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Signal detection plays an important role in the combination system of Radio over Fiber (ROF) link and MIMO-OFDM wireless channel. In this paper, a joint Maximum Likelihood (ML) and Minimum Mean Square Error (MMSE) estimation based signal detection, called as ML-MMSE, is firstly proposed for the MIMO-OFDM Radio over Fiber linear system. Then considering the increase of remote range and the use of nonlinear components in ROF link, we present a further improved scheme to overcome the nonlinear affect, which employs nonlinear channel estimation with fractional sampling technology. The simulation results demonstrate that the proposed detection method under the low SNR condition can improve the detection performance and control its system complexity at a lower level. [ABSTRACT FROM PUBLISHER]