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Immune checkpoint blockade (ICB) targeting PD-1/PD-L1 has been used for the treatment of head and neck squamous cell carcinoma (HNSCC). However, the overall response rate to ICB therapy for HNSCC remains less than 20%. It has recently been reported that the appearance of tertiary lymphoid structures (TLSs) in tumor tissue is correlated with better prognosis and response to ICB treatment. Here, we demonstrated an immune classification for the tumor microenvironment (TME) of HNSCC by analyzing The Cancer Genome Atlas (TCGA)–HNSCC data set and found that immunotype D with TLS enrichment had a better prognosis and response to ICB treatment. Furthermore, we observed that TLSs were present in a part of tumor samples of human papillomavirus (HPV) infection negative HNSCC (HPV− HNSCC) and were associated with the densities of dendritic cell (DC)–LAMP+ DCs, CD4+ T cells, CD8+ T cells, and progenitor T cells in TME. We established an HPV− HNSCC mouse model with TLS-enriched TME by overexpressing LIGHT in a mouse HNSCC cell line. We found that the induction of TLS formation enhanced the response to PD-1 blockade treatment in the HPV− HNSCC mouse model, accompanied by increases in DCs and progenitor exhausted CD8+ T cells in the TME. Elimination of CD20+ B cells attenuated the therapeutic effect of PD-1 pathway blockade in TLS+ HPV− HNSCC mouse models. These results indicate that TLSs contribute to the favorable prognosis and antitumor immunity of HPV− HNSCC. Inducing TLS formation in HPV− HNSCC tumors is a potential therapeutic method for improving the ICB response rate in patients with HPV− HNSCC. [ABSTRACT FROM AUTHOR]

Most species of the family Cupressaceae are widely distributed throughout China, but Sabina przewalskii f. pendula and Juniperus przewalskii are endemic to the Qinghai-Tibet Plateau region. Therefore, they are of great significance to the plateau ecosystem. Sabina przewalskii and J. przewalskii have similar characteristics, but they be distinguished by the presence or absence of drooping branchlets. However, this feature can be difficult to distinguish with the naked eye. In this study, the complete chloroplast (CP) genomes of S. przewalskii and J. przewalskii were determined by highthroughput sequencing, aiming to unveil their differences at the molecular level. The sizes of the CP genomes of J. przewalskii and S. przewalskii are 128,748 bp and 127,315 bp, respectively, sharing the typical structure of conifer species. The GC content of the S. przewalskii plastome is 35.08%, encoding 84 proteins, 32 tRNAs and 4 rRNAs. The GC content of the J. przewalskii CP genome is 34.98%, and one additional rpl32 gene was detected. Phylogenetic analysis proved that J. przewalskii showed a close relationship to Juniperus tibetica and S. przewalskii, both of which belong to the family Cupressaceae. Our results of the plastome of J. przewalskii and S. przewalskii further clarify the evolution of Cupressaceae species. The divergent regions and the repetitive sequences identified could be developed as molecular markers that would benefit breeding and species discrimination. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To explore the effect of polyethylene glycol loxenatide (long-acting GLP-1R agonist) on the lipid, glucose levels, and weight in type 2 diabetes mellitus patients with obesity. PATIENTS AND METHODS: A total of 40 obese patients with type 2 diabetes mellitus in our hospital from July 2019 to June 2020 were randomly divided into a control group and a study group. The study group was treated with metformin and polyethylene glycol loxenatide injection, while the control group was treated with metformin. RESULTS: Before treatment, there was no significant difference in FPG (Fasting Blood Glucose) and PPG (Post Prandial Glycaemia) levels between the study group and the control group (p>0.05). After a treatment period, the FPG and PPG levels in the study group were significantly lower than those in the control group (p<0.05). With the longer treatment time, the patient's weight and BMI were lower (p<0.05). The weight and BMI of patients changed the least after one month of treatment, and the weight and BMI changed the most after more than seven months of treatment. After a period of treatment, the levels of FPG and PPG in the blood of male patients in the study group were significantly lower than those of female patients (p<0.05). After treatment, the TG level of the study group was significantly lower than that of the control group (p<0.05). In comparison, the HDL-C level was significantly higher than that of the control group (p<0.05). CONCLUSIONS: Lipid and glucose levels of type 2 diabetes mellitus patients with obesity have decreased after 12 weeks of polyethylene glycol loxanatide use. The weight of type 2 diabetes mellitus patients with obesity has changed after using polyethylene glycol loxenatide for a period of treatment. Among them, there is a certain relationship between body weight and treatment time, gender, and original body weight, which is worthy of further research and promotion in clinical practice. [ABSTRACT FROM AUTHOR]

The aim of this study was to observe the effects of remifentanil on organ damage and energy metabolism in lipopolysaccharide (LPS)-induced septic rats. A total of 45 clean-grade male Wistar rats (weight 270-320 g) were randomly divided into three groups: a control group, an LPS group, and an LPS with remifentanil treatment (LPS+REM) group. After 6 hours of modeling, the levels of tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6) in lung and kidney tissues of rats in each group were detected by ELISA. The activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in lung and kidney tissues were determined, and the content of lactic acid, pyruvate and epinephrine in heart and kidney tissues were detected. Reverse transcription polymerase chain reaction and the Western blot test were used to detect the expression of pyruvate dehydrogenase kinase 4 (PDK4) in the myocardial tissue. We found that remifentanil treatment inhibited the levels of IL-6, TNF-α, and MDA in the lung and kidneys 6 h after the administration of LPS and increased the level of SOD activity. Treatment with remifentanil reduced the expression of lactic acid, pyruvate, and epinephrine in the heart and kidney tissues and attenuated the expression of PDK4 messenger RNA and PDK4 protein in the myocardial tissue. We concluded that remifentanil mignt inhibit the release of tissue inflammatory factors, regulate the body's energy metabolism, and ultimately protect the sepsis tissue damage caused by LPS. [ABSTRACT FROM AUTHOR]

OBJECTIVE: Diabetes mellitus is a chronic metabolic disease which has an adverse impact on the quality of patient's life, so patients often need to receive treatment for a long time. Selection of medications with high therapeutics effects and low cost is very important for patients to take medicine for a longer period of time. Sitagliptin is a drug which is widely used in clinics and can effectively control blood glucose level. This article explores the pharmacoeconomic value of Sitagliptin in the treatment of diabetes mellitus. PATIENTS AND METHODS: A total of 100 patients with diabetes mellitus treated were recruited in this study. The patients were randomly divided into 4 groups with 25 cases in each group. Patients in group A were treated with pioglitazone, group B with Sitagliptin, group C with metformin and group D with glimepiride. The cost of the drugs, the treatment results and adverse effects were compared. RESULTS: Compared with group A, C and D, the cost-effectiveness ratio of group B was low (p<0.05), and the therapeutic effect was high (p<0.05). In addition, the incidence of adverse reactions in group B was lower than that in group A, C and D (p<0.05). There was no significant difference in the levels of FPG, 2hPG and HbAlc in patients among the four groups before treatment (p>0.05). After treatment, the levels of FPG, 2hPG and HbAlc in group B were significantly lower than those in groups A, C and D (p<0.05). Finally, there was no significant difference in waist circumference and BMI among the four groups before treatment (p>0.05). After treatment, the waist circumference and BMI in group B were lower than those in groups A, C and D (p<0.05). CONCLUSIONS: The application of Sitagliptin in the treatment of diabetic patients can effectively enhance the therapeutic effect. The cost effectiveness is satisfactory, and the blood glucose level can be maintained at a stable state. [ABSTRACT FROM AUTHOR]

To evaluate the impacts of guanidinoacetic acid (GAA) and coated folic acid (CFA) on growth performance, nutrient digestion and hepatic gene expression, fifty-two Angus bulls were assigned to four groups in a 2 × 2 factor experimental design. The CFA of 0 or 6 mg/kg dietary DM folic acid was supplemented in diets with GAA of 0 (GAA−) or 0·6 g/kg DM (GAA+), respectively. Average daily gain (ADG), feed efficiency and hepatic creatine concentration increased with GAA or CFA addition, and the increased magnitude of these parameters was greater for addition of CFA in GAA− diets than in GAA+ diets. Blood creatine concentration increased with GAA or CFA addition, and greater increase was observed when CFA was supplemented in GAA+ diets than in GAA− diets. DM intake was unchanged, but rumen total SCFA concentration and digestibilities of DM, crude protein, neutral-detergent fibre and acid-detergent fibre increased with the addition of GAA or CFA. Acetate:propionate ratio was unaffected by GAA, but increased for CFA addition. Increase in blood concentrations of albumin, total protein and insulin-like growth factor-1 (IGF-1) was observed for GAA or CFA addition. Blood folate concentration was decreased by GAA, but increased with CFA addition. Hepatic expressions of IGF-1, phosphoinositide 3-kinase, protein kinase B, mammalian target of rapamycin and ribosomal protein S6 kinase increased with GAA or CFA addition. Results indicated that the combined supplementation of GAA and CFA could not cause ADG increase more when compared with GAA or CFA addition alone. [ABSTRACT FROM AUTHOR]

Coated copper sulphate (CCS) could be used as a Cu supplement in cows. To investigate the influences of copper sulphate (CS) and CCS on milk performance, nutrient digestion and rumen fermentation, fifty Holstein dairy cows were arranged in a randomised block design to five groups: control, CS addition (7·5 mg Cu/kg DM from CS) or CCS addition (5, 7·5 and 10 mg Cu/kg DM from CCS, respectively). When comparing Cu source at equal inclusion rates (7·5 mg/kg DM), cows receiving CCS addition had higher yields of fat-corrected milk, milk fat and protein; digestibility of DM, organic matter (OM) and neutral-detergent fibre (NDF); ruminal total volatile fatty acid (VFA) concentration; activities of carboxymethyl cellulase, cellobiase, pectinase and α-amylase; populations of Ruminococcus albus, Ruminococcus flavefaciens and Fibrobacter succinogenes; and liver Cu content than cows receiving CS addition. Increasing CCS addition, DM intake was unchanged, yields of milk, milk fat and protein; feed efficiency; digestibility of DM, OM, NDF and acid-detergent fibre; ruminal total VFA concentration; acetate:propionate ratio; activity of cellulolytic enzyme; populations of total bacteria, protozoa and dominant cellulolytic bacteria; and concentrations of Cu in serum and liver increased linearly, but ruminal propionate percentage, ammonia-N concentration, α-amylase activity and populations of Prevotella ruminicola and Ruminobacter amylophilus decreased linearly. The results indicated that supplement of CS could be substituted with CCS and addition of CCS improved milk performance and nutrient digestion in dairy cows. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To investigate the effects of orthokeratology on biological parameters and visual quality of adolescents with low-grade corneal astigmatism myopia. PATIENTS AND METHODS: In this study, a total of 41 myopic adolescents were prescribed with orthokeratology glasses in our hospital from February 2018 to March 2019 and voluntarily cooperated with relevant examinations before and after wearing orthokeratology lenses. Patients' uncorrected distant visual acuity (UC-VA-D), uncorrected near visual acuity (UCVA-N) and naked eye near stereoacuity before wearing glasses, 1 month, and 3 months after wearing glasses were observed. The corneal astigmatism of patients was observed. The corneal endothelial cell density was observed. The dynamic adjustment function (NP, AF, NRA, PRA) values of patients were observed. The comparison of biological indexes in different time periods was observed. The changes of corneal curvature before wearing orthokeratology lens, 1 month and 3 months after wearing orthokeratology lens were observed. RESULTS: There were significant changes of patients 1 month after wearing orthokeratology lenses (p < 0.05), while there was no significant difference between 3 months after wearing the orthokeratology lenses and 1 month after wearing the orthokeratology lenses (p < 0.05). Patients' NCAV-D and UCVA-N were recorded by a conversion method of 5 points. There were differences in the NCVA-D, NCVA-N, naked eye near stereoacuity before, 1 month, and 3 months after wearing glasses (p < 0.05). By observing patients' biological indicators and dynamic adjustment, it was found that there were statistically significant differences in NP, AF, BRA and PRA before wearing the glasses, 1 month and 3 months after wearing the glasses (p < 0.05). CONCLUSIONS: The use of orthokeratology can greatly correct myopia patients' vision, improve their stereoscopic vision, control the progression of myopia, and improve their eye regulation, which is of high safety and great short-term effect. [ABSTRACT FROM AUTHOR]

The combined addition of branched-chain volatile fatty acids (BCVFAs) and folic acid (FA) could improve growth performance and nutrient utilization by stimulating ruminal microbial growth and enzyme activity. This study was conducted to evaluate the effects of BCVFA and FA addition on growth performance, ruminal fermentation, nutrient digestibility, microbial enzyme activity, microflora and excretion of urinary purine derivatives (PDs) in calves. Thirty-six Chinese Holstein weaned calves (60 ± 5.4 days of age and 107 ± 4.7 kg of BW) were assigned to one of four groups in a randomized block design. Treatments were control (without additives), FA (with 10 mg FA/kg dietary DM), BCVFA (with 5 g BCVFA/kg dietary DM) and the combined addition of FA and BCVFA (10 mg/kg DM of FA and 5 g/kg DM of BCVFA). Supplements were hand-mixed into the top one-third of total mixed ration. Dietary concentrate to maize silage ratio was 50 : 50 on a DM basis. Dietary BCVFA or FA addition did not affect dry matter intake but increased average daily gain (ADG) and feed conversion efficiency. Ruminal pH and ammonia N were lower, and total volatile fatty acids (VFAs) concentration was higher for BCVFA or FA addition than for control. Dietary BCVFA or FA addition did not affect acetate proportion but decreased propionate proportion and increased acetate to propionate ratio. Total tract digestibility of DM, organic matter, CP and NDF was higher for BCVFA or FA addition than for control. Dietary BCVFA or FA addition increased activity of carboxymethyl cellulase and cellobiase, population of total bacteria, fungi, Ruminococcus albus, R. flavefaciens, Fibrobacter succinogenes and Prevotella ruminicola as well as total PD excretion. Ruminal xylanase, pectinase and protease activity and Butyrivibrio fibrisolvens population were increased by BCVFA addition, whereas population of protozoa and methanogens was increased by FA addition. The BCVFA × FA interaction was significant for acetate to propionate ratio, cellobiase activity and total PD excretion, and these variables increased more with FA addition in diet without BCVFA than in diet with BCVFA. The data indicated that supplementation with BCVFA or FA increased ADG, nutrient digestibility, ruminal total VFA concentration and microbial protein synthesis by stimulating ruminal microbial growth and enzyme activity in calves. [ABSTRACT FROM AUTHOR]

This study evaluated the effects of rumen-protected folic acid (RPFA) and betaine (BT) on growth performance, nutrient digestion and blood metabolites in bulls. Forty-eight Angus bulls were blocked by body weight and randomly assigned to four treatments in a 2 × 2 factorial design. BT of 0 or 0·6 g/kg DM was supplemented to diet without or with the addition of 6 mg/kg DM of folic acid from RPFA, respectively. Average daily gain increased by 25·2 and 6·29 % for addition of BT without RPFA and with RPFA, respectively. Digestibility and ruminal total volatile fatty acids of neutral-detergent fibre and acid-detergent fibre increased, feed conversion ratio and blood folate decreased with the addition of BT without RPFA, but these parameters were unchanged with BT addition in diet with RPFA. Digestibility of DM, organic matter and crude protein as well as acetate:propionate ratio increased with RPFA or BT addition. Ruminal ammonia-N decreased with RPFA addition. Activity of carboxymethyl cellulase, cellobiase, xylanase, pectinase and protease as well as population of total bacteria, protozoa, Fibrobacter succinogenes and Ruminobacter amylophilus increased with RPFA or BT addition. Laccase activity and total fungi, Ruminococcus flavefaciens and Prevotella ruminicola population increased with RPFA addition, whereas Ruminococcus albus population increased with BT addition. Blood glucose, total protein, albumin, growth hormone and insulin-like growth factor-1 increased with RPFA addition. Addition of RPFA or BT decreased blood homocysteine. The results indicated that addition of BT stimulated growth and nutrient digestion in bulls only when RPFA was not supplemented. [ABSTRACT FROM AUTHOR]

OBJECTIVE: Recently, the role of long noncoding RNAs (lncRNAs) is vital in tumor progression. Our study aims to identify the role of SNHG16 in the metastasis of pancreatic carcinoma. PATIENTS AND METHODS: Real-Time quantitative Polymerase Chain Reaction (RT-qPCR) was used to measure SNHG16 expression in 56 pancreatic carcinoma patients' tissues. Function assays, including wound healing assay, and transwell assay, were conducted to detect the effect of SNHG16 on the metastasis of pancreatic carcinoma. Besides, the luciferase assay was performed to explore the underlying mechanism. RESULTS: The expression level of SNHG16 was upregulated in pancreatic carcinoma samples compared with adjacent tissues. Moreover, cell migration and cell invasion were repressed via the knockdown of SNHG16, while cell migration and cell invasion were promoted via the overexpression of SNHG16. Moreover, the expression of miR-200a-3p was upregulated via knockdown of SNHG16 while the expression of miR-200a-3p was downregulated via the upregulation of SNHG16 in vitro. Furthermore, it was discovered that SNHG16 acted as a competing endogenous RNA via sponging miR-200a-3p in pancreatic carcinoma. CONCLUSIONS: Our study suggests that SNHG16 acts as an oncogene in pancreatic carcinoma and promotes cell metastasis via sponging miR-200a-3p, which might be a novel therapeutic strategy in pancreatic carcinoma. [ABSTRACT FROM AUTHOR]

The aim of present paper is to study a constrained programming with generalized α-univex fuzzy mappings. In this paper we introduce the concepts of α-univex, α-preunivex, pseudo α-univex and α-unicave fuzzy mappings, and we discover that α-univex fuzzy mappings are more general than univex fuzzy mappings. Then, we discuss the relationships of generalized α-univex fuzzy mappings and get some properties. In the last, we derive necessary and sufficient Karush-Kuhn-Tucker conditions and its dual problems with generalized di erentiable α-univex fuzzy mappings for fuzzy constrained programming problem. [ABSTRACT FROM AUTHOR]

The effects of pantothenic acid (PA) and folic acid (FA) addition on digestibility coefficient, ruminal fermentation and urinary purine derivative (PD) excretion in dairy bulls were evaluated. Eight rumen-cannulated Holstein dairy bulls were allocated to a replicated 4 × 4 Latin square design according to a 2 × 2 factorial arrangement. Diets were supplemented with two levels of FA (0 or 8.0 mg/kg dietary dry matter [DM]) and two of PA (0 or 60 mg/kg DM). The PA × FA interaction was not significant for all variables. Both supplements increased DM intake and average daily gain, but decreased a feed conversion ratio. Digestibility of DM, organic matter, crude protein and neutral detergent fibre increased, but ether extract digestibility was unchanged for both supplements. Digestibility of acid detergent fibre only increased with FA supplementation. For both supplements, ruminal pH and ammonia nitrogen (N) decreased, but total volatile fatty acid (VFA) concentration increased. Acetate proportion only increased with FA supplementation. Propionate proportion decreased for both supplements. Consequently, the acetate to propionate ratio increased. For both supplements, activity of xylanase and pectinase, population of Ruminococcus albus , R. flavefaciens , Fibrobacter succinogenes and Ruminobacter amylophilus and total PD excretion increased. Additionally, activity of carboxymethylcellulase, cellobiase, α -amylase and protease, and population of total bacteria, fungi, protozoa, methanogens, Butyrivibrio fibrisolvens and Prevotella ruminicola increased with FA addition. The results suggested that PA and FA supplementation stimulated ruminal microbial growth and enzyme activity, resulting in an increased digestibility coefficient and ruminal total VFA concentration in dairy bulls. [ABSTRACT FROM AUTHOR]

In this paper, the open-circuit dc-winding-induced voltage in a wound field switched flux (WFSFs) machines is analyzed. The phenomenon of open-circuit dc-winding-induced voltage is illustrated and the mechanism is explained. Rotor skewing is proposed to reduce the open-circuit dc-winding-induced voltage, and the optimal skewing angle is analytically derived based on the analytically deduced harmonic orders of the open-circuit dc-winding-induced voltage. Finite-element (FE) analyses show that the open-circuit dc-winding-induced voltages in the analyzed 12-stator-pole partitioned stator WFSF machines having 10-, 11-, 13-, and 14-rotor-pole rotors can be effectively reduced by >94%, while the ac-winding phase-fundamental back-EMFs can be maintained by >95%. Twelve/ten-stator/rotor-pole prototypes with skewed and nonskewed rotors are built and tested to verify the analytical and FE results. [ABSTRACT FROM AUTHOR]

OBJECTIVE: Age-related macular degeneration (AMD) is mainly characterized by dysfunction of retinal pigment epithelium (RPE) cells. This study aimed to investigate the protective effects of resveratrol on oxidative damaged RPE cells. MATERIALS AND METHODS: Human D407 cells were divided into normal control (NC), H2O2 treated (H2O2, treating with H2O2 at a final concentration of 200 mol/l) and resveratrol treatment groups (treating with resveratrol at a concentration of 12.5, 25, 50 and 100 mg/l). Malondialdehyde (MDA) and superoxide dismutase (SOD) activities were examined using enzyme-linked immunosorbent assay (ELISA). 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl- 2-H-tetrazolium bromide (MTT) and cell count kit-8 (CCK-8) were used to examine cell viability. Cell cycle phase distribution and apoptosis of D407 cells were evaluated using flow cytometry assay. B-cell lymphoma-2 (Bcl-2) and cleaved caspase 3 expression were detected using quantitative real- time PCR (qRT-PCR) and Western blot assay, respectively. RESULTS: Resveratrol significantly decreased inhibitive ratios of D407 cell growth compared to that of H2O2 group (p<0.05). Resveratrol significantly increased SOD activity compared to that of H2O2 group (p<0.05). Resveratrol significantly reduced MDA activity compared to that of H2O2 group (p<0.05). Resveratrol affected cell cycle phase distribution of D407 cells compared to that of H2O2 group (p<0.05). Resveratrol significantly decreased the early stage and late stage apoptosis rates compared to that of H2O2 group (p<0.05). Resveratrol significantly enhanced Bcl-2 levels and decreased cleaved caspase 3 levels compared to that of H2O2 group (p<0.05). CONCLUSIONS: Resveratrol protected against the oxidative damage of RPE cells by modulating SOD/MDA activity and activating Bcl-2 expression. [ABSTRACT FROM AUTHOR]

DC winding induced voltage pulsation in the wound field synchronous machines (WFSMs) will cause dc winding current ripple, challenge the dc power supply, and deteriorate the control performance, especially at high speed. In this paper, the influence of dc winding configuration including the dc coil number and the parallel branch number on its induced voltage pulsation is investigated for WFSMs. Based on the modeling of both open-circuit and on-load dc winding induced voltages, the preferred dc winding configurations for WFSMs having various stator/rotor pole number combinations with double layer or single layer ac windings are obtained and validated by finite element (FE) analysis on four typical partitioned stator WFSMs (PS-WFSMs). A PS-WFSM prototype is built and tested to validate both analytical and FE analyses. [ABSTRACT FROM AUTHOR]

Highly soluble active materials, such as iodine, are of great potential to develop the research of rapid charge-discharge performance for Li-ion batteries. In this work, a new facile method is applied to construct the iodine/carbon electrode system, which is composed of a thin cathode film, prepared by directly mixing pure commercial iodine and superconducting carbon black, and a carbon nanotubes (CNTs) interlayer. The CNTs interlayer is inserted between pure iodine cathode and separator in the lithium-iodine battery. Electrochemical data reveals that the iodine cathode demonstrates a high capacity of 100 mAh g-1 and ~100% columbic efficiency after 5000 cycles at a rate of 100C, which shows a great cycle stability and superior high-rate capability, based on the contributions from both the confinement effect of iodine species in CNTs interlayer and the quick liquid-phase diffusion of Li ions in Li-I2 battery. [ABSTRACT FROM AUTHOR]

In order to increase the torque density of the five-phase machines, the third harmonic is commonly employed. In this paper, the optimal and critical ratios for the third harmonic current injection are analytically derived and validated by finite element (FE) analyses. It is found that the optimal and critical injection ratios along with the torque improvement are dependent on the ratio of the third harmonic back electromotive force to the fundamental one. In order to improve the electromagnetic torque contributed by the third harmonic component, this paper employs unequal tooth to increase both the fundamental and third harmonic winding factors. Meanwhile, the optimal unequal tooth width combination is deduced and demonstrated by the FE method. When the third harmonic current is injected under the same amplitude constraint, the output torque can be improved. When the third harmonic current is injected under the same RMS value constraint, for the ten-slot/eight-pole equal tooth machine, the output torque can barely be increased, whilst for the unequal tooth machine, the output torque can be increased by 2.1%. Finally, the ten-slot/eight-pole machine with unequal teeth is prototyped and tested to prove the analyses. [ABSTRACT FROM PUBLISHER]

The partitioned stator switched-flux permanent magnet (PM) (SFPM) (PS-SFPM) machine is a novel stator-PM machine in which PMs and armature windings are separately placed in two different stators. Compared with the conventional SFPM machine with a single stator in which both PMs and armature windings are located, the PS-SFPM machine can generate higher torque density due to the utilization of the machine's inner space. In this paper, the magnetic gearing effect in the PS-SFPM machine is investigated in terms of air-gap field harmonics based on a simple magnetomotive force (MMF)-permeance model. It is found that the PS-SFPM machine is one type of magnetically-geared machine, in which the modulations of rotor iron pieces to the open-circuit PM and armature reaction fields are similar to those found in the magnetic gear and the conventional magnetically geared machine. The pole-pair numbers and rotating speeds of the air-gap field harmonics obtained by the MMF-permeance model are verified by finite element (FE) analysis. More than 93% of the average electromagnetic torque is contributed by the main air-gap field harmonics having pole-pair numbers (2i - 1)\boldsymbol p PM and \rm| N\rm{r} \,\pm \, (2i-1)pPM| ( i = 1,2,3) in a PS-SFPM machine with pPM-pole-pair PMs and Nr-rotor-pole. A prototype machine is fabricated and tested to verify the FE analysis. [ABSTRACT FROM PUBLISHER]

The growth and alkaline phosphatase (ALP) activities of Sprague Dawley(SD) rat osteoblasts cultured in the nano-hydroxyapatite/polyetheretherketone (n-HA/PEEK) composite extracts and on the surfaces of these composites were investigated in this study. The osteoblasts of 24-h-old SD rats were cultured by modified enzymatic digestionin vitro. The ALP activities of the SD osteoblasts cultured in n-HA/PEEK composite extracts were measured and the mineralisation was studied by alizarin red staining. The morphological characteristics of the SD osteoblasts incubated on the n-HA/PEEK composite surfaces were observed by scanning electron microscopy and the ALP activity was also detected. The results showed that the n-HA/PEEK composites have good cytocompatibility with the SD osteoblasts and there were no obvious differences between PEEK and n-HA/PEEK composites in ALP activities and mineralisation of the SD osteoblasts cultured in their extracts. N-HA/PEEK composites could promote the growth of the SD osteoblasts on the surfaces of them and the improvement was related to the content and uniform distribution of n-HA in the n-HA/PEEK composites. The 5 vol.% n-HA/PEEK composites was promising to be used for bone substitute materials. [ABSTRACT FROM PUBLISHER]

Objective We aimed to investigate the prevalence of vitamin D deficiency in Chinese lupus patients and to assess the association between vitamin D levels and disease severity. Methods Serum levels of 25OHD3 in 121 patients with systemic lupus erythematosus (SLE) and 150 healthy controls were measured by electrochemiluminescence immunoassay. Data regarding demographics and clinical parameters were collected. Disease activity of SLE was evaluated according to the SLE Disease Activity Index (SLEDAI) score and irreversible organ damage by the Systemic Lupus International Collaborating Clinic/American College of Rheumatology, SLICC/ACR Damage Index (SDI). The multivariate logistic regression model was used to investigate the association between the degree of vitamin D deficiency and SLEDAI or SDI scores. Results The prevalence of vitamin D insufficiency (25OHD3 <30 ng/ml) and severe deficiency (25OHD3 <10 ng/ml) in SLE patients was 62.81% and 34.71%, respectively. Logistic regression analysis indicated that the cut-off point of 25OHD3 concentration was 10 ng/ml where its level was correlated with increased SLEDAI (OR 6.420, p = 0.006), but not with the SDI. In addition, hydroxychloroquine treatment lowered the SLEDAI increased by the severe 25OHD3 deficiency (OR 0.280, p = 0.008). Moreover, long disease duration (OR 1.014, p = 0.008) predicted moderate to severe organ damage. Conclusions Vitamin D deficiency is highly prevalent in patients with SLE. Severe deficiency increases the risk for moderate to severe disease activity, but not for organ damage. [ABSTRACT FROM AUTHOR]

In this paper, the end effects of a partitioned stator flux reversal permanent magnet (PM) machine with a surface-mounted PM (SPM) inner stator and a consequent pole PM (CPM) inner stator are comparatively analyzed. It is found that due to higher saturated inner stator steel in a CPM machine, it suffers from larger end effect than the SPM machine. Influence of armature reaction on end effect is investigated by a finite-element (FE) analysis, together with that of aspect ratio, i.e., axial stack length to stator outer diameter. Furthermore, it is found that in the CPM machine, the optimal PM arcs for the highest average electromagnetic torque predicted by 2-D and 3-D FE are different due to the variation of end effect with PM arc. Prototypes with both SPM and CPM inner stators are built and tested to verify the FE analysis. [ABSTRACT FROM PUBLISHER]

Previous observations have shown that the distribution of 36.2-GHz class I methanol megamaser (MM) emission in Arp 220 is highly correlated with the diffuse X-rays. On this basis it was suggested that methanol MM may be produced either by the effects of galactic-outflowdriven shocks and/or cosmic rays. Here we report the results of a single-dish survey undertaken with the Greenbank Telescope (GBT) to improve our understanding of the pumping conditions of extragalactic class I methanol masers and their relationship to starburst and feedback processes within the host galaxies, towards a sample which includes 16 galaxies which show both extended soft X-ray emission, and either OH or H2O MM emission. Large baseline ripples in the GBT spectra limited our results to tentative detections towards 11 of the target galaxies. Analysis of these tentative detections shows that there are significant correlations between the methanol intensity and the host-galaxy infrared, radio and OH MM emission, but no correlation with the X-ray and H2O MM emission. Some sources show methanol emission significantly offset from the systemic velocity of the galaxy (by up to 1000 km s-1) and we propose that these are associated with galactic-scale outflows from active galactic nuclei (AGNs) feedback. The combined observational properties suggest that class I methanol MMs are related to significant starburst and molecular outflow activity and hence may provide a potential probe of AGN feedback and starburst processes in the host galaxies. [ABSTRACT FROM AUTHOR]

This paper proposes a new partitioned stator flux reversal permanent magnet (PM) (PS-FRPM) machine having integer-slot overlapping windings, i.e., the slot number per pole per phase q is an integer, based on the magnetic gearing effect in stator-PM machines. Compared with the existing PS-FRPM machine with fractional-slot nonoverlapping windings, a more sinusoidal armature reaction magnetomotive force can be achieved in the proposed PS-FRPM machine with integer-slot overlapping windings, resulting in lower iron loss and PM loss. Electromagnetic performance of the PS-FRPM machine, which has q = 1 overlapping windings is analyzed and compared with PS-FRPM machine, which has q = 0.5 nonoverlapping windings by finite element (FE) analysis. FE results show that the proposed 24/10/12-outer-stator/rotor/inner-stator-pole PS-FRPM machine with q = 1 overlapping winding exhibits higher fundamental phase back-electromotive force, higher average electromagnetic torque and lower torque ripple, lower loss and higher efficiency, higher self-inductance but lower mutual inductance, higher d-axis inductance, and potential infinite flux-weakening capability compared with the 12/10/12-outer-stator/rotor/inner-stator-pole PS-FRPM machine with q = 0.5 nonoverlapping windings. Both prototype machines with q = 1 overlapping winding and q = 0.5 nonoverlapping windings are built and tested to verify the FE analyzes. [ABSTRACT FROM PUBLISHER]

This paper first investigates the flux-weakening control performance of two types of partitioned stator-switched flux permanent-magnet (PM) (PS-SFPM) machines and then proposes a novel mechanical flux-weakening technique. The PS-SFPM machine topologies, operation principles, and flux-weakening control method are described. In addition, it is shown that by mechanically varying the relative position of two stators of the PS-SFPM machine, the PM flux linkage can also be varied, and hence, it can provide improvement in the speed range and torque capability. The optimal relative mechanical angle is derived to enable the maximum torque capability and reduce or even avoid the flux-weakening current injected by the inverter. The proposed technique is verified by three-dimensional (3-D) finite-element (FE) analyses and experiments on the prototype machines. [ABSTRACT FROM AUTHOR]

This paper proposes a partitioned stator variable flux reluctance machine (PS-VFRM) in which field and armature windings are placed on two separate stators. The influence of different stator/rotor-pole combinations on the electromagnetic performance is examined using finite-element analysis. Candidate designs maximize the average electromagnetic torque. Compared against the conventional single stator VFRM, the proposed PS-VFRM is shown to provide 24.6% and 12.7% increases in torque density and efficiency, respectively. The static electromagnetic performance is validated using experimental prototypes. [ABSTRACT FROM PUBLISHER]

A novel type of partitioned stator switched flux permanent magnet (PS-SFPM) machine with either single-layer or double-layer windings is developed in this paper. The proposed PS-SFPM machines have two stators, which separately accommodate the armature windings and the PMs, and between which is the rotor made of iron pieces, while the number of stator poles with PMs may be equal or half of that with armature windings. All the machines are optimized under fixed copper loss for maximum average torque by genetic algorithm. Their electromagnetic performances are compared, such as open-circuit flux-linkages and back-electromotive forces (EMFs), cogging torque, static torque waveforms, average torque against current, PM utilization ratio, and flux-weakening performances. The results show that due to more PM usage and higher open-circuit back-EMF, the PS-SFPM machines having the number of stator poles with PMs the same as that with armature windings exhibit higher average torque, irrespective of the winding topologies, either single-layer or double-layer windings. However, the single-layer winding PS-SFPM machines having the number of stator poles with PMs half of that with armature windings have the best PM usage and the highest ratio of average torque to PM volume, as well as good flux-weakening capability. A prototype machine is manufactured and tested to validate the analyses. [ABSTRACT FROM PUBLISHER]

The partitioned stator (PS) machine adopts two stators to allocate windings and permanent magnets (PMs) separately, increasing the space for PMs as well as armature coils to boost the electromagnetic torque and improving the thermal condition of PMs. In this paper, the PS switched-flux PM (PS-SFPM) machine and the PS flux reversal PM (PS-FRPM) machine are proved to inherently share the same operating principle and similar machine topology but with interior PM (IPM) and surface-mounted PM stators, respectively. Furthermore, four globally optimized PS machines with different inner stator topologies are compared in terms of back EMF, cogging torque, electromagnetic torque, torque per PM volume, and flux-weakening capability. The results reveal that the spoke-shaped IPM (IPM-spoke) inner stator exhibits the highest back EMF and hence the highest average torque, while the I-shaped IPM (IPM-I) stator has the best flux-weakening capability, and the V-shaped IPM (IPM-V) produces the highest torque per PM volume. Furthermore, four machines are redesigned with the same PM usage volume, and the results show that the PS-SFPM machine still exhibits the highest back EMF as well as torque although with sacrificed advantages. The finite-element analyses and experiments are used to confirm the predictions. [ABSTRACT FROM AUTHOR]

In this paper, partitioned stator flux reversal permanent magnet (PS-FRPM) machines having different stator/rotor pole combinations with consequent-pole PM (CPM) stator are proposed and analyzed. Compared with the conventional 12-stator-pole PS-FRPM machines having 10-, 11-, 13-, and 14-rotor-pole rotors and surface-mounted PM (SPM) stator, the PM volume in the proposed PS-FRPM machines with CPM stator can be saved by 28.33%, 30%, 30%, and 33.33%, respectively, while the torque density are similar, i.e., 98.59%, 96.69%, 95.50%, and 97.15%, respectively. Besides, the proposed PS-FRPM machines with CPM stator can exhibit only <1% smaller efficiency compared with the existing PS-FRPM machines with SPM inner stator. [ABSTRACT FROM AUTHOR]

In this paper, a double stator (DS) wound field (WF) switched flux (SF) (DS-WFSF) machine is proposed. In the DS-WFSF machine, field and armature windings are separately placed in two different stators. Compared with the conventional WFSF machine with single stator, in which both field and armature windings are located, nonoverlapping concentrated windings and large slot areas can be obtained in the DS-WFSF machine. The proposed DS-WFSF machine exhibits >19% higher torque than the conventional WFSF machine, with both machines having the same space envelope and being globally optimized. The influence of leading design parameters, such as copper loss ratio between the field and armature windings, air-gap diameter, and rotor iron piece thickness and widths, on the average output torque is investigated for the DS-WFSF machines having 12/10, 12/11, 12/13, and 12/14 stator slots/rotor iron pieces. All the analyses are confirmed by both finite element and experimental results. [ABSTRACT FROM AUTHOR]

In this paper, novel partitioned stator doubly salient permanent magnet (PS-DSPM) machines with separated PM excitation and armature windings are proposed. Compared with conventional DSPM machines with single stator in which PMs are inserted in the yoke and windings are arranged on the teeth, the proposed PS-DSPM machine has two stators with PMs and windings located separately. Two sets of PS-DSPM machines, i.e., PS-DSPM-I and PS-DSPM-II machines, are proposed based on the conventional DSPM-I and DSPM-II machines in which the PMs are located on the stator yoke with intervals of every number of phases and every stator pole, respectively. The electromagnetic performance including back electromotive force (EMF) and torque characteristics of the proposed PS-DSPM machines are analyzed and compared with those of the conventional DSPM machines based on the optimal designs for the highest average electromagnetic torque by finite-element (FE) analysis in this paper. FE results show that the proposed PS-DSPM-I and PS-DSPM-II machines exhibit 8.49% and 207% higher torque density than the conventional DSPM-I and DSPM-II machines with same copper loss, respectively. The influence of main design parameters on the average electromagnetic torque in the proposed PS-DSPM machines is investigated, together with the conventional DSPM machines. A prototype machine of PS-DSPM-II is manufactured and tested to verify the FE analysis. [ABSTRACT FROM AUTHOR]

In this paper, switched flux permanent magnet (SFPM) machines are analyzed from the perspective of the air-gap field harmonics. It is found that the modulation of the salient rotor to PM and armature reaction fields in SFPM machines is similar to that of the iron pieces to those fields in the magnetic gear and magnetically geared machine. The magnetic gearing effect is analyzed in SFPM machines with different stator/rotor pole combinations, winding configurations, and stator lamination segment types by a simple magnetomotive force-permeance model, and validated by finite-element (FE) analysis. Different from fractional-slot surface-mounted PM machines in which the working air-gap field harmonic generates >95% of the average electromagnetic torque, >95% of the average electromagnetic torque in SFPM machines having \boldsymbol p\boldsymbol {s} stator pole pairs and nr rotor poles are contributed by several dominating field harmonics, i.e., rotating ones with \vert kn\boldsymbol {r}\,\pm \, (\textbf {2}\boldsymbol {i}-\textbf {1})\boldsymbol {p}\boldsymbol {s}\vert pole pair ( \boldsymbol k=\textbf 1, \boldsymbol i=\textbf 1, 2, 3 ) and static ones with (2\boldsymbol i-\textbf 1)\boldsymbol p\boldsymbol {s} pole pair ( \boldsymbol i=\textbf 1, 2, 3 ). The FE predicted average static torques in SFPM machines are validated by measurements on prototype machines. [ABSTRACT FROM AUTHOR]

Abstract: Both μ- and δ-opioid agonists selectively inhibit nociception but have little effect on other sensory modalities. Voltage-activated Ca2+ channels in the primary sensory neurons are important for the regulation of nociceptive transmission. In this study, we determined the effect of δ-opioid agonists on voltage-activated Ca2+ channel currents (ICa) in small-diameter rat dorsal root ganglion (DRG) neurons that do and do not bind isolectin B4 (IB4). The δ-opioid agonists [d-Pen2,d-Pen5]-enkephalin (DPDPE) and deltorphin II produced a greater inhibition of high voltage–activated ICa in IB4-negative than IB4-positive neurons. Furthermore, DPDPE produced a greater inhibition of N-, P/Q-, and L-type ICa in IB4-negative than IB4-positive neurons. However, DPDPE had no significant effect on the R-type ICa in either type of cells. We were surprised to find that DPDPE failed to inhibit either the T-type or high voltage–activated ICa in all the DRG neurons with T-type ICa. Double immunofluorescence labeling showed that the majority of the δ-opioid receptor-immunoreactive DRG neurons had IB4 labeling, while all DRG neurons immunoreactive to δ-opioid receptors exhibited Cav3.2 immunoreactivity. Additionally, DPDPE significantly inhibited high voltage–activated ICa in Tyrode''s or N-methyl-d-glucamine solution but not in tetraethylammonium solution. This study provides new information that δ-opioid agonists have a distinct effect on voltage-activated Ca2+ channels in different phenotypes of primary sensory neurons. High voltage–activated Ca2+ channels are more sensitive to inhibition by δ-opioid agonists in IB4-negative than IB4-positive neurons, and this opioid effect is restricted to DRG neurons devoid of functional T-type Ca2+ currents. [Copyright &y& Elsevier]

Unsteady premixed flames in narrow channels of different heights with adiabatic or isothermal walls are investigated numerically. It is assumed that the flame chemistry is modeled by an one-step overall reaction which simulates the reaction of a stoichiometric acetylene-air mixture. The numerical results show that, In the case of plane ignition, both channel height and thermal boundary condition affect flame structure formation. The increase of channel height is favor of the formation of tulip-shaped flame. Under a given channel height, a transition from the tulip-shaped flame to the mushroom-shaped flame occurs as the flame propagates from one end of channel to the other for isothermal cold walls. In addition, some parameters such as quenching temperature, quasi-extinction space and heat-supply rate are introduced to analyze the intrinsic relationships between flame propagation and quenching in narrow channels, which is called the criterion of flame propagation and quenching in narrow channels. [ABSTRACT FROM AUTHOR]

Abstract: Olvanil ((N-vanillyl)-9-oleamide), a non-pungent transient receptor potential vanilloid type 1 agonist, desensitizes nociceptors and alleviates pain. But its molecular targets and signaling mechanisms are little known. Calcium influx through voltage-activated Ca2+ channels plays an important role in neurotransmitter release and synaptic transmission. Here we determined the effect of olvanil on voltage-activated Ca2+ channel currents and the signaling pathways in primary sensory neurons. Whole-cell voltage-clamp recordings were performed in acutely isolated rat dorsal root ganglion neurons. Olvanil (1 μM) elicited a delayed but sustained inward current, and caused a profound inhibition (∼60%) of N-, P/Q-, L-, and R-type voltage-activated Ca2+ channel current. Pretreatment with a specific transient receptor potential vanilloid type 1 antagonist or intracellular application of 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid abolished the inhibitory effect of olvanil on voltage-activated Ca2+ channel current. Calmodulin antagonists (ophiobolin-A and calmodulin inhibitory peptide) largely blocked the effect of olvanil and capsaicin on voltage-activated Ca2+ channel current. Furthermore, calcineurin (protein phosphatase 2B) inhibitors (deltamethrin and FK-506) eliminated the effect of olvanil on voltage-activated Ca2+ channel current. Notably, 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid, calmodulin antagonists, and calcineurin inhibitors each alone significantly increased the amplitude of voltage-activated Ca2+ channel current. In addition, double immunofluorescence labeling revealed that olvanil induced a rapid internalization of CaV2.2 immunoreactivity from the membrane surface of dorsal root ganglion neurons. Collectively, this study suggests that stimulation of non-pungent transient receptor potential vanilloid type 1 inhibits voltage-activated Ca2+ channels through a biochemical pathway involving intracellular Ca2+-calmodulin and calcineurin in nociceptive neurons. This new information is important for our understanding of the signaling mechanisms of desensitization of nociceptors by transient receptor potential vanilloid type 1 analogues and the feedback regulation of intracellular Ca2+ and voltage-activated Ca2+ channels in nociceptive sensory neurons. [Copyright &y& Elsevier]