1. Three-Dimensional Structure of Brain Tissue at Submicrometer Resolution.
- Author
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Rino Saiga, Ryuta Mizutani, Chie Inomoto, Susumu Takekoshi, NaoyaNakamura, Akio Tsuboi, Motoki Osawa, Makoto Arai, Kenichi Oshima, Masanari Itokawa, Kentaro Uesugi, Akihisa Takeuchi, Yasuko Terada, and Yoshio Suzuki
- Subjects
BRAIN physiology ,COMPUTED tomography ,MICROMETERS ,ZONE plates ,CELL physiology - Abstract
Biological objects are composed of submicrometer structures such as cells and organelles that are essential for their functions. Here, we report on three-dimensional X-ray visualization of cells and organelles at resolutions up to 100 nm by imaging microtomography (micro-CT) equipped with Fresnel zone plate optics. Human cerebral tissue, fruit fly cephalic ganglia, and Escherichia coli bacteria labeled with high atomic-number elements were embedded in epoxy resin and subjected to X-ray microtomography at the BL37XU and BL47XU beamlines of the SPring-8 synchrotron radiation facility. The obtained results indicated that soft tissue structures can be visualized with the imaging microtomography. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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