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In diabetes Mellitus, Achilles tendon revealed disorganized tendon fibers in 89% patients and calcification 76% patient. For this reason, diabetics patient prone to have tendon rupture. Stromal vascular fraction derived from adipose tissue contains adipose tissue-derived stem cells, which can differentiate into adipocyte, chondrocyte, osteoblast, myocyte, cardiomyocyte, and hepatocyte in vitro and also contains endothelial cells, smooth muscle cells, pericyte, fibroblast, mast cells, and pre-adipocyte cells. The stromal vascular fraction can be alternative treatment as a source of progenitor cells on tendon healing in the diabetic patient. Eighteen adult male rat (Rattus norvegicus strain Wistar), 150-200 mg weight were divided into 3 groups. The other five rats were used as donors of adipose tissue. Group 1 was normoglycemia, group 2 diabetes Mellitus and group 3 diabetes Mellitus injected with the stromal vascular fraction. Each rat was performed Achilles tenotomy, tendon repair and immobilized with a cast for two weeks. Animals were sacrificed at the end of the second week and tendons underwent a histological evaluation to measure fibroblast and capiler. Amount of collagen measured with a colorimeter. Stromal vascular fraction increased the number of fibroblast, angiogenesis and amount of collagen in rat achilles tendon with diabetes significantly compared to group 1 normoglycemia and group 2 diabetes mellitus (p<0,05). The stromal vascular fraction from adipose tissue increased the number of fibroblast, angiogenesis and the amount of collagen in rat’s achilles tendon with diabetic mellitus. Thus, stromal vascular fraction improved healing on tendon rupture in diabetes mellitus. [ABSTRACT FROM AUTHOR]

Growing cells in plastic with liquid media for in vitro study is very common but far from physiological. The use of scaffold materials is more biocompatible. Extracellular matrix provides tissue integrity which acts as a native scaffold for cell attachment and interaction, as well as it serves as a reservoir for growth factors. For this reason, we have developed natural scaffold from mice fibroblast to form a natural scaffold for stem cell culture. Fibroblasts were cultured under crowded condition and lysed to form natural scaffold. The natural scaffold formation was observed using immunofluorescence which then will be used and tested for stem cell propagation and differentiation. [ABSTRACT FROM AUTHOR]

Growing cells in plastic with liquid media for in vitro study is very common but far from physiological. The use of scaffold materials is more biocompatible. Extracellular matrix provides tissue integrity which acts as a native scaffold for cell attachment and interaction, as well as it serves as a reservoir for growth factors. For this reason, we have developed natural scaffold from mice fibroblast to form a natural scaffold for stem cell culture. Fibroblasts were cultured under crowded condition and lysed to form natural scaffold. The natural scaffold formation was observed using immunofluorescence which then will be used and tested for stem cell propagation and differentiation. [ABSTRACT FROM AUTHOR]

This paper presents a preliminary work toward the understanding of cell-material interaction on the surface of biodegradable metals. It is interesting to study this interaction considering the active surface of biodegradable metals that is subjected to degradation process. Pure porous iron and biodegradable polymers-infiltrated porous iron samples were investigated for its ability to promote cell adhesion and growth using fibroblast cells. The cellular morphology and behaviour of the cells on the surface were observed after 24 hours using microscopic techniques. The results of cytotoxicity test revealed that the metals were non-cytotoxic. Under optical microscope, the cells were quite sparsely distributed, and few cell-to-cell contacts were observed. Some cells spread thinly with the irregular surfaces wall of porous iron. It was also shown that cells adhered preferably on the surface of the pure porous iron compared to that of polymers-infiltrated one. [ABSTRACT FROM PUBLISHER]