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Acute lung injury (ALI) is a severe respiratory disease with a high mortality rate. The integrity of the pulmonary endothelial barrier influences the development and prognosis of ALI. Therefore, it has become an important target for ALI treatment. Extracellular vesicles (EVs) are promising nanotherapeutic agents against ALI. Herein, endothelium‐derived engineered extracellular vesicles (eEVs) that deliver microRNA‐125b‐5p (miRNA‐125b) to lung tissues exerting a protective effect on endothelial barrier integrity are reported. eEVs that are modified with lung microvascular endothelial cell‐targeting peptides (LET) exhibit a prolonged retention time in lung tissues and targeted lung microvascular endothelial cells in vivo and in vitro. To improve the efficacy of the EVs, miRNA‐125b is loaded into EVs. Finally, LET‐EVs‐miRNA‐125b is constructed. The results show that compared to the EVs, miRNA‐125b, and EVs‐miRNA‐125b, LET‐EVs‐miRNA‐125b exhibit the most significant treatment efficacy in ALI. Moreover, LET‐EVs‐miRNA‐125b is found to have an important protective effect on endothelial barrier integrity by inhibiting cell apoptosis, promoting angiogenesis, and protecting intercellular junctions. Sequencing analysis reveals that LET‐EVs‐miRNA‐125b downregulates early growth response‐1 (EGR1) levels, which may be a potential mechanism of action. Taken together, these findings suggest that LET‐EVs‐miRNA‐125b can treat ALI by protecting the endothelial barrier integrity. [ABSTRACT FROM AUTHOR]

Flexible, lightweight, and cost‐effective organic light‐emitting diodes (OLEDs) show great promise for use in displays and lighting applications. However, creating highly efficient and stable deep‐blue OLEDs remains a challenge due to a lack of efficient functional materials, specifically hole transport materials (HTM). In this report, two new spiro‐HTMs are introduced with fluorene (FBP‐SFX) or dibenzofuran (DBF‐SFX) peripheral substituents. This study finds that the DBF‐SFX variant produces highly efficient deep‐blue OLEDs with a narrow emission (full‐width at half‐maximum FWHM of 25 nm) and the highest external quantum efficiency (EQE) of 33.2% among the FBP‐SFX (12.2%) and NPB (11.9%) variants. The superior performance of DBF‐SFX is attributed to its deeper highest occupied molecular orbital level (HOMO) and small trap density, which results in reduced band‐edge offset, lower turn‐on voltages, and balanced charge carrier densities. Importantly, the blue fluorescence OLED with DBF‐SFX displays exceptional device stability with LT95 over 420 h with an initial luminance of 1000 cd m−2. This is due to the stronger bond dissociation energy (BDE) and suppressed Exciton–Polaron Annihilation (EPA) using the dibenzofuran substituent. This work demonstrates a new HTM design approach using a spiro‐core architecture and dibenzofuran substituents to create efficient and long‐lasting deep‐blue OLEDs. [ABSTRACT FROM AUTHOR]

Multiple resonance thermally activated delayed fluorescence (MR-TADF) emitters have immense potential for use in the development of high color purity and high-resolution organic light-emitting diode (OLED) displays. However, the scarcity of high-performance narrowband blue emitters has been a challenge. Herein, three blue MR-TADF emitters have been synthesized using halogen borylation. The introduction of dibenzofuran to extend the π-conjugate skeletons has resulted in emitters with exceptional properties such as large reverse intersystem crossing (RISC) rates, a high photoluminescence quantum yield (PLQY) of 92% and a small full width at half maximum (FWHM) value of 25 nm. The electroluminescent devices utilizing DABNA-3-DBF as emitters have displayed remarkable external quantum efficiencies (EQEs) of 25.9% and Commission Internationale de l'Eclairage (CIE) coordinates of (0.16, 0.07). This work presents a viable strategy for the development of high-performance blue MR-TADF with a narrow FWHM. [ABSTRACT FROM AUTHOR]

Sulfur mustard (SM) is a blister‐producing chemical warfare agent which could lead to a cascade of systemic damage, especially severe acute lung injury. Oxidative stress is considered to be vital processes for the SM toxicity mechanism. We previously proved the therapeutic effect of exosomes derived from bone marrow mesenchymal stromal cells in promoting the repair of alveolar epithelial barrier and inhibiting apoptosis. However, the key functional components in exosomes and the underlying mechanisms have not been fully elaborated. This research shed light on the function of the key components of human umbilical cord mesenchymal stem cell‐derived exosomes (HMSCs‐Ex). We noted that HMSCs‐Ex‐derived miR‐199a‐5p played a vital role in reducing pneumonocyte oxidative stress and apoptosis by reducing reactive oxygen species, lipid peroxidation products and increasing the activities of antioxidant enzymes in BEAS‐2B cells and mouse models after exposure to SM for 24 h. Furthermore, we demonstrated that the overexpression of miR‐199a‐5p in HMSCs‐Ex treatment induced a further decrease of Caveolin1 and the activation of the mRNA and protein level of NRF2, HO1 and NQO1, compared with HMSCs‐Ex administration. In summary, miR‐199a‐5p was one of the key molecules in HMSCs‐Ex that attenuated SM‐associated oxidative stress via regulating CAV1/NRF2 signalling pathway. [ABSTRACT FROM AUTHOR]

Background: Sulfur mustard (SM) is a highly toxic chemical warfare agent that has caused numerous casualties during wars and conflicts in the past century. Specific antidotes or therapeutic strategies are rare due to the complicated mechanism of toxicity, which still awaits elucidation. Clinical data show that acute lung injury (ALI) is responsible for most mortality and morbidity after SM exposure. Extracellular vesicles are natural materials that participate in intercellular communication by delivering various substances and can be modified. In this study, we aim to show that extracellular vesicles derived from human umbilical cord mesenchymal stromal cells (hucMSC-EVs) could exert therapeutic effects on SM-induced ALI, and to explain the underlying mechanism of effects. Methods: MiR-146a-5p contained in hucMSC-EVs may be involved in the process of hucMSC-EVs modulating the inflammatory response to SM-induced ALI. We utilized miR-146a-5p delivered by extracellular vesicles and further modified hucMSCs with a miR-146a-5p mimic or inhibitor to collect miR-146a-5p-overexpressing extracellular vesicles (miR-146a-5p+-EVs) or miR-146a-5p-underexpressing extracellular vesicles (miR-146a-5p−-EVs), respectively. Through in vivo and in vitro experiments, we investigated the mechanism. Results: The effect of miR-146a-5p+-EVs on improving the inflammatory reaction tied to SM injury was better than that of hucMSC-EVs. We demonstrated that miR-146a-5p delivered by hucMSC-EVs targeted TRAF6 to negatively regulate inflammation in SM-induced ALI models in vitro and in vivo. Conclusion: In summary, miR-146a-5p delivered by hucMSC-EVs targeted TRAF6, causing hucMSC-EVs to exert anti-inflammatory effects in SM-induced ALI; thus, hucMSC-EVs treatment may be a promising clinical therapeutic after SM exposure. [ABSTRACT FROM AUTHOR]

There is little investigation into the impact of molecular conformation on device efficiency and degradation of boron‐nitrogen thermally activated delayed fluorescence emitters (BN‐TADF). Herein, three highly‐efficient green BN‐TADF emitters have been designed to unveil the impact of peripheral phenyl groups on device efficiencies and lifetimes. Compared to BN‐PhOH with the lowest EQEmax of 19 %, BN‐PhOCH3 and BN‐PhN(CH3)2 have achieved strongly enhanced EQEmax of 25.6 % and 24.1 %, respectively. Importantly, the device lifetimes (LT50) are dramatically improved from 1.7 h of BN‐PhOH to 4.4 h of BN‐PhOCH3 and 7.7 h of BN‐PhN(CH3)2 without encapsulation. According to in situ Raman spectroscopy and simulations, BN‐PhN(CH3)2 of less conformation change after aging exhibits the best photostability. It is proposed that the torsion angle change between the BN core and the peripheral phenyl group results in BN‐TADF degradation. This knowledge means precisely tuning peripheral groups of BN‐TADF can achieve both higher device efficiencies and longer lifetimes. [ABSTRACT FROM AUTHOR]

The efficient operation of Intelligent Warehousing System does not rely on individual resource scheduling in stages but multi-type resources collaborative scheduling. In this paper, a collaborative scheduling model for stackers, automated guided vehicles and picking workstations in outbound process is abstracted into a hybrid flow-shop scheduling problem within an automated warehouse scene. Considering the impacts of uncertain factors related to scheduling, the objective function of this model is minimizing the makespan based on the triangular fuzzy processing time. A genetic algorithm is designed to obtain feasible solution of this model with the form of vector coding and the approach of ranking fuzzy numbers. Example analysis shows that the validity of the model and algorithm is verified. Within different resource allocation schemes, their evaluating indexes are significantly different, which are the likely completion time of system operation, the capability coordination degree and the initial investment. Furthermore, the increase of picking workstations is contributed much more to reducing the likely completion time and to improving the capability coordination degree than that of automated guided vehicles. [ABSTRACT FROM AUTHOR]

Barnacles are notorious marine fouling organisms. Their successful attachment to a substrate requires that they search for an appropriate habitat during their cyprid stage. A chemical cue called SIPC (Settlement-Inducing Protein Complex) has been shown to play a key role in the induction of cyprid gregarious settlement; however, the underlying biochemical mechanism remains unclear. Here, RNA-seq was used to examine the gene expression profiles of Amphibalanus amphitrite cyprids in response to SIPC and to identify SIPC-activated intracellular signaling pathways. A total of 389 unigenes were differentially expressed in response to SIPC, and cement protein genes were not among them. KEGG enrichment analysis suggested that SNARE interactions in the vesicular transport pathway were significantly influenced by SIPC treatment, indicating a possible role for SIPC in triggering protein transportation and secretion. Several genes with specific functions in metamorphosis were found among the differentially expressed genes (DEGs). GO (Gene Ontology) enrichment analysis revealed that the DEGs were significantly enriched in enamel mineralization pathways, suggesting that SIPC may also be involved in the activation of mineralization. • We observed the behaviors of cyprid antennules in SIPC supplemented FSW. • The expression of cement protein genes was not remarkably influenced by SIPC treatment. • SIPC induces highly expression of genes involved in SNARE interactions in the vesicular transport pathway. • GPCR may initiate the signal transduction cascade leading to settlement. [ABSTRACT FROM AUTHOR]

Biomineralization induced by microorganisms has become a hot spot in the field of carbonate sedimentology; however, the mechanisms involved still need to be explored. In this study, the bacterium Bacillus subtilis J2 (GenBank MG575432) was used to induce the precipitation of calcium carbonate minerals at Mg/Ca molar ratios of 0, 3, 6, 9, and 12. Bacillus subtilis J2 bacteria released ammonia to increase pH, but the ammonia released only made the pH increase to 8.25. Carbonic anhydrase was also produced to catalyze the hydration of carbon dioxide, and this process released carbonate and bicarbonate ions that not only increased pH but also elevated carbonate supersaturation. The biominerals formed at a Mg/Ca molar ratio of 0 were spherulitic, elongated, dumbbell-shaped, and irregularly rhombohedral calcite; at a Mg/Ca molar ratio of 3, the biominerals were calcite and aragonite, the weight ratio of calcite decreased from 26.7% to 15.6%, and that of aragonite increased from 73.3% to 84.4% with increasing incubation time. At higher Mg/Ca molar ratios, the biominerals were aragonite, and the crystallinity and thermal stability of aragonite decreased with increasing Mg/Ca molar ratios. FTIR results showed that many organic functional groups were present on/within the biominerals, such as C–O–C, N–H, C=O, O–H, and C–H. HRTEM-SAED examination of the ultra-thin slices of B. subtilis J2 bacteria showed that nano-sized minerals with poor crystal structure had grown or been adsorbed on the EPS coating. The EPS of the B. subtilis J2 strain contained abundant glutamic acid and aspartic acid, which could be deprotonated in an alkaline condition to adsorb Ca2+ and Mg2+ ions; this made EPS act as the nucleation sites. This study may provide some references for further understanding of the mechanism of biomineralization induced by microorganisms. [ABSTRACT FROM AUTHOR]

Barnacles robustly adhere themselves to diverse submarine substrates through a proteinaceous complex termed the “barnacle cement”. Previous studies have indicated that certain peptides derived from some barnacle cement proteins can self-assemble into amyloid fibrils. In this study, we assessed the self-assembly behavior of a full-length 19 kDa cement protein from Balanus albicostatus ( Bal cp19k) in different buffers. Results of Thioflavin T binding assay, transmission electron microscopy, and Fourier transform infrared spectroscopy suggested that the bacterial recombinant Bal cp19k was able to aggregate into typical amyloid fibrils. The time required for the self-assembly process was close to that required for the complete curing of barnacle cement complex. Moreover, the solubility of Bal cp19k amyloid deposits in guanidine hydrochloride and urea was same as that of the cured cement. These results indicated the inherent self-assembling nature of Bal cp19k, implying that the amyloid fibril formation plays a critical role in barnacle cement curing procedure and its insolubility. Our results should be conducive to understanding barnacle underwater adhesion mechanisms and have implications in the development of new-generation antifouling techniques and in the designing of novel wet adhesives for biomedical and technical applications. [ABSTRACT FROM AUTHOR]

• Reduced terrestrial deposition in the Sunda Shelf since the last glaciation. • Remarkable mangrove peaks during meltwater pulse (MWP) events in Sunda Shelf. • Increased mangrove deposition and p CO 2 during the strong MWP events 1A and 1B. Mangroves have received increasing attention in recent years for their high carbon storage capacity. The effects of sea-level change during the last glacial period on terrestrial deposition and coastal mangrove ecosystems were investigated using various organic lipid proxies in marine sediment cores from the Sunda Shelf in the southern South China Sea (SCS). The gradual reduction in the content of long-chain n -alkanes and n -alkanols, BIT index, and increasing δ13C org trend from the last glacial to the Holocene indicated that the depocenter moved landward from the studied location during the deglacial sea-level rise. Remarkable peaks in the mangrove proxy (Taraxerol/ n -C 28 alcohol ratio) occurred during meltwater pulse (MWP) events in the Sunda Shelf area, associated with the drowning and destruction of mangroves that could not withstand the rapid sea-level rise. The decomposition of carbon-rich mangrove deposits may have contributed to atmospheric CO 2 concentration during two strong MWP events in Sunda Shelf. Our results suggest the vulnerability of mangrove systems upon rapid sea level change with positive feedback for global warming. [ABSTRACT FROM AUTHOR]

In recent years, the integration of robots in minimally invasive surgery has gained significant traction in clinical practice. However, conventional contact-based human-computer interaction poses the risk of bacterial infection, significantly limiting the role of robots in surgery. To address this limitation, we propose an innovative interaction method rooted in gestures and visual tags, allowing surgeons to control and fine-tune surgical robots without physical contact with the environment. By encoding the six gestures collected using LeapMotion, we can effectively control the surgical robot in a non-contact manner. Moreover, utilizing Aruco technology, we have accurately identified the 3D spatial position of the visual label, and developed 12 fine-tuning operations to refine surgical instruments. To evaluate the applicability of our proposed system in surgery, we designed a relevant experimental setup. In the experiment, we achieved enough precision. These results demonstrate that our system meets the clinical standard, providing doctors with a non-contact and flexible means of interacting with robots during surgery., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Zhang, Wang, Dai, Shen and Chen.)

Robot-assisted minimally invasive surgery (RAMIS) has gained significant traction in clinical practice in recent years. However, most surgical robots rely on touch-based human-robot interaction (HRI), which increases the risk of bacterial diffusion. This risk is particularly concerning when surgeons must operate various equipment with their bare hands, necessitating repeated sterilization. Thus, achieving touch-free and precise manipulation with a surgical robot is challenging. To address this challenge, we propose a novel HRI interface based on gesture recognition, leveraging hand-keypoint regression and hand-shape reconstruction methods. By encoding the 21 keypoints from the recognized hand gesture, the robot can successfully perform the corresponding action according to predefined rules, which enables the robot to perform fine-tuning of surgical instruments without the need for physical contact with the surgeon. We evaluated the surgical applicability of the proposed system through both phantom and cadaver studies. In the phantom experiment, the average needle tip location error was 0.51  mm, and the mean angle error was 0.34 degrees. In the simulated nasopharyngeal carcinoma biopsy experiment, the needle insertion error was 0.16  mm, and the angle error was 0.10 degrees. These results indicate that the proposed system achieves clinically acceptable accuracy and can assist surgeons in performing contactless surgery with hand gesture interaction., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Wang, Zhang, Chen and Song.)

Introduction: Breast cancer (BC) is the most common malignancy in the world and has a high cancer-related mortality rate. Basement membranes (BMs) guide cell polarity, differentiation, migration and survival, and their functions are closely related to tumor diseases. However, few studies have focused on the association of basement membrane-related genes (BMRGs) with BC. This study aimed to explore the prognostic features of BMRGs in BC and provide new directions for the prevention and treatment of BC., Methods: We collected transcriptomic and clinical data of BC patients from TCGA and GEO datasets and constructed a predictive signature for BMRGs by using univariate, least absolute shrinkage and selection operator (LASSO) and multivariate Cox regression analysis. The reliability of the model was further evaluated and validated by Kaplan-Meier survival curves and receiver operating characteristic curves (ROC). Column line plots and corresponding calibration curves were constructed. Possible biological pathways were investigated by enrichment analysis. Afterward, we assessed the mutation status by tumor mutational burden (TMB) analysis and compared different subtypes using cluster analysis. Finally, we examined drug treatment sensitivity and immunological correlation to lay the groundwork for more in-depth studies in this area., Results: The prognostic risk model consisted of 7 genes (FBLN5, ITGB2, LAMC3, MMP1, EVA1B, SDC1, UNC5A). After validation, we found that the model was highly reliable and could accurately predict the prognosis of BC patients. Cluster analysis showed that patients with cluster 1 had more sensitive drugs and had better chances of better clinical outcomes. In addition, TMB, immune checkpoint, immune status, and semi-inhibitory concentrations were significantly different between high and low-risk groups, with lower-risk patients having the better anti-cancer ability., Discussion: The basement membrane-related gene signature that we established can be applied as an independent prognostic factor for BC and can provide a reference for individualized treatment of BC patients., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Cai, Zhang, Xie, Li, Liu and Liu.)

Over the past 2 years, the world has witnessed the devastating effects of the COVID-19 pandemic on humanity. Fortunately, stem cell therapy is widely used in clinical practice for the treatment of COVID-19 and has saved the lives of many critically ill patients. A bibliometric analysis of this field can analyze research hotspots and predict the research trends. This research analyzed documents from Web of Science between the years 2020-2022. The bibliometrics software bibliometrix, VOSviewer, and CiteSpace were used to complete the visual analysis of publications, authors, countries, documents, organizations, collaborative networks, and keywords clustering. 896 publications on COVID-19 stem cell therapy were included in the analysis, including 451 articles and 445 review articles. The field grew at the average growth rate of 103.17% between 2020 and 2021. The United States had the highest number of publications and citations. Many developing countries had also contributed significantly to the field. The journal with the most articles was Stem Cell Research and Therapy . The most cited journal was Stem Cell Reviews and Reports . The published documents were focused on five themes: "Cell Biology", "Medicine Research Experimental", "Cell Tissue Engineering", "Immunology", and "Pharmacology Pharmacy". The bibliometric analysis revealed that current clinical trials had validated stem cell therapy's remarkable potential in treating COVID-19 and its complications. It is foreseeable that future research in this area will continue to increase. With the help of bibliometric analysis, researchers can identify the current state of research and potential research hotspots., Competing Interests: The authors declare that the research was carried out independently of any commercial or financial relationships that might be considered conflicts of interest., (Copyright © 2022 Zhang, Cai, Chen, Yang, Tian, Wu, Ji, Zheng, Li and Chen.)

Breast cancer (BC), the most common malignancy in women, has a high cancer-related mortality. Endoplasmic reticulum stress (ERS), a response to the accumulation of unfolded proteins, has emerging roles in tumorigenesis, including invasion, metastasis, immune escape, etc. However, few studies have focused on the correlation between ERS with long non-coding RNAs (lncRNAs) in BC. We attempted to construct an ERS-related lncRNA prognostic signature and study its value in BC from tumor mutational burden (TMB), tumor immune microenvironment (TIME), cluster, clinical treatment, and so on. In the present study, transcriptomic and clinical data of BC patients were extracted from The Cancer Genome Atlas (TCGA) database. Correlation test, Cox regression analysis, least absolute shrinkage, and selection operator (LASSO) method were performed to determine an ERS-related lncRNA prognostic signature. Survival and predictive performance were analyzed according to Kaplan-Meier curves and receiver operating characteristic (ROC) curves, while nomograms and calibration curves were established. Then, an enrichment analysis was performed to study the functions and biological processes of ERS-related lncRNAs. TMB and TIME were also analyzed to assess the mutational status and immune status. Additionally, by using consensus cluster analysis, we compared differences among tumor subtypes. Drug sensitivity analysis and immunologic efficacy evaluations were performed together for further exploration. We identified a novel prognostic signature consisting of 9 ERS-related lncRNAs. High-risk patients had worse prognoses. The signature had a good predictive performance as an independent prognostic indicator and was significantly associated with clinicopathological characteristics. Enrichment analysis showed that metabolic pathways were enriched in high-risk patients, while immune pathways were more active in low-risk patients. Low-risk patients had lower TMB, higher immune scores, and stronger immune functions. Cluster analysis clarified that cluster 2 had the most active immune functions and was sensitive to more drugs, which may have the best clinical immunological efficacy. A clinical efficacy evaluation revealed that patients in the low-risk group may benefit more from chemotherapy, targeted therapy, and immunotherapy. The novel signature has significant clinical implications in prognosis prediction for BC. Our study clarifies that there is a potential connection between the ERS-related lncRNAs and BC, which may provide new treatment guidelines for BC., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Cai, Ji, Wu, Chen, Zheng, Chen, Zhang, Xie, Huang, Chen, Lin, Lin, Chen and Li.)

There is little investigation into the impact of molecular conformation on device efficiency and degradation of boron-nitrogen thermally activated delayed fluorescence emitters (BN-TADF). Herein, three highly-efficient green BN-TADF emitters have been designed to unveil the impact of peripheral phenyl groups on device efficiencies and lifetimes. Compared to BN-PhOH with the lowest EQE max of 19 %, BN-PhOCH 3 and BN-PhN(CH 3 ) 2 have achieved strongly enhanced EQE max of 25.6 % and 24.1 %, respectively. Importantly, the device lifetimes (LT 50 ) are dramatically improved from 1.7 h of BN-PhOH to 4.4 h of BN-PhOCH 3 and 7.7 h of BN-PhN(CH 3 ) 2 without encapsulation. According to in situ Raman spectroscopy and simulations, BN-PhN(CH 3 ) 2 of less conformation change after aging exhibits the best photostability. It is proposed that the torsion angle change between the BN core and the peripheral phenyl group results in BN-TADF degradation. This knowledge means precisely tuning peripheral groups of BN-TADF can achieve both higher device efficiencies and longer lifetimes., (© 2022 Wiley-VCH GmbH.)