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Blot e-Learning is bio-computing approach to design an effective e-Learning system. E-Learning practice has achieved an enormous momentum, it makes a tremendous impact in education and corporate, each year more of the world's population becomes connected into internet. By the last decade many virtual universities and online-educational sectors emerged, but people were not much benefitted by e-learning even though it is cheaper. It is why because it had no effective impact on people. The common online e-Learning [2] materials may not meet the all requirements of people. The universal truth is no human being are the same, based on our research apart from the environment and nature each human Blood Group (B.G) plays main role in human intelligence, behavior, interest & learning capacity. We analyze similarities and differences of human by their blood types. We generated and designed an effective e-Learning system named Blot (Biology) e-Learning system. It discussed and despite the universal standard and ample support to the successful e-learning and e-teaching system to be instant implementation. The Blot e-Learning system concentrates on high quality in education sectors, better productivity in corporate finally, to make e-Learning is more comfort. [ABSTRACT FROM PUBLISHER]