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Abstract The automotive industry aims to ensure the cold-start capability of polymer electrolyte fuel cells (PEFCs) for developing fuel cell electric vehicles that can be driven in cold climates. Water and ice behavior is a key factor in maintaining this capability. Previously reported methods for visualizing water and/or ice are limited to small-sized PEFCs (

Effective government programmes should be taken up by their potential beneficiaries. This study investigates the factors associated with the decision of Vietnamese small business owners to participate in free business training programmes. Based on the original survey data, I find that most business owners were unaware of the importance of learning business, and thus, did not take up the training offer. The regression analysis, using bivariate probit model, shows that the risk-taking owners and owners with their relatives also invited to the same training were more likely to participate in the training. These findings suggest that providing ex-ante information about the usefulness of the training and allowing group participation can encourage training participation.

The rice endosperm protein (REP) hydrolysate containing the following rice endosperm protein derived oligopeptides QQFLPEGQSQSQK, LPEGQSQSQK, and pEQFLPEGQSQSQK (a N-terminal pyroglutamate residue-modified peptide) reportedly showed an antidepressant-like effect in an animal model. We investigated the effect of the REP hydrolysate on healthy humans who self-reported mental fatigue with subjectively low vigor. Seventy-six participants (age: 20–64 years) were randomly allocated to two groups. The influence of the REP hydrolysate on the mood state was evaluated in two studies: single intake (Study 1) and repeated intake over 4 weeks (Study 2). A salivary stress marker, Chromogranin A (CgA), was measured in Study 1. The single intake of the REP hydrolysate significantly improved the Profile of Mood Status 2nd edition for adults (POMS 2) subscale of Tension–Anxiety. Additionally, the salivary CgA concentrations were remarkably reduced after the single intake of the REP hydrolysate. Though a single intake of the REP hydrolysate did not significantly influence the other subscales and the TMD of the POMS 2 and the Euthymia Scale, both the subjective and objective results supported the possible effect of the REP hydrolysate on reducing anxiety and nervousness. No significant positive effects on the subjective mood state (Euthymia Scale and POMS 2) and sleep quality (Insomnia Severity Index) were observed in the trial setting employed for Study 2. In conclusion, a single intake of REP hydrolysate might help relax the subjective feelings of tension and anxiety. The effectiveness of repeated REP hydrolysate intake needs to be tested in a different clinical setting.

Brain serotonin (5-hydroxytryptamine, 5-HT) is a key molecule for the mediation of depression-related brain states, but the neural mechanisms underlying 5-HT mediation need further investigation. A possible mechanism of the therapeutic antidepressant effects is neurogenic cell production, as stimulated by 5-HT signaling. Neurogenesis, the proliferation of neural stem cells (NSCs), and cell differentiation and maturation occur across brain regions, particularly the hippocampal dentate gyrus and the subventricular zone, throughout one's lifespan. 5-HT plays a major role in the mediation of neurogenic processes, which in turn leads to the therapeutic effect on depression-related states. In this review article, we aim to identify how the neuronal 5-HT system mediates the process of neurogenesis, including cell proliferation, cell-type differentiation and maturation. First, we will provide an overview of the neurogenic cell transformation that occurs in brain regions containing or lacking NSCs. Second, we will review brain region-specific mechanisms of 5-HT-mediated neurogenesis by comparing regions localized to NSCs, i.e., the hippocampus and subventricular zone, with those not containing NSCs. Highlighting these 5-HT mechanisms that mediate neurogenic cell production processes in a brain-region-specific manner would provide unique insights into the role of 5-HT in neurogenesis and its associated effects on depression.

We conducted a randomized experiment targeting 322 Japanese high school students to examine the impacts of a newly developed English-language learning program. The treated students were offered an opportunity to communicate for 25 minutes with English-speaking Filipino teachers via Skype several times a week over a 5-month period as an extracurricular activity. The results show that the Skype program increased the interest of the treated students in an international vocation and in foreign affairs. However, the students did not improve their English communication abilities, as measured by standardized tests, probably because of the program's low utilization rate. Further investigation showed that the utilization rate was particularly low among students demonstrating a tendency to procrastinate. These results suggest the importance of maintaining students’ motivation to keep using such information and communication technology-assisted learning programs if they are not already incorporated into the existing curriculum. Having procrastinators self-regulate may be especially crucial.

Though danger prediction and countermeasures for landslides are important, it is fundamentally difficult to take preventive measures in all areas susceptible to dangerous landslides. Therefore, it is necessary to perform landslide susceptibility mapping, extract slopes with high landslide hazard/risk, and prioritize locations for conducting investigations and countermeasures. In this study, landslide susceptibility mapping along the whole slope of the Japanese archipelago was performed using the analytical hierarchy process (AHP) method, and geographic information system analysis was conducted to extract the slope that had the same level of hazard/risk as areas where landslides occurred in the past, based on the ancient landslide topography in the Japanese archipelago. The evaluation factors used were elevation, slope angle, slope type, flow accumulation, geology, and vegetation. The landslide susceptibility of the slope was evaluated using the score accumulation from the AHP method for these evaluation factors. Based on the landslide susceptibility level (I to V), a landslide susceptibility map was prepared, and landslide susceptibility assessment in the Japanese archipelago was identified. The obtained landslide susceptibility map showed good correspondence with the landslide distribution, and correlated well with past landslide occurrences. This suggests that our method can be applied to the extraction of unstable slopes, and is effective for prioritizing and implementing preventative measures.

In recent years, severe competition is executed on getting air cargos. The forecast of the number of taking-off and landing is expanding. Strict marketing is required in such fields. Forecasting the trend of air cargo is an essential item to be investigated in airlines. In order to make forecast for time series, the method of using linear model is often used. Forecasting using neural network is also developed. Reviewing past researches, there are many researches made on this. There is many room to improve in neural network, therefore we make focus on them. We use time series data, and in order to make forecast, a new coming data should be handled and the parameter should be estimated based upon its data. This is a so-called on-line parameter estimation. In this paper, neural network is applied and Multilayer perceptron Algorithm is newly developed. The method is applied to the Airlines Cargo Data in the case of Daily data. When there is a big change of the data, the neural networks cannot learn the past data properly, therefore we have devised a new method to cope with this. Repeating the data into plural section, smooth change is established and we could make a neural network learn more smoothly. The result is compared with the method of ARIMA model. The forecasting results are measured by the Forecasting Accuracy Ratio which is the measure of the normalized residual part of the forecasting error. Good results were obtained. The new method shows that it is useful for the time series that has various trend characteristics and has rather strong seasonal trend. The effectiveness of this method should be examined in various cases.

Stress induces various neurobiological responses and causes psychiatric disorders, including depression. Monoamine oxidase A (MAO-A) plays an important role in various functions of the brain, such as regulation of mood, anxiety and aggression, and dysregulation of MAO-A is observed in stress-related psychiatric disorders. This study addressed the question whether acute social stress induces changes to transcriptional and/or post-transcriptional regulation of MAO-A expression in the brain. Using male Nile tilapia (Oreochromis niloticus), we investigated whether acute social stress, induced by the presence of a dominant male fish, changes the expression of MAO-A. We measured gene expression of MAO-A by quantitative PCR, enzymatic activity of MAO-A by the luminescent method, and 5-HT and 5-HIAA levels by liquid chromatography–mass spectrometry in the brain of socially stressed and control fish. Socially stressed males showed decreased MAO-A mRNA levels, consistent MAO-A enzymatic activity, increased 5-HT turnover in the brain, and elevated plasma cortisol levels, compared to controls. Our results suggest that acute social stress suppresses the transcription of MAO-A gene, enhances 5-HT metabolism but does not affect the production of MAO-A protein.

Monoamine oxidase A (MAO-A) is an enzyme that regulates the levels of monoamine neurotransmitters, such as serotonin, noradrenaline and dopamine and it has been used as a therapeutic target for depression. However, MAO-A inhibitors, which directly acts on MAO-A protein, have limited use due to their adverse effects. microRNAs (miRNAs) are 18–22 nucleotide long, small non-coding RNAs, which have recently emerged as regulators of protein levels that could potentially be new therapeutic targets for psychiatric disorders. This review article aims to discuss the current status of the treatment for depression with MAO-A inhibitors and the regulatory factors of MAO-A. Further, the review also proposes possible regulatory mechanisms of MAO-A by miRNAs, which leads to better understanding of the pathology of depressive disorders and their potential use as therapeutic agents.

Social stress has a high impact on many biological systems in the brain, including serotonergic (5-HT) system—a major drug target in the current treatment for depression. Hyperactivity of hypothalamic-pituitary-adrenal (HPA) axis and monoamine oxidase A (MAO-A) are well-known stress responses, which are involved in the central 5-HT system. Although, many MAO-A inhibitors have been developed and used in the therapeutics of depression, effective management of depression by modulating the activity of MAO-A has not been achieved. Identifying the molecular pathways that regulate the activity of MAO-A in the brain is crucial for developing new drug targets for precise control of MAO-A activity. Over the last few decades, several regulatory pathways of MAO-A consisting of Kruppel like factor 11 (KLF11), Sirtuin1, Ring finger protein in neural stem cells (RINES), and Cell division cycle associated 7-like protein (R1) have been identified, and the influence of social stress on these regulatory factors evaluated. This review explores various aspects of these pathways to expand our understanding of the roles of the HPA axis and MAO-A regulatory pathways during social stress. The first part of this review introduces some components of the HPA axis, explains how stress affects them and how they interact with the 5-HT system in the brain. The second part summarizes the novel regulatory pathways of MAO-A, which have high potential as novel therapeutic targets for depression.

Background: Lacticaseibacillus paracasei 327 (L. paracasei 327) is a lactic acid bacteria isolated from brown rice. It has been reported that sterilized L. paracasei 327 improves bowel movement. Given the reported relationship between intestinal microbiota and bowel movement, we hypothesized that L. paracasei 327 also improves the intestinal environment through its involvement in fluctuations in the composition of the intestinal microbiota. Objective: The purpose of this study was to verify the effects of the consumption of food containing sterilized L. paracasei 327 on the intestinal environment in healthy Japanese adults. Methods: A randomized, double-blind, placebo-controlled, parallel-group trial was conducted in 110 healthy adults aged 20 to 64 years with a defecation frequency of three to five times per week. Participants were divided into two groups, one receiving 25 mg (approximately 5x1010 bacteria) of sterile L. paracasei 327 once daily for two weeks (55 participants) and other receiving placebo for two weeks (55 participants), to test its effect on the intestinal environment. Results: Intestinal microbiota analysis showed that L. paracasei 327 ingestion resulted in a trend towards an increase in Bacteroides and a significant decrease in Clostridium cluster IV in each taxonomic group compared to the placebo group. In addition, for each operational taxonomic units (OTUs), Bacteroides_OTU_469 and Clostridium subcluster XIVa_OTU_754 were significantly increased, and Clostridium cluster IX, Akkermansia_OTU_110 tended to increase, while Clostridium cluster IV_OTU_749 significantly decreased. Furthermore, defecation days and defecation frequency increased significantly. Conclusion: The presumptive species of bacteria in the taxonomic group and OTUs increased by L. paracasei 327 ingestion included short-chain fatty acid-producing bacteria. In addition, Clostridium cluster IV_OTU_749, which was reduced by L. paracasei 327 ingestion, may also be involved in the production of putrefactive products in the intestine. Therefore, these findings suggest that L. paracasei 327 ingestion improves the intestinal environment by increasing short-chain fatty acidproducing bacteria, suppressing putrefactive-producing bacteria, and improving defecation. [ABSTRACT FROM AUTHOR]

Efficient biomarkers for diabetic nephropathy (DN) have not been established. Using ELISA, we found previously that urinary levels of full-length megalin (C-megalin), a multiligand endocytic receptor in proximal tubules, was positively correlated with DN progression in patients with type 2 diabetes mellitus (T2DM). Here, we found that urinary extracellular vesicle (UEV) excretion and C-megalin content in UEVs or in their exosomal fraction increased along with the progression of the albuminuric stages in patients with T2DM. Cultured immortalized rat proximal tubule cells (IRPTCs) treated with fatty acid-free BSA or advanced glycation end product-modified BSA (AGE-BSA), endocytic ligands of megalin, increased EV excretion, and their C-megalin content. C-megalin excretion from IRPTCs via extracellular vesicles was significantly blocked by an exosome-specific inhibitor, GW4869, indicating that this excretion is mainly exocytosis-mediated. AGE-BSA treatment of IRPTCs caused apparent lysosomal dysfunction, which stimulated multivesicular body formation, resulting in increased exosomal C-megalin excretion. In a high-fat diet-induced, megalin-mediated kidney injury model in mice, urinary C-megalin excretion also increased via UEVs. Collectively, exocytosis-mediated urinary C-megalin excretion is associated with the development and progression of DN in patients with T2DM, particularly due to megalin-mediated lysosomal dysfunction in proximal tubules, and hence it could be a candidate biomarker linked with DN pathogenesis. [ABSTRACT FROM AUTHOR]

In industries, making a correct forecasting is inevitable. There are many researches made on this. In this paper, a hybrid method is introduced and plural methods are compared. Focusing that the equation of exponential smoothing method (ESM) is equivalent to (1, 1) order ARMA model equation, a new method of estimation of smoothing constant in exponential smoothing method is proposed before by us which satisfies minimum variance of forecasting error. Combining the trend removing method with this method, we aim to improve forecasting accuracy. Trend removing by the combination of linear and 2nd order non-linear function and 3rd order non-linear function is executed to the original production data of two kinds of prepared frozen fry foods. Genetic Algorithm is utilized to search optimal weights for the weighting parameters of linear and non-linear function. For the comparison, monthly trend is removed after that. Theoretical solution of smoothing constant of ESM is calculated. Then forecasting is executed on these data. In particular, Mahalanobis' generalized distance without weight is proposed as for one of the method to measure the forecasting accuracy. The new method shows that it is useful for the time series that has various trend characteristics and has rather strong seasonal trend. [ABSTRACT FROM AUTHOR]

In the presence of TMSSnBu3and CsF, stannylation of N-Boc- and N-Cbz-α-amido sulfones proceeded very well to afford the corresponding α-amido stannanes in moderate-to-high yields. This reaction tolerated α-aryl-, alkenyl-, and alkyl-substituted α-amido sulfones as well as substrates containing either an ester or cyano moiety, which might be reactive with lithium or magnesium stannides employed in conventional stannylation. [ABSTRACT FROM AUTHOR]

Arylative carboxylation of allenes proceeded in an intramolecular manner to afford the corresponding β,γ-unsaturated carboxylic acids in high yields using PdCl2/PAr3 (Ar = C6H4-p-CF3) and ZnEt2 under 1 atm of CO2. The intermediate of the cyclization/carboxylation sequence is thought to be a nucleophilic η¹-allylethylpalladium, which reacts with CO2 at the γ-position of palladium. The products obtained could be efficiently converted into 3-substituted indole-2-carboxylate derivatives. One-pot synthesis of strychnocarpine, a β-carboline alkaloid, from the carboxylated product was also demonstrated. [ABSTRACT FROM AUTHOR]

In order to study the variation of a microscopic internal magnetic field with the Ca content (x), we have systematically measured muon-spin rotation and relaxation (μ+SR) spectra for Sr1-xCaxCo2P2 (0 ⩽ x ⩽ 1) powder samples mainly in a zero external field. As x increases from 0, a Pauli-paramagnetic phase is observed even at the lowest T measured (1.8 K) until x = 0.45; then, a short-range anti ferromagnetic (AF) ordered phase appears for 0.48 ⩽ x ⩽0.75, and finally a long-range AF ordered phase is stabilized for x ⩾ 0.75. The evolution of the magnetic order is connected to the shrinking of the c-axis length as a function of x, which naturally enhances the AF interaction between the two adjacent Co planes. [ABSTRACT FROM AUTHOR]

To investigate the microscopic magnetic nature of phospho-olivines, we have measured positive muon-spin rotation and relaxation (μ+SR) spectra for NaxFePO4 and FePO4, using powder samples in the temperature range between 2 and 200 K. For Na0.7FePO4, μ+SR measurements under zero external field (ZF) showed the appearance of static magnetic order below TN ~ 61 K, at which the susceptibility Χ(T) exhibits a sharp maximum due to an antiferromagnetic (AF) transition. The wide distribution found for the internal magnetic field (Hint) was reasonably explained by the formation of AF order with the spread in Hint due to a random distribution of Na vacancies. On the other hand, the ZF spectra for FePO4 suggested the formation of the canted AF order below TN = 113 K, as proposed by neutron diffraction (ND), but the ratio between the y and x components of the ordered moment (My/Mx) was determined as 0.6 by μ+SR, whereas 0.22 was measured by ND, probably indicating anisotropic spin fluctuations in FePO4. [ABSTRACT FROM AUTHOR]