1. Potential of waste starch substrate as a bioplastics production substrate by microbes.
- Author
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Hafis, Putri, Rahmau Udita Surya Aradea, Ahmad, La Ode, and Susilowati, Prima Endang
- Subjects
CASSAVA starch ,BACTERIAL colonies ,BIODEGRADABLE plastics ,CORNSTARCH ,MICROORGANISMS ,BACTERIAL cells ,CELLULOSE ,STARCH - Abstract
Bioplastics are plastics that microorganisms can degrade from compounds in plants such as starch, cellulose, and lignin. The study determines "Potential of Starch Substrate from Waste as a substrate for Bioplastic Production by Microbe". Qualitative determination of bioplastic accumulation was carried out by Sudan black painting. Painting was carried out on bacterial colonies in minimally solid media at a temperature 30°C in 48 hours. Colonies bacterial that grew then dissolved in ethanol and grown in media liquid minimally, a source of starch carbon from the waste. Use a shaker speed in 125 rpm and temperature of 30 °C for 72 hours. Cells bacterial were extracted by centrifugation for 20 minutes at 3000 rpm. Determined content of the bioplastic was using a UV-Vis spectrophotometer at wavelength of 235 nm with concentrated H
2 SO4 as solvent. The research conducted by screening indigenous bacteria for bioplastic production showed that from 24 bacterial isolates, 13 isolates produced bioplastic using peel starch cassava, 5 isolates produced bioplastic using cassava rice-washed, and there were no bacterial isolates using peel starch banana. The results of the screening of 13 isolates showed that the bacterial isolate with code S110-5A1 had an absorbance value of 3.6989, and the results of the screening of 5 isolates showed that the bacterial isolate with the code S110-3A2 had an absorbance value of 1.5654, indicated which the potential producer of bioplastics good. [ABSTRACT FROM AUTHOR]- Published
- 2023
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