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Having many characteristics of an ideal experimental system, Arabidopsis thaliana became a very important model system for flowering plants. Its completed genome sequence data provided scientists the first fundamental tool towards understanding its genome structure and genes that it possess. There are more than 33,000 predicted genes in Arabidopsis and this number is increasing as novel methods develop and our understanding of genome organization and regulation expands. Reverse genetics that aim to reveal the functions of all Arabidopsis genes and the related resources were next most important tools that plant scientists needed. Numerous consortia were formed to supply scientists with such resources and tools necessary to determine the functions of Arabidopsis genes. Thanks to these international community efforts, now there are around 426,000 independent T-DNA/transposon insertion lines representing near saturation of all genes in Arabidopsis available for the research community. Besides insertion lines, several other sophisticated technologies and resources crucial for large-scale gene function studies in Arabidopsis were also developed. In this chapter, we discuss most of these important reverse genetics resources for gene function analysis. [ABSTRACT FROM AUTHOR]