 copies/ml. The melting temperature varied 80.5 - 81.7 oC in different genotypes. Mean HBV DNA level was significantly higher in UK than Saudi hepatitis B patients at 5.09 (95% CI 1.43-8.75) and 4.52 (95% CI 0.14-8.90) log10 copies/ml, respectively (p: 0.007). Mean HBV DNA in patients with HBeAg was higher at 7.18 and 6.44 log10 copies/ml in UK and Saudi patients, respectively. HBV genotypes A, B, C and D were mostly detected in UK patients, A and D in Saudi patients. In UK patients, genotypes C and D showed higher mean HBV DNA than genotypes A or B. Saudi patients had higher mean HBV DNA in genotypes D than genotypes A or B. Within genotype D, UK patients had even higher HBV DNA levels than Saudi patients at 6.10 and 5.10 log10 IU/ml, respectively. All UK HBV genotype A had subgenotype Ae, genotype B had subgenotype Ba, and genotype C subgenotype C1 (Cs). HBV detected in Saudi Samples had HBV subgenotype D1, while UK had subgenotype D2 mostly in Asian patients. Core promoter region/X gene sequences showed clear correlation to HBV genotypes and subgenotypes unlike the conserved precore region. Sequencing of the S gene, Pre S1 region, Pre S2 and beginning as well as the end of S gene overlapping the YMDD region of the reverse transcriptase part of pol gene showed accurate discrimination between genotypes, and genotype-related sequence variations. In contrast, the more conserved regions at the `a` determinant and the rt part of the Pol gene. That can contribute to differences in fibronectin binding site to liver sinusoids, and the expression efficiency of HBsAg between different genotypes. Furthermore, the genotype-specific contribution of preS1 epitopes favours vaccination using the large HBsAg of the prevalent HBV genotypes in specific geographical areas. We found

We detected HBV-DNA in two out of 15 (13.3%) archival liver biopsies from Saudi HCC patients. Our real-time quantitative assay of HBV by SYBR green was more accurate and has detection range from 102 to 1010 copies/ml. The melting temperature varied 80.5 - 81.7 oC in different genotypes. Mean HBV DNA level was significantly higher in UK than Saudi hepatitis B patients at 5.09 (95% CI 1.43-8.75) and 4.52 (95% CI 0.14-8.90) log10 copies/ml, respectively (p: 0.007). Mean HBV DNA in patients with HBeAg was higher at 7.18 and 6.44 log10 copies/ml in UK and Saudi patients, respectively. HBV genotypes A, B, C and D were mostly detected in UK patients, A and D in Saudi patients. In UK patients, genotypes C and D showed higher mean HBV DNA than genotypes A or B. Saudi patients had higher mean HBV DNA in genotypes D than genotypes A or B. Within genotype D, UK patients had even higher HBV DNA levels than Saudi patients at 6.10 and 5.10 log10 IU/ml, respectively. All UK HBV genotype A had subgenotype Ae, genotype B had subgenotype Ba, and genotype C subgenotype C1 (Cs). HBV detected in Saudi Samples had HBV subgenotype D1, while UK had subgenotype D2 mostly in Asian patients. Core promoter region/X gene sequences showed clear correlation to HBV genotypes and subgenotypes unlike the conserved precore region. Sequencing of the S gene, Pre S1 region, Pre S2 and beginning as well as the end of S gene overlapping the YMDD region of the reverse transcriptase part of pol gene showed accurate discrimination between genotypes, and genotype-related sequence variations. In contrast, the more conserved regions at the `a` determinant and the rt part of the Pol gene. That can contribute to differences in fibronectin binding site to liver sinusoids, and the expression efficiency of HBsAg between different genotypes. Furthermore, the genotype-specific contribution of preS1 epitopes favours vaccination using the large HBsAg of the prevalent HBV genotypes in specific geographical areas. We found

We detected HBV-DNA in two out of 15 (13.3%) archival liver biopsies from Saudi HCC patients. Our real-time quantitative assay of HBV by SYBR green was more accurate and has detection range from 102 to 1010 copies/ml. The melting temperature varied 80.5 - 81.7 oC in different genotypes. Mean HBV DNA level was significantly higher in UK than Saudi hepatitis B patients at 5.09 (95% CI 1.43-8.75) and 4.52 (95% CI 0.14-8.90) log10 copies/ml, respectively (p: 0.007). Mean HBV DNA in patients with HBeAg was higher at 7.18 and 6.44 log10 copies/ml in UK and Saudi patients, respectively. HBV genotypes A, B, C and D were mostly detected in UK patients, A and D in Saudi patients. In UK patients, genotypes C and D showed higher mean HBV DNA than genotypes A or B. Saudi patients had higher mean HBV DNA in genotypes D than genotypes A or B. Within genotype D, UK patients had even higher HBV DNA levels than Saudi patients at 6.10 and 5.10 log10 IU/ml, respectively. All UK HBV genotype A had subgenotype Ae, genotype B had subgenotype Ba, and genotype C subgenotype C1 (Cs). HBV detected in Saudi Samples had HBV subgenotype D1, while UK had subgenotype D2 mostly in Asian patients. Core promoter region/X gene sequences showed clear correlation to HBV genotypes and subgenotypes unlike the conserved precore region. Sequencing of the S gene, Pre S1 region, Pre S2 and beginning as well as the end of S gene overlapping the YMDD region of the reverse transcriptase part of pol gene showed accurate discrimination between genotypes, and genotype-related sequence variations. In contrast, the more conserved regions at the `a` determinant and the rt part of the Pol gene. That can contribute to differences in fibronectin binding site to liver sinusoids, and the expression efficiency of HBsAg between different genotypes. Furthermore, the genotype-specific contribution of preS1 epitopes favours vaccination using the large HBsAg of the prevalent HBV genotypes in specific geographical areas. We found

We detected HBV-DNA in two out of 15 (13.3%) archival liver biopsies from Saudi HCC patients. Our real-time quantitative assay of HBV by SYBR green was more accurate and has detection range from 102 to 1010 copies/ml. The melting temperature varied 80.5 - 81.7 oC in different genotypes. Mean HBV DNA level was significantly higher in UK than Saudi hepatitis B patients at 5.09 (95% CI 1.43-8.75) and 4.52 (95% CI 0.14-8.90) log10 copies/ml, respectively (p: 0.007). Mean HBV DNA in patients with HBeAg was higher at 7.18 and 6.44 log10 copies/ml in UK and Saudi patients, respectively. HBV genotypes A, B, C and D were mostly detected in UK patients, A and D in Saudi patients. In UK patients, genotypes C and D showed higher mean HBV DNA than genotypes A or B. Saudi patients had higher mean HBV DNA in genotypes D than genotypes A or B. Within genotype D, UK patients had even higher HBV DNA levels than Saudi patients at 6.10 and 5.10 log10 IU/ml, respectively. All UK HBV genotype A had subgenotype Ae, genotype B had subgenotype Ba, and genotype C subgenotype C1 (Cs). HBV detected in Saudi Samples had HBV subgenotype D1, while UK had subgenotype D2 mostly in Asian patients. Core promoter region/X gene sequences showed clear correlation to HBV genotypes and subgenotypes unlike the conserved precore region. Sequencing of the S gene, Pre S1 region, Pre S2 and beginning as well as the end of S gene overlapping the YMDD region of the reverse transcriptase part of pol gene showed accurate discrimination between genotypes, and genotype-related sequence variations. In contrast, the more conserved regions at the `a` determinant and the rt part of the Pol gene. That can contribute to differences in fibronectin binding site to liver sinusoids, and the expression efficiency of HBsAg between different genotypes. Furthermore, the genotype-specific contribution of preS1 epitopes favours vaccination using the large HBsAg of the prevalent HBV genotypes in specific geographical areas. We found

We detected HBV-DNA in two out of 15 (13.3%) archival liver biopsies from Saudi HCC patients. Our real-time quantitative assay of HBV by SYBR green was more accurate and has detection range from 102 to 1010 copies/ml. The melting temperature varied 80.5 - 81.7 oC in different genotypes. Mean HBV DNA level was significantly higher in UK than Saudi hepatitis B patients at 5.09 (95% CI 1.43-8.75) and 4.52 (95% CI 0.14-8.90) log10 copies/ml, respectively (p: 0.007). Mean HBV DNA in patients with HBeAg was higher at 7.18 and 6.44 log10 copies/ml in UK and Saudi patients, respectively. HBV genotypes A, B, C and D were mostly detected in UK patients, A and D in Saudi patients. In UK patients, genotypes C and D showed higher mean HBV DNA than genotypes A or B. Saudi patients had higher mean HBV DNA in genotypes D than genotypes A or B. Within genotype D, UK patients had even higher HBV DNA levels than Saudi patients at 6.10 and 5.10 log10 IU/ml, respectively. All UK HBV genotype A had subgenotype Ae, genotype B had subgenotype Ba, and genotype C subgenotype C1 (Cs). HBV detected in Saudi Samples had HBV subgenotype D1, while UK had subgenotype D2 mostly in Asian patients. Core promoter region/X gene sequences showed clear correlation to HBV genotypes and subgenotypes unlike the conserved precore region. Sequencing of the S gene, Pre S1 region, Pre S2 and beginning as well as the end of S gene overlapping the YMDD region of the reverse transcriptase part of pol gene showed accurate discrimination between genotypes, and genotype-related sequence variations. In contrast, the more conserved regions at the `a` determinant and the rt part of the Pol gene. That can contribute to differences in fibronectin binding site to liver sinusoids, and the expression efficiency of HBsAg between different genotypes. Furthermore, the genotype-specific contribution of preS1 epitopes favours vaccination using the large HBsAg of the prevalent HBV genotypes in specific geographical areas. We found