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To comply with the European Union Water Framework Directive (2000), National Governments are required to achieve good chemical and ecological status of freshwater bodies. Fine sediment has been shown to be a major cause of the degradation of lakes and rivers, and as a result research in geomorphology has been directed towards the understanding of fine sediment dynamics. It was identified by a review of published literature that at present a paucity of information on sediment dynamics existed for the East Midlands, UK. The use of tracers within a sediment fingerprinting framework has recently become a heavily used technique to investigate the sources of fine sediment pressures. However, uncertainties associated with tracer behaviour have been cited as major potential limitations to sediment fingerprinting methodologies. At present few studies have quantified the uncertainties associated with tracer use, or the exact reasons why different tracers are producing different sediment provenance results. This thesis had two aims based on these gaps in published literature. First, to assess the impact of sediment sampling methodology, tracer selection, particle size corrections and organic enrichment corrections on a fine sediment fingerprinting study. Secondly, to develop a partial sediment budget for the Upper Nene river basin and its major tributaries. The results of this thesis were presented in two parts. The first part investigated Aim 1 when fingerprinting; historically deposited sediment, suspended sediment and recently deposited sediment. The second part investigated Aim 2 by constructing a fine sediment budget for the Nene river basin, consisting of; sediment yield, sediment provenance, floodplain sediment accumulation and channel bed sediment storage. A mean difference of 24.1% between the predicted contributions of sediment originating from channel banks was found when using nine different tracer groups to fingerprint the river sediment samples. When fingerprinting contributions from urban street dusts mean differences between tracer group predictions were lower, at between 8% and 11%. There was little indication that organic matter content and / or particle size caused differences between tracer group predictions. Within-source variability in tracer concentrations, and small contrasts between the tracer concentrations of the sediment source groups, were identified as probable causes of inherent uncertainty present in the fingerprinting analysis. It was determined that the ratio of the percentage difference between median tracer concentrations in the source groups and the average within-source tracer concentration coefficient of variation could indicate the likely uncertainty in model predictions prior to tracer use. When fingerprinting historically deposited sediment, a reservoir core was fingerprinted with the least uncertainty, with tracer group provenance predictions ~28% apart and with consistent down-core trends. When fingerprinting an on-line lake core and four floodplain cores, differences between tracer group predictions were as large as 100%; the down-core trends in changing sediment provenance were also different. The differences between tracer group predictions could be attributed to the organic matter content and particle size of the sediment. There was also evidence of the in-growth of bacterially derived magnetite and chemical dissolution affecting the preservation of tracer signatures. Despite the prior indications that organic matter and particle size were causing tracer non-conservatism in historical sediment cores, data corrections were found to often be ineffective at reducing the differences between tracer group predictions. The corrections were found to either have no effect on, or increase the mean differences between, tracer group predictions when fingerprinting river sediment. The sediment budget identified that the annual sediment yield of 13 - 19 t km-2 yr-1 for the Nene is low in comparison to other UK catchments. Channel banks were found to be the dominant sediment source in the Nene, typically contributing between 60% and 100% of the sediment. Rates of sediment accumulation on the Nene’s floodplain was found to be highly variable (920– 7,200 t km-2 yr-1); the presence of flood defences were likely to be a cause of this variability, and have caused a reduction in the accumulation rate since 1963. It was found that large quantities of sediment accumulated on channel beds during periods of low flows (~ 28% of the annual sediment yield), which was flushed from the bed by a series of flood events (leaving <1% of the annual sediment yield in temporary storage). An original contribution to research was made by quantifying the uncertainties associated with tracer use in a fine sediment fingerprinting investigation, as well as identifying the probable causes of the observed uncertainty. The fine sediment dynamics of the Nene basin were also investigated for the first time, and it was identified that the high contributions from channel banks in the Nene were highly a-typical for UK catchments.

Hides and skins used as a raw material for leather manufacture may be contaminated with various microbial species including potential pathogens. Many bacterial species such as Bacillus, Staphylococcus, Micrococcus and Pseudomonas were isolated from raw hides/skins, and hides/skins at different stages of leather making process. The extreme environmental conditions present during a conventional tanning process due to hazardous chemicals may prevent the growth of bacteria present on hides/skins. On the other hand, partial or total replacement of the hazardous chemicals with non-hazardous chemicals, during a best available technologies (BAT) process, may provide suitable conditions for microbial growth in tannery effluent and hides/skins. Therefore, the aim of the project was to determine the survival and growth of the various bacterial species during the conventional and BAT leather-making processes. The beamhouse and tanning stages were studied, as the majority of the environmental pollution occurs during the early stages of the leather making process. Both the pre-soaking and soaking stages during the conventional and BAT leather-making processes provided suitable conditions for bacterial (Bacillus cereus, Pseudomonas aeruginosa and Staphylococcus spp.) growth and proliferation. The results showed a significant reduction in the number of B. cereus found during the conventional and BAT unhairing processes. Limited B. cereus growth was observed during the subsequent reliming process. Bacillus cereus growth also occurred during the deliming and bating processes (conventional and BAT), followed by a decrease during the conventional and BAT pickling processes. No B. cereus colonies were isolated during the chrome tanning process. Growth of P. aeruginosa was inhibited during both the unhairing and reliming stages of the conventional and BAT leather making processes. A reappearance and recovery of P. aeruginosa in the subsequent deliming and bating (conventional and BAT) processes, indicated that deliming and bating processes may provide suitable growth conditions for P. aeruginosa. On the other hand, both the conventional and BAT pickling processes, and the chrome tanning processes hindered P. aeruginosa growth. Staphylococcus spp. were present throughout the conventional and BAT leather-making processes. A large reduction in the number of Staphylococcus spp. was observed during the unhairing and reliming processes (conventional and BAT). Growth of Staphylococcus spp. occurred during the subsequent deliming, bating, pickling and chrome tanning stages for both the conventional and BAT leather-making processes. The biochemical assays for bacterial identification confirmed the presence of B. cereus, P. aeruginosa and Staphylococcus spp. during the leather processing. The pulsed-field gel electrophoresis (PFGE) method of DNA fingerprinting confirmed that the bacterial species isolated during the leather manufacturing processes were the inoculated B. cereus and P. aeruginosa, and no alteration of the DNA of above-mentioned bacteria occurred during the processing. Overall, the research showed that bacterial species are capable of surviving during both the conventional and BAT leather-manufacturing processes. The bacterial species prefer the environmental conditions during the pre-soaking and soaking processes, while the unhairing and reliming processes did not favour the growth of bacterial species. Bacterial colonies were enumerated during the deliming and bating processes indicating that the unhairing and reliming processes did not cause total destruction of the bacterial cells. Alternatively pickling and chrome tanning processes were found to have suppressed the growth of bacterial colonies.

The most significant problem of the tanning industry is waste generation. The presence of chromium in leather waste represents a potential hazard due to the possibility of Cr(III) being converted to its toxic state, Cr(VI). The treatment of chrome-tanned leather waste described in this research occurs in three phases: dechroming; isolation of gelatin, and application of the gelatin produced in the finishing of leather. Chrome-tanned shavings were dechromed and subsequently used for the production of gelatin. Gelatin was isolated from dechromed shavings in a one-step process, through thermal and chemical hydrolysis, using the hydrolytic agents ammonia, sodium hydroxide and acetic acid. The gelatin obtained through thermal hydrolysis exhibited better properties, in terms of gel strength, melting point, swelling and thermal properties, than the gelatin obtained through chemical hydrolysis and the former process was more cost-effective in terms of time, chemicals and waste production. The use of gelatin may be limited by its poor mechanical properties. The introduction of chemical modifications, using the crosslinking agents glutaraldehyde (GTA), oxazolidine II, ethylene glycol cliglycidyl ether (epoxy compound), hexamethylene dii socyanate (HMDC) and I -ethyl-3 -(3 -dimethylaminopropyl) carbodiimide (EDC), improved the performance in terms of strength and stability of the gelatin: oxazolidine and ethylene glycol diglycidyl ether are the most suitable since they confer plasticising properties in addition to stabilising the gelatin. A plasticiser (glycerol) was added into the matrix network of gelatin, increasing its flexibility. The isolated gelatin and a mixture of gelatinlglycerol were compared in use as a replacement for the commercial protein binder, casein, for glazed finishing. The finishing formulations were crosslinked with epoxy compound and oxazolidine. Physical tests carried out on the finished product showed promising results, with the gelatin/epoxy formulation showing potential for application. Ihe potential fbr gelatin isolated from chrome shavings to he produced as a high-added value material lor new applications in the future, such as a component tor finishing Formulations in the leather industry, was demonstrated

Wound dressing plays an important role in wound recovery and collagen interacts with the human body in such a way that it has specific advantages compared to synthetic materials. The aim of the present study was to get an optimal crosslinking agent for collagen and so the mechanical, chemical and biochemical properties of crosslinked collagen materials were investigated. Fibroblast cells are important in the process of wound healing, so the interaction of human fibroblast cells with crosslinked collagen films were investigated as well. Collagen I was isolated from bovine achilles tendons and collagen films were formed using the isolated collagen I solution. Collagen films were crosslinked with glutaraldehyde (GA), genipin, hexamethylenediisocyanate (HMDC), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) at the equal concentration of 0.02 M and these crosslinked collagen films were compared with uncrosslinked collagen films (control). The surfaces of the crosslinked films were investigated using scanning electron microscopy (SEM). There was observable fibre structure on GA- and genipin-crosslinked collagen films. The tensile strength, elongation at break and low strain modulus of the crosslinked collagen films were investigated. The results showed that GA-, genipin- and HMDC-crosslinked collagen films obtained higher tensile strength than the control. Elongation at break of all the crosslinked collagen films became lower than the control. GA- and genipin-crosslinked collagen films obtained higher low strain modulus than other crosslinked collagen films and the control. The denaturation temperatures of all crosslinked collagen films were significantly higher than the control and the denaturation temperatures of GA- and genipin-crosslinked films were much higher than those of HMDC- and EDC-crosslinked films. All the crosslinked collagen films were resistant to the digestion of collagenase. These results suggest that all the crosslinking agents are effective and GA- and genipin-crosslinked films obtained more extensive crosslinking. The interaction of crosslinked collagen films with fibroblast cells was investigated, e.g. adhesion, proliferation and migration of fibroblast cells. The results demonstrated that the control, genipin- and EDC-crosslinked collagen films were conducive to cell adhesion. Fibroblast cells on the control, genipin- and EDC-crosslinked collagen films were able to proliferate after 24 hours, with increased growth after 48 hours. The fibroblast cells on the control, genipin- and EDC-crosslinked collagen films migrated directionally. The cells on genipin-crosslinked film initiated directional migration earlier than those on control- and EDC-crosslinked films. In summary, genipin crosslinked collagen films show high denaturation temperature, higher tensile strength and good biocompatibility for fibroblast cells adhesion, proliferation and migration. Genipin should be regarded as a suitable crosslinking agent for reconstituted collagen for use in wound dressing.