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Isotretinoin is an efficacious treatment option for severe acne. Although isotretinoin often causes mild liver enzyme elevation, how acne patients should be monitored on isotretinoin therapy is not well characterized. The purpose of this study was to assess the management and clinical outcome of acne patients with abnormal aspartate transaminase (AST) and alanine aminotransferase (ALT) when receiving isotretinoin. A retrospective chart review was conducted in acne subjects with abnormal AST and ALT levels receiving isotretinoin. Abnormal liver enzymes were graded using the Common Terminology Criteria for Adverse Events version 5. Of 108 subjects with abnormal liver enzymes, 79 subjects were on isotretinoin 80 mg and 23 subjects were on isotretinoin 40 mg. Most abnormalities were during Month 1 of therapy (48). Of the 122 abnormal Grade 1 AST elevations, 40 normalized, 38 remained in Grade 1, and 1 increased into Grade 2 when a healthcare provider maintained the isotretinoin dose. Of the 102 abnormal Grade 1 ALT levels managed by maintaining the isotretinoin dose, 31 normalized and 38 remained persistently elevated. Most mild elevations of isotretinoin therapy do not worsen. Acne patients with isotretinoin may not need continued testing when experiencing low-grade liver enzyme abnormalities. [ABSTRACT FROM AUTHOR]

Patients with major burn injuries typically require numerous blood transfusions. It is not known if an inhalation injury (INHI) directly influences the need for blood transfusion. The purpose of this study was to determine whether INHI increases the amount of blood transfused to major burn patients. A secondary analysis from the Transfusion Requirement in Burn Care Evaluation (TRIBE) study was conducted. Patients with INHI were compared with patients without INHI. The number of red blood cell (RBC) transfusions per day (RBC per day) between INHI and No INHI was analyzed with a multivariable regression. Patients with INHI (n = 78) had significantly larger burns (P = .0004), larger full-thickness burns (P = .0007), greater admission APACHE score (P < .0001), higher admission multiple organ dysfunction scores (P < .0001), and were transfused more RBC per day (P = .009) than No INHI patients (n = 267). In the multivariable regression analysis, RBC per day was significantly associated with the %TBSA burn (P < .0001), age of the patient (P = .004), the need for more than 1 day of mechanical ventilation (P < .0001), the occurrence of at least one blood stream infection (BSI; P = .044), and being assigned to the liberal transfusion arm of TRIBE (P < .001) but not the presence of INHI (P = .056). The null hypothesis that INHI exerts no influence on the amount of blood transfused could not be rejected. Larger burn size, advanced patient age, mechanical ventilation, and BSIs are important determinants of the blood transfusion rate in major burn patients. [ABSTRACT FROM AUTHOR]

The article presents a study which examines the differences in perceptions of consumers about food allergen thresholds and precautionary allergen labeling (PAL) in countries including Chile, France, and U.S. An online survey was administered by Survey Monkey and chi-square tests were utilized. It shows that opinions on the purchase of packaged foods with precautionary label of possible allergen were similar.

Significant In-Band Stray Light (IBSL) error at solar zenith angle (SZA) values larger than 77° near sunset in 4 SBUV/2 instruments has been characterized. The IBSL error is caused by large surface reflection and scattering of the air-gapped depolarizer in front of the instrument's monochromator aperture. The source of the IBSL error is direct solar illumination of instrument components near the aperture rather than from earth shine. We have analyzed SBUV/2 albedo measurements on both dayside and night side to develop an empirical model for the IBSL error. This error has been corrected in the V8.6 SBUV/2 ozone retrieval. [ABSTRACT FROM AUTHOR]

We present validation of ozone profiles from a number of Solar Backscatter Ultraviolet (SBUV and SBUV/2) instruments that were recently reprocessed using an updated (Version 8.6) algorithm. The SBUV data record spans a 41-yr period from 1970 to 2011 with a 5-yr gap in the 1970s. The ultimate goal is to create a consistent, well-calibrated dataset of ozone profiles that can be used for climate studies and trend analyses. SBUV ozone profiles have been intensively validated against satellite profile measurements from the Microwave Limb Sounders (MLS) (on board UARS and Aura satellites) and the Stratospheric Aerosol and Gas Experiment (SAGE II) and ground-based observations from the microwave spectrometers, lidars, Umkehr instruments and balloon-borne ozonesondes. In the stratosphere between 25 and 1 hPa the mean biases and standard deviations are mostly within 5% for monthly zonal mean ozone profiles. Above and below this layer the vertical resolution of the SBUV algorithm decreases. We combine several layers of data in the troposphere/lower stratosphere to account for the lower resolution. The bias in the SBUV tropospheric/lower stratospheric combined layer relative to similarly integrated columns from Aura MLS, ozonesonde and Umkehr instruments varies within 5 %. We also estimate drifts in the SBUV instruments and their potential effect on the long-term stability of the combined data record. Data from the SBUV instruments that collectively cover the 1980s and 2000s are very stable, with drifts mostly less than 0.5%yr-1. The features of individual SBUV(/2) instruments are discussed and recommendations for creating the merged SBUV data set are provided. [ABSTRACT FROM AUTHOR]

The article discusses a study that describes the calibration process for the Solar Backscatter Ultraviolet (SBUV) Version 8.6 (V8.6) ozone data product. It shows that they primary SBUV instrument characterization is based on prelaunch laboratory tests and dedicated on-orbit calibration measurements. It determines the V8.6 calibration adjustments are determined at the radiance level.

Globozoospermia is an infrequent pathology in which spermatozoa tack acrosomes. Patients are considered sterile without IVF augmented with intracytoplasmic sperm injection (ICSI), as fertilization is impaired due to absence of oocyte activation. As far as is known, this is the first study to report results of a comprehensive approach to the treatment of the semen parameters, sperm DNA fragmentation, aneuploidy, transmission electron microscopy, Western blotting and immunofluorescence for detection of phospholipase C zeta (PLCζ), as well as ICSI outcome, of an affected patient. Morphological evaluation and transmission electron microscopy revealed complete globozoospermia with significant duplicate heads and tails. Analysis for DNA damage revealed fragmentation rates of approximately 80% in semen and 15-23% in swim-up fractions. PLCζ was not detected by immunofluorescence or Western blotting. Aneuploidy rates were within normal ranges. ICSI followed by oocyte activation with calcium ionophore resulted in high rates of fertilization, and an ongoing pregnancy was established after transfer of cryopreserved-thawed embryos. [ABSTRACT FROM AUTHOR]

Lupine has been increasingly used in food applications due to its high nutritional value and excellent functional properties. However, lupine provokes allergic reactions in susceptible individuals. The presence of undeclared lupine residues in foods can pose a serious health risk to lupine-allergic individuals. Therefore, the objective of this research was to develop a sandwich-type ELISA for the detection of lupine residues in foods. Lupine flour derived from Lupinus albus was used to immunize 3 rabbits and a sheep. Pooled lupine-specific antibodies were partially purified from the sera by ammonium sulfate precipitation. A sandwich lupine ELISA with a limit of quantification (LOQ) of 1 ppm was developed by utilizing the rabbit antisera as the capture reagent and the sheep antiserum as the detector reagent. The binding of the antigen-antibody complex was visualized by the addition of commercial rabbit antisheep IgG antibody labeled with alkaline phosphatase with subsequent addition of p-nitrophenyl phosphate substrate to produce a colored product for quantification. Minor cross-reactivity was observed with soy ( Glycine max) and black bean ( Castanospermum australe). The performance of the lupine ELISA was evaluated in reference food standards (beef frankfurter and apple cinnamon muffin) and laboratory-prepared cooked frankfurters and corn muffins. The mean percent recovery for lupine spiked-frankfurters and corn muffins were 108.4%± 8.8% and 103.1%± 11.5%, respectively. The sandwich-type lupine ELISA developed in this study provides food manufacturers and regulatory agencies with an effective analytical tool to detect and quantify lupine residues in processed foods. [ABSTRACT FROM AUTHOR]

Undeclared mustard residues in food products could trigger allergic reactions in mustard-allergic consumers. Our objective was to develop and validate a sandwich-type ELISA for the detection of mustard residues in foods. A mixture of yellow, brown, and oriental mustard seeds was used to immunize 3 rabbits and 1 sheep. Two mustard ELISAs were developed by utilizing the reciprocal combination of rabbit and sheep polyclonal antimustard sera as the capture and detector reagents. Binding was visualized by addition of rabbit antisheep or goat antirabbit IgG antibody labeled with alkaline phosphatase and subsequent addition of substrate. The optimized ELISAs have limits of quantification (LOQ) of 1 and 3 ppm (μg of ground, whole mustard seeds/mL) for the sheep capture and rabbit capture formats, respectively. Only rapeseed cross-reacted in the rabbit and sheep capture mustard ELISAs at a level equivalent to 12300 and 16900 ppm of mustard. The mean percent recovery for cooked frankfurters spiked with 0 to 1000 ppm mustard flour was 95.3%± 10.7%. A limited retail survey of 29 foods revealed that, of 15 samples having mustard declared on the ingredient list, 2 baked bean products contained no detectable mustard, possibly owing to a decrease in extractability and detectability of mustard proteins after subjecting to thermal processing. For the remaining 14 samples without mustard declared on the label, 3 samples contained detectable mustard, presumably due to the labeling of mustard as “spice” or inadvertent cross-contamination. This sandwich-type ELISA can serve as a powerful tool for food manufacturers and regulatory agencies to detect and quantify mustard residues in processed foods. [ABSTRACT FROM AUTHOR]

The incidence of human non-typhoidalSalmonella(NTS) infection has increased in many countries. Endovascular infection is one of the most serious forms of extraintestinal infection. Five patients with NTS endovascular infection treated at Middlemore Hospital, Auckland, New Zealand, are presented here. All persons with NTS bacteraemia who are older than 50 years and have a risk of atherosclerosis should be evaluated for possible endovascular infection. (Intern Med J 2004; 34: 641−645) [ABSTRACT FROM AUTHOR]

The influence of deep-frying using different oils and temperatures on carotenoid content and physicochemical and sensory characteristics of carrot chips was investigated. Sliced carrots were steam-blanched, cooled, soaked in 0.2% sodium metabisulfite, and deep-fried in canola, palm, or partially hydrogenated soybean oil (PHSO) at 165, 175, or 185 °C. Frying temperature, but not oil, significantly (P < 0.05) affected the α- carotene, β-carotene, and total carotenoid contents. Off type significantly (P < 0.05) influenced all color values. Increasing temperature lowered the redness value, which correlated with decreased carotenoid content, color darkening, and decreased hardness value. Trained panelists detected no differences among oil types in crispness, sweetness, odor, and acceptability. The best carrot-chip product was that fried in PHSO at 165 °C. [ABSTRACT FROM AUTHOR]

Large coefficients of variation (>30%) are often associated with increased experimental variability. The objective of this study was to examine the relationships between mean square errors, yield means, coefficients of variation (CV), and plot size using statistical information compiled from past wheat field research projects. Three hundred and sixty two wheat field research projects were selected from over 2,000 published wheat experiments that included soil fertility, weed, tillage, and variety evaluation. Little or no relationship between mean square error (MSE) and mean yield or plot size was found. However, mean yields and CVs demonstrated a significant negative correlation. This work proposes decreased variability among experimental units as defined by the CV can be accomplished simply by increasing the mean yield, suggesting that the use of the CV may be improper when comparing variability of trials from similar experiments. Attempts to compare variation from similar experiments should be done using the MSE since unit differences would not be a problem. The CV should only be used when comparing variation from experiments using different metrics. Plot size in plant breeding variety trials (average of 3.59 m2) was much smaller than that in fertilizer/weed/tillage trials (average of 37.2 m2). The smaller plot size employed in the variety trials reduced the variability encountered in the estimation of the mean and is consistent with the resolution where detectable differences in soil test parameters exist and that should be treated independently. [ABSTRACT FROM PUBLISHER]