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This paper presents an analysis of trends in drought variables at 29 stations across the Euphrates-Tigris Basin over the period 1965–2022. Drought variables were calculated using the Standardized Precipitation Index (SPI) for 1-, 3-, 6- and 12-month scales. The drought values have been decomposed for these time scales through application of Discrete Wavelet Transform (DWT). The components are defined as short, medium and long periodicities. DWT-based trend analysis was computed using Mann-Kendall (MK) and Innovative Trend Analysis (ITA) methods. As the time scale and the periodicity of drought increase, a strong increase in drought occurs. It was revealed that drought increased significantly at all time scales in almost all stations, especially in the long periodicity, according to both MK and ITA. The droughts and the serious increasing trend of droughts in this basin constitute a significant threat to water resources. • Drought in the largest basin of Turkey, the Euphrates-Tigris Basin, was scrutinized through an assessment of the Standard Precipitation Index (SPI). • Short, medium, and long periodicities acquired by decomposing Standardized Precipitation Index (SPI) time series have been introduced. • The trends of SPI time series and short, medium and long periodicity series were analysed by MK and ITA tests. • The most severe droughts began after 2020 throughout the basin and the drought intensified significantly during the long periodicity. [ABSTRACT FROM AUTHOR]