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A serum-free transient expression method for antibodies in HEK cells and a standardised purification protocol were developed. The methods described enable the fast and cost-effective production of hundreds of milligrams of different antibodies that can be used for test purposes. The N-glycan profile of the antibodies obtained is comparable to that of antibodies expressed in stable CHO cell lines, confirming the suitability of the system to produce material for target validation studies. [ABSTRACT FROM AUTHOR]

The described repeated batch mode combines the advantages of fed batch and perfusion processes. Harvesting is performed in preset intervals by a continuous-flow centrifuge (Viafuge from Carr, Pneumatic Scale INC:). The cells can be returned into the fermenter without loss of viability. The harvested medium is replaced by pre-heated fresh medium and the cell concentration for re-inoculation can be readjusted to the requested level [ABSTRACT FROM AUTHOR]