Wheat is the major field crop grown in Latvia. High quality wheat grains are required for the milling and baking industries. Gluten quantity and quality are important qualities indices for technological processing of wheat. Our objectives were to determine relative influence of variety (V), environment (E), nitrogen fertilizer (N) on variation winter wheat (Triticum aestivum. L) of gluten quantity and quality indices. Field experiments with 9 winter wheat varieties with different origin were conducted on brown lessive soils of the Study and Research farm “Peterlauki” of the Latvia University of Agriculture in 2000, 2001, 2002 and 2004. Split nitrogen fertilization was applied in the following way: early in spring at the beginning of vegetation period, at the end of tillering and at the end of shooting into stems. The N fertilizer amount applied was four nitrogen fertilizer (90, 90+30, 90+60 and 90+60+30 N kg ha-1) treatments for all the studied winter wheat varieties. Wet gluten content (WG), gluten index (GI), dry gluten (DG), water binding capacity in wet gluten (WBC) were measured at Latvia University of Agriculture, Institute of Agrobiotehnology, Grain and Seed Research Laboratory by ICC No. 155 and. No. 137 /1 (LV ST-275). Highly significant differences were detected among the environments, N fertilizer and varieties for each of the quality variables. Variety and environment and nitrogen fertilizer had a significant effect on wet gluten quality indices. Our results show, that gluten quantity and quality indices were mostly influenced by genetic peculiarities of a crop variety, in less extent by meteorological conditions in the growing season and by the rate of split N fertilizer. Close positive correlations were determined between the wet gluten and dry gluten, water binding capacity and dry gluten, water binding capacity and wet gluten. [ABSTRACT FROM AUTHOR]