10 results on '"Wirachwong, Ponthip"'
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2. Safety and immunogenicity of an inactivated recombinant Newcastle disease virus vaccine expressing SARS-CoV-2 spike: Interim results of a randomised, placebo-controlled, phase 1 trial
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Pitisuttithum, Punnee, Luvira, Viravarn, Lawpoolsri, Saranath, Muangnoicharoen, Sant, Kamolratanakul, Supitcha, Sivakorn, Chaisith, Narakorn, Piengthong, Surichan, Somchaiya, Prangpratanporn, Sumalee, Puksuriwong, Suttida, Lamola, Steven, Mercer, Laina D., Raghunandan, Rama, Sun, Weina, Liu, Yonghong, Carreño, Juan Manuel, Scharf, Rami, Phumratanaprapin, Weerapong, Amanat, Fatima, Gagnon, Luc, Hsieh, Ching-Lin, Kaweepornpoj, Ruangchai, Khan, Sarwat, Lal, Manjari, McCroskery, Stephen, McLellan, Jason, Mena, Ignacio, Meseck, Marcia, Phonrat, Benjaluck, Sabmee, Yupa, Singchareon, Ratsamikorn, Slamanig, Stefan, Suthepakul, Nava, Tcheou, Johnstone, Thantamnu, Narumon, Theerasurakarn, Sompone, Tran, Steven, Vilasmongkolchai, Thanakrit, White, Jessica A, Bhardwaj, Nina, Garcia-Sastre, Adolfo, Palese, Peter, Krammer, Florian, Poopipatpol, Kittisak, Wirachwong, Ponthip, Hjorth, Richard, and Innis, Bruce L
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- 2022
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3. A Newcastle disease virus expressing a stabilized spike protein of SARS-CoV-2 induces protective immune responses
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Sun, Weina, Liu, Yonghong, Amanat, Fatima, González-Domínguez, Irene, McCroskery, Stephen, Slamanig, Stefan, Coughlan, Lynda, Rosado, Victoria, Lemus, Nicholas, Jangra, Sonia, Rathnasinghe, Raveen, Schotsaert, Michael, Martinez, Jose L., Sano, Kaori, Mena, Ignacio, Innis, Bruce L., Wirachwong, Ponthip, Thai, Duong Huu, Oliveira, Ricardo Das Neves, Scharf, Rami, Hjorth, Richard, Raghunandan, Rama, Krammer, Florian, García-Sastre, Adolfo, and Palese, Peter
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- 2021
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4. Safety and immunogenicity of a live attenuated influenza H5 candidate vaccine strain A/17/turkey/Turkey/05/133 H5N2 and its priming effects for potential pre-pandemic use: a randomised, double-blind, placebo-controlled trial
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Pitisuttithum, Punnee, Boonnak, Kobporn, Chamnanchanunt, Supat, Puthavathana, Pilaipan, Luvira, Viravarn, Lerdsamran, Hatairat, Kaewkungwal, Jaranit, Lawpoolsri, Saranath, Thanachartwet, Vipa, Silachamroon, Udomsak, Masamae, Wanibtisam, Schuetz, Alexandra, Wirachwong, Ponthip, Thirapakpoomanunt, Sit, Rudenko, Larisa, Sparrow, Erin, Friede, Martin, and Kieny, Marie-Paule
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- 2017
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5. An inactivated NDV-HXP-S COVID-19 vaccine elicits a higher proportion of neutralizing antibodies in humans than mRNA vaccination.
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Carreño, Juan Manuel, Raskin, Ariel, Singh, Gagandeep, Tcheou, Johnstone, Kawabata, Hisaaki, Gleason, Charles, Srivastava, Komal, Vigdorovich, Vladimir, Dambrauskas, Nicholas, Gupta, Sneh Lata, González Domínguez, Irene, Martinez, Jose Luis, Slamanig, Stefan, Sather, D. Noah, Raghunandan, Rama, Wirachwong, Ponthip, Muangnoicharoen, Sant, Pitisuttithum, Punnee, Wrammert, Jens, and Suthar, Mehul S.
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IMMUNOGLOBULINS ,COVID-19 vaccines ,INFLUENZA vaccines ,VACCINATION ,CLINICAL trials ,NEWCASTLE disease vaccines ,VIRAL antibodies - Abstract
NDV-HXP-S is a recombinant Newcastle disease virus–based vaccine against SARS-CoV-2, which expresses an optimized (HexaPro) spike protein on its surface. The vaccine can be produced in embryonated chicken eggs using the same process as that used for the production of the vast majority of influenza virus vaccines. Here, we performed a secondary analysis of the antibody responses after vaccination with inactivated NDV-HXP-S in a phase 1 clinical study in Thailand. The SARS-CoV-2 neutralizing and spike protein binding activity of NDV-HXP-S postvaccination serum samples was compared to that of samples from mRNA BNT162b2 (Pfizer) vaccinees. Neutralizing activity of sera from NDV-HXP-S vaccinees was comparable to that of BNT162b2 vaccinees, whereas spike protein binding activity of the NDV-HXP-S vaccinee samples was lower than that of sera obtained from mRNA vaccinees. This led us to calculate ratios between binding and neutralizing antibody titers. Samples from NDV-HXP-S vaccinees had binding to neutralizing activity ratios that were lower than those of BNT162b2 sera, suggesting that NDV-HXP-S vaccination elicits a high proportion of neutralizing antibodies and low non-neutralizing antibody titers. Further analysis showed that, in contrast to mRNA vaccination, which induces strong antibody titers to the receptor binding domain (RBD), the N-terminal domain, and the S2 domain, NDV-HXP-S vaccination induced an RBD-focused antibody response with little reactivity to S2. This finding may explain the high proportion of neutralizing antibodies. In conclusion, vaccination with inactivated NDV-HXP-S induces a high proportion of neutralizing antibodies and absolute neutralizing antibody titers that are comparable to those elicited by mRNA vaccination. A New(castle disease virus) vaccine for SARS-CoV-2: SARS-CoV-2 vaccines have been essential in combating the ongoing COVID-19 pandemic, but room for improvement remains. Here, Carreño et al. analyzed immune responses in individuals vaccinated with a Newcastle disease virus-based SARS-CoV-2 vaccine, NDV-HXP-S. This vaccine can be produced in embryonated chicken eggs, similar to the majority of influenza vaccines, making it readily translatable. The authors found that vaccination with NDV-HXP-S elicited a higher proportion of neutralizing antibodies against SARS-CoV-2 than vaccination with the BNT162b2 mRNA vaccine. NDV-HXP-S vaccinees also had a response more focused on the receptor binding domain. Together, these data highlight the promise of NDV-HXP-S, which has moved into a Phase III trial in Thailand. —CM [ABSTRACT FROM AUTHOR]
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- 2023
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6. A review of epidemic preparedness for influenza through local vaccine production: national security for Thailand.
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Pitisuttithum, Punnee and Wirachwong, Ponthip
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- 2019
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7. A live attenuated H5N2 prime- inactivated H5N1 boost vaccination induces influenza virus hemagglutinin stalk specific antibody responses.
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Kongchanagul, Alita, Samnuan, Karnyart, Wirachwong, Ponthip, Surichan, Somchaiya, Puthavathana, Pilaipan, Pitisuttithum, Punnee, and Boonnak, Kobporn
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HEMAGGLUTININ , *ANTIBODY formation , *INFLUENZA vaccines , *INFLUENZA A virus, H1N1 subtype , *INFLUENZA A virus , *H5N1 Influenza , *AVIAN influenza - Abstract
The emergence and spread of highly pathogenic avian influenza (H5N1) viruses have raised global concerns of a possible human pandemic, spurring efforts towards H5N1 influenza vaccine development and improvements in vaccine administration methods. We previously showed that a prime-boost vaccination strategy induces robust and broadly cross-reactive antibody responses against the hemagglutinin globular head domain. Here, we specifically measure antibodies against the conserved hemagglutinin stem region in serum samples obtained from the prior study to determine whether stalk-reactive antibodies can also be induced by the prime-boost regimen. Serum samples collected from 60 participants before vaccination and on days 7, 28 and 90 following boosting vaccination were used in this study. 40 participants received two doses of live attenuated H5N2 vaccine (LAIV H5N2) followed by one dose of inactivated H5N1 vaccine a year later, while 20 participants received only the inactivated H5N1 vaccine. We tested these serum samples for stalk-reactive antibodies via enzyme-linked immunosorbent (ELISA) and microneutralization assays. Stalk-specific antibody levels measured by both assays were found to be significantly higher in primed individuals than the unprimed group. ELISA results showed that 22.5, 70.5 and 57.5% of primed participants had a four-fold or more increase in stalk antibody titers on days 7, 28 and 90 following boosting vaccination, respectively; whereas the unprimed group had no increase. Peak geometric mean titers (GMT) for stalk antibodies in the LAIV H5N2 experienced group (24,675 [95% CI; 19,531–31,174]) were significantly higher than those who received only the inactivated H5N1 vaccine (8877 [7140–11,035]; p < 0·0001). Moreover, stalk antibodies displaying neutralizing activity also increased in primed participants, but not in the unprimed group. Our finding emphasizes the importance of prime-boost vaccination for effectively inducing stalk antibodies, which is an attractive target for developing vaccines that induce stalk reactive antibodies. [ABSTRACT FROM AUTHOR]
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- 2020
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8. Development of influenza vaccine production capacity by the Government Pharmaceutical Organization of Thailand: Addressing the threat of an influenza pandemic
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Surichan, Somchaiya, Wirachwong, Ponthip, Supachaturas, Wutichai, Utid, Kanchala, Theerasurakarn, Sompone, Langsanam, Pimsuk, Lakornrach, Pattharachai, Nitisaporn, Ladda, Chansikkakorn, Chanpen, Vangkanonta, Wilak, Kaweepornpoj, Ruangchai, Poopipatpol, Kittisak, Thirapakpoomanunt, Sit, Srichainak, Somchai, Artavatkun, Witit, Chokevivat, Vichai, and Wibulpolprasert, Suwit
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SEASONAL influenza , *INFLUENZA vaccines , *DRUG development , *DOSAGE forms of drugs , *PHARMACEUTICAL industry , *PANDEMICS , *CLINICAL trials - Abstract
Abstract: In 2005, a year after highly pathogenic avian influenza outbreaks in Thailand, the Thai Government issued a National Strategy Plan for Pandemic Influenza Preparedness, a major objective of which was the domestic production of seasonal influenza vaccine. It was considered that sustained influenza vaccine production was the best guarantee of a pandemic vaccine in the event of a future pandemic. The Government decided to provide funds to establish an industrial-scale influenza vaccine production plant, and gave responsibility for this challenging project to the Government Pharmaceutical Organization (GPO). In 2007, with support from the World Health Organization (WHO), the GPO started to develop egg-based, trivalent inactivated influenza vaccine (IIV) in a renovated pilot plant. In early 2009, during the second year of the project, the GPO turned its attention to develop a pandemic live attenuated influenza vaccine (PLAIV) against the influenza A (H1N1) virus. By December 2010, the H1N1 PLAIV had successfully completed Phase II clinical trials and was awaiting registration approval from the Thai Food and Drug Administration (TFDA). The GPO has also started to develop an H5N2 PLAIV, which is expected to enter clinical trials in January 2011. The next step in 2011 will be the development and clinical evaluation of seasonal LAIV. To meet the needs of the national seasonal influenza vaccination programme, the GPO aims to produce 2 million doses of trivalent IIV in 2012 and progressively increase production to the maximum annual capacity of 10 million doses. This article relates how influenza vaccine production capacity was developed and how major challenges are being met in an expeditious manner, with strong local and global commitment. [Copyright &y& Elsevier]
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- 2011
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9. Safety and Immunogenicity of an Inactivated Recombinant Newcastle Disease Virus Vaccine Expressing SARS-CoV-2 Spike: Interim Results of a Randomised, Placebo-Controlled, Phase 1/2 Trial.
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Pitisuttithum P, Luvira V, Lawpoolsri S, Muangnoicharoen S, Kamolratanakul S, Sivakorn C, Narakorn P, Surichan S, Prangpratanporn S, Puksuriwong S, Lamola S, Mercer LD, Raghunandan R, Sun W, Liu Y, Carreño JM, Scharf R, Phumratanaprapin W, Amanat F, Gagnon L, Hsieh CL, Kaweepornpoj R, Khan S, Lal M, McCroskery S, McLellan J, Mena I, Meseck M, Phonrat B, Sabmee Y, Singchareon R, Slamanig S, Suthepakul N, Tcheou J, Thantamnu N, Theerasurakarn S, Tran S, Vilasmongkolchai T, White JA, Garcia-Sastre A, Palese P, Krammer F, Poopipatpol K, Wirachwong P, Hjorth R, and Innis BL
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Background: Production of affordable coronavirus disease 2019 (COVID-19) vaccines in low- and middle-income countries is needed. NDV-HXP-S is an inactivated egg-based Newcastle disease virus vaccine expressing the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It's being developed in Thailand, Vietnam, and Brazil; herein are initial results from Thailand., Methods: This phase 1 stage of a randomised, dose-escalation, observer-blind, placebo-controlled, phase 1/2 trial was conducted at the Vaccine Trial Centre, Mahidol University (Bangkok). Healthy adults aged 18-59 years, non-pregnant and negative for SARS-CoV-2 antibodies were eligible. Participants were block randomised to receive one of six treatments by intramuscular injection twice, 28 days apart: 1 µg±CpG1018 (a toll-like receptor 9 agonist), 3 µg±CpG1018, 10 µg, or placebo. Participants and personnel assessing outcomes were masked to treatment. The primary outcomes were solicited and spontaneously reported adverse events (AEs) during 7 and 28 days after each vaccination, respectively. Secondary outcomes were immunogenicity measures (anti-S IgG and pseudotyped virus neutralisation). An interim analysis assessed safety at day 57 in treatment-exposed individuals and immunogenicity through day 43 per protocol. ClinicalTrials.gov ( NCT04764422 )., Findings: Between March 20 and April 23, 2021, 377 individuals were screened and 210 were enrolled (35 per group); all received dose one; five missed dose two. The most common solicited AEs among vaccinees, all predominantly mild, were injection site pain (<63%), fatigue (<35%), headache (<32%), and myalgia (<32%). The proportion reporting a vaccine-related AE ranged from 5·7% to 17·1% among vaccine groups and was 2·9% in controls; there was no vaccine-related serious adverse event. The 10 µg formulation's immunogenicity ranked best, followed by 3 µg+CpG1018, 3 µg, 1 µg+CpG1018, and 1 µg formulations. On day 43, the geometric mean concentrations of 50% neutralising antibody ranged from 122·23 IU/mL (1 µg, 95% CI 86·40-172·91) to 474·35 IU/mL (10 µg, 95% CI 320·90-701·19), with 93·9% to 100% of vaccine groups attaining a ≥4-fold increase over baseline., Interpretation: NDV-HXP-S had an acceptable safety profile and potent immunogenicity. The 3 µg and 3 µg+CpG1018 formulations advanced to phase 2., Funding: National Vaccine Institute (Thailand), National Research Council (Thailand), Bill & Melinda Gates Foundation, National Institutes of Health (USA).
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- 2021
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10. Anti-cancer and anti-angiogenic effects of curcumin and tetrahydrocurcumin on implanted hepatocellular carcinoma in nude mice.
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Yoysungnoen P, Wirachwong P, Changtam C, Suksamrarn A, and Patumraj S
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- Animals, Cell Line, Tumor, Dose-Response Relationship, Drug, Humans, Inhibitory Concentration 50, Male, Mice, Mice, Inbred BALB C, Mice, Nude, Neoplasm Transplantation, Angiogenesis Inhibitors pharmacology, Antineoplastic Agents pharmacology, Carcinoma, Hepatocellular drug therapy, Curcumin analogs & derivatives, Curcumin pharmacology, Liver Neoplasms drug therapy
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Aim: To determine the effect of tetrahydrocurcumin (THC) on tumor angiogenesis compared with curcumin (CUR) by using both in vitro and in vivo models of human hepatocellular carcinoma cell line (HepG2)., Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay was used for testing the anti-proliferating activities of CUR and THC. In male BALB/c nude mice, 2 multiply 10(6) human HepG2 cells were inoculated onto a dorsal skin-fold chamber. One day after HepG2 inoculation, the experimental groups were fed oral daily with CUR or THC (300 mg/kg or 3000 mg/kg). On d 7, 14 and 21, the tumor microvasculature was observed using fluorescence videomicroscopy and capillary vascularity (CV) was measured., Results: Pathological angiogenic features including microvascular dilatation, tortuosity, and hyper-permeability were observed. CUR and THC could attenuate these pathologic features. In HepG2-groups, the CV were significantly increased on d 7 (52.43%), 14 (69.17%), and 21 (74.08%), as compared to controls (33.04%, P < 0.001). Treatment with CUR and THC resulted in significant decrease in the CV (P < 0.005 and P < 0.001, respectively). In particular, the anti-angiogenic effects of CUR and THC were dose-dependent manner. However, the beneficial effect of THC treatment than CUR was observed, in particular, from the 21 d CV (44.96% and 52.86%, P < 0.05)., Conclusion: THC expressed its anti-angiogenesis without any cytotoxic activities to HepG2 cells even at the highest doses. It is suggested that anti-angiogenic properties of CUR and THC represent a common potential mechanism for their anti-cancer actions.
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- 2008
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