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The key to understanding protein dynamics, localizations, and macromolecular architectures lies largely in intra-cellular visualization. Many of the limitations we face today are due to our inability to adequately label our protein of interest. Herein we describe solutions to some of these barriers through an improved super-resolution labeling technique. We further use our newly elaborated knock-in strategies to investigate the kinetics of endogenous protein kinase A (PKA) responses to beta-adrenergic signaling, and investigate newly observed recruitment of the endogenous kinase.