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Few studies have extensively reported the biochemical changes in paddle competition. The aim of this study is analyze detailed changes in twenty biochemistry test parameters before and after paddle matches. Sixteen elite female paddle players (age 29.8 ± 4.5years; height 166.5 ± 4.9cm; body weight 60.3 ± 4.3kg; BMI 20.5 ± 1.0) were studied during competitive matches. Significant changes were observed (p < 0.05) between the pre- and post-competition samples in some biochemical variables: glucose, urea, creatinine, uric acid, sodium, potassium, chloride, phosphorus, and magnesium, aspartate aminotransferase, creatine kinase and lactate dehydrogenase. During paddle competition protein catabolism and muscle breakdown are activated, as are reflected in serum urea and creatine kinase levels. Although it seems to not be an important contribution of fatty acids to the source of energy an imbalance in electrolyte levels occurs, due to progressive water and electrolyte loss. This may be reflected in a state of fatigue. [ABSTRACT FROM AUTHOR]