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Effective spiritual care is difficult to define and evaluate objectively. As chaplains and Religious Support Teams endeavor to provide care in the highly mission-focused and objective-driven context of the United States military, this difficulty becomes uniquely problematic. The author demonstrates this reality using an exemplar Air Force Reserve chapel team as a case study. Blending transformational leadership, situational leadership, and John Kotter’s change model, the author constructs a vision for change culminating in a base level Operating Instruction that offers specific objectives, measurable tasks, and qualification metrics. Alongside this instruction, he crafts an Implementation Strategy designed to strategically inspire and motivate teams toward change. In doing so, he provides a path forward to allow chaplains to not only evaluate ministry effectiveness but also enhance communication with leaders at the base level and up into the highest levels of the Department of Defense.

DNA damage by reactive oxygen species (ROS) is a cause of many chronic diseases. This work examines the ability of sulfur and selenium antioxidants to prevent oxidative DNA damage and the mechanisms for this activity. Although iron- and copper-generated hydroxyl radical are primary causes of damage under oxidative stress conditions, studies typically focus on ROS scavenging rather than antioxidant-metal binding as a mechanism for sulfur and selenium antioxidant behavior. Mass spectrometry studies of sulfur and selenoamino acids (Chapter 2) show that most form CuI and FeII complexes, regardless of their metal-mediated DNA damage prevention abilities. Because their electrochemical properties do not correlate to antioxidant activity, metal binding rather than ROS scavenging is the major mechanism for these sulfur and selenium antioxidants. DNA damage assays with N,N’-dimethylimazole thione (dmit) and selone (dmise) determined that both prevent CuI-mediated DNA damage (IC50 = 1550 and ~240 µM, respectively; Chapter 3). Surprisingly, dmit and dmise more effectively inhibit FeII-mediated DNA damage (IC50 = 89.1 and 3.2 µM, respectively), an ability not previously observed for this class of antioxidants. Dmise and dmit coordinate CuI and FeII and prevent DNA damage by peroxynitrite (IC50 = 171.4 and 155.2, respectively). Studies with similar thiones, selones, and their derivatives (Chapter 4) showed that these compounds are also multifunctional antioxidants, preventing DNA damage by CuI (IC50= 22-1023 µM), FeII (IC50 = 2.3-1000 µM) and peroxynitrite (IC50 = 57.4-594 µM). Many of these compounds readily undergo oxidation and reduction, and mass spectrometry studies show CuI or FeII coordination, regardless of antioxidant activity. These are the first sulfur and selenium compounds with multifunctional antioxidant activity, and the structure-activity relationships established in this work will allow development of more potent antioxidants for disease treatment and prevention. Studies in Chapter 5 focus on how metal binding alters drug properties. Clotrimazole-metal complexes kill cancer cells, yet their cytotoxic mechanisms are not understood. Similarly, studies have not examined the effects of metal coordination on the biological properties of pseudoephedrine-derived compounds. DNA damage studies with copper complexes of both compounds found that they significantly damage DNA (EC50 = 10.5-21.7 µM), likely by copper-mediated ROS generation.