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Copyright of Tropical Animal Health & Production is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Tropical Animal Health & Production is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Abstract Background Envenoming by south Asian hump-nosed pit vipers (Genus: Hypnale) is a significant health issue in Sri Lanka and in peninsular India. Bites by these snakes frequently lead to local envenoming, coagulopathy and acute renal failure even resulting in death. Recently the genus was revised and the existence of three species viz H. hypnale, H. nepa and H. zara were recognized. There is, however, a paucity of information on the toxicity of the venoms of these species. Hence, we compared the toxic effects of the three Hypnale venoms using BALB/c mice. Findings Intraperitoneal median lethal doses (LD50) for H. hypnale, H. zara and H. nepa venoms were 1.6, 6.0 and 9.5 μg protein/g respectively. Minimum haemorrhagic doses for venoms of H. hypnale, H. zara and H. nepa were 3.4, 11.0 and 16.6 μg protein/mouse respectively. The minimum necrotic doses for the same venoms were 15.0, 55.1 and 68.2 μg protein/mouse respectively. Severe congestion and petecheal haemorrhages were observed in lungs, kidneys, liver and the alimentary tract. Histopathogical examination of kidneys revealed proximal tubular cell injury and acute tubular necrosis with intact basement membrane indicating possible direct nephrotoxicity. Hypnale venoms caused pulmonary oedema, hepatocellular degeneration and necrosis, focal neuronal degeneration in brain and extramedullary haemopoiesis in spleen. H. hypnale venom caused all above histopathological alterations at lower doses compared to the other two. Conclusion Hypnale venoms cause similar pathological changes with marked differences in the severity of the toxic effects in vivo. Therefore, differences in the severity of the clinical manifestations could possibly be seen among bite victims of the three Hypnale species.

Intra-aural tick infestation was detected in 29 patients presenting with acute ear pain in the central province of Sri Lanka during June 1999 to January 2002. The ages ranged from 9 months to 67 years. All showed oedematous aural canals and enlarged adjacent lymph nodes. Of 30 ticks removed, 29 were nymphs of Dermacentor auratus and 1 and male Hyalomma marginatum isaaci.

Objective: To determine the degree of soil pollution in an area of high prevalence and the risk of exposure to geohelminth infection., Design: A cross-sectional study., Setting: A division of a large tea plantation in Kandy., Subjects: All persons resident in one division of the tea plantation, above the age of 1 year., Measurements: Prevalence of geohelminth infection measured by microscopic examination of faeces of the study group. Soil samples from 38 specific locations, obtained on 3 different occasions, about 6 months apart, analysed for helminth ova. The average temperature, rainfall and the number of rainy days during the sampling period., Results: Of the 99 subjects of the study group, 77.7% were positive for at least one geohelminth infection. The prevalence rates for ascariasis, trichuriasis and hookworm infection were 54%, 28%, and 22% respectively. At the first sampling, 10.5% of the soil samples were positive for helminth ova with 7.8% and 2.6% samples showing Ascaris and Necator ova respectively. At the second sampling of the same locations nearly 40% of the samples were positive for helminth ova with 21.6%, 7.8% and 10.5% of the samples positive for Ascaris, Trichuris and Toxocara ova respectively. At the third sampling, 26.2% of the samples were positive with 21%, 2.6% and 2.6% of the samples positive for Ascaris, Trichuris and Toxocara ova respectively. Necator ova were found only once in 2.6% of the samples of the first sampling. The larger number of samples positive for helminth ova were in the second and the third samplings which coincided with high rainfall in the pre-sampling period., Conclusions: The prevalence of geohelminthiases among the study group was high. Results of soil analysis confirmed continued contamination of soil in and around the home gardens and the work areas with human and dog faeces. With the knowledge of exact locations of soil pollution it was possible to advice the management on remedial measures.