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Turmeric extract is a β-diketone polyphenol extract isolated from theCurcuma longa L, a plant of the genus Curcuma of the Zingberaceae family. It mainly includes curcumin (CUR), demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC). It is a commonly used food, natural pigment and cosmetic additive, which has various pharmacological activities, such as hypolipidemia, antioxidant, anti-inflammation, antiviral and anti-Alzheimer's disease. However, poor stability and low bioavailability limit the clinical application of curcuminoids. Curcumin hydrogenated metabolites also have pharmacological activity, and they are more stable and bioavailable than curcumin. The metabolites of curcuminoids (CUR, DMC and BDMC) in turmeric extract in rats were identified. Turmeric extract was suspended in 0.5% carboxymethlcellulose sodium (CMC-Na). Healthy male Sprague Dawley (SD) rats in drug group were gavaged with a single dose of 1 703 mg/kg turmeric extract (approximately 480 mg/kg curcuminoids), and the rats in control group were given equal volume of 05% CMC-Na solution. And then, plasma at different time points was collected after oral gavage. Urine, faeces and bile were collected after oral gavage in rats within 12 h. Plasma were prepared by protein precipitation method, the urine, faeces and bile were extracted by methanol. The biological samples of faeces, urine, bile and plasma were analyzed by ultra high performance liquid chromatography-quadrupole extractive orbitrap mass spectrometry (UPLC-Q-Extractive MS) in negative ion mode. The accurate molecular weight, primary and secondary mass spectrometry information of metabolites in faeces, urine, bile and plasma were identified. As a result, a total of 59 metabolites (B1-W12) were identified. B3, B7, B8, B11, B12, B13, C3, D3, D5, D8, D10, W1, W2, W4, W5, W7 and W10 were new metabolites. W3/W6, W11/W12 and W12 were firstly found in plasma, faces and urine, respectively. The results showed that the main metabolic pathways of curcumin were glucuronidation, sulfation, reductive hydrogenation, oxidation, reduction, hydroxylation, dehydroxylation and their composite reactions. This method can provide a theoretical basis for the later screening of active metabolites of curcumin compounds.

The wild species Thinopyrum intermedium (genome JJJSJSStSt) serves as a valuable germplasm resource providing novel diseases resistance and agronomically important genes for wheat improvement. Two wheat-Th. intermedium partial amphiploids, TAI7045 (2n = 56) and 78784 (2n = 56), exhibit high resistance to stripe rust and powdery mildew, and their chromosome constitutions have been characterized. With the aim to transfer novel resistance genes from Th. intermedium, the crosses of common wheat line MY11 with TAI7045 and 78784 were produced, and their individual F2-F5 progenies were characterized using sequential non-denaturing fluorescence in situ hybridization (ND-FISH) and molecular markers. We identified a set of wheat-Th. intermedium addition lines, involving the chromosomes 1St-JS, 2St, 2St-JS, 3St, 4J, 4St, 5St, 5J.St, 6JS.J, and 7JS. Above all, the stable wheat-Th. intermedium small segmental translocation lines with chromosomes 4DS.4DL-4StL-4DL-4JL and 4DS.4DL-4StL-4DL were selected. Combining data from specific marker amplification and resistance evaluation, we mapped the gene(s) for resistance to powdery mildew and stripe rust in the 233.56–329.88 Mb region of the long arm of the 4St chromosome from the reference Th. intermedium genome. The new wheat-Th. intermedium introgressions will be used as novel germplasm for breeding purposes. [ABSTRACT FROM AUTHOR]

Chuanwang Xiaoyan (CWXY) capsule is primarily used to treat a variety of acute and chronic inflammations, including acute and chronic pharyngitis and tonsillitis. However, a systematic study of its chemical constituents is still not available. This study evaluated the chemical constituents in vitro and metabolite profiles in vivo of CWXY using ultra‐high‐performance liquid chromatography (UHPLC) coupled with Q‐Exactive Orbitrap mass spectrometry, and the pharmacokinetic behaviors of the nine main components in rats were detected using ultra‐high‐performance liquid chromatography‐triple quadrupole‐mass spectrometry (UPLC‐QQQ‐MS/MS). A total of 92 chemical constituents in CWXY were preliminarily identified in vitro. After oral administration to rats, 56 prototype components and 128 metabolites of CWXY were detected in the biological samples of rat plasma, urine, bile, and feces. Of these prototype components and metabolites, seven new compounds, namely M15, M16, M25, M30, M31, M71, and M128, were detected for the first time. The quantitation method of nine components in rat plasma was developed using a pharmacokinetic study. To the best of our knowledge, this study was the first to investigate the pharmacokinetic behavior of triumbelletin. [ABSTRACT FROM AUTHOR]

The perennial species Dasypyrum breviaristatum (genome [V.sup.b]) contains many potentially valuable genes for the improvement of common wheat. Construction of a detailed karyotype of D. breviaristatum chromosomes will be useful for the detection of Dasypyrum chromatin in wheat background. We established the standard karyotype of 1[V.sup.b]-7[V.sup.b] chromosomes through nondenaturing fluorescence in situ hybridization (ND-FISH) technique using 28 oligonucleotide probes from the wheat - D. breviaristatum partial amphiploid TDH-2 (AABB[V.sup.b][V.sup.b]) and newly identified wheat - D. breviaristatum disomic translocation and addition lines D2138 (6[V.sup.b]S.2[V.sup.b]L), D2547 (4[V.sup.b]), and D2532 (3[V.sup.b]S.6[V.sup.b]L) by comparative molecular marker analysis. The ND-FISH with multiple oligo probes was conducted on the durum wheat - D. villosum amphiploid TDV-1 and large karyotype differences between D. breviaristatum and D. villosum was revealed. These ND-FISH probes will be valuable for screening the wheat - Dasypyrum derivative lines for chromosome identification, and the newly developed wheat - D. breviaristatum addition lines may broaden the gene pool of wheat breeding. The differences between D. villosum and D. breviaristatum chromosomes revealed by ND-FISH will help us understand evolutionary divergence of repetitive sequences within the genus Dasypyrum. Key words: Dasypyrum breviaristatum, Dasypyrum villosum, oligo probes, chromosome identification, ND-FISH. L'espece perenne Dasypyrum breviaristatum (genome [V.sup.b]) contient plusieurs genes potentiellement utiles pour l'amelioration genetique du ble tendre. La production d'un caryotype detaille des chromosomes du D. breviaristatum sera utile pour detecter la chromatine du Dasypyrum au sein d'un fond genetique de ble. Les auteurs ont etabli un caryotype standard pour les chromosomes 1[V.sup.b]-7[V.sup.b] via hybridation in situ en fluorescence en conditions non-denaturantes (ND-FISH) a l'aide de 28 sondes oligonucleotidiques. Ces analyses ont ete realisees sur la lignee TDH-2, un amphiploide partiel ble - D. breviaristatum (AABB[V.sup.b][V.sup.b]), ainsi que sur des lignees disomiques de translocation et d'addition [D2138 (6[V.sup.b]S.2[V.sup.b]L), D2547 (4[V.sup.b]) et D2532 (3[V.sup.b]S.6[V.sup.b]L)] nouvellement identifiees par analyse avec des marqueurs moleculaires. L'analyse ND-FISH avec de nombreuses sondes a ete realisee sur TDV-1, un amphiploide ble dur - D. villosum, et de grandes differences caryotypiques entre D. breviaristatum et D. villosum ont ete revelees. Ces sondes ND-FISH seront utiles pour l'identification des chromosomes chez des lignees derivees de croisements ble - Dasypyrum. Les lignees d'addition ble - D. breviaristatum nouvellement produites permettront d'elargir l'assise genetique pour l'amelioration genetique du ble. Les differences revelees par analyse ND-FISH entre les chromosomes du D. villosum et du D. breviaristatum aideront a mieux connaitre la divergence evolutive des sequences repetees au sein du genre Dasypyrum. [Traduit par la Redaction] Mots-cles: Dasypyrum breviaristatum, Dasypyrum villosum, sondes oligonucleotidiques, identification des chromosomes, ND-FISH., Introduction The genus Dasypyrum contains two species, namely the annual D. villosum (L.) P. Candargy (2x = 2n = 14) and the perennial D. breviaristatum (L.) Frederiksen (2x = 2n [...]

Infrared thermography testing (IRT) has been widely used in the defect detection of composite materials. However, the identification of defects characteristics is unsatisfying due to the interference of factors such as uneven background and noise in the original thermal image sequence. A novel thermography-based defect detection method with the semantic segmentation network is proposed to enhance the defect contrast and extract perfect features. To Figure out the abnormal distribution of temperature field in thermal images, AG-UNet was used with a spatial self-attention gate module to extract spatial features of thermal images. The 3D-UNet network was obtained by the 3D convolution module and the temporal convolution module to extract thermal temporal and spatial features simultaneously which could help the defect segmentation in thermal videos. Compared with traditional algorithms such as principal component analysis (PCA), thermographic signal reconstruction (TSR), and fast Fourier transform (FFT), defects detection results were significantly enhanced with the proposed method, and defects of smaller diameter-to-depth ratio can be detected by deep learning models. [ABSTRACT FROM AUTHOR]

Gastric cancer has a high rate of recurrence, and as such, immunotherapy strategies are being investigated as a potential therapeutic strategy. Although the involvement of immune checkpoints in immunotherapy is well studied, biomechanical cues, such as target cell stiffness, have not yet been subject to the same level of investigation. Changes in the cholesterol content of the cell membrane directly influence tumor cell stiffness. Here, we investigated the effect of cholesterol on NK cell‐mediated killing of gastric cancer stem‐like cells. We report that surviving tumor cells with stem‐like properties elevated cholesterol metabolism to evade NK cell cytotoxicity. Inhibition of cholesterol metabolism enhances NK cell‐mediated killing of gastric cancer stem‐like cells, highlighting a potential avenue for improving immunotherapy efficacy. This study suggests a possible effect of cancer cell stiffness on immune evasion and offers insights into enhancing immunotherapeutic strategies against tumors. [ABSTRACT FROM AUTHOR]

More than half of non‐muscle‐invasive bladder cancer (NMIBC) patients eventually relapse even if treated with surgery and BCG without optional bladder‐preserving therapy. This study aims to investigate the antitumor activity and safety of a HER2‐targeted antibody‐drug conjugate, RC48‐ADC, intravesical instillation for NMIBC treatment. In this preclinical study, it is revealed that human epidermal growth factor receptor 2 (HER2) expression scores of 1+, 2+, and 3+ are recorded for 16.7%, 56.2%, and 14.6% of NMIBC cases. The antitumor effect of RC48‐ADC is positively correlated with HER2 expression in bladder cancer (BCa) cell lines and organoid models. Furthermore, RC48‐ADC is revealed to exert its antitumor effect by inducing G2/M arrest and caspase‐dependent apoptosis. In an orthotopic BCa model, tumor growth is significantly inhibited by intravesical instillation of RC48‐ADC versus disitamab, monomethyl auristatin E, epirubicin, or phosphate‐buffered saline control. The potential toxicity of intravesical RC48‐ADC is also assessed by dose escalation in normal nude mice and revealed that administration of RC48‐ADC by intravesical instillation is safe within the range of effective therapeutic doses. Taken together, RC48‐ADC demonstrates promising antitumor effects and safety with intravesical administration in multiple preclinical models. These findings provide a rational for clinical trials of intravesical RC48‐ADC in NMIBC patients. [ABSTRACT FROM AUTHOR]

Lingyang Qingfei pills (LQP), the renowned traditional Chinese medicine recipe, have been extensively utilized for the therapy of xerostomia, sore throat, bronchiolitis, and pneumonia in clinics. However, its phytochemicals remain equivocal, which severely limits the development of quality control and activity mechanisms. In the current research, a trusted method founded on ultra‐high performance liquid chromatography with Quadrupole‐Exactive Orbitrap mass spectrometry technique was proposed for the comprehensive screening of in vitro and in vivo chemical compositions of LQP. As a consequence, 239 constituents were preliminarily characterized, 37 of which were accurately confirmed by reference standards. In addition, a total of 208 xenobiotics, containing 71 absorbed prototypes and 137 metabolites, were revealed in rat plasma, bile, urine, and feces, respectively. The metabolic reaction of hydrolysis, hydroxylation, methylation, glycosylation, sulfation, and mixed‐mode was detected in the biotransformations of flavonoids, terpenoids, alkaloids, anthraquinones, organic acids, phenylpropanoids, and so forth. And 12 of the metabolites were new compounds. This experiment acted as the first reference for chemical substances and metabolites of LQP, which could provide valuable chemical information for further clarifying pharmacodynamic substances and pharmacokinetic studies. [ABSTRACT FROM AUTHOR]

To improve the fatigue life of the key component and the surface properties of the 30CrNi2MoVA steel material, advanced ultrasonic-milling composite superficial treatment was performed. The microstructure, surface roughness, friction and wear performance, surface hardness, fatigue life and environmental experiments of the steel with and without ultrasonic-milling have been carried out in detail. In comparison with those of the traditional dry cutting, the results show that the surface roughness of the samples after the advanced ultrasonic-milling surface modification fluctuates about 0.32 μm, and the surface hardness is increased by about 40% compared with the matrix hardness, and the fatigue life of the pump head connection shaft has been increased by more than 11 times. Advanced ultrasonic-milling surface modification technology can increase the local residual compressive stress and wear resistance on the material surface, which can make the material have better surface properties. [ABSTRACT FROM AUTHOR]

Objectives: This retrospective study was conducted to assess the efficacy and safety of high intensity focused ultrasound (HIFU) in combination with chemotherapy compared with chemotherapy alone in treating patients with unresectable locally advanced pancreatic cancer (LAPC). Methods: The data of unresectable LAPC patients who received chemotherapy with or without HIFU ablation were retrieved retrospectively. The overall survival (OS), objective response rate (ORR), cancer antigen 19‐9 response rate, and safety were compared between these two groups before and after propensity score matching (PSM). Results: Overall, 254 patients with LAPC were included, of whom 92 underwent HIFU ablation. After PSM to control for potential biases, HIFU was associated with improved OS (12.8 versus 12.2 months, log‐rank P =.046), as compared to patients without HIFU ablation. Patients with numeric rating scale (NRS) less than 4, and receiving HIFU ablation were significantly associated with improved OS (adjusted hazard ratio [aHR] = 0.365 [95% confidence interval (CI) = 0.148–0.655], P =.002; aHR = 0.490 [95% CI = 0.250–0.961], P =.038; respectively) by multivariate analyses with the adjustment of age, NRS, and tumor size. ORR was also observed to be higher in HIFU group of 30.0% than in the chemotherapy group of 13.3% (P =.039). No severe adverse events of special interest or HIFU‐caused deaths were observed. Conclusions: Patients with unresectable LAPC who received gemcitabine‐based chemotherapy might benefit from additional HIFU ablation. [ABSTRACT FROM AUTHOR]

Data-driven methods based on samples from a supervisory control and data acquisition system have been widely applied in water-supply-network burst detection to save unexpected economic and labor costs. However, the class imbalance problem in actual on-site monitoring needs to be revised to improve the performance of data-driven methods. In this study, we proposed a domain adaptation method to generate minor-category samples (pipeline-burst samples in general) of arbitrary pipe networks utilizing theoretical hydraulic models. The proposed method transferred pipeline-burst data generated from a random water supply network with theoretical hydraulic models to an actual imbalanced dataset. Accordingly, we established a machine learning model exploring a mapping matrix between two domains for minority-category data transfer. The experimental validation first verified the effectiveness and reliability of the proposed method between two customized water supply networks in terms of their bust recognition accuracy, model parameter sensitivity and time efficiency. Then, an actual monitoring dataset from a working water supply network was used to prove the suitability and compatibility of the proposed method. A bust-point location method was also provided based on the detection results of pipeline-bursting events. The validations show the superiority of our proposed approach for the imbalance data problem in pipe burst detection. [ABSTRACT FROM AUTHOR]

As a key step of tumor lymphatic metastasis, lymphangiogenesis is regulated by VEGFC‐VEGFR3 signaling pathway mediated by immune cells, mainly macrophages, in the tumor microenvironment. However, little is known whether tumor associated neutrophils are involved in lymphangiogenesis. Here, it is found that TANs infiltration is increased in LN‐metastatic BCa and is associated with poor prognosis. Neutrophil depletion results in significant reduction in popliteal LN metastasis and lymphangiogenesis. Mechanistically, transcription factor ETV4 enhances BCa cells‐derived CXCL1/8 to recruit TANs, leading to the increase of VEGFA and MMP9 from TANs, and then facilitating lymphangiogenesis and LN metastasis of BCa. Moreover, phosphorylation of ETV4 at tyrosine 392 by tyrosine kinase PTK6 increases nuclear translocation of ETV4 and is essential for its function in BCa. Overall, the findings reveal a novel mechanism of how tumor cells regulate TANs‐induced lymphangiogenesis and LN metastasis and identify ETV4 as a therapeutic target of LN metastasis in BCa. [ABSTRACT FROM AUTHOR]

The draining of brain interstitial fluid (ISF) to cerebrospinal fluid (CSF) and the subsequent draining of CSF to meningeal lymphatics is well‐known. Nonetheless, its role in the development of glioma is a remarkable finding that has to be extensively understood. The glymphatic system (GS) collects CSF from the subarachnoid space and brain ISF through aquaporin‐4 (AQP4) water channels. The glial limiting membrane and the perivascular astrocyte‐end‐feet membrane both have elevated levels of AQP4. CSF is thought to drain through the nerve sheaths of the olfactory and other cranial nerves as well as spinal meningeal lymphatics via dorsal or basal lymphatic vessels. Meningeal lymphatic vessels (MLVs) exist below the skull in the dorsal and basal regions. In this view, MLVs offer a pathway to drain macromolecules and traffic immunological cells from the CNS into cervical lymph nodes (CLNs), and thus can be used as a candidate curing strategy against glioma and other associated complications, such as neuro‐inflammation. Taken together, the lymphatic drainage system could provide a route or approach for drug targeting of glioma and other neurological conditions. Nevertheless, its pathophysiological role in glioma remains elusive, which needs extensive research. The current review aims to explore the lymphatic drainage system, its role in glioma progression, and possible therapeutic techniques that target MLVs in the CNS. [ABSTRACT FROM AUTHOR]

Background: To compare the diagnostic positive rate and complication rate between the electromagnetic navigation bronchoscopy (ENB) technique and computed tomography (CT)‐guided lung puncture for the biopsy of lung nodules located in the middle of an anatomic lung segment. Methods: Electronic medical records of 114 patients who underwent lung nodule biopsy between June 2021 and June 2022 were retrospectively evaluated. In all patients, the nodules were located in the middle third lung segment. To compare the diagnostic positive and complication rates between the two biopsy modalities performed in this lung region, clinical data, complication rates, nodule pathology, and imaging results were reviewed based on nodule characteristics retrieved from the electronic medical records. Results: Ninety‐three patients underwent CT‐guided lung puncture, while the remaining 21 patients underwent the ENB technique. No significant difference was observed in the diagnostic positive rate between the two groups (73.6 and 76.1%, respectively). In the CT‐guided lung puncture group, pneumothorax incidence, tube placement, postoperative hemorrhage, and symptomatic hemorrhage rates were 16.1, 6.5, 6.5, and 1.1%, respectively. In contrast, no complications occurred in the ENB group. Conclusions: The ENB technique is a safe and effective method for performing biopsies of pulmonary nodules with a diagnostic positive rate comparable to that of CT‐guided lung puncture and with a lower postoperative complication rate. [ABSTRACT FROM AUTHOR]

Active three-dimensional(3-D) thermography combines 3-D shape and active thermography. Thus, it enables the provision of the intuitive thermographic inspection results for composite parts with a complex geometry. However, conventional 3-D thermography acquires thermographic information and 3-D shape through at least two independent sensors and requires complex cross-modal image registration algorithm based on the keypoint detection and matching. In this article, an active 3-D thermography system is proposed using only one thermal camera for moving objects. This system does not require an independent 3-D sensor while the thermal camera acts as the 3-D sensor. Nature behind that is that a mathematical model is proposed to unify the line scanning thermography and laser triangulation in the dynamic scanning process, taking a line laser as both heat excitation for active thermography and spatial coding for 3-D reconstruction. Furthermore, the model enables the feature-free registration of thermogram sequence and 3-D shape, so that the registration is fast and robust without keypoint features. The experiments on standard height specimens, 3-D printing glass fiber composites, and carbon fiber intake tube have shown the error is calibrated within 0.25 mm in the range of 1 to 150 mm and evidenced the capability for subsurface defects detection. The ease and robustness of the proposed active 3-D thermography have a bright future for 3-D measurement, defects detection, and quality control in the production line. [ABSTRACT FROM AUTHOR]

Two improved analytical methods of calculations for natural frequencies and mode shapes of a uniform cantilever beam carrying a tip-mass under base excitation are presented based on forced vibration theory and the method of separation of variables, respectively. The cantilever model is simplified in detail by replacing the tip-mass with an equivalent inertial force and inertial moment acting at the free end of the cantilever based on D’Alembert’s principle. The concentrated equivalent inertial force and inertial moment are further represented as distributed loads using Dirac Delta Function. In this case, some typical natural frequencies and mode shapes of the cantilever model are calculated by the improved and unimproved analytical methods. The comparing results show that, after improvement, these two methods are in extremely good agreement with each other even the offset distance between the gravity center of the tip-mass and the attachment point is large. As further verification, the transient and steady displacement responses of the cantilever system under a sine base excitation are presented in which two improved methods are separately utilized. Finally, an experimental cantilever system is fabricated and the theoretical displacement responses are validated by the experimental measurements successfully.