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The article offers information on the influences of protease and transglutaminase on the dyeing performance of wool with natural pigment sappan. The ultraviolet- (UV) visible spectrum analysis shows that the enzymes especially the transglutaminase makes the absorbance value of aqueous extract of sappan increase with some interaction between the enzymes and sappan dye. Meanwhile, untreated wool such as the transglutaminase has led to an increase in the color strength K/S value of wool dyed. Furthermore, the modification of protease has led to some decrease in wet rubbing fastness.