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Induction and synchronization of estrus during non-breeding season is the most frequently used biotechnological method of assisted reproduction in sheep. Synchronization of estrus in sheep is a very important breeding technique as it can effectively improve the reproductive performance of sheep, minimize the effects of seasonal factors on sheep reproduction, extend the breeding season of sheep, shorten the estrus interval, increase the gestation rate, increase the birth rate, it can also lead to the shortening of the time between generations. The study proposes a method of inducing and synchronizing estrus during non-breeding season in Turcan sheep, which uses vaginal sponges with FGA (20 mg), supplemented by the administration of PGF2a (0.20 mg) and GnRH (7 µg). The analyzed parameters were: sponge loss rate, vaginitis onset rate, total percentage of ewes in estrus and gestation rate. The results obtained for the induction rate and synchronization of estrus during non-breeding, the reproductive period in sheep from the Turcana breed of 88.88%, respectively for the rate of pregnancy deception of 81.25% indicate that the method used was an efficient one. [ABSTRACT FROM AUTHOR]

The objective of this study was to determine the concentration of progesterone in Aceh cows. A total of 10 Aceh cows aged 3 - 7 years, weighing 150 - 250 kg, clinically healthy, and having normal reproduction (have been breeding and have experienced at least two regular cycles) were used in this study. Estrus synchronization was carried out in all cows using the PGF2α and then Artificial Insemination (AI) was performed during estrus. Serum sample collection was carried out on days 5th, 6th, and 7th after AI to examine progesterone concentrations. The concentration of progesterone was measured using the enzyme-linked immunosorbent assay (ELISA) technique. Pregnancy and embryonic death were examined by ultrasonography on days 25 to 45 post AI at 10-day intervals. The average concentration of the progesterone in pregnant Aceh cows and cows that experienced early embryonic death on days 5, 6, and 7 after AI were 2.75 ± 3.21 and 0.71 ± 0.32; 3.37 ± 2.89 and 0.96 ± 0.71; 8.02 ± 5.19 and 1.61 ± 0.77 ng/mL, respectively. In conclusion, the concentration of progesterone in pregnant Aceh cows tends to increase and higher than Aceh cows with early embryonic death. [ABSTRACT FROM AUTHOR]