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Summary: Background: It is unknown if early COVID-19 monoclonal antibody (mAb) therapy can reduce risk of Long COVID. The mAbs amubarvimab/romlusevimab were previously demonstrated to reduce risk of hospitalization/death by 79%. This study assessed the impact of amubarvimab/romlusevimab on late outcomes, including Long COVID. Methods: Non-hospitalized high-risk adults within 10 days of COVID-19 symptom onset enrolled in a randomized, double-blind, placebo-controlled phase 2/3 trial of amubarvimab/romlusevimab for COVID-19 treatment. Late symptoms, assessed using a participant-completed symptom diary, were a pre-specified exploratory endpoint. The primary outcome for this analysis was the composite of Long COVID by participant self-report (presence of COVID-19 symptoms as recorded in the diary at week 36) or hospitalization or death by week 36. Inverse probability weighting (IPW) was used to address incomplete outcome ascertainment, giving weighted risk ratios (wRR) comparing amubarvimab/romlusevimab to placebo. Findings: Participants received amubarvimab/romlusevimab (n = 390) or placebo (n = 390) between January and July 2021. Median age was 49 years, 52% were female, 18% Black/African American, 49% Hispanic/Latino, and 9% COVID-19-vaccinated at entry. At week 36, 103 (13%) had incomplete outcome ascertainment, and 66 (17%) on amubarvimab/romlusevimab and 92 (24%) on placebo met the primary outcome (wRR = 0.70, 95% confidence interval (CI) 0.53–0.93). The difference was driven by fewer hospitalizations/deaths with amubarvimab/romlusevimab (4%) than placebo (13%). Among 652 participants with available diary responses, 53 (16%) on amubarvimab/romlusevimab and 44 (14%) on placebo reported presence of Long COVID. Interpretation: Amubarvimab/romlusevimab treatment, while highly effective in preventing hospitalizations/deaths, did not reduce risk of Long COVID. Additional interventions are needed to prevent Long COVID. Funding: National Institute of Allergy and Infectious Diseases of the National Institutes of Health. Amubarvimab and romlusevimab supplied by Brii Biosciences.

Youth of Color in the United States are often leaders in movements for social justice. Evidence suggests that organizing has a positive macro-therapeutic effect on the mental health of young organizers; however, they can also experience strain and become targets of the very systems they are trying to change. In a community-based participatory action research study, three organizations that train youth of Color in organizing in Brooklyn, New York City held focus groups with youth and adult staff. The focus groups examined the strains experienced by youth organizers and the strategies adult partners use to prepare organizers to maintain hope and well-being. Findings suggest four key emergent strategies: (a) provide an emotional homespace to process the rub between worlds, (b) actively shape the long view on systems change, (c) increase self-care skills and emotional preparation for organizing, and (d) promote healing by building leader(full) communities.

This narrative case study describes the use of improvement science methods to strengthen the effectiveness of field experiences for teacher candidates. As part of a networked improvement community, we sought to reduce variation in the quality of field experience by providing a cohesive network of high-quality coaching supports. Using design-based methods grounded in the principles of improvement, we analyzed seven learning, design, and testing rounds to reduce variation in teacher candidates' experiences as student teachers or interns. Results from early learning rounds suggested considerable structural variability in how supervisors conducted their POP cycles. The structural variability from these rounds led to two key change ideas to address variation in teacher candidates field experiences: a common POP cycle protocol and the use of a POP-cycle-aligned reflection form. The principles and tools of improvement highlighted small but significant changes to how supervisors support teachers within their field experiences.

The objective of this research was to assess and quantify the potential vitamin A losses that occur during the manufacturing of pet feed and premix, as well as during their extended storage periods. This trial was conducted at a commercial feeder mill that utilized a standard commercial dog feed along with a corresponding vitamin-mineral premix. The calculated amount of vitamin A supplemented in the feed, in addition to the endogenous vitamins present in the ingredients, was adjusted to 18,000 IU/kg of feed. Five 500 g feed samples were collected at each of the predefined critical points throughout the manufacturing process (after mixing, milling, preconditioner, and extrusion/drying processes) to verify the stability of vitamin A during feed production. Additionally, various samples were collected at regular intervals of 30, 60, 90, 120, and 180 days during the storage of the premix to assess the stability of vitamin A. Vitamin A analyses in the samples were performed using high-performance liquid chromatography. The variables were assessed for normality using the Shapiro–Wilk test, followed by analysis of variance (ANOVA) and Tukey’s test to compare the differences between the manufacturing process and premix shelf life. The statistical significance was set at 95%. The vitamin losses during the pre-conditioning process were 26%, and during the extrusion-drying processes, the losses were 34% when compared to the initial analyzed value. However, no differences were observed in other processes. There were no significant differences observed in recovered vitamin levels in the premix during its shelf-life (p = 0.484). The study indicated that the primary vitamin A losses in pet feed manufacturing processes occur during the pre-conditioning and drying/extrusion steps. However, it is worth noting that no significant losses of vitamin A were found during the premix storage phase.

This study aimed to investigate the effects of replacing inorganic trace minerals (ITM) with organic trace minerals (OTM) on growth performance, body composition, gut histomorphometry, digestive enzyme activity, apparent digestibility coefficients (ADC), and mineral balance in juvenile Nile tilapia, Oreochromis niloticus. Fish (n = 432; 2.5 ± 0.5 g) were randomly distributed into four groups with four replicates each (27 fish per replicate). A control diet (gross 15.2 MJ gross energy kg−1; 367.3 g crude protein kg−1) was elaborated to meet the dietary requirements of zinc (Zn), manganese (Mn), and copper (Cu) using ITM in the sulfate form (ITM100). From the control diet, three diets were elaborated using OTM to supply 100% (OTM100), 75% (OTM75), and 50% (OTM50) of Zn, Mn, and Cu ITM of the control diet, respectively, supplied in the glycinate form. Fish were hand-fed six times daily for 8 weeks. Growth performance was not changed by dietary treatments. Fish fed on the diet OTM50 showed higher whole-body and vertebrae ash contents and higher whole-body calcium (Ca), Zn, and iron (Fe) retention than those fed on the control diet. The activity of digestive enzymes and the ADC of energy and nutrients, including Zn, Mn, and Cu, were similar in fish fed on diets OTM50 and ITM100. A reduction in Zn (−39.8%), Mn (−11.1%), and Cu (−14.0%) loss was observed in fish fed on the diet OTM50 than in fish fed diet ITM100. The dietary treatments did not affect the gut histomorphometry. In conclusion, the inclusion of OTM in Nile tilapia’s diets represents a promising strategy to reduce 50% of ITM sources by utilizing Zn, Mn, and Cu OTM as glycinates without detrimental effects on growth performance, nutrient utilization, and gut function. These results additionally reinforce the environmental benefits of using OTM for precise mineral nutrition in aquaculture.

Across the globe, the emergence of COVID-19 led to widespread, sudden suspension of in-person instruction, displacing more than 1.5 billion learners (Capilla et al., 2020). Addressing the gap in research on emergency remote teaching (ERT), this empirical study draws on insights from semi-structured interviews with 10 in-service and five pre-service teachers, who navigated the transition both as K-12 teachers and graduate students, participating in weekly mentoring for English language learners, online curricular modules, face-to-face discussions (until the transition to ERT), mixed-reality simulation teaching with coaching, and written reflections. Using a sensemaking theoretical framework, our study examines the following questions: (1) What were the main challenges and opportunities of ERT as experienced by this cohort of language teachers? (2) How did the dual role of being a K-12 teacher and graduate student provide a unique lens for navigating these challenges and opportunities? (3) What tools or supports helped these language teachers through the transition to ERT? Thematic analysis revealed three themes (emotion, shared meaning, and technology) and illuminated connection as an overarching theme. Findings suggest that the experience of navigating the transition from both positions led to greater empathy, increased facility using technology, and a growing support network of fellow teacher-learners.

Abstract Mutations in the SNCA gene cause autosomal dominant Parkinson’s disease (PD), with loss of dopaminergic neurons in the substantia nigra, and aggregation of α-synuclein. The sequence of molecular events that proceed from an SNCA mutation during development, to end-stage pathology is unknown. Utilising human-induced pluripotent stem cells (hiPSCs), we resolved the temporal sequence of SNCA-induced pathophysiological events in order to discover early, and likely causative, events. Our small molecule-based protocol generates highly enriched midbrain dopaminergic (mDA) neurons: molecular identity was confirmed using single-cell RNA sequencing and proteomics, and functional identity was established through dopamine synthesis, and measures of electrophysiological activity. At the earliest stage of differentiation, prior to maturation to mDA neurons, we demonstrate the formation of small β-sheet-rich oligomeric aggregates, in SNCA-mutant cultures. Aggregation persists and progresses, ultimately resulting in the accumulation of phosphorylated α-synuclein aggregates. Impaired intracellular calcium signalling, increased basal calcium, and impairments in mitochondrial calcium handling occurred early at day 34–41 post differentiation. Once midbrain identity fully developed, at day 48–62 post differentiation, SNCA-mutant neurons exhibited mitochondrial dysfunction, oxidative stress, lysosomal swelling and increased autophagy. Ultimately these multiple cellular stresses lead to abnormal excitability, altered neuronal activity, and cell death. Our differentiation paradigm generates an efficient model for studying disease mechanisms in PD and highlights that protein misfolding to generate intraneuronal oligomers is one of the earliest critical events driving disease in human neurons, rather than a late-stage hallmark of the disease.

While the GDPR and other EU laws seek to mitigate a range of potential harms associated with smart cities, the compliance with and enforceability of these regulations remain an issue. In addition, these proposed regulations do not sufficiently address the collective harms associated with the deployment of biometric technologies and artificial intelligence. Another relevant question is whether the initiatives put forward to secure fundamental human rights in the digital realm account for the issues brought on by the deployment of technologies in city spaces. In this special issue, we employ the smart city notion as a point of connection for interdisciplinary research on the human rights implications of the algorithmic, biometric and smart city technologies and the policy responses to them. The articles included in the special issue analyse the latest European regulations as well as soft law, and the policy frameworks that are currently at work in the regions where the GDPR does not apply.

Smart city technologies can have detrimental effects on human rights, making it crucial to mitigate them in the R&D phase. This qualitative socio-legal study of the Helsinki metropolitan area (HMA) explores how public funding for smart city research and development (R&D), and the data protection by design principle (DPbD) of the General Data Protection Regulation (GDPR), facilitate the development of human rights compliant technology. Our study shows that the tension between the neoliberal logic of smart cities and that human rights compliance extends from the local to the global level. High compliance and localisation costs, one-sided inputs and a push for scalability in smart city technology development in Finland and other EU states may attract companies to overlook human rights risks and pursue markets outside the EU with lower standards of respect for human rights and the rule of law. We propose policy measures to facilitate human rights compliant smart city R&D, localisation and procurement, and discuss human rights due diligence and export control measures as means to mitigate the potential adverse effects of smart city technology exported from the EU. The study contributes to research on human rights-based approaches to smart city technology development and European innovation and export policy, with attention given to the role of public R&D funding agencies.

In the setting of ischemic steal syndrome with tissue loss, patients with radiocephalic fistulas have limited options to preserve their conduit and treat their ischemic symptoms. To address this, we have proposed the technique of proximalization through one incision of a wrist arteriovenous fistula (POWR) with distal revascularization with interval ligation (DRIL) procedure. In the present retrospective, single-center, case series, we evaluated the outcomes of three patients with radiocephalic fistulas who had undergone POWR DRIL from 2017 to 2021. Their ischemic symptoms were monitored for regression. All three patients showed signs of regressing ischemia. The POWR DRIL represents an efficient procedure to address tissue loss and preserve the autogenous conduit.

Wealth inequality is rising, and millennials will be the future recipients of the largest intergenerational wealth transfer. Meanwhile, there is a need to move more money to support transformative social justice movements. This study examines the impact of spirituality as a motivator for the social justice movement giving among progressive young adult activists with wealth and class privilege, organizing toward the equitable redistribution of wealth, land, and power. Using survey data (n = 560), regressions and mediation models suggest that spiritual motivation was a significant positive predictor of how much participants monetarily gave to social justice movements. While religion did not significantly predict movement giving, indirect effects models showed that spirituality positively mediated the effect of being raised with any religion on movement giving compared to those indicating no religion. Implications are explored for how transformative organizing models draw upon secular spiritual practices in their pursuit of individual and collective change. [ABSTRACT FROM AUTHOR]

Grades in introductory STEM courses can impact intrinsic motivation (IM) and self-efficacy (SE). We used an exploratory approach to examine trends in grades and how IM and SE impact students' grades. It is known that most first-generation (FG) college students are from underrepresented backgrounds (URM), and that these students are least likely to persist in STEM fields. Therefore, gaining a better understanding of motivation in our most underserved students could be critical to improving their success in introductory biology courses and ultimately future persistence in STEM. We found that, on average, URM FG students received the lowest final grades and experienced the greatest declines in IM and SE. This research informs the way we may think about changes in motivation and self-efficacy in our introductory biology courses as these lay the foundation for future learning and success. [ABSTRACT FROM AUTHOR]

This study evaluated the impact of increasing dietary β‐mannanase (BM) supplementation (Natupulse TS, BASF, Ludwigshafen am Rhein, Germany) on fecal viscosity and pH, apparent digestibility coefficients (ADCs) of energy and nutrients, including amino acids, fecal nutrient loss in contents of soybean meal (SBM)‐based diets fed to juvenile Nile tilapia, Oreochromis niloticus. A total of 504 fingerlings (initial body weight of 31.5 ± 4.2 g) were randomly distributed into 24 aquaria (70 L each) in a completely randomized design using six dietary treatments and four replicates of 21 fish per aquarium. Fish were fed diets containing BM at 0, 1600, 3200, 4800, 6400, and 8000 TMU (thermostable endo‐1,4‐BM units) kg−1 and hand‐fed six times a day until apparent satiety for 30 days. Chromium oxide was employed as an indigestible marker. The ADC of gross energy, crude protein, crude lipids, and essential amino acids exhibited a quadratic effect with the maximum point in fish fed 4818, 4722, 4615, and 4816 TMU kg−1 BM, respectively. The digestible energy (DE) content of diets presented a quadratic effect, and the maximum point was obtained in fish fed 4803 TMU BM kg−1 diet, which improved the DE content by +1.2 MJ kg−1 relative to the BM‐unsupplemented diet. The fecal pH, fecal viscosity, organic matter loss, and nitrogen loss presented a quadratic effect with the minimum point in fish fed 4810, 4839, 4750, and 4801 TMU BM kg−1 diet, respectively. Principal component analysis indicated that dietary BM at 4800 and 6400 TMU BM kg−1 diet was associated with improvements in the ADC of gross energy, crude protein, and crude lipids. In conclusion, including 4803 TMU BM kg−1 diet effectively improves DE content and optimizes nutrient digestibility and feces pH and viscosity in Nile tilapia fed an SBM‐based diet. [ABSTRACT FROM AUTHOR]

Youth of Color, especially those in households with low income, experience multiple stressors and trauma that affect their well-being. Few studies examine the impact of youth engagement in leadership and organizing to address systemic inequity on their mental health and well-being. In a community-based participatory action research design, three organizations which train youth of Color in organizing in Brooklyn, New York, held four focus groups (n = 43, ages 14-24 years) to examine the impact of organizing on youth mental health and well-being. Key emergent themes of youth organizing include (a) storytelling as therapeutic; (b) group leadership as strengthening personal and collective power, hopefulness, and a sense of protection; and (c) the strain of navigating their hopes and current reality. This study has important implications for actions programs can take who seek to engage youth of Color in organizing in communities and institutions impacted by historical trauma and current day systemic inequity.

Background: Current femoral bone loss classification systems in revision total hip arthroplasty were created at a time when the predominant reconstructive methods used cylindrical porous-coated cobalt-chrome stems. As these stems have largely been replaced by fluted-tapered titanium stems, the ability of these classification systems to help guide implant selection is limited. The purpose of this study was to describe a novel classification system based on contemporary reconstructive techniques. Methods: We reviewed the charts of all patients who underwent femoral component revision at our institution from 2007 through 2019. Preoperative images were reviewed, and FBL was rated according to the Paprosky classification and compared to ratings using our institution’s NCS. Rates of reoperation at the time of most recent follow-up were determined and compared. Results: Four-hundred and forty-two femoral revisions in 330 patients with a mean follow-up duration of 2.7 years were identified. Femoral type according to Paprosky and NCS were Paprosky I (36, 8.1%), II (61, 13.8%), IIIA (180, 40.7%), IIIB (116, 26.2%), and IV (49 11.1%) and NCS 1 (35, 7.9%), 2 (364, 82.4%), 3 (8, 1.8%), 4 (27, 6.1%), and 5 (8, 1.8%). Of the 353 nonstaged rTHAs, there were 42 cases requiring unplanned reoperation (11.9%), including infection (18, 5.1%), instability (10, 2.8%), femoral loosening (5, 1.4%), and various other causes (9, 2.5%). The NCS was more predictive of reoperation than the Paprosky classification (Fisher’s exact test, P = .008 vs P = ns, respectively). Conclusion: We present a novel femoral classification system that can help guide contemporary implant selection.

School-based sports and physical education play an important role in the development of youth (Jones, Edwards, et al., 2017), but participation in athletics is unequal for lesbian, gay, bisexual, transgender, and queer (LGBTQ) youth compared to their straight/cisgender peers [Greenspan, S. B., Griffith, C., & Murtagh, E. F. (2017). LGBTQ youths' school athletic experiences: A 40-year content analysis in nine flagship journals. "Journal of LGBT Issues in Counseling," 11(3), 190-200]. Sport cultures, generally, recreate dynamics of exclusion for marginalized youth. However, there are opportunities to transform these spaces into more inclusive and positive environments to support positive growth for all young people [Newman, T., Alvarez, A., & Kim, M. (2017). An experiential approach to sport-based positive youth development. "Journal of Experiential Education," 40(3), 308-322]. Our study uses a data set of adolescents, collected as part of a community-based participatory action research project led by high school students in southeast Michigan, USA. Respondents reported their sexual and gender identities, as well as experiences in youth sport, as well as safety using sex-segregated facilities (i.e., bathrooms and locker-rooms). Using mediation models based on linear regression, we found evidence that LGBQ high school students play sports at a significantly lower rate compared to straight students, and among those who play sports, LGBTQ respondents felt significantly less safe compared to straight and cisgender students. Opposite to the LGBQ and trans youth in this study, straight/cisgender youth also reported feeling safer using all facilities. The mediation models suggest that these inequalities help to explain disparities in rates of sports participation and feelings of safety while participating. These findings have important implications for policy, practice, and future research.

This study aimed to investigate the effects of replacing inorganic trace minerals (ITM) with organic trace minerals (OTM) on growth performance, body composition, gut histomorphometry, digestive enzyme activity, apparent digestibility coefficients (ADC), and mineral balance in juvenile Nile tilapia, Oreochromis niloticus. Fish (n = 432; 2.5 ± 0.5 g) were randomly distributed into four groups with four replicates each (27 fish per replicate). A control diet (gross 15.2 MJ gross energy kg−1; 367.3 g crude protein kg−1) was elaborated to meet the dietary requirements of zinc (Zn), manganese (Mn), and copper (Cu) using ITM in the sulfate form (ITM100). From the control diet, three diets were elaborated using OTM to supply 100% (OTM100), 75% (OTM75), and 50% (OTM50) of Zn, Mn, and Cu ITM of the control diet, respectively, supplied in the glycinate form. Fish were hand‐fed six times daily for 8 weeks. Growth performance was not changed by dietary treatments. Fish fed on the diet OTM50 showed higher whole‐body and vertebrae ash contents and higher whole‐body calcium (Ca), Zn, and iron (Fe) retention than those fed on the control diet. The activity of digestive enzymes and the ADC of energy and nutrients, including Zn, Mn, and Cu, were similar in fish fed on diets OTM50 and ITM100. A reduction in Zn (−39.8%), Mn (−11.1%), and Cu (−14.0%) loss was observed in fish fed on the diet OTM50 than in fish fed diet ITM100. The dietary treatments did not affect the gut histomorphometry. In conclusion, the inclusion of OTM in Nile tilapia's diets represents a promising strategy to reduce 50% of ITM sources by utilizing Zn, Mn, and Cu OTM as glycinates without detrimental effects on growth performance, nutrient utilization, and gut function. These results additionally reinforce the environmental benefits of using OTM for precise mineral nutrition in aquaculture. [ABSTRACT FROM AUTHOR]

Abstract Electronic Health (eHealth) is the use of information and communication technologies for health and plays a significant role in improving public health. The rapid expansion and development of eHealth initiatives allow researchers and healthcare providers to connect more effectively with patients. The aim of the CIHLMU Symposium 2020 was to discuss the current challenges facing the field, opportunities in eHealth implementation, to share the experiences from different healthcare systems, and to discuss future trends addressing the use of digital platforms in health. The symposium on eHealth explored how the health and technology sector must increase efforts to reduce the obstacles facing public and private investment, the efficacy in preventing diseases and improving patient quality of life, and the ethical and legal frameworks that influence the proper development of the different platforms and initiatives related to the field. This symposium furthered the sharing of knowledge, networking, and patient/user and practitioner experiences in low- and middle-income countries (LMIC) in both public and private sectors.

IntroductionDown Syndrome (DS) adults experience accumulation of Alzheimer's disease (AD)-like amyloid plaques and tangles and a high incidence of dementia and could provide an enriched population to study AD-targeted treatments. However, to evaluate effects of therapeutic intervention, it is necessary to dissociate the contributions of DS and AD from overall phenotype. Imaging biomarkers offer the potential to characterize and stratify patients who will worsen clinically but have yielded mixed findings in DS subjects.MethodsWe evaluated 18F fluorodeoxyglucose positron emission tomography (PET), florbetapir PET, and structural magnetic resonance (sMR) image data from 12 nondemented DS adults using advanced multivariate machine learning methods.ResultsOur results showed distinctive patterns of glucose metabolism and brain volume enabling dissociation of DS and AD effects. AD-like pattern expression corresponded to amyloid burden and clinical measures.DiscussionThese findings lay groundwork to enable AD clinical trials with characterization and disease-specific tracking of DS adults.

Summary: This study aimed to evaluate 2 phytases, at 2 supplementation doses, in broiler diets with nutritional matrices with reduction in Ca:total P (tP) ratio. One-day-old male chicks (n = 3,960) were distributed in an arrangement of 12 treatments, with 10 replicates each; the experimental unit consisted of a floor pen with 33 birds each. Trial lasted up to 42 D, and birds were given the experimental diets: T1—positive control 1 (high Ca:tP ratio—recommended amount); T2—negative control 1 (reduction of 0.16% Ca and 0.16% available P-AP); T3–T2 + 500 FTU/kg enzyme A; T4–T2 + 500 FTU/kg enzyme B; T5–T2 + 1000 FTU/kg enzyme A; T6–T2 + 1000 FTU/kg enzyme B; T7–positive control 2 (low Ca:tP ratio); T8—negative control 2 (reduction of 0.16% Ca and 0.16% AP); T9–T8 + 500 FTU/kg enzyme A; T10–T8 + 500 FTU/kg enzyme B; T11–T8 + 1000 FTU/kg enzyme A; and T12–T8 + 1000 FTU/kg enzyme B. At 21 and 42 D of age, performance variables were recorded (body weight, body weight gain, feed intake, and feed conversion ratio) and tibial bone composition (Ca and P) and resistance was determined (strength at rupture). Results indicate that enzyme supplementation was efficient, regardless of Ca:tP ratio. The highest phytase dose presented superior results, especially for the 21-D evaluation. Phytase “B,” a bacterial product, showed superior response on performance and bone quality.

Abstract Introduction Amyloid measurement provides important confirmation of pathology for Alzheimer's disease (AD) clinical trials. However, many amyloid positive (Am+) early‐stage subjects do not worsen clinically during a clinical trial, and a neurodegenerative measure predictive of decline could provide critical information. Studies have shown correspondence between perfusion measured by early amyloid frames post‐tracer injection and fluorodeoxyglucose (FDG) positron emission tomography (PET), but with limitations in sensitivity. Multivariate machine learning approaches may offer a more sensitive means for detection of disease related changes as we have demonstrated with FDG. Methods Using summed dynamic florbetapir image frames acquired during the first 6 minutes post‐injection for 107 Alzheimer's Disease Neuroimaging Initiative subjects, we applied optimized machine learning to develop and test image classifiers aimed at measuring AD progression. Early frame amyloid (EFA) classification was compared to that of an independently developed FDG PET AD progression classifier by scoring the FDG scans of the same subjects at the same time point. Score distributions and correlation with clinical endpoints were compared to those obtained from FDG. Region of interest measures were compared between EFA and FDG to further understand discrimination performance. Results The EFA classifier produced a primary pattern similar to that of the FDG classifier whose expression correlated highly with the FDG pattern (R‐squared 0.71), discriminated cognitively normal (NL) amyloid negative (Am–) subjects from all Am+ groups, and that correlated in Am+ subjects with Mini‐Mental State Examination, Clinical Dementia Rating Sum of Boxes, and Alzheimer's Disease Assessment Scale–13‐item Cognitive subscale (R = 0.59, 0.63, 0.73) and with subsequent 24‐month changes in these measures (R = 0.67, 0.73, 0.50). Discussion Our results support the ability to use EFA with a multivariate machine learning–derived classifier to obtain a sensitive measure of AD‐related loss in neuronal function that correlates with FDG PET in preclinical and early prodromal stages as well as in late mild cognitive impairment and dementia. Highlights The summed initial post‐injection minutes of florbetapir positron emission tomography correlate with fluorodeoxyglucose. A machine learning classifier enabled sensitive detection of early prodromal Alzheimer's disease. Early frame amyloid (EFA) classifier scores correlate with subsequent change in Mini‐Mental State Examination, Clinical Dementia Rating Sum of Boxes, and Alzheimer's Disease Assessment Scale–13‐item Cognitive subscale. EFA classifier effect sizes and clinical prediction outperformed region of interest standardized uptake value ratio. EFA classification may aid in stratifying patients to assess treatment effect.

Abstract The objective of this work was to evaluate the effect of the use of hybrid phytase, alone or combined with carbohydrases, in poultry diets with nutritional reductions of calcium, available phosphorus, and metabolizable energy on the nutrient digestibility and bone quality of broiler chickens. A total of 1,875 broilers were distributed in five treatments in a completely randomized design, with 15 replicates of 25 chickens each. The treatments consisted of a positive control feed (T1) and of four negative controls (T2 to T5): T1, basal diet (BD) with corn and soybean; T2 and T3, BDs with reductions of 70 and 100 kcal kg-1 metabolizable energy, respectively, and both with reductions of 0.16% Ca and 0.15% available P; and T4 and T5, BDs with the same nutritional reductions, but supplemented with enzymes, i.e., T4 = T2 + 500 phytase units (FTU) per kilogram and T5 = T3 + 500 FTU kg-1 + 560 xylanase units (TXU) per kilogram + 250 glucanase units (TGU) per kilogram. The use of 500 FTU kg-1 hybrid phytase in pelleted corn-soybean meal diets allows a good digestive performance by broilers and replaces the nitrogen-corrected apparent metabolizable energy at 70 kcal kg-1, as well as 0.16% Ca and 0.15% available P.