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Temperature and humidity chambers are widely used for testing of a wide range of biological items, materials, electronic devices, and components and calibration of temperature and humidity sensors, thermohygrometers and other humidity devices. It is crucial to verify the chamber's performance regularly to ensure that its environmental condition complies with the target specifications. The Standards and Calibration Laboratory (SCL) developed a technical procedure for measuring the performance of a temperature and humidity chamber, referencing IEC 60068-3-5:2018 and IEC 60068-3-6:2018 standards. A multi-channel temperature logger with platinum resistance thermometer sensors was used for temperature measurement, while a dew-point hygrometer was used as humidity measuring sensor for relative humidity measurement. The method for temperature measurement had been discussed in a previous publication [5], and the method for relative humidity measurement were discussed in this paper. A detailed procedure for equipment setup and measuring the achieved humidity, humidity fluctuation, humidity gradient, and humidity variation in space are provided. Examples of measurement results together with the uncertainty evaluation are presented. [ABSTRACT FROM AUTHOR]