Search

Plasma is the fourth state of matter, which is the ionic state of matter and is composed of negative electrons, positive ions, and neutral particles, Plasma is classified according to its temperature into high-temperature plasma and low-temperature plasma which is at room temperature (≈ 300 K), Low-temperature plasma has wide applications: medical, environmental, industrial and surface treatment, in our research, the DBD system was used to produce cold plasma for using in studying its effecton Poly-methyl Methacrylate (PMMA) polymer. Four polymer samples were made. One of them was left as a control one, and theremaining three were exposed to plasma at different time intervals: 10, 20, and 30 minutes. the effect of plasma on the chemical composition of the polymer surface was studied using a Fourier Transform Infrared (FTIR) spectrophotometer. the tests showed a the carbonyl group peak shifted from 1744 cm−1 to a new position at 1720 cm−1 and changed its amplitude (>C=O) stretching vibrations, and the peak shifted from 735 cm−1 to a new position at 744 were attributed to C-C skeletal mode, Whereas the examinations of the atomic force microscopy (AFM), was shown that the polymer plasma treatment leads to irregular changes in the surface roughness properties of the samples Pure-PMMA was modified by plasma using DBD at different treated times ranging from (10 to 30 minutes), and the results show that the treatment times enhance surface roughness and crystallinity, but after extensive treatment, the value of surface roughness dramatically decreased, and a show the Hardness is decreased with increasing exposure to plasma at (10, 20, 30) min, the PMMA becomes softer after being treated to the plasma. [ABSTRACT FROM AUTHOR]

In the present study two breast cancer cell lines (MCF7and AMJ13) with another normal (ASCs) were treated indirectly with cold atmospheric plasma (plasma activated media representing PAM) which were incubated for 24 and 48 hrs. Physical parameters of plasma jet were determined by optical emission spectroscopy, electron temperature, and electron density detected by the Boltzmann and stark broadening equations for different gap distances (0.5,1, and 1.5 cm) with constant gas flow of 3L/minute. The present study was focused on their effect on breast cancer antioxidant enzyme (TSOD) and tumor derived-IgG. First, the effect of PAM on total super oxide dismutase (TSOD) enzyme activity was evaluated and the results demonstrated a significant elevation in MCF7after 24 hrs. of incubation (65.85± 2.5 and 84.2 ±3, P˂0.05) and significant decline after 48 hrs (62 ±1.4 and 80.95± 1.3, p˂0.01) for treated and untreated cells respectively. While in AMJ13significant results was seen only after 48 hrs. (69.67±0.75 for treated and 83.2±3untreated cells; p˂0.05), ASCs data was conflicted with significant increase found only after 48 hrs (p˂0.05) (87.1±2 for treated and 69.3±1fo control). Tumor derived-IgG results was significantly altered in MCF7 cells (40.4± 0.49, 31.1± 0.5; p=0.037) ng/ml after 24 hrs and (43.4± 0.02 and 36.3± 0; p=0.045) ng/ml after 48 hrs. for treated and untreated cells. AMJ13 showed significant results only after 48 hrs of incubation (23.19± 3.74 vs. 13.95± 3.74; p=0.030) ng/ml, ASCs showed significant alteration only at 48hrs with an increase in the level of IgG. [ABSTRACT FROM AUTHOR]