1. The Response of Antioxidant Genes in Rice (Oryza sativa L.) Seedling Cv. Cempo Ireng under Drought and Salinity Stresses.
- Author
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Refli and Purwestri, Yekti Asih
- Subjects
ANTIOXIDANT analysis ,DROUGHTS ,SALINITY & the environment ,RICE enzymes ,BIOSYNTHESIS - Abstract
The development of self-oxidative defense is a crucial mechanism of crops to survive in an unfavorable condition such as drought and salinity. The possible involvement of the antioxidant gene(s) in such defense under drought and salinity stresses was studied in leaves of rice (Oryza sativa L.) Cv Cempo Ireng seedling. Fifteen days old seedlings were subjected to 0.2 (extreme), 0.5 (mild) and 1.0 (control) FTSW (fraction of transpirable soil water as representative to drought stress) levels, and 0 mM (control) 125 mM (mild) and 250 mM (extreme) NaCl as representative to salinity stress levels for 6 d. The transcription levels of cCuZnSod
1 , cCuZnSod2 , MnSod1 , cApx1 , cApx2 , chl-tApx, Cat1 , Cat2 , Cat3 , Gr1 , Gr2 and Gr3 were analyzed using semi-quantitative RT-PCR technique. The antioxidant genes in seedling treated to drought showed a difference of transcription levels to seedling treated to salinity. The number of antioxidant genes increasing in transcription was lower in rice seedling under drought (cCuZnSod1 , cCuZnSod2 , cApx2 , cApx3 , Cat1 , Cat2 , Gr2 and Gr3 ) as compared to that in seedling under salinity (cCuZnSod1 , cCuZnSod2 , MnSod1 , cApx2 , cApx3 , Cat2 , Cat3 , Gr1 , Gr2 and Gr3 ). The present concluded that dismutation of superdioxide radical and biosynthesis of reduced ascorbic acid in gluthationine-ascorbate cycle within cells were less in seedling under drought stress so that the oxidative damage of seedling under drought was higher than that under salinity. [ABSTRACT FROM AUTHOR]- Published
- 2016
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