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Organizational culture has always been a mainstream issue of research in the field of organizations, and its connotation has been gradually enriched, perfected and presented a trend of diversification. Innovation is perceived as a critical factor in helping enterprises to retain competitive advantages, while employee innovation behaviour is thought to be the basis for enterprises to innovate. Using the definitions proposed by Chinese and foreign scholars in the past decade for reference and from the perspective of social cognitive theory, this paper, in the form of an argumentative essay, mainly to elaborate the connotation of organizational culture as well as the concept of employee innovation behavior, and it studies on the impact mechanism of organizational culture on employees and defines the research concepts on the impact of employee innovation behavior. And therefore the paper introduces the state of related research in China and abroad by outlining and summarizing the existing research, and points out that an organization creating atmosphere of encouragement for innovation can stimulate the employee’s sense of self-efficacy and further make the individuals sense of self-reliance, sense of efficacy, which in turn leads to a sense of innovation among individual employees. This paper theoretically shows that organizational culture plays a rather positive part in guiding individual employee to display innovative behaviors, and by summarizing and reviewing the existing studies, it points out the future development prospect of this field, which will provide reference and help for the subsequent researchers to carry out their studies.

Integrating science, technology, engineering, and mathematics (STEM) education in physical education (PE) has received increased attention. PE teachers' past behaviors and behavioral intentions of integrating STEM education in their teaching were investigated in this cross-sectional survey via the lens of the theory of planned behavior (TPB). Primary and secondary school PE teachers (n = 165) completed a survey that measured their behaviors and intentions surrounding STEM. The results indicated that only 19.4% of the PE teachers sometimes or often integrated STEM education in their teaching. Attitudes, subjective norms, and perceived behavior control positively predicted PE teachers' behavioral intentions, which was consistent with the TPB. These findings support the utility of TPB in understanding PE teachers' intentions to integrate STEM education in PE classrooms. The findings also provide the first evidence-based data for policymakers to promote STEM education in PE.

The use of arbuscular mycorrhizal fungi (AMF) is a vital strategy for enhancing the phytoremediation of heavy metals. However, the role of AMF under molybdenum (Mo) stress is elusive. A pot culture experiment was conducted to explore the effects of AMF (Claroideoglomus etunicatum and Rhizophagus intraradices) inoculation on the uptake and transport of Mo and the physiological growth of maize plants under different levels of Mo addition (0, 100, 1000, and 2000 mg/kg). AMF inoculation significantly increased the biomass of maize plants, and the mycorrhizal dependency reached 222% at the Mo addition level of 1000 mg/kg. Additionally, AMF inoculation could induce different growth allocation strategies in response to Mo stress. Inoculation significantly reduced Mo transport, and the active accumulation of Mo in the roots reached 80% after inoculation at the high Mo concentration of 2000 mg/kg. In addition to enhancing the net photosynthetic and pigment content, inoculation also increased the biomass by enhancing the uptake of nutrients, including P, K, Zn, and Cu, to resist Mo stress. In conclusion, C. etunicatum and R. intraradices were tolerant to the Mo stress and could alleviate the Mo-induced phytotoxicity by regulating the allocation of Mo in plants and improving photosynthetic leaf pigment contents and the uptake of nutrition. Compared with C. etunicatum, R. intraradices showed a stronger tolerance to Mo, which was manifested by a stronger inhibition of Mo transport and a higher uptake of nutrient elements. Accordingly, AMF show potential for the bioremediation of Mo-polluted soil. [ABSTRACT FROM AUTHOR]

Metal–organic frameworks (MOFs) take on highly ordered self-assembled structures and have gained much attention due to their intriguing structural features and enormous applications. Typical features of MOFs include large surface areas, nanoporosity, tunable size and shape, and reliable host–guest interactions, making them demanding candidates in sensor applications. Moreover, MOFs offer an opportunity to upgrade selectivity toward certain analytes in response to various chemical and physical stimulations. In this review, we have elaborated on the structural features of MOFs for biological sensing and the MOF-analyte interaction originating from the inherent porosity, vacancy defects, or abundance functional groups. Also, we summarize the state-of-the-art progress on MOF-based sensors applied in healthcare, food safety, environmental monitoring, and pharmaceutics, category by transduction mechanisms involving optical, electrochemical, and plasmons. In addition, we have discussed in detail the possible applications of MOF-based material for identifying high performance and detecting food freshness, industrial gas leakage, and medical diagnostics. Finally, current limitations regarding sensing performance in practical applications are also overviewed. Thus, this literature review will open new doors for chemists or bioengineers to tune the structural features further to modulate the sensing ability of low-cost materials for biosensing applications in future endeavors. [ABSTRACT FROM AUTHOR]

Arbuscular mycorrhiza (AM) and ectomycorrhiza (ECM) are the two most common mycorrhizal types and are paid the most attention to, playing a vital common but differentiated function in terrestrial ecosystems. The leaf carbon isotope ratio (δ13C) is an important factor in understanding the relationship between plants and the environment. In this study, a new database was established on leaf δ13C between AM and ECM plants based on the published data set of leaf δ13C in China's C3 terrestrial plants, which involved 1163 observations. The results showed that the differences in leaf δ13C between AM and ECM plants related closely to life forms. Leaf δ13C of ECM plants was higher than that of AM plants in trees, which was mainly led by the group of evergreen trees. The responses of leaf δ13C to environmental changes were varied between AM and ECM plants. Among the four life forms, leaf δ13C of ECM plants decreased more rapidly than that of AM plants, with an increase of longitude, except for deciduous trees. In terms of the sensitivity of leaf δ13C to temperature changes, AM plants were higher than ECM plants in the other three life forms, although there was no significant difference in evergreen trees. For the response to water conditions, the leaf δ13C of ECM plants was more sensitive than that of AM plants in all life forms, except evergreen and deciduous trees. This study laid a foundation for further understanding the role of mycorrhiza in the relationship between plants and the environment. [ABSTRACT FROM AUTHOR]

Nitrogen (N) deposition is known to significantly affect plant growth. Mycorrhizas play an important role in plant productivity, and plants of different mycorrhizal types respond differently to global change, which will inevitably affect plant response to N deposition. However, little is known about the differences of different mycorrhizas in biomass allocation of host plants in response to N addition. Here, a meta-analysis of data from N addition experiments was carried out to analyze the response of biomass in arbuscular mycorrhiza (AM) and ectomycorrhiza (ECM) plants to N addition. The results showed that biomass of leaf, stem, fine root (FR), and litter between AM and ECM plants responded differently to N addition (p < 0.05). Among them, biomass of leaf and stem in ECM plants (leaf: 46.89%; stem: 45.59%) was more sensitive (positively) to N addition than AM plants (leaf: 27.84%; stem: 10.30%) (p < 0.05). N addition suppressed biomass of FR in AM plants (−11.22%) but promoted that in ECM plants (13.77%). The effects on biomass also varied with different functional groups between AM and ECM plants. However, the N responses were influenced by other resources. When other treatments were added, biomass was less varied in AM plants compared to ECM plants. In addition, the N response of WB (whole biomass) and root biomass were positively correlated with annual temperature in ECM plants, but that in AM plants did not. The effects on shoot biomass in AM and ECM plants to N addition both decreased with annual temperature. The N response of root biomass increased with annual precipitation. It can be seen that different mycorrhizal types regulate the response of different plant organ biomass to N addition, which is significant for predicting ecosystem responses and feedback to environmental change. [ABSTRACT FROM AUTHOR]

Leaf nitrogen (N) and phosphorus (P) are the most important functional traits in plants which affect biogeochemical cycles. As the most widely observed plant–fungus mutualistic symbiosis, mycorrhiza plays a vital role in regulating plant growth. There are different types of mycorrhiza with various ecological functions in nature. Drought, as a frequent environmental stress, has been paid more and more attention due to its influence on plant growth. Numerous studies have confirmed that drought affects the concentration of N and P in plants, but few studies involve different mycorrhizal types of plants. In this study, the differences of N and P between arbuscular mycorrhizal (AM) and ectomycorrhizal (ECM) plants under different drought patterns, drought duration and cultivation conditions were explored based on a dataset by a meta-analysis. Drought stress (DS) showed negative effects on AM plant N (−7.15%) and AM plant P (−13.87%), and a positive effect on AM plant N:P ratio (+8.01%). Drought significantly increased N and the N:P ratio of ECM plants by 1.58% and 3.58%, respectively, and decreased P of ECM plants by −2.00%. Short-term drought (<30 d) reduces more N and P than long-term drought (<30 d) in AM plant species. The duration of drought did not change the N concentration of ECM plant N, while short-term drought reduced ECM plant P. The effects of N and P on DS also varied with different planting conditions and functional groups between AM and ECM plants. Therefore, mycorrhizal effects and stoichiometry of N and P play a key role in plant response to drought. So mycorrhizal effects should be considered when studying plant responses to drought stress. [ABSTRACT FROM AUTHOR]

(1) Background: environmental gradient strongly affects microbial biodiversity, but which factors drive the diversity of arbuscular mycorrhizal fungi (AMF) associated with roots at relatively large spatial scales requires further research; (2) Methods: an experiment on large spatial scales of Mt. Taibai was conducted to explore the biodiversity and drivers of AMF-associated with roots using high-throughput sequencing; (3) Results: a total of 287 operational taxonomic units (OTUs) belong to 62 species representing 4 identified and 1 unclassified order were identified along different altitudinal gradients. With increasing altitude, AMF colonization could be simulated by a quadratic function trend, and altitude has a significant impact on colonization. AMF alpha diversity, including the Sobs and Shannon indexes, tended to be quadratic function trends with increasing altitude. The highest diversity indices occurred at mid-altitudes, and altitude had a significant effect on them. AMF communities have different affinities with soil and root nutrient, and Glomus is most affected by soil and root nutrient factors through the analysis of the heatmap. Glomus are the most dominant, with an occurrence frequency of 91.67% and a relative abundance of 61.29% and 53.58% at the level of species and OTU, respectively. Furthermore, AMF diversity were mostly associated with soil and root nutrients; (4) Conclusions: in general, AMF molecular diversity is abundant in Mt. Taibai, and altitude and nutrient properties of soil and root are the main influencing factors on AMF diversity and distribution. [ABSTRACT FROM AUTHOR]

(1) Background: Understanding the diversity of communities of arbuscular mycorrhizal fungi (AMF) is the basis for understanding the ecological functions of AMF. (2) Methods: The community diversity and distribution of AMF at different elevations in tropical montane rainforests of Mt. Jianfenfling and Mt. Diaoluo were explored using high-throughput sequencing technology. (3) Results: A total of 283 AMF operational taxonomic units (OTUs) were identified from roots and the number of unique OTUs was 173, accounting for 61.13% of the total number discovered in these tropical montane rainforests. At different altitudes, high turnovers of AMF were observed, with the maximum proportion of unique OTUs between two altitudes being 45.16%, recorded between a.s.l. 250 m and 900 m on Mt. Diaoluo. The highest Sobs, Shannon and Pielou diversity indices appeared at 650 m on Mt. Diaoluo. For the two mountains, the soil properties of C, N and C/N have significant impacts on the genera Scutellospora, Paraglomus and unclassified in Archaeosporaceae, while the genera Glomus, Diversispora and Acaulospora are significantly affected by soil P and pH. It can be considered that altitude probably determines the presence of AMF communities by affecting edaphic properties. (4) Conclusions: There are abundant AMF associated with roots in the tropical montane rainforests of China. Furthermore, a high turnover of OTUs was found to exist between the mountains and at different altitudes, revealing diverse AMF community structures in tropical montane rainforests. [ABSTRACT FROM AUTHOR]

Daptomycin (DAP), a last-resort antibiotic for treating Gram-positive bacterial infection, has been widely used in the treatment of vancomycin-resistant enterococci (VRE). Resistance to both daptomycin and vancomycin leads to difficulties in controlling infections of enterococci. A clinical multidrug-resistant Enterococcus faecium EF332 strain that shows resistance to both daptomycin and vancomycin was identified, for which resistance mechanisms were investigated in this work. Whole-genome sequencing and comparative genomic analysis were performed by third-generation PacBio sequencing, showing that E. faecium EF332 contains four plasmids, including a new multidrug-resistant pEF332-2 plasmid. Two vancomycin resistance-conferring gene clusters vanA and vanM were found on this plasmid, making it the second reported vancomycin-resistant plasmid containing both clusters. New mutations in chromosomal genes cls and gdpD that, respectively, encode cardiolipin synthase and glycerophosphoryl diester phosphodiesterase were identified. Their potential roles in leading to daptomycin resistance were further investigated. Through molecular cloning and phenotypic screening, two-dimensional thin-layer chromatography, fluorescence surface charge test, and analysis of cardiolipin distribution patterns, we found that mutations in cls decrease surface negative charges of the cell membrane (CM) and led to redistribution of lipids of CM. Both events contribute to the DAP resistance of E. faecium EF332. Mutation in gdpD leads to changes in CM phospholipid compositions, but cannot confer DAP resistance. Neither mutation could result in changes in cellular septa. Therefore, we conclude that the daptomycin resistance of E. faecium EF332 is conferred by new cls mutations. This work reports the genetic basis for vancomycin and daptomycin resistance of a multidrug-resistant E. faecium strain, with the finding of new mutations of cls that leads to daptomycin resistance. [ABSTRACT FROM AUTHOR]

With the wide clinical use of the third‐generation epidermal growth factor receptor (EGFR) inhibitor osimertinib for the treatment of EGFR‐mutated non–small cell lung cancer (NSCLC), acquired resistance caused by EGFR C797S tertiary mutation has become a concern. Therefore, fourth‐generation EGFR inhibitors that could overcome this mutation have gained increasing attention in recent years. Here, we identified LS‐106 as a novel EGFR inhibitor against C797S mutation and evaluated its antitumor activity both in vitro and in vivo. In cell‐free assay, LS‐106 potently inhibited the kinase activities of EGFR19del/T790M/C797S and EGFRL858R/T790M/C797S with IC50 values of 2.4 nmol/L and 3.1 nmol/L, respectively, which was more potent than osimertinib. Meanwhile, LS‐106 exhibited comparable kinase inhibitory effect to osimertinib on EGFRL858R/T790M and wild‐type EGFR. Results from cellular experiments demonstrated that LS‐106 potently blocked the phosphorylation of EGFR C797S triple mutations in the constructed BaF3 cells that highly expressed EGFR19del/T790M/C797S or EGFRL858R/T790M/C797S, and thus inhibited the proliferation of these cells. We also constructed tumor cells harboring EGFR19del/T790M/C797S (named PC‐9‐OR cells) using the CRISPR/Cas9 system and found that LS‐106 markedly suppressed the activation of EGFR19del/T790M/C797S and the proliferation of PC‐9‐OR cells. Moreover, cells harboring EGFR19del/T790M/C797S underwent remarkable apoptosis upon LS‐106 treatment. In vivo experiments further demonstrated that oral administration of LS‐106 caused significant tumor regression in a PC‐9‐OR xenograft model, with a tumor growth inhibition rate (TGI) of 83.5% and 136.6% at doses of 30 and 60 mg/kg, respectively. Taken together, we identified LS‐106 as a novel fourth‐generation EGFR inhibitor against C797S mutation and confirmed its preclinical antitumor effects in C797S–triple‐mutant tumor models. [ABSTRACT FROM AUTHOR]

The sizable prevalence of autism spectrum disorder (ASD) in China has recently emerged as an urgent public health issue; however, studies investigating parenting stress in Chinese parents of children with ASD lack clarity in identifying the potential factors of stress. The aim of this systematic review was to identify and categorize the potential factors of stress based on findings in the literature. An extensive search of articles in online databases, including PubMed, Web of Science, and EBSCOhost (ERIC, MEDLINE and PsycINFO), was conducted, as a result of which 24 eligible articles published from 2007 to 2019 were identified. Four key categories of potential factors of parental stress—cultural factors, parents' psychopathological symptoms, problem behaviors in ASD children, and caregiver burden—were identified. The impact of these potential factors on parental coping strategies and on the quality of life in affected families were also discussed. [ABSTRACT FROM AUTHOR]