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Androgenesis represents one of the most important and fast-developing biotechnological approaches for the production of haploid and di-haploid crop plants with great potential for plant breeding. Tobacco serves as a suitable model plant with available protocols for androgenesis. However, it is necessary to understand the molecular regulatory mechanisms underlying this process. Phosphorylation is the most dynamic posttranslational modification. In a time, the cell contains only few percent of phosphoproteins that can furthermore be accompanied by their native forms. Thus, the enrichment techniques have to be applied in order to concentrate the phosphorylated forms. The aim of our studies was to compare the phosphoproteins extracted by TCA/acetone out of mature pollen and 30-min-activated pollen. The crude protein extracts were enriched by the metal-oxide/hydroxide affinity chromatography (MOAC) with aluminium hydroxide matrix. The efficiency of the enrichment was verified by 1D SDS-PAGE of the fractions coming out of the enrichment. The separated phosphoproteins were detected by phospho-specific ProQ Diamond stain (Invitrogen) whilst total proteins were stained by colloidal CBB G250. In this short paper, we present our aims in revealing the MOAC specificity for phosphoprotein enrichment. The enrichment of dephosphorylated mature pollen crude extract and the original mature pollen crude extract were compared. Since alkaline phosphatase did not accomplish the dephosphorylation step properly, we decided to apply cerium dioxide for sample dephosphorylation. Since cerium dioxide captured the phosphoproteins rather than dephosphorylated them, the proof of MOAC specificity was impossible to be achieved by these experiments. [ABSTRACT FROM PUBLISHER]

An abstract of the article "Potential role of the phosphoprotein PEA-15 in vascular disease" by G. F. Nixon is presented.

It is well recognized that the C-terminal domains of many proteins are important for directing them to heir correct cellular destination. Molecular genetic and cellular investigations of patients with a variety of inherited conditions that result from trafficking and targeting abnormalities have provided insights into the normal behaviour of membrane and other compartment residents. This talk will illustrate a range of such mechanisms that are of particular importance in the kidney, set in the context of a specialist multidisciplinary clinical service. [ABSTRACT FROM AUTHOR]