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This paper focuses on the evaluation of the level of ecological stability of the landscape and on the degree of anthropogenic impact on the landscape in a regular square of grid (real size squares 0.25 km²) in an ArcView GIS 3.2. Model area represents the cadastral territory of the Bardejov city (72.8 km²), which is located in the northern part of the Eastern Slovakia. First method which we have used in this contribution is based on the evaluation the meaning of the land cover classes in the meaning the work of [11]. The second method was based on the exploring the variation the coefficient of ecological stability of the landscape within the relations of the anthropogenic interference into the landscape structure [8]. The underlays for monitoring the intensity of anthropogenic intervention of, as well as the degree of eco-stabilizing ability of the country have become the layers of a secondary landscape structure (land cover classes). Since that the anthropogenic interference in landscape may cause irreversible changes in the landscape structure, these changes were recorded in the time-spatial context (time horizons 1985, 2009). The importance of the work is in the comparison of two methods of research and its results. The importance of the work is in the comparison of two methods of research and its results with a focus on the fluctuation the index of ecological stability in the square of grid with minimal surfaces. Represents possibility efficiency of research based on the use of homogenous spatial units. [ABSTRACT FROM AUTHOR]