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The heart valve design is optimally adapted to their physiological function, i.e., the uniform distribution of blood flow in the heart chambers, so even small changes in the structural components of the valves, which are initially functionally compensated, play a significant role in the development of heart disease in the later stages of a patient's life. The study aimed to determine the effect of chronic haemodynamic trauma on the structural reorganisation of human heart valves. A total of 1377 human heart valves were evaluated, which were obtained during valve prosthetics at the Amosov National Institute of Cardiovascular Surgery from 2010 to 2022. The study identified a full range of macro- and microscopic changes in morphological structures that are characteristic of rheumatic heart disease, infective endocarditis, dysplastic and destructive changes in heart valves. The set of all morphological features was combined into the algorithm “Morphological manifestations of acquired heart disease of different genesis”. It has been established that changes in the mitral valve architecture can cause the transformation of its structural components, which causes a violation of the valve's functional capabilities. The study demonstrated that persistent changes associated with chronic haemodynamic injury reduce the mechanical strength of the valve, despite the compensatory mechanism of subendothelial smooth muscle fibre hyperplasia in the heart valve leaflets. Subsequently, these damages can, on the one hand, be complicated by fibrosis, fatty degeneration and calcification, and on the other hand, compensatory mechanisms are involved in the process, namely hyperplasia of subendothelial muscle cells, which often form continuous layers that should strengthen and strengthen the mechanical strength and thus the functional competence of the valves. Mitral valve dysplasia can develop secondary infective endocarditis, rheumatism, and degenerative valve disease. The algorithm for determining the patterns of morphological changes in the valvular apparatus of the heart in various variants of acquired defects of inflammatory and non-inflammatory genesis can be used to improve the etiopathogenetic drug treatment of patients with acquired heart disease, as well as a theoretical basis for the development of new types of valve-preserving operations

У більшості випадків очищення вихідного продукту процесу виробництва оцтової кислоти здійснюється шляхом ректифікації. Оскільки процес ректифікації полягає в розділенні сумішей на фази по температурі кипіння, то стає зрозуміло, що досить важливим технологічним параметром є температура та концентрація суміші, що обробляється. Зазвичай, дані технологічні параметри задаються кип’ятильником при ректифікаційній колоні, який ще зветься кубом колони. Саме тому існує потреба у якісному та ефективному керуванні кип’ятильником, як одним із важливих технологічних об’єктів. У роботі розроблена та проаналізована система керування кип’ятильником на базі MPC-регулятора. Робота MPC-регулятора базується на визначенні поведінки об’єкта та прогнозування майбутньої поведінки, після чого за рахунок оптимізації керування на певному проміжку часу здійснюється виведення його на бажану траєкторію. Проміжок часу на якому здійснюється розрахунок оптимальної траєкторії ще називається горизонтом. Визначення горизонту впливає на характер здійснюваного керування, тому його вибір повинен бути обраний виходячи з характеру динамічних властивостей об’єкту керування. Саме тому при розробці даних регуляторів потрібно досліджувати характер впливу даних параметрів на перебіг процесу. В результаті дослідження було отримано стратегії керування для різних значень горизонтів прогнозування та керування. Проаналізувавши результати роботи регулятора при різних налаштуваннях горизонтів прогнозування та керування можна дійти висновку, що при збільшенні горизонту прогнозування збільшується час перехідної характеристика, але також зменшується перерегулювання. Тому при виборі оптимального значення потрібно керуватися впливом перерегулювань на процес.

The purpose of this study is to ground the causality, its character, and power between economic growth and housing spending within government social protection to strengthen poverty reduction. The study is conducted on a sample of 27 EU countries for 2012–2021 according to the following indicators: government expenditure on housing within social protection, government expenditure on housing development, and GDP per capita growth. Correlation analysis revealed the relationship between the variables. The paper employed time lags with the most significance based on Pearson and Spearman correlation coefficients depending on normal data (Shapiro-Wilk test). The causal analysis determined which of the studied indicators is the cause and consequence of established dependence based on the results of the Granger test. Calculations are made in STATA software. It is confirmed that government expenditure on housing within social protection influences GDP per capita growth in 14 countries (the highest impact is in Greece and Spain) and government expenditure on housing development – in 17 countries (the biggest influence is in Romania, the Slovak Republic, Ireland, and Lithuania). It is also emphasized that government expenditure on housing development influences economic growth more significantly and with higher strength than government expenditure on housing within social protection. The obtained results can be useful in further research and government decision-making in social and economic policy, particularly regarding the expediency of increasing government spending for affordable housing and its development according to social protection programs, poverty reduction, and inclusive economic growth. Acknowledgments The study is funded by the Ministry of Education and Science of Ukraine (No. 0122U000781) and the Vega Agency (No. 1/0638/23).

The study presents the results of the correlation between students’ predictive competence and their personal characteristics. The relevance and novelty of the research results are attributed to the specification of the problematic issue connected with predictive competence. The latter is determined through the students’ subjective characteristics which are associated with self-assessment of academic achievements in the examination situation. The developed predictive competence is one of the acmeological invariants of professionalism and a resource that increases stress resistance. The aim of the research is to study the correlation between personality traits of students of the youth age period and the adequacy of their self-assessment of the academic achievements in a stressful examination situation. The study hypothesizes that personal characteristics of students determine the adequacy level of their self-assessment of the academic results and reduce the level of examination stress. The sample of the study comprises 287 first- and second-year students of the youth age period, majoring in natural sciences and socio-economic educational areas and specialties of Udmurt State Agrarian University (Izhevsk) (their average age is 18.5; 45% are girls, 55% are boys). The methods (tools) of the research are as follows: in order to fix the components of self-assessment of academic achievements and the level of mental tension of young people in the examination situation, the authors used the Dembo – Rubinstein technique (in the original modification); to determine the features of predictive skills, the “Anticipatory Consistency Test” developed by V. D. Mendelevich (ACT) was used; to measure the manifestations of anxiety as a personal quality that affects the stress level during examinations, the “The Taylor Manifest Anxiety Scale” was applied (adapted by T. A. Nemchin, V. G. Noskaridze), to measure the level of the control locus the “Rotter’s Locus of Control Scale” (adapted by E. F. Bazhin, E. A. Golynkina, A. M. Etkind) was used. The study results. Positive correlation between the locus of control and personal anxiety and negative cor- relation between the predictive competence and situational tension are characteristic of students with an adequate self-assessment of academic achievements. Developed predictive competence reduces the exam stress. The conclusion. Adequate self-assessment of academic achievements in a stressful situation contributes to the management of one’s own activities and to a better manifestation of competencies while performing intellectual tests. The obtained results are used to improve the personality-oriented program for the development of adequate self-assessment of academic achievements which serves as a stress resistance resource of students of the youth age period.

One of the modern problems in the field of ecology is the creation of environmentally friendly equipment for monitoring and maintaining trees in parks and forests. The traditional use of forest machines and self-propelled tractors with internal combustion engines has a negative impact on the environment as a result of pollutant emissions, i.e., combustion products and fuel residues. An alternative to this tradition can be the use of mobile robots with remote control of their electric drives when performing such technological operations as pruning bacterial growths of trees and diagnosing the state of tree massifs. The article proposes a fundamentally new mobile robot design for monitoring park trees. The main differences between the robot are the new designs of the body and the walking mechanisms of the mobile robot. These design differences provide the robot with high maneuverability when choosing the path of movement along the tree trunk and reliable holding of the robot body on the tree at a sufficient movement speed to perform diagnostics of the state of tree massifs. The article also describes the dynamic models of the movement of a mobile robot along a tree trunk. It presents the simulation results in the form of graph-analytical dependencies of the robot parameters, which constitutes the scientific aspect of the problem. The main motivation of the conducted research is the creation of environmentally friendly equipment in the form of a mobile robot with a reliable system of retention on the surface moving and sufficient performance to perform park tree monitoring operations.

The article examines the features of ecological tourism as a modern component of world tourism. Various scientific and practical approaches to the definition of this concept are analyzed. The functions, main directions and models of ecological tourism development, the mutual influence of ecological tourism and the environment are characterized. Also its role in the sustainable development of territories is analyzed. The methodology is based on general scientific and special research methods, including: analysis, synthesis, generalisation, retrospective analysis and geographical research methods. The article's scientific novelty lies in its comprehensive study of ecological tourism as a contemporary trend in global tourism development and its impact on regional sustainable development. It presents the author's vision of the impact of ecotourism on the surrounding anthropogenic and natural environments. Additionally, it investigates the distinctive characteristics of ecological tourism development in Ukraine. Conclusions. Ecotourism is aimed at protecting the environment and stimulating the economy of local communities. It provides tourists with the opportunity to communicate with nature and encourages them to lead an environmentally friendly lifestyle. Ecotourism also seeks to reduce the impact of tourism on the natural environment.

The article studies the features of film tourism as an innovative form of cultural and educational tourism. The relationship between the development of the global film industry and this type of tourism is analysed. The different types of impact of film tourism on tourist destinations and countries in general are characterised. The methodology is based on general scientific and special research methods, including: analysis, synthesis, generalisation, retrospective analysis and geographical research methods. Scientific novelty is a comprehensive study of film tourism as an innovative direction of development of world tourism and its impact on various aspects of life in the settlements where it has developed. The authors of the article present their vision of the impact of film tourism on the surrounding anthropogenic and natural environment. The peculiarities of the development of film tourism in Ukraine are examined. Conclusions. Film tourism is an innovative and promising area of global tourism development. It has a strong base for development in many countries of the world, both natural and artificially created, and its undoubted advantage is that the list of film attractions and destinations is expanding with every successful film premiere. Cinema tourism can be attractive to different age groups of tourists, but they are all united by a generally high level of education, relatively high incomes and a penchant for cultural leisure. The development of film tourism has a positive impact on both tourist destinations and countries.

In this study, the researchers investigated the effects of shared and different first language (L1) background, L1 use amounts, and peer familiarity on the frequency of language-related episodes (LREs) and task scores in interactive pair work. By exploring these variables in distinct interactions, the authors attempted to obtain a more fine-grained picture of peer interaction patterns and develop strategies to facilitate short interactive tasks. The study's recordings of the same-L1 dyads were translated to explore whether the participants stayed on task and why they used their L1. Forty-two intermediate students of English as a second language (ESL) from various L1 backgrounds recruited from a U.S. university participated in the study. They were placed into four dyad types: same-L1/familiar, same-L1/unfamiliar, different-L1/familiar, and different-L1/unfamiliar. Each dyad completed a short interactive spot-the-difference task. Peer familiarity positively affected the production of LREs and task scores, but no clear differences were found between the same-L1 and different-L1 dyads. The researchers also found evidence that peer familiarity affected the same-L1 and different-L1 dyads differently. The dyads stayed on task the entire time, using L1 for task management or vocabulary deliberations. Finally, strong negative correlations were found between L1 use amounts and both LREs and task scores.

This paper is devoted to providing affordable housing as a significant factor in public health management, inclusive growth, and SDG. The purpose is to empirically prove changes in the system of country’s population health care depending on the level of providing affordable housing. The empirical base includes time series and panel data for 27 EU countries during 2011–2019. Due to correlation analysis (Shapiro-Wilk testing, Spearman or Pearson correlation, lags in time), regression analysis, and building a dynamic panel estimation model with Sargan testing in STATA, the study empirically confirmed and formalized the impact of affordable housing funding on changes in the system of country’s population health care. In particular, the study found the dependence between overcrowding level and the share of homeowners with mortgages (a decrease of overcrowding level by an average of 0.61% with a time lag of 2 years due to an increase by 1%); the share of tenants on concessional terms/free (0.41% with a time lag of 3 years); and the share of public spending on housing development (0.25% with a time lag of 3 years). The direct relationship between the overcrowding and mortality from dangerous diseases (tuberculosis, AIDS, viral hepatitis, mental and behavioral disorders, diabetes, pneumonia) was also revealed. Public spending on housing under social protection programs (subsidies, etc.) proved to be the least effective. Preference should be given to the development of affordable mortgage lending (faster and greater effect). Generally, it impacts public management decisions in the health care system, social, and housing spheres. AcknowledgmentsThe study was funded by the Ministry of Education and Science of Ukraine and performed the results of the project “Economic and mathematical modeling and forecasting, development of methodological and methodological foundations for creating a roadmap for reforming the health care system in Ukraine, taking into account behavioral, social, economic and legal determinants” (Agreement БФ / 24-2021).This study received funding under the research subsidy of the Department of Applied Social Sciences of the Faculty of Organization and Management of the Silesian University of Technology for the year 2022 (13/020/BK_22/0072).

Hypophosphatasia (HPP) is a rare inherited disorder characterized by the decreased activity of tissue-nonspecific alkaline phosphatase (TNSALP), caused by mutations in the ALPL gene. The aim of this study was to conduct differential diagnostics in HPP patients using whole-exome sequencing (WES). The medical records of HPP patients and the genetic testing of the ALPL gene were reviewed. Seven patients were recruited and underwent WES using the Illumina or MGI sequencing platforms. All of the exome samples were matched onto a GRCh38.p13 reference genome assembly by using the Genome Analysis ToolKit (GATK) and the BWA MEM read aligner. We present the clinical and molecular findings of the seven patients referred for genetic analyses due to a clinical and biochemical suspicion of HPP. In two patients out of three (with identified heterozygous variants in the ALPL gene), we also identified c.682T>A in exon 3 of the WNT10A gene and c.3470del in exon 23 of the SMC1A gene variants for the first time. In four patients, variants in the ALPL gene were not detected, but WES allowed us to identify for the first time rare variants (c.5651A>C in exon 36 of the TRIO gene, c.880T>G in exon 6 of the TRPV4 gene, c.32078-1G>T in intron 159 of the TTN gene, c.47720_47721del in exon 235 of the TTN gene, and c.1946G>A in exon 15 of the SLC5A1 gene) and to conduct differential diagnostics with HPP. Using WES, for the first time, we demonstrate the possibility of early differential diagnostics in HPP patients with other rare genetic diseases. [ABSTRACT FROM AUTHOR]

Flax (Linum usitatissimum L.) products are used in the food, pharmaceutical, textile, polymer, medical, and other industries. The creation of a pan-genome will be an important advance in flax research and breeding. The selection of flax genotypes that sufficiently cover the species diversity is a crucial step for the pan-genomic study. For this purpose, we have adapted a method based on Illumina sequencing of transcriptome libraries prepared using the Tn5 transposase (tagmentase). This approach reduces the cost of sample preparation compared to commercial kits and allows the generation of a large number of cDNA libraries in a short time. RNA-seq data were obtained for 192 flax plants (3–6 individual plants from 44 flax accessions of different morphology and geographical origin). Evaluation of the genetic relationship between flax plants based on the sequencing data revealed incorrect species identification for five accessions. Therefore, these accessions were excluded from the sample set for the pan-genomic study. For the remaining samples, typical genotypes were selected to provide the most comprehensive genetic diversity of flax for pan-genome construction. Thus, high-throughput sequencing of tagmentation-based transcriptome libraries showed high efficiency in assessing the genetic relationship of flax samples and allowed us to select genotypes for the flax pan-genomic analysis.

The aim of the research was to study the response of various flax genotypes to the treatment of plants with a sulfonylurea herbicide – Magnum (active ingredient is metsulfuron-methyl) and to identify resistant forms for use in breeding programs for herbicide resistance. In 2018-2020 an assessment of 24 varieties and promising lines of fiber flax of domestic and foreign selection for resistance to this herbicide was carried out, the treatment of crops with the preparation was carried out at the recommended consumption rate of 0.01 kg/ha in the “herringbone” phase. It has been proved that the use of this herbicide leads to uneven plant growth during the “herringbone – flowering” period, which negatively affects the yield and quality of flax fiber. The decrease in the main indicators of the productivity and quality of the fiber in the variant with the use of the herbicide in comparison with the control, depending on the genotype was: in plant height – up to 44.4 %, in the weight of the technical part – 71.1 %, in the fiber mass – 74.8 %, in «myklost» (the ratio of the technical length of stem to its diameter)– 46.3 %, in the length of the elementary fiber – 27.7 %. A high level of resistance (over 80 %) to the herbicide Magnum for all analyzed traits was shown by the varieties Atlant (Russia) and Mogilevsky (Belarus). It was shown that when treating fiber flax plants with the herbicide, as a rule, an increase in inflorescence occurs due to a decrease in the technical length of the stem. It results in rise of the main indicators of seed productivity in most of genotypes under the influence of the Magnum preparation – the number of bolls and seeds per plant (up to 3.2 times). The exception was the variety Vega 2 (Lithuania), which had a decrease in the number of bolls per plant in the variant with herbicide treatment compared with the control by 1.8 times, and in the number of seeds by 5.3 times. This varietywasalso the most sensitive to the effect of Magnum in all parameters of productivity and fiber quality. The results are consistent with the data obtained whentreating various collection samples of fiber flax with a tank mixture Magnum + Herbitox L + Miura at the recommended consumption rates (0.007 + 0.6 + 1.0 kg(l)/ha).

The paper presents the result of assessing the antiradical status of consumers (in the context of Russia) in connection with their well-being. This approach is based on a multistage study, in which the results of sociological surveys were applied, as well as estimates of the antiradical potential (ARP) of diets obtained using neural networks, bootstrapping the chemical composition of diets, and calculating reference values using mathematical models. The paper presents data collected from residents living in the territories of at least 21 regions and cities of Russia: Magadan, Saint Petersburg, Moscow, Krasnodar, Lipetsk, Vladivostok, Novosibirsk, Omsk, Voronezh, etc. A total of 1001 people were interviewed, which, according to our calculations, gives a margin of error in value of approximately 3.1%. To calculate the lack of vitamins in the diets of residents of the Russian Federation, data on the chemical composition of food products from the FNDDS database were used. The assessment of dietary habits showed a lack of vitamins below the recommended level in 73% of Russians for vitamin D, 59% for retinol, 38% for β-carotenes, 13% for vitamin E, and 6% for ascorbic acid. The study showed that at least 36% of the Russian population has a low antiradical status, while it was found that “poor” consumers are more likely to consume economically more expensive foods (in terms of their nutritional value). The “poor” segments of the population consume 180–305% more canned food and 38–68% more sweet carbonated drinks than other social groups, but their consumption of vegetables is 23–48% lower. On the contrary, “wealthy” consumers consume 17–25% more complex (varied) dishes, 10–68% more fresh vegetables, and 8–39% more fish. From the obtained values it follows that consumers with low levels of ARP in their diets are in a group with an increased probability of a number of “excess” diseases (diseases of the cardiovascular system, obesity, etc.). In general, the ARP values of food consumed for low-income segments of the population were 2.3 times lower (the ratio was calculated as the percentage of consumers below the level of 11,067 equivalents necessary for the disposal of free radicals generated in the human body per day) than for those who can afford expensive food (consumers with high income). A simple increase in consumption of unbalanced foods, in our opinion, will only contribute to the entry of these consumers into the “average diet trap”. All this makes it imperative to develop comprehensive measures to create a new concept of public catering; otherwise, we can expect a reduction in both the health of the population and the performance of the economy of the whole country.

The issue of psychological feasibility of distance learning is understudied and remains relevant both in theoretical and practical terms. The purpose of the study viewed in the article is to identify the connection between students’ preferences to study using the Internet and electronic textbooks with their personal psychological traits. Presumably, confident, self-organized students have a positive attitude towards their use, while anxious, emotionally unstable students have a negative attitude. The study involves 1st–4th year students (N = 270, aged from 17to 21years; 61.3% women) students, who specialize in Economics and Agricultural Industry in Izhevsk State Agricultural Academy. We used the following techniques: questionnaire developed by O. N. Malakhova, O. A. Zhuchenko, aimed at studying students’ preferences and Cattell’s Personality Factor Questionnaire (16-PF), form C, which has the goal to study personality traits that are significant for the research. It was found that there was a direct interconnection between students’ preference to use digital educational resources with their personal psychological traits. The study revealed that students with self-discipline and analytical mindset have a negative attitude to the use of electronic textbooks and distance learning. It was found that among the main personal psychological characteristics of students, which influence their preferences, are sensitivity and radicalism, anxiety and lack of self-discipline. We did not confirm the hypothesis put forward in the study. The applied aspect of the problem under study can be implemented in educational practice to increase the efficiency of educational process and in the development of digital training courses. The research perspectives are related to the study of teacher preferences.

The author considers the decisions of the state authorities at the all-Union and republican levels on the settlement of the “Aukhov problem” in Dagestan during the period of perestroika 1985— 1991. The authors identified the conflicts in Chapaevo in 1985 and in Novokuli in 1989 as the key points of the problem. The focus of the study includes all parties to the conflict: representatives of government bodies, public structures (organizations, fronts, movements), the media and ethnic minorities (Chechens-Akkins, Laks, Avars, Kumyks). A retrospective method of historical research is used: the sequence of actions of all parties to the conflict, the cause-and-effect relationships of existing contradictions are studied. The authors concluded that, despite the containment of the conflict within certain limits, it still turned into aggressive physical and political forms of confrontation. It is pointed out that the actions of the authorities were often thoughtful and consistent, but were protracted and slow. Separately, the prolongation of preventive measures and the lack of mechanisms to resist the already erupted conflict are noted. In conclusion, it is emphasized that the problem remains unresolved to the present, therefore it is important to study and analyze the experience and mistakes of the past.

The object of this study is a mathematical model of a synchronous electric motor, obtained on the basis of experimental data, which takes into account the temperature mode and uses artificial features to increase the accuracy of its operation. A characteristic feature of this work is that the model takes into account the temperature mode as a component of the technical-operational state of the object. The resulting mathematical model could make it possible to synthesize an optimal automatic control system in terms of the operational state of the object. The problem addressed was to increase the accuracy of the identified mathematical models by applying the approach of feature engineering. The results showed that the identification of mathematical models by the initial data leads to a low level of accuracy of the obtained models, namely 65–70 % for the first output channel, 80–85 % for the second, and 75–80 % for the third, fourth, and fifth output channels. Accordingly, building models with a higher threshold of accuracy requires the use of other, more significant data for identification. This paper reports a method for reformatting the original data into artificial features and provides results of their effectiveness in relation to the original channels. The resulting artificial features and the original features were used for further identification; the resulting mathematical model has on average higher accuracy thresholds, namely 82 %, 93 %, 88 %, 85 % for the corresponding output channels. The results prove the effectiveness of applying the principle of feature engineering since the accuracy of the resulting model is 5–10 % higher compared to the baseline. The scope of practical application of the results includes the synthesis of automatic control systems based on mathematical models of control objects obtained as a result of identification. [ABSTRACT FROM AUTHOR]

The absorption of Si films covered with an array of elliptical metal nanoparticles (NPs) of identical orientation and shape is investigated theoretically. We consider silicon films with thickness in the range of 80–200 nm. The symmetry axis of the NPs is taken perpendicular to the surface of silicon surface and their short axis has a radius of 10 nm in the plane of the Si film. A 10-nm-thick SiO2 layer separates the submonolayer NP coverage from the silicon film. The spectral properties of the considered hybrid system are studied in terms of its effective susceptibility. Specifically, the dependence of light absorption by the NPs covering the ultrathin Si film on their morphology and material composition is established. For chromium and silver NPs, the appearance of collective absorption modes in the hybrid structures is predicted.

The correction of free radical oxidation processes is one of the most promising strategies of neuroprotection in acute cerebrovascular disorders.The aim of the study is an experimental study of the neuroprotective effects of 3-hydroxypyridine and erythropoietin derivatives, as well as their combined use.Materials and methods. The study was performed on 109 male Wistar rats. The neuroprotective effect of the substances was studied on a hemorrhagic stroke model. The study drugs were administered to the animals intraperitoneally. Carbamylated darbepoetin was administered three times in advance at the dose of 100 µg/kg within intervals of 3 days, the last injection took place 1 hour before the operation (the total dose was 300 mg/kg). Etoxidol was administered once 1 hour before the surgery at the dose of 50 mg/kg. The survival rate, behavioral features and the state of the animals on the 1st, 3rd, 7th and 14th days were recorded, and the morphological assessment of the brain was carried out.Results. The investigated substances had a positive effect on both the survival rate of the animals during the first day and on the 14th day. The best survival rates on the 14th day were recorded in the group of a combined use of ethoxydol and carbamylated darbepoetin (75%). Thus, in this group of rats, a faster recovery of neurological disorders was already distinguished from the first day on. By the 7th day, more than 50% of the rats receiving the combination of the studied drugs, had had a slight neurological deficit (up to 3 points on the McGrow scale); by the 14th day there had been only minor changes in the neurological status in the rats of this group. A pronounced neuroprotective effect of the combination of 3-hydroxypyridine and erythropoietin derivatives has been confirmed by a histological examination of brain slices – a more rapid decrease in the size of perifocal edema and microcirculation disorders, less damage to neurons and glial elements, and faster processes of resorption and organization of hemorrhage. A macroscopic examination of the brain sections stained with triphenyltetrazolium chloride of the dying rats, showed that perifocal necrosis had been the main cause of high mortality in the control group after the 3rd day.Conclusion. As a result of the experiment, the nephroprotective effect of the studied derivatives of 3-hydroxypyridine and erythropoietin has been proved. Moreover, the combination of these drugs has shown a greater neuroprotective activity than their isolated use. The additive effect of these drugs was due to their action mechanism resulting from the synergism of various structures and components of the cells.

Abstract Background Flax (Linum usitatissimum L.) is grown for fiber and seed in many countries. Flax cultivars differ in the oil composition and, depending on the ratio of fatty acids, are used in pharmaceutical, food, or paint industries. It is known that genes of SAD (stearoyl-ACP desaturase) and FAD (fatty acid desaturase) families play a key role in the synthesis of fatty acids, and some alleles of these genes are associated with a certain composition of flax oil. However, data on genetic polymorphism of these genes are still insufficient. Results On the basis of the collection of the Institute for Flax (Torzhok, Russia), we formed a representative set of 84 cultivars and lines reflecting the diversity of fatty acid composition of flax oil. An approach for the determination of full-length sequences of SAD1, SAD2, FAD2A, FAD2B, FAD3A, and FAD3B genes using the Illumina platform was developed and deep sequencing of the 6 genes in 84 flax samples was performed on MiSeq. The obtained high coverage (about 400x on average) enabled accurate assessment of polymorphisms in SAD1, SAD2, FAD2A, FAD2B, FAD3A, and FAD3B genes and evaluation of cultivar/line heterogeneity. The highest level of genetic diversity was observed for FAD3A and FAD3B genes – 91 and 62 polymorphisms respectively. Correlation analysis revealed associations between particular variants in SAD and FAD genes and predominantly those fatty acids whose conversion they catalyze: SAD – stearic and oleic acids, FAD2 – oleic and linoleic acids, FAD3 – linoleic and linolenic acids. All except one low-linolenic flax cultivars/lines contained both the substitution of tryptophan to stop codon in the FAD3A gene and histidine to tyrosine substitution in the FAD3B gene, while samples with only one of these polymorphisms had medium content of linolenic acid and cultivars/lines without them were high-linolenic. Conclusions Genetic polymorphism of SAD and FAD genes was evaluated in the collection of flax cultivars and lines with diverse oil composition, and associations between particular polymorphisms and the ratio of fatty acids were revealed. The achieved results are the basis for the development of marker-assisted selection and DNA-based certification of flax cultivars.

Introduction. The drugs affecting a mitochondrial dysfunction, oxidative stresses, apoptosis and inflammation of the vascular wall, have a high potential for the prevention and treatment of atherosclerotic lesions. In this regard, the use of EPOR/CD131 heteroreceptor agonists which have a similar spectrum of pharmacological effects, is one of the promising strategies in the treatment of cardiovascular diseases.Materials and Methods. The study was carried out on 68 C57Bl/6J male mice. Atherosclerosis was simulated in transgenic animals with an endotheliospecific knockdown of the Polg gene by simulating a balloon injury and keeping on a Western diet. Then, the studied drugs were injected once every 3 days at the dose of 20 μg/kg for 27 days. On the 28-th day, the animals were euthanized and the area of atherosclerotic plaques was assessed. The gene expression associated with the processes of inflammation, antioxidant protection, apoptosis, and angiogenesis was also determined in the aortic tissues. In addition, the endothelium protective effect of peptides on primary cultures of endothelial cells of wild and transgenic Polg-D257A mice was studied.Results. No statistically significant effect of drugs on the area of lipid infiltration have been found. However, the studied peptides have significantly reduced the expression of proinflammatory genes (iNos, Icam1, Vcam1, Sele, Il6, Tnfa), the genes associated with angiogenesis (Vegfa, Kdr, and Hif1a), the expression of proapoptic factors; they decreased the Bax/Bcl-2 ratio by more than 1.5 times. In addition, when supplemented with H2 O2 in vitro, peptides dose-dependently increased endothelial cell survival.Conclusion. The erythropoietin-based peptides can be used to improve the functional state of the vascular wall against the background of atherosclerotic lesions and have a depressing effect on pathobiological processes associated with a mitochondrial dysfunction. In addition, the studied peptides have a significant endothelial protective effect in the induction of oxidative stress in vitro.

The efficacy of intraabdominal chemotherapy in management of local recurrences of colon cancer was studied. Intraabdominal intraoperative and early postoperative chemotherapy made it possible to prolong of the recurrenceless term and to improve the life quality of the patients with local recurrences of colon cancer. There was observed a tendency to increase the five-year survival.

The efficacy of intraabdomlnal chemotherapy in patients with prevailing forms of colon cancer, such as peritoneal carcinomatosis, local disseminated and local recurrent colon cancer was estimated. The comparative analysis showed that the intraabdominal chemotherapy allowed to amend the remote results of the treatment in the cases with carcinomatosis, local disseminated colon cancer and local recurrence.

The chemical composition (ions, elements, polycyclic aromatic hydrocarbons) of aerosol and gaseous impurities (SO2, HNO3, HCl, NH3) in the surface layer of the atmosphere in Barentsburg, located on the Western Svalbard island (Svalbard archipelago), is analyzed. Atmospheric aerosol and gaseous impurities brought to the Arctic from middle latitudes and deposited on snow and ice not only interact with various natural objects, but also spread to long distances with melting dirty snow and ice. Air sampling was carried out following to methodology adopted by the international networks of the atmospheric monitoring programs in South-East Asia (EANET) and Europe (EMEP). In 2011-2015, the observations of the chemical composition of the atmospheric ground layer were performed daily during the light season (April–September), and monthly from April 2016 to 2018. The largest total ion concentrations were observed in 2011–2012. Seasonal variability of ion concentrations in the aerosol was characterized by high values in the cold period (October–February) and low values in the warm one (May–June). High values of the coefficient of correlation between ions Na+ and Cl− (r = 0,93) as well as between Mg2+ and Cl− (r = 0,81) throughout the year show that the main source of the aerosol is the sea surface. The significant correlation between ions K+, NO3-, NH4+, SO42−, K+, SO42− in the polar night point to the influence of local sources: coal mining at the mine and its3 combustion at thermal power plants. Emission of polycyclic aromatic hydrocarbons and the gaseous impurities (SO2, HNO3) into the atmosphere, especially during the polar night, is also influenced by local sources. Among the elements the maximum enrichment of the aerosol was revealed for As, Cr, Zn, Mo, Cd, Sn, Sb, W, and Pb with a low content of Cd, Sn, Sb, W, and Pb in the coal, sludge and on the underlying surface. On the basis of the elemental composition of the aerosol and the back-trajectory analysis, it was shown that the air masses enriched in heavy metals come to the area of the Barentsburg settlement from middle latitudes.

In conditions of vegetative trial carried out against selective backgrounds in 2017-2019 the response of 27 flax samples to a decrease in soil acidity to neutral pHKCl was studied. The scheme of the experiment was as follows: variant I (control) − pHKCl 5.3-5.5, P2O5 − 320-340 mg/kg, K2O − 81-92 mg/kg; variant II − pHKCl 6.2, P2O5 − 312-345 mg/kg, K2O − 84-98 mg/kg. It has been shown that during the «herringbone» phase in the majority of studied flax genotypes against the background of pH 6.2, the symptoms of “physiological oppression” of flax were observed: small spots developed on the upper leaves, the plants stopped growing, the stems thickened, and the tops of severely affected plants died off. As a result, at the beginning of the growing season at plant height of 7-10 cm, most of the samples were severely affected (from 69 to 100 %). The exceptions were varieties of fibre-flax Hermes (France), Vega 2 (Lithuania), Atlant (Russia) and linseed genotypes No. 3896 (Russia) and Norlin (Canada), which had a weak and medium degree of affection (8.3-45.5 %). Moreover, these genotypes showed a high level of both biological (75-90 %) and agronomic (77.3-85.6 %) resistance in the phase of "early yellow ripeness". The identified flax collection samples can be used as sources of resistance to flax «physiological oppression» caused by stressful edaphic factors in a neutral environment. On the basis of the analysis of the main elements of fiber productivity in studied flax genotypes, it has been established that against the background of pH 6.2 the reduce in plant height was from 11.4 to 52.1 % relative to the control, weight of the technical part of the stem − from 7.2 to 83.4 % , fiber mass − from 9.6 to 85.1 %. For the first time, on the basis of hybridological analysis, an assumption was done as to the pres-ence of a strong dominant gene, controlling the resistance to high soil pH values in the Hermes (France) flax variety and the linseed line No. 3896 (Russia).

Relevance of the research. Information on composition, ice content and geochemical characteristics of frozen rocks is necessary for exploration of oil and gas condensate fields of Yamal, Gydan and Taimyr. Among the factors determining the tundra landscapes ecology of the cryolithozone, underground ice and their melting have great importance. The hydrochemical composition and rare-earth elements content in syngenetic ice wedge retain the information on the moisture sources and conditions of their formation. The main ions correlation and rare-earth elements distribution in the ice composition can be used to estimate the influence of such natural factors as precipitation, sea and volcanic aerosols and anthropogenic pollution. The main aim of the research is to identify chemical elements sources and to assess the impact of the ice type on hydrochemical composition and rare-earth elements distribution in different genetic types of ice – wedge, seasonal and firn ice. Objects: ice wedge, sediments rocks, seasonal ice, surface water, taken in expeditions Earth Cryosphere Institute, Tyumen scientific centre SB RAS in 2009–2014. Methods of chemical research: inductively coupled plasma mass spectrometry, atomic absorption spectrometry, emission photometry, chromatography. Results. It is established that marine and continental aerosols influenced the chemical composition of ice wedge studied in the coastal zone of the key areas of Belyj island, Sibiryakova island, Western Yamal and Western Taimyr. Fresh and ultrafresh syngenetic ice wedge is formed by freezing melt water, which contains snow-sorbed sea aerosols – suspended in the atmosphere solid and liquid particles. Ice wedge in the lake Sokhonto area long-distanced from the sea has no signs of marine influence according to the main ions content. Ice wedge with mineral inclusions was formed not only due to snow melt water with aerosols of continental origin, but also due to the suprapermafrost water. It was found out that lanthanide content in water-soluble form has increased in comparison with both сlark values and the content in the modern surface ice. In the most of examined syngenetic ice wedge the participation of marine aerosols is confirmed taking into account the cerium anomaly. Moreover according to the distribution of rare-earth elements the correlation between light and heavy rare-earth elements typical for ice wedge, equal to 0,9, and correlation between lanthanum and the other lanthanides, equal to 0,2, are observed. Sea ice inherits the mineralization and composition of sea water. Lake ice varies in depth and reflects seasonal changes in hydrochemical composition of ice and increases in concentration of marine aerosols in the snow covering the lake ice. Firn ice of the polar Urals has a very low mineralization, there are no impurities of sea aerosol. The natural mechanism of rare earth elements accumulation in water-soluble form in combination with iron, thorium and yttrium is implemented in ice wedge of the Holocene and Neopleistocene. Melting out ice wedge may lead to the flow of rare-earth elements in the surface water.

The aim of the study was to test whether P-αB can be positioned as a preventing and treating agent for cardiovascular diseases.Materials and methods. The study was performed on sexually mature male Wistar rats. Endothelial dysfunction was modulated by a 7-days intraperitoneal administration of L-NAME at the dose of 2.5 mg/100 g. P-αB, or erythropoietin (EPO), was used for therapy at the dose of 2.5 µg/100 g × 3 times for 7 days, the total dose was 7.5 µg/100 g. The function of endothelium was estimated by an endothelium-dependent and endothelium-independent vasodilation. In addition, a histological assessment of the abdominal aortic wall state and the analysis of eNos, Tnf and Il-1β genes expression were performed. To estimate prothrombotic properties, P-αB and EPO were administered, at the doses of 2.5 and 5 µg/100 g (3 times a day for 7 days, the total doses were 7.5 µg/100 g and 15 µg/100 g, respectively) and on the 8th day, the time of ferric (III) chloride-induced carotid artery thrombosis was estimated.Results. Theresults of the functional tests for endothelium-dependent and endothelium-independent vasodilatation, as well as the histological picture of the aorta have evidenced that P-αB and EPO do not affect L-NAME-induced hypertension but improve the endothelium function. At the same time, P-αB shows a significantly higher endothelial-protective activity, reducing the coefficient of endothelial dysfunction from 5.1±0.15 to 2.72±0.12. In addition, P-αB has significantly increased the expression of eNos and reduced the expression level of Tnf and Il-1β mRNA genes. Carrying out Ferric (III) chloride-induced carotid artery thrombosis has revealed that P-αB (5 µg/100 g × 3 times a day for 7 days, total dose was 15 µg/100 g) has a lower but statistically significant prothrombotic activity than EPO.Conclusion. P-αB can be positioned as an atheroprotector because of its ability to prevent the death of endothelial cells, as well as to reduce remodeling and proinflammatory activation of the vascular wall. However, the prothrombotic properties of P-αB limit its use as a preventing and treating agent for atherosclerosis-associated diseases.

Lake Baikal phage communities are important for lake ecosystem functioning. Here we describe the diversity of T4-bacteriophage associated with the bacterial fraction of filtered water samples collected from the pelagic zone, coastal zone and shallow bays. Although the study of the diversity of phages for the g23 gene has been carried out at Lake Baikal for more than ten years, shallow bays that comprise a significant part of the lake’s area have been neglected, and this gene has not previously been studied in the bacterial fraction. Phage communities were probed using amplicon sequencing methods targeting the gene of major capsid protein (g23) and compared phylogenetically across sample locations and with sequences previously retrieved from non-bacterial fractions (

Flax (Linum usitatissimum L.) is attacked by numerous devastating fungal pathogens, including Colletotrichum lini, Aureobasidium pullulans, and Fusarium verticillioides (Fusarium moniliforme). The effective control of flax diseases follows the paradigm of extensive molecular research on pathogenicity. However, such studies require quality genome sequences of the studied organisms. This article reports on the approaches to assembling a high-quality fungal genome from the Oxford Nanopore Technologies data. We sequenced the genomes of C. lini, A. pullulans, and F. verticillioides (F. moniliforme) and received different volumes of sequencing data: 1.7 Gb, 3.9 Gb, and 11.1 Gb, respectively. To obtain the optimal genome sequences, we studied the effect of input data quality and genome coverage on assembly statistics and tested the performance of different assembling and polishing software. For C. lini, the most contiguous and complete assembly was obtained by the Flye assembler and the Homopolish polisher. The genome coverage had more effect than data quality on assembly statistics, likely due to the relatively low amount of sequencing data obtained for C. lini. The final assembly was 53.4 Mb long and 96.4% complete (according to the glomerellales_odb10 BUSCO dataset), consisted of 42 contigs, and had an N50 of 4.4 Mb. For A. pullulans and F. verticillioides (F. moniliforme), the best assemblies were produced by Canu–Medaka and Canu–Homopolish, respectively. The final assembly of A. pullulans had a length of 29.5 Mb, 99.4% completeness (dothideomycetes_odb10), an N50 of 2.4 Mb and consisted of 32 contigs. F. verticillioides (F. moniliforme) assembly was 44.1 Mb long, 97.8% complete (hypocreales_odb10), consisted of 54 contigs, and had an N50 of 4.4 Mb. The obtained results can serve as a guideline for assembling a de novo genome of a fungus. In addition, our data can be used in genomic studies of fungal pathogens or plant–pathogen interactions and assist in the management of flax diseases.

The article presents an overview of the urban development situation in the city of Tyumen over a century of Soviet and post-Soviet construction. The city with a fairly impressive number of objects to be proud of, and with a historical layout that is of special interest, has developed a number of problems, particularly in its transport infrastructure, which must be addressed as soon as possible. The authors offer several options for solving the acute urban planning issues by changing the transport situation both on a city-wide scale and on the example of two iconic urban areas.

Flax is grown worldwide for seed and fiber production. Linseed varieties differ in their oil composition and are used in pharmaceutical, food, feed, and industrial production. The field of application primarily depends on the content of linolenic (LIN) and linoleic (LIO) fatty acids. Inactivating mutations in the FAD3A and FAD3B genes lead to a decrease in the LIN content and an increase in the LIO content. For the identification of the three most common low-LIN mutations in flax varieties (G-to-A in exon 1 of FAD3A substituting tryptophan with a stop codon, C-to-T in exon 5 of FAD3A leading to arginine to a stop codon substitution, and C-to-T in exon 2 of FAD3B resulting in histidine to tyrosine substitution), three approaches were proposed: (1) targeted deep sequencing, (2) high resolution melting (HRM) analysis, (3) cleaved amplified polymorphic sequences (CAPS) markers. They were tested on more than a thousand flax samples of various types and showed promising results. The proposed approaches can be used in marker-assisted selection to choose parent pairs for crosses, separate heterogeneous varieties into biotypes, and select genotypes with desired homozygous alleles of the FAD3A and FAD3B genes at the early stages of breeding for the effective development of varieties with a particular LIN and LIO content, as well as in basic studies of the molecular mechanisms of fatty acid synthesis in flax seeds to select genotypes adequate to the tasks.

The method of numerical modeling of wave scattering by periodic impedance grating is considered. In the case of a harmonic dependence of the field on time and the uniformity of the structure along a certain axis, the three-dimensional problem reduces to considering of two 2D problems for the components of the E-polarized and H-polarized waves. The signle nonzero component of the electric field created by the incident E-polarized wave is the solution of the boundary value problem for the Helmholtz equation with Robin boundary conditions. It follows from the physical formulation of the problem that its solutions satisfy the Floquet quasiperiodicity condition, the condition of finiteness of energy in any bounded region of the plane. Also, the difference between the total and incident fields satisfies the Sommerfeld radiation condition. Following the ideas of the works of Yu.V. Gandel, using the method of parametric representations of integral operators, the boundary-value problem reduces to two systems of integral equations. The first one is the system of singular equations of the first kind with additional integral conditions. The second system consists of the Fredholm boundary integral equations of the second kind with a logarithmic singularity in the integrand. A discrete model for various values of the discretization parameter is equivalent to systems of singular integral equations. By solving these equations, approximate values of the main field characteristics are determined. The method of parametric representations of integral operators makes it possible to obtain systems of integral equations of other types. In particular, the initial boundary-value problem reduces to a system consisting of hypersingular integral equations of the second kind and the Fredholm integral equation of the second kind. A numerical experiment was conducted for cases of different location of tapes. Calculations were performed for the proposed model and the model based on hypersingular equations. They showed the closeness of the obtained results in a wide range of parameters studied.

The article is aimed at researching the tools and parameters of assessing the effectiveness of innovation activity, increasing the competence of tourism enterprises, improving the competitiveness of innovative enterprises, international tour-operator-ranking. The system of tourism multipliers is substantiated, which special constituents are: an innovation multiplier, which represents an increase in income in the tourism economy, possible as a result of the implementation of a new product, changes in service technology, in the marketing, process, service, organizational or other innovations; also the company’s sustainability growth multiplier as an indicator of the estimated business reputation of the tourism company as part of the overall corporate strategy, market share and participation in the distribution/redistribution of tourist flows.

Flax (Linum usitatissimum L.) is used for the production of textile, oils, pharmaceuticals, and composite materials. Fusarium wilt, caused by the fungus Fusarium oxysporum f. sp. lini, is a very harmful disease that reduces flax production. Flax cultivars that are resistant to Fusarium wilt have been developed, and the genes that are involved in the host response to F. oxysporum have been identified. However, the mechanisms underlying resistance to this pathogen remain unclear. In the present study, we used transcriptome sequencing data obtained from susceptible and resistant flax genotypes grown under control conditions or F. oxysporum infection. Approximately 250 million reads, generated with an Illumina NextSeq instrument, were analyzed. After filtering to exclude the F. oxysporum transcriptome, the remaining reads were mapped to the L. usitatissimum genome and quantified. Then, the expression levels of cinnamyl alcohol dehydrogenase (CAD) family genes, which are known to be involved in the response to F. oxysporum, were evaluated in resistant and susceptible flax genotypes. Expression alterations in response to the pathogen were detected for all 13 examined CAD genes. The most significant differences in expression between control and infected plants were observed for CAD1B, CAD4A, CAD5A, and CAD5B, with strong upregulation of CAD1B, CAD5A, and CAD5B and strong downregulation of CAD4A. When plants were grown under the same conditions, the expression levels were similar in all studied flax genotypes for most CAD genes, and statistically significant differences in expression between resistant and susceptible genotypes were only observed for CAD1A. Our study indicates the strong involvement of CAD genes in flax response to F. oxysporum but brings no evidence of their role as resistance gene candidates. These findings contribute to the understanding of the mechanisms underlying the response of flax to F. oxysporum infection and the role of CAD genes in stress resistance.