1. Rapid and Sensitive Detection of Influenza B Virus Employing Nanocomposite Spheres Based on Ag-Doped ZnIn 2 S 4 Quantum Dots.
- Author
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Hu, Jia-Xuan, Zhu, Li-Bang, Wu, Sheng-Tong, and Ding, Shou-Nian
- Subjects
INFLUENZA B virus ,QUANTUM dots ,DOPING agents (Chemistry) ,COLLOIDAL gold ,SILICA nanoparticles - Abstract
Lateral flow immunoassay (LFIA) technology serves a significant role as a simple and rapid biosensor in the detection of influenza viruses. The focus of this study is the development of a rapid and convenient screening method for influenza B virus (IBV) proteins using a fluorescence lateral flow biosensor based on Ag-doped ZnIn
2 S4 quantum dots (Ag: ZIS QDs) as signal reporters. These Ag: ZIS QDs-emitting orange fluorescence are loaded onto dendritic mesoporous silica nanoparticles (DMSNs) and are further coated with a layer of silica shell to form a core–shell structured composite nanomaterial (SiO2 @ Ag: ZIS QDs @ DMSNs). The orange fluorescence effectively eliminates the interference of blue background fluorescence, significantly enhancing the detection sensitivity. This technology demonstrates outstanding performance in the immediate detection of IBV, with a minimum detection limit of 1 ng/mL, compared to the traditional colloidal gold strip with a detection limit of 6 ng/mL. Furthermore, both intra-assay and inter-assay coefficients of variation (CV) are less than 9%. This method holds promise for wide application in early diagnosis, epidemiological investigation, and epidemic surveillance of IBV. [ABSTRACT FROM AUTHOR]- Published
- 2024
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