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Your search keyword '"Zhang, Jianfei"' showing total 541 results
Start Over Author "Zhang, Jianfei" Publication Type Academic Journals
541 results on '"Zhang, Jianfei"'

5. Negative mixing enthalpy solid solutions deliver high strength and ductility

Author

An, Zibing, Li, Ang, Mao, Shengcheng, Yang, Tao, Zhu, Lingyu, Wang, Rui, Wu, Zhaoxuan, Zhang, Bin, Shao, Ruiwen, Jiang, Cheng, Cao, Boxuan, Shi, Caijuan, Ren, Yang, Liu, Cheng, Long, Haibo, Zhang, Jianfei, Li, Wei, He, Feng, Sun, Ligang, Zhao, Junbo, Yang, Luyan, Zhou, Xiaoyuan, Wei, Xiao, Chen, Yunmin, Lu, Zhouguang, Ren, Fuzeng, Liu, Chain-Tsuan, Zhang, Ze, and Han, Xiaodong

Published
2024
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31. Identification of differentially expressed proteins involved in fetal scarless wound healing using a rat model of cleft lip

Author

Yan, Yu, Liu, Hong, Yi, Jiarong, Chen, Zizi, Chen, Jia, Zhang, Jianfei, Gao, Kewa, He, Siqi, Wang, Aijun, Jin, Ping, Hu, Feng, and Zhou, Jianda

Subjects
Medicinal and Biomolecular Chemistry, Chemical Sciences, Biotechnology, Pediatric, Genetics, Reproductive health and childbirth, Animals, Cicatrix, Cleft Lip, Female, Fetus, Gene Expression, Lip, Pregnancy, Proteomics, RNA, Messenger, Rats, Rats, Sprague-Dawley, Wound Healing, cleft lip, scarless healing, label-free quantification, proteomics, parallel reaction monitoring, label‑free quantification, Biochemistry and Cell Biology, Oncology & Carcinogenesis, Medicinal and biomolecular chemistry
Abstract

In early pregnancy, fetal skin wounds can heal quickly and undergo a transition period from scarless healing to scar formation. The aim of the present study was to identify potential biomarkers associated with scarless repair of cleft lips, in order to determine the intrinsic factors leading to scar formation in embryonic tissue. A stable model of cleft lip was established using microsurgery by constructing a wedge‑shaped cleft lip‑like defect in fetal rats at gestational age (GA) 16.5 and GA18.5. The GA16.5 and GA18.5 groups were used to model scarless healing and scar formation, respectively. The fetuses were returned to the uterus following surgery, then removed 72 h after the procedure. Macroscopic observation of the cleft defect and histological examination were carried out. Reverse transcription‑quantitative (RT‑q) PCR and parallel reaction monitoring (PRM) were used to detect mRNA and protein expression levels, respectively. The upper‑left lip completely healed 72 h after surgery in the GA16.5 group of fetal rats. However, this was not the case in the GA18.5 group. Histological examination indicated new follicles visible under the epidermis of the scarless group (GA16.5). Scarring was visible on the upper‑left cleft lip wound of the fetal rats in the GA18.5 group. The expression of some growth and pro‑inflammatory factors, including TNF‑α, were also different between two groups. Label‑free quantification was used to identified differentially expressed proteins and five differentially expressed proteins (Smad4, Fabp5, S100a4, S100a8 and S100a9) were identified. The relative expression of these molecules at the mRNA and protein levels were measured using RT‑qPCR and PRM. These molecules may represent potential biomarkers for the scarless repair of fetal rat cleft lip wounds.

Published
2021

33. Research progress in hydrogel microneedle

Author

LIU Guodong, LI Zheng, HAO Limin, GONG Jixian, ZHANG Jianfei, and ZHANG Yongjun

Subjects
microneedle, hydrogel, micro-molding, drug delivery, interstitial fluid extraction, Materials of engineering and construction. Mechanics of materials, TA401-492
Abstract

Microneedles (MN) as a minimally invasive device consisting of a micro-raised array, can penetrate the cuticle to the epidermis and dermis, and which has the advantages of safety, painless, minimally invasive, self-administration and convenience. As a new kind of microneedles, hydrogel microneedles have attracted more attentions in the medical field due to its excellent performance. Hydrogel microneedles have good biocompatibility and mechanical properties, and can be completely removed after skin action without residual polymer in the body. Its unique swelling property can realize minimally invasive extraction of human detection substance and slow release of drugs, which can play a huge role in the field of personal health monitoring and drug controlled release in the future. The mechanism of action, design, preparation and application of hydrogel microneedles were reviewed in this paper, focusing on the current design parameters of hydrogel microneedles and their applications in drug delivery, extraction monitoring and wound healing, and the problems of hydrogel microneedles in skin infection risk, pharmacokinetics and wearing comfort were pointed out. In the future, the key research direction is to combine with intelligent devices to realize both human body monitoring and intelligent drug controlled release on the microneedle patch.

Published
2023
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46. Unraveling the pathogenic potential of the Pentatrichomonas hominis PHGD strain: impact on IPEC-J2 cell growth, adhesion, and gene expression

Author

Zhu Yibin, Cai Haiming, Fang Siyun, Shen Hanqin, Yan Zhuanqiang, Wang Dingai, Qi Nanshan, Li Juan, Lv Minna, Lin Xuhui, Hu Junjing, Song Yongle, Chen Xiangjie, Yin Lijun, Zhang Jianfei, Liao Shenquan, and Sun Mingfei

Subjects
pentatrichomonas hominis, ipec-j2, cell viability, inflammatory response, Infectious and parasitic diseases, RC109-216
Abstract

Pentatrichomonas hominis, a flagellated parasitic protozoan, predominantly infects the mammalian digestive tract, often causing symptoms such as abdominal pain and diarrhea. However, studies investigating its pathogenicity are limited, and the mechanisms underlying P. hominis-induced diarrhea remain unclear. Establishing an in vitro cell model for P. hominis infection is imperative. This study investigated the interaction between P. hominis and IPEC-J2 cells and its impact on parasite growth, adhesion, morphology, and cell viability. Co-cultivation of P. hominis with IPEC-J2 cells resulted in exponential growth of the parasite, with peak densities reaching approximately 4.8 × 105 cells/mL and 1.2 × 106 cells/mL at 48 h for initial inoculation concentrations of 104 cells/mL and 105 cells/mL, respectively. The adhesion rate of P. hominis to IPEC-J2 cells reached a maximum of 93.82% and 86.57% at 24 h for initial inoculation concentrations of 104 cells/mL and 105 cells/mL, respectively. Morphological changes in IPEC-J2 cells co-cultivated with P. hominis were observed, manifesting as elongated and irregular shapes. The viability of IPEC-J2 cells exhibited a decreasing trend with increasing P. hominis concentration and co-cultivation time. Additionally, the mRNA expression levels of IL-6, IL-8, and TNF-α were upregulated, whereas those of CAT and CuZn-SOD were downregulated. These findings provide quantitative evidence that P. hominis can promote its growth by adhering to IPEC-J2 cells, inducing morphological changes, reducing cell viability, and triggering inflammatory responses. Further in vivo studies are warranted to confirm these results and enhance our understanding of P. hominis infection.

Published
2024
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