1. DPP-4 Inhibitor Linagliptin Ameliorates Oxidized LDL-Induced THP-1 Macrophage Foam Cell Formation and Inflammation
- Author
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Wang,Haoran, Li,Yue, Zhang,Xiaoliang, Xu,Zhonglin, Zhou,Jianzhong, Shang,Wei, Wang,Haoran, Li,Yue, Zhang,Xiaoliang, Xu,Zhonglin, Zhou,Jianzhong, and Shang,Wei
- Abstract
Haoran Wang,1 Yue Li,2 Xiaoliang Zhang,2 Zhonglin Xu,2 Jianzhong Zhou,3 Wei Shang2 1Department of Endocrinology, The Ninth People’s Hospital of Chongqing, Chongqing 400700, People’s Republic of China; 2Department of Cardiology, The Ninth People’s Hospital of Chongqing, Chongqing 400700, People’s Republic of China; 3Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400700, People’s Republic of ChinaCorrespondence: Wei ShangDepartment of Cardiology, The Ninth People’s Hospital of Chongqing, No. 69, Jia Ling Road, Bei Bei District, Chongqing 400700, People’s Republic of ChinaTel +86-15823409229Email wshang355@163.comIntroduction: Atherosclerosis is one of the major causes of cardiovascular diseases. Lipid uptake and accumulation in macrophages play a major role in atherosclerotic plaque formation from its initiation to advanced atheroma formation. The dipeptidyl peptidase-4 (DPP-4) inhibitor Linagliptin is commonly used to lower blood glucose in type 2 diabetes patients. Recent studies report that Linagliptin has cardiovascular protective and anti-inflammatory effects.Methods: THP-1 macrophage cells were treated with 100 nM PMA for 72 hour to induce foam cell formation. The differentiated cells were exposed to 100 μg/mL ox-LDL in the presence or absence of the DPP-4 inhibitor Linagliptin. The expression levels of DPP-4 and inflammatory cytokines were detected by RT-PCR, ELISA, and Western blot experiments. The cellular ROS level was measured by staining the cells with the fluorescent probe DCFH-DA. The separation of lipoprotein fractions was achieved by high-performance liquid chromatography (HPLC). The cells were labeled with fluorescent-labeled cholesterol to measure cholesterol efflux, and lipid droplets were revealed by Nile red staining.Results: The presence of Linagliptin significantly reduced ox-LDL-induced cytokine production (IL-1&b more...
- Published
- 2020