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This study uses regional and global remote sensing data to assess the regrowth of secondary forests in the Brazilian Amazon biome. The authors find differences of regrowth rates due to climate, forest fires and deforestation actions and further quantify their carbon capture potential.

Autophagy must be tightly controlled at each step of the process. Here the authors show that HS1BP3 binds phosphatidic acid (PA) at autophagosome precursors and negatively regulates autophagosome formation by modulating the activity and localization of the PA-producing enzyme phospholipase D1.

Background: Androgen receptor (AR) and the phosphatidylinositol-3 kinase (PI3K) signaling are two of the most important pathways implicated in prostate cancer. Previous work has shown that there is crosstalk between these two pathways; however, there are conflicting findings and the molecular mechanisms are not clear. Here we studied the AR–PI3K pathway crosstalk in prostate cancer cells in vitro as well as in vivo.

Complex organ development depends on single lumen formation and its expansion during tubulogenesis. This can be achieved by correct mitotic spindle orientation during cell division, combined with luminal fluid filling that generates hydrostatic pressure. Using a human 3D cell culture model, we have identified two regulators of these processes. We find that pleckstrin homology leucine-rich repeat protein phosphatase (PHLPP) 2 regulates mitotic spindle orientation, and thereby midbody positioning and maintenance of a single lumen. Silencing the sole PHLPP family phosphatase in Drosophila melanogaster, phlpp, resulted in defective spindle orientation in Drosophila neuroblasts. Importantly, cystic fibrosis transmembrane conductance regulator (CFTR) is the main channel regulating fluid transport in this system, stimulated by phosphorylation by protein kinase A and inhibited by the AMP-activated protein kinase AMPK. During lumen expansion, CFTR remains open through the action of PHLPP1, which stops activated AMPK from inhibiting ion transport through CFTR. In the absence of PHLPP1, the restraint on AMPK activity is lost and this tips the balance in the favour of channel closing, resulting in the lack of lumen expansion and accumulation of mucus. [ABSTRACT FROM AUTHOR]

This study assessed the impact of dietary supplementation of single‐ and multi‐strain Bacillus‐based probiotics on growth performance, intestinal morphometry, blood parameters, immune‐oxidative status and transcriptomic response of Nile tilapia for 60 days. Fish (N = 150, 1.632 ± 0.035 g) were allocated into five experimental groups: (1) Control group (CG) that was fed on a basal diet; (2) Eco. 0.01%, received basal diet with 0.1 g/kg Ecobiol; (3) Eco. 0.02%, received basal diet with 0.2 g/kg Ecobiol; (4) Pro‐F FMC 0.001%, received basal diet with 0.001 g/kg Sanolife PRO‐F FMC; (5) Pro‐F FMC 0.002%, received basal diet with 0.002 g/kg Pro‐F‐FMC. Fish received Pro‐F FMC 0.002% displayed higher growth performance than CG (p < 0.05), while Pro‐F FMC 0.001% exhibited the highest intestinal weight. Sanolife PRO‐F FMC supplementation improved intestinal villi length and goblet cell number while decreased inter‐villi space (p < 0.05). The best haemoglobin, haematocrit and monocyte per cent were observed in Pro‐F FMC 0.001%, whereas Pro‐F FMC 0.002% revealed the best leukocyte, lymphocyte and neutrophil contents. Pro‐F FMC‐supplemented groups showed better phagocytic activity, phagocytic index and lysozyme activity than Ecobiol‐supplemented groups and CG. Pro‐F FMC 0.001% enrichment induced the highest upregulation level of NPYα, AgRp‐2, ghrelin and GH‐1, whereas Pro‐F FMC 0.002% significantly upregulated IGF‐1 and IGF‐2 gene expression. Eco. 0.02% induced the highest upregulation level of orexin and galanin gene expression. Conclusively, dietary enrichment with single‐ or multi‐strain Bacillus‐based probiotics is likely to improve growth, immunological parameters and transcriptomic response of Nile tilapia. [ABSTRACT FROM AUTHOR]

The present research was planned to assess the harmful effects of heavy metals as well as antioxidant alterations of Oreochromis niloticus collected from two polluted fish farms located in Abbassa and Manzala (areas A and B, respectively) in Egypt. They were compared with reference site in central laboratory for aquaculture researchers during the summer season, 2020. A total number of 75 fish collected from all sites was subjected to clinical, postmortem, and bacteriological examination. Water parameters, as well as heavy metals (arsenic, manganese, and nickel) in water, serum, and tissues (muscle, gills, and liver), were evaluated. In addition, antioxidant enzymes (SOD and CAT) and (MDA) were assessed in the serum of O. niloticus. Bacterial isolates, Aeromonas hydrophila, were identified in fish at both localities, and the recorded mortality rate was about 30% and 5% in areas A and B, respectively. The levels of As in water samples from both polluted areas were higher than the permissible limits. In addition, serum of O. niloticus showed a significant decrease in the concentrations of Ni in both polluted areas. In addition, a significant decrease in the mean levels of Ni and As was found in the gills and muscles of O. niloticus in both polluted areas. Moreover, a significant decrease in CAT, combined with a significant increase of SOD activities was observed in polluted area A. [ABSTRACT FROM AUTHOR]

Objectives. To describe methods employed to track infants enrolled in the New York State Zika Pregnancy and Infant Registry (NYSZPIR) and demonstrate the benefits of population databases to improve the process. Methods. We used patient medical records and provider outreach, New York State Immunization Information System (NYSIIS), and New York State Early Hearing Detection and Intervention Information System (NYEHDI-IS) to gather medical information. We used descriptive statistics to summarize variables and the McNemar test to determine statistical significance (P <.05). Results. We identified 109 live births from NYSZPIR mothers. Provider information was documented for 106 (97.2%) infants in NYSIIS compared with 72 (66.1%) through chart review. Collected results of newborn hearing screening increased from 82 (75.2%) to 106 (97.2%) using NYEHDI-IS. The amount of data obtained was significantly higher (P <.001) when including NYSIIS and NYEHDI-IS compared with using medical records alone. Conclusions. Public health surveillance systems can be used to track infants using data sources such as NYSIIS and NYEHDI-IS in addition to traditional methods. Using medical records alone is inadequate for locating and tracking infants and may result in high lost to follow-up rates. [ABSTRACT FROM AUTHOR]

One of the top challenges in education and neuroscience consists in translating laboratory results into strategies to improve learning and memory in teaching environments. In that sense, during the last two decades, researchers have discovered specific temporal windows around learning, during which the intervention with some experiences induces modulatory effects on the formation and/or persistence of memory. Based on these results, the aim of the present study was to design a specific strategy to improve the memory of students in a high-school scenario, by assessing the effect of a novel situation experienced close to learning. We found that the long-term memory about a geometrical figure was more precise in the group of students that faced a novel situation 1 h before or after learning the figure than the control group of students who did not face the novelty. This enhancement was probably triggered by processes acting on memory formation mechanisms that remained evident 45 days after learning, indicating that the improvement was sustained over time. In addition, our results showed that novelty no longer improved the memory if it was experienced 4 h before or after learning. However, far beyond this window of efficacy, when it was faced around 10 h after learning, the novel experience improved the memory persistence tested 7 days later. In summary, our findings characterized different temporal windows of the effectiveness of novelty acting on memory processing, providing a simple and inexpensive strategy that could be used to improve memory formation and persistence in high-school students. [ABSTRACT FROM AUTHOR]

Wind energy generation has become an important means to reduce reliance on fossil fuels and mitigate against human‐induced climate change, but could also represent a significant human–wildlife conflict. Airborne taxa such as birds may be particularly sensitive to collision mortality with wind turbines, yet the relative vulnerability of species' populations across their annual life cycles has not been evaluated.Using GPS telemetry, we studied the movements of lesser black‐backed gulls Larus fuscus from three UK breeding colonies through their annual cycle. We modelled the distance travelled by birds at altitudes between the minimum and maximum rotor sweep zone of turbines, combined with the probability of collision, to estimate sensitivity to collision. Sensitivity was then combined with turbine density (exposure) to evaluate spatio‐temporal vulnerability.Sensitivity was highest near to colonies during the breeding season, where a greater distance travelled by birds was in concentrated areas where they were exposed to turbines.Consequently, vulnerability was high near to colonies but was also high at some migration bottlenecks and wintering sites where, despite a reduced sensitivity, exposure to turbines was greatest.Synthesis and applications. Our framework combines bird‐borne telemetry and spatial data on the location of wind turbines to identify potential areas of conflict for migratory populations throughout their annual cycle. This approach can aid the wind farm planning process by: (a) providing sensitivity maps to inform wind farm placement, helping minimize impacts; (b) identifying areas of high vulnerability where mitigation warrants exploration; (c) highlighting potential cumulative impacts of developments over international boundaries and (d) informing the conservation status of species at protected sites. Our methods can identify pressures and linkages for populations using effect‐specific metrics that are transferable and could help resolve other human–wildlife conflicts. [ABSTRACT FROM AUTHOR]

The biological effects of gamma radiation may exert damage beyond that of the individual through its deleterious effects on reproductive function. Impaired reproductive performance can result in reduced population size over consecutive generations. In a continued effort to investigate reproductive and heritable effects of ionizing radiation, we recently demonstrated adverse effects and genomic instability in progeny of parents exposed to gamma radiation. In the present study, genotoxicity and effects on the reproduction following subchronic exposure during a gametogenesis cycle to 60 Co gamma radiation (27 days, 8.7 and 53 mGy/h, total doses 5.2 and 31 Gy) were investigated in the adult wild-type zebrafish ( Danio rerio ). A significant reduction in embryo production was observed one month after exposure in the 53 mGy/h exposure group compared to control and 8.7 mGy/h. One year later, embryo production was significantly lower in the 53 mGy/h group compared only to control, with observed sterility, accompanied by a regression of reproductive organs in 100% of the fish 1.5 years after exposure. Histopathological examinations revealed no significant changes in the testis in the 8.7 mGy/h group, while in 62.5% of females exposed to this dose rate the oogenesis was found to be only at the early previtellogenic stage. The DNA damage determined in whole blood, 1.5 years after irradiation, using a high throughput Comet assay, was significantly higher in the exposed groups (1.2 and 3-fold increase in 8.7 and 53 mGy/h females respectively; 3-fold and 2-fold increase in 8.7 and 53 mGy/h males respectively) compared to controls. A significantly higher number of micronuclei (4–5%) was found in erythrocytes of both the 8.7 and 53 mGy/h fish compared to controls. This study shows that gamma radiation at a dose rate of ≥ 8.7 mGy/h during gametogenesis causes adverse reproductive effects and persistent genotoxicity (DNA damage and increased micronuclei) in adult zebrafish. [ABSTRACT FROM AUTHOR]

Recent developments in GPS tracking technology allow the movements of bird species to be followed in ever-greater detail. Seabird research is benefiting greatly, due to the challenges of tracking species that often roam widely out at sea. Amongst the gulls, one of the pressing issues is to understand the ecology of the relatively recent urban colonists and how they differ from their counterparts in traditional rural colonies. Here, we present what we believe are the first GPS results from roof-nesting gulls. Four adult Herring Gulls (two males, two females) were fitted with GPS tags in May 2014 in the seaside town of St Ives, Cornwall (breeding colony c 250 pairs), and tracked for c 100 days during the 2014 breeding season. We estimated the home ranges of the four individuals and how their movement behaviour varied through the 24-h period and across the breeding season. The results highlight how variable movement behaviour was among individuals: whilst one bird roamed widely (90% range estimate = 560 km2), heading >50 km offshore and often active at night or roosting at sea, two birds had small ranges (<10 km2), always attended the colony at night and rarely headed more than a few hundred metres offshore, with the fourth displaying intermediate behaviour. All of the birds regularly utilised a few key sites within the agricultural landscape south of St Ives. Whilst this study was too small to allow general conclusions to be drawn about urban Herring Gulls, it reinforces how variable individual behaviour can be amongst the large gulls and will be particularly interesting when applied to a larger sample of birds, especially in big urban gull colonies further inland. [ABSTRACT FROM AUTHOR]

Telemetry has become an important method for studying the biology and ecology of animals. However, the impact of tracking devices and their method of attachment on different species across multiple temporal scales has seldom been assessed. We compared the behavioural and demographic responses of two species of seabird, Lesser Black-backed Gull Larus fuscus and Great Skua Stercorarius skua, to a GPS device attached using a crossover wing harness. We used telemetry information and monitoring of breeding colonies to compare birds equipped with a device and harness, and control birds without an attachment. We assessed whether tagged birds have lower short-term breeding productivity or lower longer term overwinter return rates (indicative of overwinter survival) than controls. For Great Skua, we also assessed whether territory attendance within the breeding season differed between tagged and control birds. As with previous studies on Lesser Black-backed Gull, we found no short-term impacts on breeding productivity or long-term impacts on overwinter return rates. For Great Skua, there was no evidence for impacts of the device and harness on territory attendance or breeding productivity. However, as found by a previous study of Great Skuas using a different (body) harness design, there was strong evidence of reduced overwinter return rates. Consequently, a device attached using a wing harness was considered suitable for long-term deployment on Lesser Black-backed Gulls, but not on Great Skuas. These findings will inform the planning of future tracking studies. [ABSTRACT FROM AUTHOR]

The marine environment is increasingly pressured from human activities, such as offshore renewable energy developments. Offshore wind farms may pose direct risks to seabirds at protected breeding sites. However, changes in food availability may influence foraging behaviour and habitat use during the breeding season or between years. Consequently, seabird–wind farm interactions, and risks posed to populations, may vary over longer time scales, but this has seldom been quantified. We used GPS-telemetry to study the movements of 25 lesser black-backed gulls from the Alde–Ore Special Protection Area (SPA), UK between 2010 and 2012, while birds were associated with their breeding colony. Variation in movements away from the colony, offshore, and in operational, consented and proposed Offshore Wind Farm Areas (“OWFAs”) was investigated: (1) between years and (2) across the breeding season, addressing: (3) sex-specific, (4) individual and (5) diurnal/nocturnal differences. The extent of overlaps with OWFAs varied between years, being greatest in 2010 (7/10 birds showing connectivity; area overlap: 6.2 ± 7.1%; time budget overlap: 4.6 ± 6.2%) and least in 2012. Marine habitats close to the colony were used before breeding. Birds spent little time offshore as incubation commenced, but offshore usage again peaked during the early chick-rearing period, corresponding with use of OWFAs. Individuals differed in their seasonal interactions with OWFAs between years, and males used OWFAs significantly more than females later in the breeding season. This study demonstrates the importance of tracking animals over longer periods, without which impact assessments may incorrectly estimate the magnitude of risks posed to protected populations. [ABSTRACT FROM AUTHOR]

Tracking devices are useful in studying the movement and behaviour of birds throughout the year. However, the effects of device attachment must be monitored for bird welfare and the scientific veracity of information gathered. We report on a trial of three different harness types (leg-loop, body and wing) for attaching GPS devices to Lesser Black-backed Gulls Larus fuscus and of a leg-loop harness on Great Skuas Stercorarius skua. This trial evaluated the functionality of the device whilst deployed, harness retention and the effects of each harness on the birds. The leg-loop harness prevented the devices from charging, due to feathers overlapping their solar panels, and individuals of both species shed this attachment during the first breeding season. For Lesser Black-backed Gull, devices functioned properly when attached using body and wing harnesses; foraging behaviour and territory attendance of birds were similar to the findings of other studies. Despite small sample sizes, annual adult survival rates for Lesser Black-backed Gull were similar to published estimates, suggesting harnesses had no deleterious effects. The wing harness has a smaller amount of skin contact than the body harness, and a non-constricting fit that better accommodates changes in body size. The wing harness was therefore considered the most appropriate for long-term studies. [ABSTRACT FROM AUTHOR]

Previously, we have shown that the phosphoinositidemetabolizing enzymes PIKfyve (phosphoinositide 5-kinase, FYVE finger containing) and MTMR3 (myotubularin-related protein 3), together with their lipid product PtdIns5P, are important for migration of normal human fibroblasts. As these proteins are a kinase and a phosphatase respectively, and thereby considered druggable, we wanted to test their involvement in cancer cell migration and invasion. First, we showed that PIKfyve and MTMR3 are expressed in most cancer cells. Next, we demonstrated that depletion of PIKfyve or MTMR3 resulted in decreased velocity in three different cancer cell lines by using new software for cell tracking. Inhibition of the enzymatic activity of PIKfyve by the inhibitor YM201636 also led to a strong reduction in cell velocity. Mechanistically, we show that PIKfyve and MTMR3 regulate the activation of the Rho family GTPase Rac1. Further experiments also implicated PtdIns5P in the activation of Rac1. The results suggest a model for the activation of Rac1 in cell migration where PIKfyve and MTMR3 produce PtdIns5P on cellular membranes which may then serve to recruit effectors to activate Rac1. Finally, in an invasion assay, we demonstrate that both PIKfyve and MTMR3 are implicated in invasive behaviour of cancer cells. Thus PIKfyve andMTMR3 could represent novel therapeutic targets in metastatic cancer. [ABSTRACT FROM AUTHOR]