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Immunostimulatory effect of Pirdot (Saurauia vulcani) has been reported previously. However, molecular interactions tend to be involved in mechanism of immunostimulatory activity has not been clarified. Our previous study indicated that ethanol extract of Pirdot leaves affected significantly the number of erythrocyte and spleen histological structure. Therefore, the purpose of the present study was to elucidate molecular bioactivity interaction of bioactive compound Pirdot with protein induced immunostimulant function through in silico approach. As a result, there were three bioactive compounds of Pirdot to interact with immunotherapeutic agents in protein-protein interaction pathway, such as ursolic acid, stigmasterol, and genistein. Moreover, its bioactive compound bound to active site of IL-8 and IL-6 that ursolic acid has the highest value than stigmasterol and genistein, respectively. [ABSTRACT FROM AUTHOR]

Objectives are to promote the Fagraea fragrans fruits as row materials of herbal cosmetics and to identify the secondary metabolites of these fruits before they are added to an cosmetic formulation as botanical ingredient for skincares. Key findings are to apply herbal cosmetic ingredient from Fagraea fragrans fruits with a number potent skin effects. They are 3.1 % of ursolic acid and its isomeric oleanolic acid and a number of the other compounds such as alkaloid, flavonoid, ginsenoside, and tannin-trimer as important bioactive cosmetic ingredient having beneficial for skin and other body parts. As summary, these fruits are rich with some botanical ingredients for cosmetics and they are also the potential plant in east coast of Sumatra Island, locally named buah tembesu. Therefore, the bioactive compounds of those fruits have to be mapped to consider their effects as well as their efficacy on skin. Fourteen compounds have been successfully identified with LCMS/MS from the filtrates of the methanol extracts of the fruits after the solid crystals of ursolic acid and oleanolic acid have completely precipitated as the major compounds. These fourteen compounds were also confirmed by comparing their molecular ion peaks to relevant compounds in some of references including the fragment ion peaks patterns and relevant botanicals as well. [ABSTRACT FROM AUTHOR]