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Your search keyword '"Schepetkin, Igor A."' showing total 5 results
Start Over Author "Schepetkin, Igor A." Publication Type Books
5 results on '"Schepetkin, Igor A."'

1. Isolation of Neutrophils from Nonhuman Species

Author

Siemsen, Daniel W., primary, Kirpotina, Liliya N., additional, Malachowa, Natalia, additional, Schepetkin, Igor A., additional, Porter, Adeline R., additional, Lei, Benfang, additional, DeLeo, Frank R., additional, and Quinn, Mark T., additional

Published
2019
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2. Neutrophil Isolation from Nonhuman Species

Author

Siemsen, Daniel W., primary, Malachowa, Natalia, additional, Schepetkin, Igor A., additional, Whitney, Adeline R., additional, Kirpotina, Liliya N., additional, Lei, Benfang, additional, DeLeo, Frank R., additional, and Quinn, Mark T., additional

Published
2014
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5. Neutrophil Isolation From Nonhuman Species.

Author

Walker, John M., DeLeo, Frank R., Bokoch, Gary M., Siemsen, Daniel W., Schepetkin, Igor A., Kirpotina, Liliya N., Lei, Benfang, and Quinn, Mark T.

Abstract

Advances in the understanding of neutrophil biochemistry require the development of effective procedures for isolating purified neutrophil populations. Although methods for human neutrophil isolation are now standard, similar procedures for isolating neutrophils from many of the nonhuman species used to model human diseases are not as well developed. Because neutrophils are reactive cells, the method of isolation is extremely important to avoid isolation technique-induced alterations in cell function. We present methods here for reproducibly isolating highly-purified neutrophils from large (cow, horse, sheep) and small (mouse, rabbit) animal models and describe optimized details for obtaining the highest cell purity, yield, and viability. We also describe methods to verify phagocytic capacity in the purified cell populations using a flow cytometry-based phagocytosis assay. [ABSTRACT FROM AUTHOR]

Published
2007
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