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Abstract The Tianshanbeilu cemetery is the largest Bronze Age cemetery in eastern Xinjiang, China, and plays important roles in connecting the Eurasian interior to the Hexi Corridor, and further to the Central Plains region. Utilizing micro-XRF and Raman spectroscopy techniques, we identified a fluorite bead at this cemetery. This barrel bead is the earliest record of such a fluorite bead in China, dating back to approximately 1385–1256 BCE. Comparing the unearthed records of fluorite beads in eastern China spanning from the Neolithic Age to the Western Zhou dynasty, we notice that in the early Western Zhou period, fluorite beads found in élite burials are only in barrel or biconical shapes, both of which first appeared in the eastern region of Xinjiang, such as the Tianshanbeilu cemetery in Hami and the Yanghai cemetery in the Turpan Basin. We proposed that the barrel fluorite bead drilled by metal tubular drill first appearing in the Tianshanbeilu cemetery might have spread eastward to the Central Plains region and finally constituted a component of the ritual revolutions during the Western Zhou dynasty.

Abstract The process of staining was frequently employed to enhance or alter the color of agate beads in ancient times. One of the key challenges in studying ancient beads is comprehending the intricate techniques employed to color agate stones. An understanding of the staining mechanism from a mineralogical standpoint offers insights into the level of technological advancement in different civilizations. In this study, the mineral structure of eight ancient agate beads from Xinjiang Uygur Autonomous Region, NW China, was analyzed using Micro X-ray fluorescence (µXRF), Raman spectroscopy, Scanning Electron Microscope (SEM), and Fourier Transform Infrared (FTIR) techniques. The color, transparency, mineral phase, and surface roughness of the beads were examined, revealing variations ranging from colorless to light violet to dark violet. Raman and FTIR spectroscopy were employed to determine the SiO2 phase and the changes in optical characteristics of agate beads after artificial staining. The black color of the beads was formed by carbon penetration, while the red color was produced by heating. The coexistence of α-quartz and moganite phases in the red, the dark red, the black, the idiochromatic white and the part translucent zones of the ancient beads was confirmed by the 464 cm−1 peak of α-quartz and the 502 cm−1 peak of moganite phase. The analyzed red, the dark red, the black, the idiochromatic white and the part translucent zones exhibited remarkably similar FTIR spectral features, with two prominent bands at ~ 1097 and ~ 1187 cm−1, as well as two weak bands at 798 and 778 cm−1, indicating the presence of moganite and α-quartz in the unetched ancient beads. In contrast to the idiochromatic white appearance of natural agate, the scattered white coloration in etched beads was generated by an etching reaction. Both Raman and FTIR spectroscopy indicated the absence of moganite in etched beads, indicating that the scattered white color was produced by the loss of moganite and a portion of α-quartz, resulting in a rough surface.

Bioavailable strontium isotope analysis was proposed for prehistoric human ecology almost 40 years ago and rapidly became one of the most important tools to trace past migratory behaviours. Since its first introduction to China in 2003, this method has greatly improved our understanding of migrations on individual- and community-scales over the past 20 years. This paper summarizes the current state of knowledge regarding identifying non-locals, utilizing animal resources, and strontium isoscapes for China based on empirical data. By incorporating additional baseline data, we updated and extended the current bioavailable strontium isoscape for China and Southeast and South Asia. These data will shed new light on intercultural communications and the spread of customs and technologies. In the future, strontium isotope analysis will involve an integrated provenancing system along with multiple approaches such as various isotopes and different modellings. Correspondingly, the existing isoscape needs to improve its spatial resolution and predictive accuracy to source the non-local archaeological biological remains. Furthermore, advances in analytical techniques allow us to reconstruct lifetime mobility of animals and humans at high temporal resolution.

A newly excavated ritual site, Xuechi, exposed abundant sacrificial livestock, allowing to understand the supplies and managements of livestock during the early Chinese Qin-Han Empires. Here we reported animal enamel 87Sr/86Sr values from the Xuechi site that are obviously higher than those of local snails and surface water, as well as calcites of the Chinese Loess Plateau (CLP) where the site was located, strongly suggesting that these sacrificial animals were not raised in the vicinity of the site. The regional bioavailable 87Sr/86Sr isoscape further indicates that some livestock were probably derived more than one hundred kilometers away, suggesting a complex social network for recruiting and managing the sacrificial activities. In the context of other Neolithic-Bronze Age records on the CLP, an increasing trend in variation of faunal 87Sr/86Sr values shows a robust correlation to Chinese states’ trajectory, in concordance with the territorial expansion model for state formation.

In order to alleviate problems such as large fluctuations in grid load caused by the high proportion of renewable energy, circulating fluidized bed (CFB) power plants undertake the task of rated load regulation. This study discussed the combustion characteristics of a 100 KW CFB boiler during the operation process of varying loads and analyzes the combustion characteristics, load regulation rate and emissions variation law during the operation process of high- and low-rated load intervals. The experimental results showed that under the condition of a high-rated load, the average temperature of each area in the furnace was proportional to the size of the load. Under low-rated load conditions, the temperature change increased first and then decreased with the reduction in load. In the 30% load stage, the lowest temperature in the riser was 740 °C, while the temperature in the loop seal was even as low as 650 °C. The concentrations of O2, CO and unburned carbon mainly depended on the combustion reaction intensity under each load condition, which showed a higher trend at low load (30%). In terms of NOx emission, it was proportional to the load in the high-rated load range. However, the NOx generation at the 30% load was about 30 mg/Nm3(@6%O2) higher than the 50% load. In addition, the regulation load rate (2.5%/min) between high-load conditions exhibited significantly greater than that between low-load conditions (0.78%/min). Therefore, the low-load operation will face problems such as low furnace temperature, uneven gas–solid fluidization, and difficult control of pollutant generation, which need to be paid attention to during operation.

Many questions still remain regarding the acquisition and circulation of ancient domesticated animals across the Yellow River Basin, one of the key areas for the development of complex societies in ancient China. Here, we re-evaluate previously published strontium isotope data (87Sr/86Sr, n = 167) from tooth enamel of domesticated animals at 10 archaeological sites in the Yellow River Basin to shed new light on the transition between the Neolithic (7000–5000 BCE) and the Western Zhou Dynasty (1046–771 BCE). The results show that from the Late Neolithic to the Western Zhou Dynasty, some domesticated animals, mostly cattle and sheep, were increasingly sourced from non-local areas. We employed Bayesian methods to define an isoscape of bioavailable Sr for the Yellow River Basin and to show the considerable diversity in the origins of non-local domesticated animals, some of which may have come from locations hundreds of kilometers away from the site as early as the Late Neolithic. The increasingly variable 87Sr/86Sr ratios of domesticated animals from the Neolithic to the Western Zhou Dynasty are consistent with that of associated human remains, and also match the archaeological and zooarchaeological evidence for increased circulation of animal products in the Yellow River Basin. Therefore, we infer that local economies increasingly incorporated non-local animals as part of wider circulation networks that emerged with the development of complex societies since the Late Neolithic.

The Notch signaling pathway plays an important role in otic neurogenesis by regulating the differentiation of inner ear hair cells and supporting cells. Notch-regulated differentiation is required for the regeneration of hair cells in the inner ear. The temporal expression pattern of Notch ligands and receptors during in vitro hair cell-like cell differentiation from human embryonic stem cells (hESCs) was detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Subsequently, pAJ-U6-shRNA-CMV-Puro/GFP recombinant lentiviral vectors encoding short hairpin RNAs were used to silence JAG-1, JAG-2, and DLL-1, according to the temporal expression pattern of Notch ligands. Then, the effect of each ligand on the in vitro differentiation of hair cells was examined by RT-PCR, immunofluorescence, and scanning electron microscopy (SEM). The results showed that the individual deletion of JAG-2 or DLL-1 had no significant effect on the differentiation of hair cell-like cells. However, the simultaneous inhibition of both DLL-1 and JAG-2 increased the number of hair cell-like cells and decreased the number of supporting cells. JAG-2 and DLL-1 may have a synergistic role in in vitro hair cell differentiation.

In this paper, we developed a two-step-induction method of generating functional hair cells from inner ear multipotent cells. Multipotent cells from the inner ear were established and induced initially into progenitor cells committed to the inner ear cell lineage on the poly-L-lysine substratum. Subsequently, the committed progenitor cells were cultured on the mitotically inactivated chicken utricle stromal cells and induced into hair-cell-like cells containing characteristic stereocilia bundles. The hair-cell-like cells exhibited rapid permeation of FM1-43FX. The whole-cell patch-clamp technique was used to measure the membrane currents of cells differentiated for 7 days on chicken utricle stromal cells and analyze the biophysical properties of the hair-cell-like cells by recording membrane properties of cells. The results suggested that the hair-cell-like cells derived from inner ear multipotent cells were functional following differentiation in an enabling environment.

Located on the Pamir Plateau in Xinjiang Province, China, the Ji'erzankale Necropolis dates back to 2500 yr BP. Many materials that have been unearthed in this cemetery, including shoo konghou (musical instrument), bronze mirrors and glass beads, suggest cultural transference between East and West. Furthermore, small-sized and rounded fire altars made from sweet-scented Sabina were found for the first time and regarded as implements for fire veneration. We identified 70 wooden objects from 25 tombs within the Necropolis, and found that each object had been made from one of seven tree species. Analysis revealed that the inhabitants of the region mainly used the most widely available types of wood, namely Betula and Populus. People also specifically chose inflammable Populus wood to make hearth boards and hand drills (both are used for making fire by drilling), rigid Betula wood to craft wooden plates. Salix was used for fashioning wooden sticks, while sweet-scented Sabina was the preferred choice for making fire altars. Lonicera was selected for arrow shaft manufacture and Fraxinus syriaca, which has a beautiful grain, was chosen for making musical instruments. Conscious selection of different types of wood indicates that people of the Pamir Plateau were aware of the properties of various types of timbers, and were able to exploit these properties to the full. In turn, this demonstrates their wisdom and their ability to survive in, and adapt to, their local environment.