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The level of antibodies to fasciolas in the milk of cows from the Moscow region of Russia was assessed. In a dairy farm in the Moscow region of Russia, a milk sample was taken and the amount of antibodies to Fasciola hepatica (ODRf) and Ostertagia ostertagi (ODRo) was estimated in it. Their level was processed by a multivariate linear regression model with the elucidation of their relationship with the level of milk protein, % milk fat and the interval between past calving. The increase in ODRf turned out to be associated with a decrease in the level of average milk yield per year with a decrease in the average level of milk fat content with an increase in the average time interval between departments in the absence of a significant relationship with the level of protein in milk. The relationship of ODRf and ODRo with milk yield showed an additional, rather than synergistic effect of infection with both parasites at once. [ABSTRACT FROM AUTHOR]

Fasciola hepatica, also known as the common liver fluke or sheep liver fluke, is a parasitic trematode of the class Trematoda, phylum Platyhelminthes that infects the livers of various mammals, including humans. The disease caused by the fluke is called fascioliasis, which is a type of helminthiasis and has been classified as a neglected tropical disease. Catalase (EC 1.11.1.6) present in the peroxisomes nearly all aerobic cells, serves to protect the cell from the toxic effects of hydrogen peroxide by catalysing its decomposition into molecular oxygen and water without the production of free radicals. In the present study, we aimed to investigate the role of antioxidant enzymes against toxic reactive oxygen species in human fascioliasis. This study was conducted on patients with fascioliasis and compared with healthy controls. Fasciola hepatica was determined by microscopy and antibody detection (Indirect haemagglutination test) analysis. We assayed catalase levels measured of 24 subjects and matched in 36 healthy controls. The catalase activity was measured by Luck. The difference between catalase levels of patients (13,86x104±2,07x104 IU/mg hemoglobin) was lower than control groups (17,23x104±1,87x104 IU/mg hemoglobin) and was statistically significant (p<0.05). In conclusion, there is an increase in oxidative stress in a fascioliasis. Despite this stress, the antioxidant system is deficient and adequate, in patients with fascioliasis. [ABSTRACT FROM AUTHOR]