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Your search keyword '"A. Sachinidis"' showing total 18 results
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18 results on '"A. Sachinidis"'

1. Next generation sequencing in toxicology

Author

Vavilis, Theofanis, primary, Stamoula, Eleni, additional, Sachinidis, Athanasios, additional, Lamprinou, Malamatenia, additional, Dardalas, Ioannis, additional, Ainatzoglou, Alexandra, additional, Drakoulis, Nikolaos, additional, and Anagnostopoulos, Athanasios K., additional

Published
2023
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2. Nutraceuticals and Cardiovascular Disease

Author

Chianetta, Roberta, primary, Sachinidis, Alexandros, additional, Nikolic, Dragana, additional, Luzzu, Luca Marco, additional, Stoian, Anca Pantea, additional, Toth, Peter P., additional, and Rizzo, Manfredi, additional

Published
2021
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8. Embryonic Stem Cells and Their Therapeutic Potential

Author

Šarić, Tomo, primary, Chen, Shuhua, additional, Kamisetti, Naidu, additional, Halbach, Marcel, additional, Doss, Michael Xavier, additional, Winkler, Johannes, additional, Hescheler, Jürgen, additional, and Sachinidis, Agapios, additional

Published
2008
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10. The Oligomerization of Dilute Ethylene Streams

Author

Brown, Stephen J, primary, Clutterbuck, Linda M, additional, Conyngham, Michael J, additional, Hinton, Cassandra, additional, Humphreys, Geoffrey B, additional, Masters, Anthony F, additional, Sachinidis, John I, additional, Tregloan, Peter A, additional, and Wasiowych, Christine D, additional

Published
1994
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12. Omics and related recent technologies

Author

Ainatzoglou, Alexandra, Vavilis, Theofanis, Stamoula, Eleni, Dardalas, Ioannis, Sachinidis, Athanasios, Lamprinou, Malamatenia, Drakoulis, Nikolaos, and Anagnostpoulos, Athanasios K.

Published
2015
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15. Culture of Embryoid Bodies

Author

Gissel, Cornelia, primary, Nierhoff, Dirk, additional, Fleischmann, Bernd, additional, Hescheler, Jürgen, additional, and Sachinidis, Agapios, additional

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18. Generation and Characterization of Cardiomyocytes Under Serum-Free Conditions.

Author

Walker, John M., Turksen, Kursad, Gissel, Cornelia, Doss, Michael Xavier, Hippler-Altenburg, Rita, Hescheler, Jürgen, and Sachinidis, Agapios

Abstract

In vitro culturing of mammalian cells provides an elegant platform to study cell signaling, interactions, and metabolism as well as proliferation and differentiation processes. Often, these cells are cultured and maintained in sera obtained from animals such as horses, cows, and rabbits. The sera used for this purpose fluctuates in composition from individual animals and, hence, influences the cellular growth and differentiation at different magnitudes. This poses a need to use a substitute for sera in cell culture systems to overcome the observed variations. Here, we present and compare protocols for culturing of embryonic stem (ES) cells in serum-free conditions, derivation of germ layers, and cardiac differentiation of ES cells in both serum-free and serum-containing culture conditions. Differentiated embryoid bodies by serum-free protocols produce significantly increased frequencies of clusters of cardiac cells beating stronger than found in serum-containing media. Therefore, we conclude that the use of serum replacement media (SRM) in our experiments led to more specific differentiation but reduced proliferation because these SRMs contained reduced essential substances like growth factors and hormones. Unlike serum media, SRMs have a welldefined composition and are highly reproducible. Hence, SRM will be the ideal substitute for serum-containing media. [ABSTRACT FROM AUTHOR]

Published
2006
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