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In advanced stages of in Huntington's disease (HD) gait impairments and severe chorea are usually medication-refractory. The long-term effects on gait in HD of physiotherapy ICF-based management post- globus pallidus deep brain stimulation (GPi DBS) are not well-established. Physiotherapy has been recognized as an essential element in HD treatment. Here, we present a case report of a 56-year-old woman with HD on the advanced stage and severe chorea medication-refractory after GPi-DBS. We performed multidisciplinary motor assessments ICF-based to identify the disability at clinical and home-setting, including environmental and personal factors before and after GPi-DBS surgery and at 11-time points follow-up. The surgery was very successful and directly post GPi-DBS, there were a significant improvement in chorea and a substantial decrease in medication dose. A framework ICF- based physiotherapy protocol with external cues was developed to improve gait was delivered post-surgery and was continued three times/week during 18-months. Physiotherapy sessions consisted of a personalized protocol of exercises with functional movements, balance, and gait training with external cues. Improvements in gait were observed in 3-months post-intervention and were more expressive in 6-months follow-up. Our patient improved substantially HD motor symptoms and her quality of life after GPi-DBS intervention and a physiotherapy program ICF-based. The objective outcomes measures used to assess gait have served as endpoints to assessing the patient's motor profile during the pre-operative period. Assessments were helpful to verify the efficacy of the multidisciplinary intervention in long-term.ConclusionPeriodically assessing function and disability using outcome improvements may support clinicians' decisions about DBS, medication adjustments and guide physiotherapists to personalize the ICF-based intervention.

Immune-compromised mouse models allow for testing the preclinical efficacy of human cell transplantations and gene therapy strategies before moving forward to clinical trials. However, CRISPR/Cas9 gene editing of the Wsh/Wsh mouse strain to create an immune-compromised model lacking function of Rag2 and Il2rγ led to unexpected morbidity and mortality. This warranted an investigation to ascertain the cause and predisposing factors associated with the outbreak. Postmortem examination was performed on 15 moribund mice. The main lesions observed in these mice consisted of ascending urogenital tract infections, suppurative otitis media, pneumonia, myocarditis, and meningoencephalomyelitis. As Escherichia coli strains harboring polyketide synthase (pks) genomic island were recently isolated from laboratory mice, the tissue sections from the urogenital tract, heart, and middle ear were subjected to E. coli specific PNA-FISH assay that revealed discrete colonies of E. coli associated with the lesions. Microbiological examination and 16S rRNA sequencing confirmed E. coli-induced infection and septicemia in the affected mice. Further characterization by clb gene analysis and colibactin toxicity assays of the pks+ E. coli revealed colibactin-associated cytotoxicity. Rederivation of the transgenic mice using embryo transfer produced mice with an intestinal flora devoid of pks+ E. coli. Importantly, these barrier-maintained rederived mice have produced multiple litters without adverse health effects. This report is the first to describe acute morbidity and mortality associated with pks+ E. coli urosepsis and meningitis in immunocompromised mice, and highlights the importance of monitoring and exclusion of colibactin-producing pks+ E. coli.

New formulations for the viscosity and thermal conductivity for nitrogen, oxygen, argon, and air are given. Air is treated as a pseudo-pure fluid using an approach adopted from previous research on the equation of state for air. The equations are valid over all liquid and vapor states, and a simplified cross-over equation was used to model the behavior of the critical enhancement for thermal conductivity. The extrapolation behavior of the equations for nitrogen and argon well below their triple points was monitored so that both could be used as reference equations for extended corresponding states applications. The uncertainties of calculated values from the equations are generally within 2% for nitrogen and argon and within 5% for oxygen and air, except in the critical region where the uncertainties are higher. Comparisons with the available experimental data are given. [ABSTRACT FROM AUTHOR]

Changes in the intensity of repeated, ignored sounds elicit the mismatch negativity (MMN) brain response which reflects preattentive detection of the change. It is generally assumed that the MMN in response to intensity changes reflects a memory-based comparison mechanism rather than being due to differential states of refractoriness of intensity-specific cortical neurons. In the present study, an experimental protocol consisting of 4 oddball blocks and 1 control block was used in order to separate memory-comparison-related effects from refractoriness-related ones. This design allowed an assessment of intensity MMN using physically identical stimuli with equal probability of occurrence in separate blocks, while avoiding contamination by refractoriness. Results were consistent with an MMN in response to intensity change that reflects genuine memory-based comparison.Copyright © 2003 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]

Purpose: Research supports physical activity as a method to heighten stress resistance and resilience through positive metabolic alterations mostly affecting the neuroendocrine system. High-intensity interval training (HIIT) has been proposed as a highly effective time-saving method to induce those changes. However, existing literature relies heavily on cross-sectional analyses, with few randomised controlled trials highlighting the necessity for more exercise interventions. Thus, this study aims to investigate the effects of HIIT versus an active control group on the stress response to an acute psychosocial stressor in emotionally impulsive humans (suggested as being strong stress responders)., Methods: The study protocol was registered online (DRKS00016589) before data collection. Sedentary, emotionally impulsive adults (30.69 ± 8.20 y) were recruited for a supervised intervention of 8 weeks and randomly allocated to either a HIIT (n = 25) or a stretching group (n = 19, acting as active controls). Participants were submitted to a test battery, including saliva samples, questionnaires (self-efficacy- and perceived stress-related), visual analogue scales (physical exercise- and stress-related), and resting electroencephalography and electrocardiography assessing their reaction to an acute psychological stressor (Trier Social Stress Test) before and after the exercise intervention., Results: HIIT increased aerobic fitness in all participants, whereas stretching did not. Participants from the HIIT group reported perceiving exercising more intensively than those from the active control group (ƞ p 2  = 0.108, p = 0.038). No further group differences were detected. Both interventions largely increased levels of joy post-TSST (ƞ p 2  = 0.209, p = 0.003) whilst decreasing tension (ƞ p 2  = 0.262, p < 0.001) and worries (ƞ p 2  = 0.113, p = 0.037). Finally, both interventions largely increased perceived levels of general self-efficacy (ƞ p 2  = 0.120, p = 0.029)., Conclusion: This study suggests that 8 weeks of HIIT does not change the psychoneuroendocrine response to an acute psychological stress test compared to an active control group in emotionally impulsive humans. Further replications of supervised exercise studies highly powered with active and passive controls are warranted., (© 2024. The Author(s).)

Affective responses can influence evaluative judgments, but how are subjective beauty ratings affected by references to morally contentious elements in aesthetic stimuli? In an online experiment (N = 460), we investigated the relationship between two types of descriptive texts (Neutral vs. Negative) and the beauty ratings of 25 photographs that depict sources of environmental pollution. For each photograph, the neutral descriptive text contained general information, whereas the negative descriptive text addressed the pollution source. Further, we explored whether this relationship is mediated by changes in positive and negative affect, and how it interacts with the biospheric values of participants. Our results showed that (1) participants in the Negative Condition rated the photographs as less beautiful than in the Neutral Condition, (2) this relationship was partially mediated by changes in negative affect, and (3) in the Negative Condition, participants with higher levels of biospheric values rated the photographs as less beautiful. Our results indicate that individual values, as well as affective responses induced by aesthetic stimuli, directly influence subjective beauty. This aligns with current theoretical frameworks and fills a gap in experimental research. Finally, we discuss limitations and directions for future studies. PSYCHINFO CLASSIFICATION CODE: 2340., Competing Interests: Declaration of competing interest Regarding all aspects of this study, all authors confirm that they have no conflicts of interest to disclose., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Objectives: Individuals vary in their levels of Desire for Aesthetics, ranging from superficial interest to profound immersion. However, there is currently a lack of measurement tools available to assess Desire for Aesthetics across various domains within German-speaking populations. Addressing this gap, the present study aimed to develop the Desire for Aesthetics Scale in German (DFAS-G) and evaluate its psychometric properties to comprehensively assess aesthetic preferences and inclinations., Methods: We conducted three consecutive studies and collected three samples of students and university employees. In Study 1 (N = 317), we selected a translated item pool using the forward-backward translation method, conducted cultural adaptation of the items, and assessed the scale's factor structure through an exploratory factor analysis. In Study 2 (N = 304), we revised and cross-validated the scale using confirmatory factor analysis and examined the final 37-item DFAS-G for reliability and validity. In Study 3 (N = 300), we further examined the scales construct validity and assessed retest reliability 291 days after initial testing., Results: The factor analysis conducted in Study 1 resulted in a four-factor solution representing the Desire for Aesthetics in four domains: Visual Arts, Individuals, Music, and Habitat. We found good internal consistency for the global scale (Cronbach's alpha = 0.87) and all subscales (0.74 to 0.82). We also found construct validity with Art Interest and facets of the Big Five personality trait Openness and provided first evidence of criterion validity and incremental validity of the DFAS-G global score toward Aesthetic Perception and Visual Arts toward Art Interest in Study 2. We further found evidence of convergent validity with the Openness facet Aesthetics, discriminant validity with the facet Values, and high test-retest reliability (0.85 to 1.00) in Study 3., Conclusions: The present article provides a scale for measuring DfA in German-speaking populations and first steps of its validation. The DFAS-G extends the repertoire of options for psychological research on individual differences in aesthetics appreciation. Follow-up studies could provide further evidence of the subscale's validity and equivalence to the original scale., Competing Interests: Declaration of competing interest The authors have no known conflict of interest to disclose. This research is covered by our general ethical vote., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Acute stress is assumed to affect executive processing of stimulus information, although extant studies have yielded heterogeneous findings. The temporal flanker task, in which a target stimulus is preceded by a distractor of varying utility, offers a means of investigating various components involved in the adjustment of information processing and conflict control. Both behavioral and EEG data obtained with this task suggest stronger distractor-related response activation in conditions associated with higher predictivity of the distractor for the upcoming target. In two experiments we investigated distractor-related processing and conflict control after inducing acute stress (Trier Social Stress Test). Although the stressed groups did not differ significantly from unstressed control groups concerning behavioral markers of attentional adjustment (i.e., Proportion Congruent Effect), or event-related sensory components in the EEG (i.e., posterior P1 and N1), the lateralized readiness potential demonstrated reduced activation evoked by (predictive) distractor information under stress. Our results suggest flexible adjustment of attention under stress but hint at decreased usage of nominally irrelevant stimulus information for biasing response selection., (© 2024. The Author(s).)

Attention can be directed to the global or local level of a visual stimulus (i.e., Navon figure). Previous studies yielded reliable trial-to-trial level switch costs (i.e., worse performance when responding to the other level than on a previous trial), even though level cueing effects indicated anticipatory deployment of attention to the upcoming target level. To investigate the interplay of attentional preparation and persistence, we applied a probe trial method assumed to ensure a high degree of preparation for the upcoming target level and minimizing stimulus-specific proactive interference. Mirroring previous findings obtained in the domain of spatial attention, we found evidence for anticipatory attentional focusing on global/local target levels but not for persistence of the attentional set adopted on the previous trial. In a second experiment, we prevented preparation for upcoming attentional demands (in both global-local and spatial attention tasks). This resulted in the modulation of performance (in critical probe trials) by the attentional demands of the predecessor trial. Together, our findings demonstrate sensitivity of the probe trial method for attentional persistence and raise the possibility that such persistence can be completely eliminated by sufficiently strong preparation for the attentional demands of the following trial., Competing Interests: Declaration of competing interest None., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Beauty surrounds us in many ways every day. In three experience sampling (ESM) studies we investigated frequency, category of eliciting stimuli (natural vs human-made) and, the potential moderating role of several individual difference measures on such everyday experiences of beauty in an ecologically valid manner. Further, we explored the impact of such experiences on valence & arousal. Study 1 re-analysed data from a previous study, in line with the current aims. In Studies 2 and 3, we asked participants to report daily experiences of beauty using a mixed random and event-contingent sampling schedule. Mobile notifications (random sampling) prompted participants to take a photo and rate the beauty of their surroundings. Further, current valence and arousal were assessed. Notification frequency and total days of participation differed between these two studies. Participants were able to report additional experiences outside of the notification windows (event-contingent sampling). Our results indicate that we frequently encounter beauty in everyday life and that we find it in nature, in particular. Our results further suggest a mood-boosting effect of encounters with beauty. Lastly, our results indicate influences of individual differences however, these were inconclusive and require further attention., (© 2024. The Author(s).)

The prevalence of undocumented medical treatments among children is a significant issue, as well as many EU countries lack access to newly developed children-friendly medicines. Consequently, there is a pressing need for supplementary resources that can facilitate informed decision-making regarding children's medication. We therefore aim to describe the process of establishing a children's Drug and Therapeutics Committee (cDTC), as well as the preparing and implementation of recommendations for children in the capital region of Denmark. Following the guidelines outlined by the World Health Organization, we established a cDTC, and recommendations for paediatric medication practice were constructed from assessments of medication use patterns among children in the capital region between 2019 and 2021. The recommendations were meticulously crafted based on evaluation of the current marketing authorization landscape and existing best available evidence. In 2019, the capital region established the first cDTC supported by expert councils and an editorial board. A total of 2429 purchase item numbers covering 1 222 846 defined daily doses and 592 088 purchased packages covering 10 200 000 defined daily doses were identified in the secondary and primary sectors, respectively. Three comprehensive lists covering recommendations for newborns and children were published between 2021 and 2020 totaling 331 recommended pharmaceutical products. The recommendations primarily intended for use in the secondary healthcare sector were implemented through the revision of 38 paediatric- and six neonatal product ranges throughout capital region. In conclusion, recommendation lists for children governed by a cDTC provide a rational auxiliary tool that can be immediately implemented in the clinic., (© 2023 British Pharmacological Society.)

Neuroaesthetic research has focused on neural predictive processes involved in the encounter with art stimuli or the related evaluative judgements, and it has been mainly conducted unimodally. Here, with electroencephalography, magnetoencephalography and an affective priming protocol, we investigated whether and how the neural responses to non-representational aesthetic stimuli are top-down modulated by affective representational (i.e. semantically meaningful) predictions between audition and vision. Also, the neural chronometry of affect processing of these aesthetic stimuli was investigated. We hypothesized that the early affective components of crossmodal aesthetic responses are dependent on the affective and representational predictions formed in another sensory modality resulting in differentiated brain responses, and that audition and vision indicate different processing latencies for affect. The target stimuli were aesthetic visual patterns and musical chords, and they were preceded by a prime from the opposing sensory modality. We found that early auditory-cortex responses to chords were more affected by valence than the corresponding visual-cortex ones. Furthermore, the assessments of visual targets were more facilitated by affective congruency of crossmodal primes than the acoustic targets. These results indicate, first, that the brain uses early affective information for predictively guiding aesthetic responses; second, that an affective transfer of information takes place crossmodally, mainly from audition to vision, impacting the aesthetic assessment. This article is part of the theme issue 'Art, aesthetics and predictive processing: theoretical and empirical perspectives'.

Approaching rewards and avoiding punishments is a fundamental aspect of behavior, yet individuals differ in the extent of these behavioral tendencies. One popular method to assess differences in approach-avoidance tendencies and even modify them, is using behavioral tasks in which spontaneous responses to differently valenced stimuli are assessed (e.g., the visual joystick and the manikin task). Understanding whether these reaction-time-based tasks map onto the same underlying constructs, how they predict interindividual differences in theoretically related constructs and how reliable they are, seems vital to make informed judgements about current findings and future studies. In this preregistered study, 168 participants (81 self-identified men, 87 women) completed emotional face versions of these tasks as well as an alternative, foraging-based paradigm, the approach-avoidance-conflict task, and answered self-report questionnaires regarding anxiety, aggression, depressive symptoms, behavioral inhibition and activation. Importantly, approach-avoidance outcome measures of the two reaction-time-based tasks were unrelated with each other, showed little relation to self-reported interindividual differences and had subpar internal consistencies. In contrast, the approach-avoidance-conflict task was related to behavioral inhibition and aggression, and had good internal consistencies. Our study highlights the need for more research into optimizing behavioral approach-avoidance measures when using task-based approach-avoidance measures to assess interindividual differences., (© 2023. The Author(s).)

Processing speed is a crucial ability that changes over the course of the lifespan. Training interventions on processing speed have shown promising effects and have been associated with improved cognitive functioning. While training-related changes in processing speed are often studied using reaction times (RTs) and error rates, these measures provide limited insight into the mechanisms underlying changes during training. The drift-diffusion model provides estimates of the cognitive processes underlying speeded decision tasks, such as the rate of evidence accumulation (drift rate), response strategies (boundary separation), as well as time for other processes such as stimulus encoding and motor response (non-decision time). In the current study, we analyzed existing data of an extensive multi-session training intervention (von Bastian & Oberauer, 2013) to disentangle changes in drift rate, boundary separation, and non-decision time during training of different speeded choice-RT tasks. During this training intervention, 30 participants performed 20 training sessions over the course of four weeks, completing three tasks each session: a face-matching, a pattern-matching, and a digit-matching task. Our results show that processing speed training increased drift rates throughout training. Boundary separation and non-decision time decreased mostly during the initial parts of training. This pattern of prolonged training-related changes in rate of evidence accumulation as well as early changes in response strategy and non-decision processes was observed across all three tasks. Future research should investigate how these training-related changes relate to improvements in cognitive functioning more broadly., Competing Interests: The authors have no competing interests to declare., (Copyright: © 2023 The Author(s).)

Rationale: Balancing approach of positive and avoidance of negative stimuli is essential when faced with approach-avoidance conflicts, e.g., situations with both positive and negative outcomes. This balance is disturbed in several mental disorders, e.g., excessive avoidance in anxiety disorders, and heightened approach in substance use disorders. Since stress is assumed to impact these disorders' etiology and maintenance, it seems crucial to understand how stress influences behavior in approach-avoidance conflicts. Indeed, some studies suggested altered approach-avoidance behavior under acute stress, but the mechanism underlying these effects is unknown., Objectives: Investigate how the pharmacological manipulation of major stress mediators (cortisol and noradrenaline) influences task-based approach-avoidance conflict behavior in healthy individuals., Methods: Ninety-six participants (48 women, 48 men) received either 20mg hydrocortisone, 20mg yohimbine, both, or placebo before performing a task targeting foraging under predation in a fully crossed double-blind between-subject design. Moreover, we investigated effects of gender and endogenous testosterone and estradiol levels on approach-avoidance behavior., Results: While biological stress markers (cortisol concentration, alpha amylase activity) indicated successful pharmacological manipulation, behavior in approach-avoidance conflicts was not affected as expected. Although yohimbine administration affected risky foraging latency under predation, we found no main effect of hydrocortisone or their interaction on behavior. In contrast, we found gender differences for almost all behavioral outcome measures, which might be explained by differences in endogenous testosterone levels., Conclusions: The investigated major stress mediators were not sufficient to imitate previously shown stress effects on approach-avoidance conflict behavior. We discuss potential reasons for our findings and implications for future research., (© 2023. The Author(s).)

Research investigating the effects of carbohydrate (CHO) mouth rinsing on neurocognitive functions is currently limited and has yielded inconsistent results. In this study, we employed the event-related potential (ERP) electroencephalography technique to investigate the effect of CHO mouth rinsing on electrophysiological correlates of visuospatial attention. Using a double-blind, non-nutritive sweetener (NNS)-controlled, within-subjects design, 53 young adults performed a standard cognitive task (modified Simon task) on two separate days in a fasted state (16 h). Intermittently, mouth rinsing was performed either with a CHO (glucose, 18%, 30 mL) or an NNS solution (aspartame, 0.05%, 30 mL). Results revealed that relative to NNS, electrophysiological correlates of both more bottom-up controlled visuospatial attention (N1 pc -ERP component) were decreased in response to CHO rinsing. In contrast, compared to NNS, more top-down controlled visuospatial attention (N2 pc -ERP component) was increased after CHO rinsing. Behavioral performance, however, was not affected by mouth rinsing. Our findings suggest that orosensory signals can impact neurocognitive processes of visuospatial attention in a fasted state. This may suggest a central mechanism underlying the ergogenic effects of carbohydrate mouth rinsing on endurance performance could involve modulations of attentional factors. Methodologically, our study underlines that understanding the effects of carbohydrate mouth rinsing at the central level may require combining neuroscientific methods and manipulations of nutritional states.

The haptic exploration and aesthetic processing of all kinds of materials' surfaces are part of everyday life. In the present study, functional near-infrared spectroscopy (fNIRS) was used to investigate the brain correlates of active fingertip exploration of material surfaces and subsequent aesthetic judgments of their pleasantness (feels good or bad?). In absence of other sensory modalities, individuals (n = 21) performed lateral movements on a total of 48 textile and wood surfaces varying in terms of their roughness. Behavioral results confirmed the influence of the stimuli's roughness on aesthetic judgments, with smoother textures being rated as feeling better than rough textures. At the neural level, fNIRS activation results revealed an overall increased engagement of the contralateral sensorimotor areas as well as left prefrontal areas. Moreover, the perceived pleasantness modulated specific activations of left prefrontal areas with increasing pleasantness showing greater activations of these regions. Interestingly, this positive relationship between the individual aesthetic judgments and brain activity was most pronounced for smooth woods. These results demonstrate that positively valenced touch by actively exploring material surfaces is linked to left prefrontal activity and extend previous findings of affective touch underlying passive movements on hairy skin. We suggest that fNIRS can be a valuable tool to provide new insights in the field of experimental aesthetics., Competing Interests: Declaration of competing interest The authors declare that there is no conflict of interests., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Cartilage repair has been studied extensively in the context of injury and disease, but the joint's management of regular sub-injurious damage to cartilage, or 'wear and tear,' which occurs due to normal activity, is poorly understood. We hypothesize that this cartilage maintenance is mediated in part by cells derived from the synovium that migrate to the worn articular surface. Here, we demonstrate in vitro that the early steps required for such a process can occur. First, we show that under physiologic mechanical loads, chondrocyte death occurs in the cartilage superficial zone along with changes to the cartilage surface topography. Second, we show that synoviocytes are released from the synovial lining under physiologic loads and attach to worn cartilage. Third, we show that synoviocytes parachuted onto a simulated or native cartilage surface will modify their behavior. Specifically, we show that synoviocyte interactions with chondrocytes lead to changes in synoviocyte mechanosensitivity, and we demonstrate that cartilage-attached synoviocytes can express COL2A1, a hallmark of the chondrogenic phenotype. Our findings suggest that synoviocyte-mediated repair of cartilage 'wear and tear' as a component of joint homeostasis is feasible and is deserving of future study., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

There is currently insufficient knowledge of gestational age dependent medicine disposition in neonates. Accordingly, the use of off-label medication, i.e., use of medicines outside its approved marketing authorization, is high in the neonatal departments. By using data from the Danish National Pharmaceutical Hospital Purchase Database, we identified the most commonly occurring medications and calculated the on/off-label ratios for premature and term neonates. Data was extracted on ATC level 5 and based on defined daily doses as per WHO. Data covered the 4 high-level NICUs and 10 of 13 of the intermediate/standard level Danish neonatal departments. Of the identified medication, 87% and 70% did not have approved marketing authorization for use in premature and full-term neonates, respectively. Furthermore, one-fifth of the top 100 medicines did not have a (Danish) marketing license. Overall, off-label medication was widespread covering virtually all ATC groups and no ATC group had an off-label level lower than 50% (range 50%-100%). Finally, in 21% of medications, additives from 8 different chemical groups with potential deleterious effects for neonates were identified. In conclusion, off-label medication in the Danish neonatal departments is widespread. The pharmaceutical industry is unlikely to solve this problem, and we may for a very long time be occasionally forced to use off-label medication. Practical solution must therefore come from multidisciplinary clinical and academic collaboration. Use of formulation list as guidance for prescriptions and NICU-friendly galenic formulations may mitigate the problem temporarily while waiting for definitive studies., (© 2022 The Authors. Pharmacology Research & Perspectives published by British Pharmacological Society and American Society for Pharmacology and Experimental Therapeutics and John Wiley & Sons Ltd.)

Goal-directed behavior is assumed to require processes of attentional biasing to counter unwanted action tendencies elicited by distracting stimulus information. This is particularly so if stimulus categories that define the target and the distractor frequently reverse, requiring participants to respond to previously ignored stimulus categories and vice versa. In the current study, we investigated control strategies under such conditions. Specifically, we assessed trial-to-trial modulation of distractor-interference (i.e., congruency sequence effect, CSE) in a temporal flanker task associated with repetition versus alternation of the assignment of stimulus category (i.e., digits, letters) to targets and distractors (i.e., the character presented second or first, respectively) under conditions of a long SOA of 1000 ms (Experiment 1A) and 1200 ms (Experiment 1B). Whereas previous research, using a shorter SOA, suggested temporal-order control (i.e., the occurrence of a CSE in both repetition and-albeit less pronounced-alternation trials), lengthening the distractor-target SOA resulted in a CSE confined to repetition trials, suggesting strong or exclusive reliance on stimulus categories for attentional control (Experiment 1A and B). Adding a redundant stimulus feature (i.e., color), discriminating targets and distractors, eliminated the difference of CSE patterns in repetition and alternation trials (Experiment 2). Together, our results suggest that the strength of concurrently applied control strategies or the choice of a particular control strategy depend on contextual factors., (© 2022. The Author(s).)

The list-level proportion congruency effect (PCE) and the context-specific PC (CSPC) effect are typical findings in experimental conflict protocols, which competing explanations attribute to different mechanisms. Of these mechanisms, stimulus-unspecific conflict-induced selectivity adjustments have attracted the most interest, from various disciplines. Recent methodological advances have yielded an experimental procedure for entirely ruling out all stimulus-specific alternatives. However, there is a stimulus-unspecific alternative-temporal learning-which cannot even be ruled out as the sole cause of either effect with any established experimental procedure. That is because it is very difficult to create a scenario in which selectivity adjustments and temporal learning make different predictions-with traditional approaches, it is arguably impossible. Here, we take a step towards solving this problem, and experimentally dissociating the two mechanisms. First, we present our novel approach which is a combination of abstract experimental conditions and theoretical assumptions. As we illustrate with two computational models, given this particular combination, the two mechanisms predict opposite modulations of an as yet unexplored hybrid form of the list-level PCE and the CSPC effect, which we term context-dependent PCE (CDPCE). With experimental designs that implement the abstract conditions properly, it is therefore possible to rule out temporal learning as the sole cause of stimulus-unspecific adaptations to PC, and to unequivocally attribute the latter, at least partially, to selectivity adjustments. Secondly, we evaluate methodological and theoretical aspects of the presented approach. Finally, we report two experiments, that illustrate both the promise of and a potential challenge to this approach., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2022 Sprengel et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Human research has shown interactions between rewards and cognitive control. In animal models of affective neuroscience, reward administration typically involves administering orosensory sugar signals (OSS) during caloric-deprived states. We adopted this procedure to investigate neurophysiological mechanisms of reward-cognitive control interactions in humans. We predicted that OSS would affect neurophysiological and behavioral indices of error processing oppositely, depending on the relative weight of the OSS-induced 'wanting' and 'liking' components of reward. We, therefore, conducted a double-blind, non-nutritive sweetener-controlled study with a within-subject design. Fasted (16 hr) participants ( N  = 61) performed a modified Flanker task to assess neurophysiological (error-related negativity [N e /ERN]) and behavioral (post-error adaptations) measures of error processing. Non-contingent to task performance, we repeatedly administered either a sugar (glucose) or non-nutritive sweetener (aspartame) solution, which had to be expulsed after short oral stimulation to prevent post-oral effects. Consistent with our hypothesis on how 'liking' would affect N e /ERN amplitude, we found the latter to be decreased for sugar compared to aspartame. Unexpectedly, we found post-error accuracy, instead of post-error slowing, to be reduced by sugar relative to aspartame. Our findings suggest that OSS may interact with error processing through the 'liking' component of rewards. Adopting our reward-induction procedure (i.e. administering OSS in a state of high reward sensitivity [i.e. fasting], non-contingent to task performance) might help future research investigating the neural underpinnings of reward-cognitive control interactions in humans.

Natural occurrences and the choice of specific materials have a major impact on the experience of the physical environment. The results of a recent study using a free listing task involving only adjectives suggested that the conceptual structure of the aesthetics of materials is structured by sensorial, neutrally valenced, descriptive terms, while showing no primacy of beauty. The present article examined the conceptual structure underlying the aesthetic experience of various materials using a different methodological approach. Applying a technique based upon semantic differentials, individuals in the present study (n = 272) were asked to judge the applicability of the most frequently listed terms in the previous study to the aesthetics of different materials. Overall, the results of multiple analyses yielded a converging picture for the two studies. Additionally, as materials constitute the basis of complete entities, the role of products in the conceptual representation of the aesthetics of materials was investigated with an exploratory approach. No support was found for the hypothesis that products play such a role. Finally, limitations regarding the outcome of the present study are discussed. All things considered, the results of this study highlight the uniqueness of the aesthetics of materials and its distinctness from the conceptual representations underlying most other aesthetic domains., Competing Interests: The authors have declared that no competing interests exist.

Response conflict evoked by a distractor stimulus object in interference tasks is associated with longer reaction times and a pronounced ERP component referred to as the fronto-central N2. Increasing the proportion congruency (PC, i.e., the proportion of trials in which target and distractor are associated with the same response) is assumed to enhance distractor-evoked conflict via defocusing of attention. Findings concerning the effect of the PC on the N2 in Eriksen flanker task experiments have been inconsistent, however, lending little support to the notion that the N2 reflects a conflict monitoring process. Here, we analyze the N2 in a temporal flanker task, in which the distractor stimuli, presented in advance of the target, elicit pronounced activation of the associated responses (as inferred from the lateralized readiness potential) when the PC is high. Consistent with result pattern obtained in other tasks involving successive presentation of the distractor and the target, conflict trials in a high-PC condition evoked a particularly large N2. These findings accord with the assumption that the N2 reflects either conflict monitoring or conflict-induced regulatory measures (i.e., reactive control). In light of the discrepancy of results obtained under conditions of simultaneous and successive distractor-target presentation, we speculate that the N2 is pronounced in situations that offer strong hints for classifying dominating response activation as incorrect, possibly reflecting particular control to counter this activation. Additional properties of the temporal flanker task, related to ERP investigations, are discussed., (© 2022 The Authors. Psychophysiology published by Wiley Periodicals LLC on behalf of Society for Psychophysiological Research.)

Being moved has received increased attention in emotion psychology as a social emotion that fosters bonds between individuals and within communities. This increased attention, however, has also sparked debates about whether the term "being moved" refers to a single distinct profile of emotion components or rather to a range of different emotion profiles. We addressed this question by investigating lay conceptions of the emotion components (i.e., elicitors, cognitive appraisals, subjective feelings, bodily symptoms, and consequences for thought/action) of "bewegt sein" (the German term for "being moved"). Participants (N = 106) provided written descriptions of both a moving personal experience and their conceptual prototype of "being moved," which were subjected to content analysis to obtain quantitative data for statistical analyses. Based on latent class analyses, we identified two classes for both the personal experiences (joyfully-moved and sadly-moved classes) and the being-moved prototype (basic-description and extended-description classes). Being joyfully moved occurred when social values and positive relationship experiences were salient. Being sadly moved was elicited by predominantly negative relationship experiences and negatively salient social values. For both classes, the most frequently reported consequences for thought/action were continued cognitive engagement, finding meaning, and increased valuation of and striving for connectedness/prosociality. Basic descriptions of the prototype included "being moved" by positive or negative events as instances of the same emotion, with participants in the extended-description class also reporting joy and sadness as associated emotions. Based on our findings and additional theoretical considerations, we propose that the term "being moved" designates an emotion with an overall positive valence that typically includes blends of positively and negatively valenced emotion components, in which especially the weight of the negative components varies. The emotion's unifying core is that it involves feeling the importance of individuals, social entities, and abstract social values as sources of meaning in one's life., Competing Interests: The authors have declared that no competing interests exist.

The unique contribution of the serotonin transporter-linked polymorphic region (5-HTTLPR), intronic region 2 (STin2), and monoamine oxidase A (MAO-A) genes to individual differences in personality traits has been widely explored, and research has shown that certain forms of these polymorphisms relate to impulsivity and impulsivity-related disorders. Humans showing these traits are also described as having an asymmetrical prefrontal cortical activity when compared to others. In this explorative study, we examine the relationship between serotonergic neurotransmission polymorphisms, cortical activity features (prefrontal alpha asymmetry, individual alpha peak frequency [iAPF]), emotion-related and non-emotion-related impulsivity in humans. 5-HTTLPR, MAO-A, and STin2 polymorphisms were assessed in blood taken from 91 participants with high emotion-related impulsivity levels. Sixty-seven participants completed resting electroencephalography and a more comprehensive impulsivity index. In univariate analyses, iAPF correlated with both forms of emotion-related impulsivity. In multiple linear regression models, 5-HTTLPR polymorphism (model 1, adj. R 2  = 15.2%) and iAPF were significant interacting predictors of emotion-related impulsivity, explaining a large share of the results' variance (model 2, adj. R 2  = 21.2%). Carriers of the low transcriptional activity 5-HTTPLR and MAO-A phenotypes obtained higher emotion-related impulsivity scores than others did. No significant results were detected for non-emotion-related impulsivity or for a form of emotion-related impulsivity involving cognitive/motivational reactivity to emotion. Our findings support an endophenotypic approach to impulsivity, showing that tri-allelic 5-HTTLPR polymorphism, iAPF, and their interaction are relevant predictors of one form of emotion-related impulsivity., (© 2022. The Author(s).)

The ability to interact and adapt to the surrounding environment is vital for bacteria that colonise various niches and organisms. One strategy developed by Gram-negative bacteria is to secrete exoprotein substrates via the type II secretion system (T2SS). The T2SS is a proteinaceous complex spanning the bacterial envelope that translocates folded proteins such as toxins and enzymes from the periplasm to the extracellular milieu. In the T2SS, a cytoplasmic ATPase elongates in the periplasm the pseudopilus, a non-covalent polymer composed of protein subunits named pseudopilins, and anchored in the inner membrane by a transmembrane helix. The pseudopilus polymerisation is coupled to the secretion of substrates. The T2SS of Dickeya dadantii secretes more than 15 substrates, essentially plant cell wall degrading enzymes. In D. dadantii, the major pseudopilin or the major subunit of the pseudopilus is called OutG. To better understand the mechanism of secretion of these numerous substrates via the pseudopilus, we have been studying the structure of OutG by NMR. Here, as the first part of this study, we report the 1 H, 15 N and 13 C backbone and sidechain chemical shift assignment of the periplasmic domain of OutG and its NMR derived secondary structure., (© 2022. The Author(s).)

Liking and pleasantness are common concepts in psychological emotion theories and in everyday language related to emotions. Despite obvious similarities between the terms, several empirical and theoretical notions support the idea that pleasantness and liking are cognitively different phenomena, becoming most evident in the context of emotion regulation and art enjoyment. In this study it was investigated whether liking and pleasantness indicate behaviourally measurable differences, not only in the long timespan of emotion regulation, but already within the initial affective responses to visual and auditory stimuli. A cross-modal affective priming protocol was used to assess whether there is a behavioural difference in the response time when providing an affective rating to a liking or pleasantness task. It was hypothesized that the pleasantness task would be faster as it is known to rely on rapid feature detection. Furthermore, an affective priming effect was expected to take place across the sensory modalities and the presentative and non-presentative stimuli. A linear mixed effect analysis indicated a significant priming effect as well as an interaction effect between the auditory and visual sensory modalities and the affective rating tasks of liking and pleasantness: While liking was rated fastest across modalities, it was significantly faster in vision compared to audition. No significant modality dependent differences between the pleasantness ratings were detected. The results demonstrate that liking and pleasantness rating scales refer to separate processes already within the short time scale of one to two seconds. Furthermore, the affective priming effect indicates that an affective information transfer takes place across modalities and the types of stimuli applied. Unlike hypothesized, liking rating took place faster across the modalities. This is interpreted to support emotion theoretical notions where liking and disliking are crucial properties of emotion perception and homeostatic self-referential information, possibly overriding pleasantness-related feature analysis. Conclusively, the findings provide empirical evidence for a conceptual delineation of common affective processes., Competing Interests: Enter: The authors have declared that no competing interests exist.

Neonatal brachial plexus palsy (NBPP) occurs in approximately 1.5 of every 1,000 live births. The majority of children with NBPP recover function of the shoulder. However, the long-term risk of osteoarthritis (OA) in this population is unknown. The purpose of this study was to investigate the development of OA in a mouse model of transient neonatal shoulder paralysis. Neonatal mice were injected twice per week for 4 weeks with saline in the right supraspinatus muscle (Saline, control) and botulinum toxin A (BtxA, transient paralysis) in the left supraspinatus muscle, and then allowed to recover for 20 or 36 weeks. Control mice received no injections, and all mice were sacrificed at 24 or 40 weeks. BtxA mice exhibited abnormalities in gait compared to controls through 10 weeks of age, but these differences did not persist into adulthood. BtxA shoulders had decreased bone volume (-9%) and abnormal trabecular microstructure compared to controls. Histomorphometry analysis demonstrated that BtxA shoulders had higher murine shoulder arthritis scale scores (+30%), and therefore more shoulder OA compared to controls. Articular cartilage of BtxA shoulders demonstrated stiffening of the tissue. Compared with controls, articular cartilage from BtxA shoulders had 2-fold and 10-fold decreases in Dkk1 and BMP2 expression, respectively, and 3-fold and 14-fold increases in Col10A1 and BGLAP expression, respectively, consistent with established models of OA. In summary, a brief period of paralysis of the neonatal mouse shoulder was sufficient to generate early signs of OA in adult cartilage and bone., (© 2021 Orthopaedic Research Society. Published by Wiley Periodicals LLC.)

Do we savour aesthetic experiences less when distracted by interrupted tasks, work rumination, or stress? Evidence suggests that the ability to concentrate on the aesthetic experience is crucial for initiating a processing mode of conscious aesthetic reception that results in more positive emotions. When working memory resources are otherwise occupied, people are less able to concentrate on aesthetic experiences. Aesthetic savouring, in particular-a cognitive form of emotion regulation that is used to maintain and extend aesthetic experiences-is thought to be impaired under those circumstances. We conducted three investigations to examine how conditions that are known to deplete working memory resources affect the savouring of aesthetic experiences. In Study 1, participants rated beauty and savouring felt from encounters with visual stimuli in a controlled laboratory setting after an interruption of a writing task. Aesthetic experience was hampered if participants were interrupted. In two field investigations, we demonstrated that work-related rumination (Study 2, N = 329) and stress (Study 3, N = 368) are inversely related to the savouring felt from opera, theatre, or cabaret pieces. These findings highlight the importance of concentrating on aesthetic experiences so that the perceiver can fully benefit from them. We also discuss implications for cognitive models of working memory and for health and well-being.

Background . Modern working conditions require an increased amount of spatial mobility from employed personnel. Research suggests that different types of job mobility might exert negative effects on well-being and health, and additionally have different costs and benefits for the work and the social domains. Methods . Using data from 3191 members of the German Armed Forces, we investigated the effects of four different types of job mobility (long-distance commuters, overnighters, residential mobiles and multi-mobiles) in contrast to non-mobiles on employees' subjective job performance as an occupationally relevant outcome. Moreover, we expected beliefs about social and occupational advantages and disadvantages to mediate the effects of job mobility on subjective job performance. Results . A single concrete event during relocation had fewer negative consequences compared to the effects of circular mobility or multi-mobility. Moreover, beliefs about occupational and social advantages and disadvantages were differently associated with the different types of job mobility and partially mediated the direct effects of job mobility on job performance. Conclusions . Not all types of job mobility are an impairment and extra-organizational stress. Rather, the evaluation and perception of occupational and social (dis)advantages is crucial for the effects of different types of job mobility on organizational relevant outcomes.

Background: Aesthetic experiences elicit a wide range of positive emotions and have a positive impact on various health outcomes. In this context, savoring refers to a cognitive form of emotion regulation used to maintain and extend positive emotional experiences and is considered to contribute to health and well-being. Chronic pain has been linked to reduced reward-seeking behavior. This is the first study to investigate the relationship between self-reported chronic pain and savoring., Methods: We conducted an anonymous cross-sectional survey in a large non-clinical sample (opera, theater, and cabaret visitors; n = 322). The variables were assessed with a two-item-questionnaire., Results: Self-reported chronic pain was significantly negatively correlated with savoring (r = -.547)., Conclusion: Altogether, this result helps to develop a better understanding of the effects of chronic pain in humans and to shed light on state-dependent differences in aesthetic experiences., Competing Interests: The authors have declared that no competing interests exist.

While body modifications have increasingly gained acceptance and popularity, how different subpopulations aesthetically appreciate tattoos remains unclear. The present study aimed to investigate the conceptual structure underlying tattoo aesthetics, focusing on the effects of internalized social norms and expertise. Using a timed free-listing task, three groups (≤49 years, ≥50 years, and experts) comprising 497 participants were asked to write down adjectives that could describe tattoo aesthetics. Statistical analyses of frequency, cognitive salience indices, co-occurrence dimensions, semantic dimensions, similarity measures, and valences were applied and, to directly compare the three groups, a generalized Procrustes analysis was applied. The variance and complexity with which individuals verbally expressed their perceived aesthetic appeal of tattoos were highlighted. However, the results do not reveal a unified concept of beauty, nor do they present a clear bipolar dimension of beautiful/ugly for two of the three groups. Nevertheless, the concept of beauty was found to be prominent in tattoo aesthetics, and aesthetic and descriptive-evaluative dimensions were identified, with terms such as beautiful, ugly, multicolored, and interesting being the most notable adjectives, although not with the highest valence. Possible factors explaining the intracultural differences between the three groups are also discussed., (Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.)

The congruency sequence effect (CSE) describes the performance difference of congruent trials (in which target and distractor stimuli are associated with the same response) compared to incongruent trials (in which target and distractor stimuli are associated with different responses) as a function of the preceding congruency level (congruent trials relative to incongruent trials). The CSE is commonly interpreted as a measure of conflict-induced attentional adjustment. Although previous research has made substantial progress aiming at controlling for alternative explanations of the CSE, both task-specific and fundamental confounds have remained. In the current study, we used a temporal flanker task, in which two stimuli (i.e., distractor and target) are presented in rapid succession, and extended previous demonstrations of a CSE in flanker tasks by deconfounding target-distractor congruency from perceptual similarity. Using a four-choice task, we could also control for the reversal of distractor-response priming after incongruent trials (which is only feasible in two-choice tasks). Furthermore, we controlled for all confounds based on the sequence (i.e., repetition versus alternation) of the congruency level - such as feature sequence effects, distractor-response contingency switch costs, or temporal learning - by probing the allocation of attention to the points in time of presentation of the first and the second stimulus of a trial. This was achieved by intermixing trials of a temporal search task. The performance accuracy results in this task were consistent with a stronger attentional bias in favor of the target stimulus' temporal position after incongruent than after congruent trials., (© 2021. The Author(s).)

Although statistical regularities in the environment often go explicitly unnoticed, traces of implicit learning are evident in our neural activity. Recent perspectives have offered evidence that both pre-stimulus oscillations and peri-stimulus event-related potentials are reliable biomarkers of implicit expectations arising from statistical learning. What remains ambiguous, however, is the origination and development of these implicit expectations. To address this lack of knowledge and determine the temporal constraints of expectation formation, pre-stimulus increases in alpha/beta power were investigated alongside a reduction in the N170 and a suppression in peri-/post-stimulus gamma power. Electroencephalography was acquired from naive participants who engaged in a gender classification task. Participants were uninformed, that eight face images were sorted into four reoccurring pairs which were pseudorandomly hidden amongst randomly occurring face images. We found a reduced N170 for statistically expected images at left parietal and temporo-parietal electrodes. Furthermore, enhanced gamma power following the presentation of random images emphasized the bottom-up processing of these arbitrary occurrences. In contrast, enhanced alpha/beta power was evident pre-stimulus for expected relative to random faces. A particularly interesting finding was the early onset of alpha/beta power enhancement which peaked immediately after the depiction of the predictive face. Hence, our findings propose an approximate timeframe throughout which consistent traces of enhanced alpha/beta power illustrate the early prioritisation of top-down processes to facilitate the development of implicitly cued face-related expectations., Competing Interests: The authors have declared that no competing interests exist.

Infectious diseases are an existential health threat, potentiated by emerging and re-emerging viruses and increasing bacterial antibiotic resistance. Targeted treatment of infectious diseases requires precision diagnostics, especially in cases where broad-range therapeutics such as antibiotics fail. There is thus an increasing need for new approaches to develop sensitive and specific in vitro diagnostic (IVD) tests. Basic science and translational research are needed to identify key microbial molecules as diagnostic targets, to identify relevant host counterparts, and to use this knowledge in developing or improving IVD. In this regard, an overlooked feature is the capacity of pathogens to adhere specifically to host cells and tissues. The molecular entities relevant for pathogen-surface interaction are the so-called adhesins. Adhesins vary from protein compounds to (poly-)saccharides or lipid structures that interact with eukaryotic host cell matrix molecules and receptors. Such interactions co-define the specificity and sensitivity of a diagnostic test. Currently, adhesin-receptor binding is typically used in the pre-analytical phase of IVD tests, focusing on pathogen enrichment. Further exploration of adhesin-ligand interaction, supported by present high-throughput "omics" technologies, might stimulate a new generation of broadly applicable pathogen detection and characterization tools. This review describes recent results of novel structure-defining technologies allowing for detailed molecular analysis of adhesins, their receptors and complexes. Since the host ligands evolve slowly, the corresponding adhesin interaction is under selective pressure to maintain a constant receptor binding domain. IVD should exploit such conserved binding sites and, in particular, use the human ligand to enrich the pathogen. We provide an inventory of methods based on adhesion factors and pathogen attachment mechanisms, which can also be of relevance to currently emerging pathogens, including SARS-CoV-2, the causative agent of COVID-19.

The effects of lexical meaning and lexical familiarity on auditory deviance detection were investigated by presenting oddball sequences of words, while participants ignored the stimuli. Stimulus sequences were composed of words that were varied in word class (nouns vs. functions words) and frequency of language use (high vs. low frequency) in a factorial design with the roles of frequently presented stimuli (Standards) and infrequently presented ones (Deviants) were fully crossed. Deviants elicited the Mismatch Negativity component of the event-related brain potential. Modulating effects of lexical meaning were obtained, revealing processing advantages for denotationally meaningful items. However, no effect of word frequency was observed. These results demonstrate that an apparently low-level function, such as auditory deviance detection utilizes information from the mental lexicon even for task-irrelevant stimuli., (Copyright © 2021 Elsevier Inc. All rights reserved.)

Background and Purpose: The outbreak of the COVID-19 pandemic has resulted in an overall decline in fractures. However, the amount of hip fractures has remained relatively stable throughout the period. The objective of this study is to investigate the impact of perioperative COVID-19 infections on mortality among hip fracture patients., Methods: A meta-analysis was performed by collecting current data available through a systematic literature search in the PubMed database. The search was performed Oct 18 2020., Results: The meta-analysis was conducted on a trial population consisting of 1.272 hip fracture patients with a pooled prevalence of COVID-19 of 18%. Mortality among hip fracture patients without a perioperative COVID-19 infection was 7.49%. Mortality among hip fracture patients infected with COVID-19 perioperatively was associated with an odds ratio of 6.70 [(95% CI 4.64-9.68), p < 0.00001, I 2  = 41%]. A sensitivity analysis showed no major impact of assumptions regarding varying definitions of COVID-19 statuses among the included studies., Conclusion: Perioperative infections with COVID-19 in hip fracture patients are correlated with a significantly increased mortality. The meta-analysis showed a pooled odds ratio of 6.70 [(95% CI 4.64-9.68), p < 0.00001, I 2  = 41%].

Aesthetic experiences have been distinguished from other experiences based on an aesthetic mode of processing that often entails concentrating working memory resources on the aesthetic stimulus. Since working memory is a limited-capacity system, there should be a trade-off between available resources and the aesthetic experience. To test whether the intensity of the aesthetic experience is reduced if working memory resources are otherwise occupied, we employed an experience sampling method. One hundred and fifteen undergraduate students (45% female; Mage = 23.50 years, SD = 2.72 years) participated in a 2-week experience sampling study and furnished a total of 15,047 reports of their aesthetic experiences. As measures of current working memory resources, participants answered questions regarding their current working memory load and whether they were engaged in a second task. In addition, they reported whether they had had an aesthetic experience and how much they had savored the aesthetic experience. Multilevel modeling was used for data analysis. A higher working memory load was associated with fewer aesthetic experiences and reduced the savoring of aesthetic experiences. Second tasks, however, that were perceived as demanding and requiring a lot of concentration enhanced the savoring of aesthetic experiences. In sum, other goal-oriented behavior that requires working memory resources appears to conflict with aesthetic experiences in everyday life., Competing Interests: The authors have declared that no competing interests exist.

Do individuals modify their bodies in order to be unique? The present study sought to investigate need for uniqueness (NfU) subcomponents as possible motives for modifying one's body. To this end, the study obtained information from 312 participants about their NfU (using the German NfU-G global scale and three sub-scales) and their body modifications (tattoos, piercings, and extreme body modifications such as tongue splitting). By analyzing the three subcomponents of NfU, the study was able to investigate the differential relationship of the sub-scales with the outcome measures, which facilitated a fine-grained understanding of the NfU-body-modification relationship. The study found that tattooed, pierced, and extreme-body-modified individuals had higher NfU-G scores than individuals without body modifications. Moreover, it seemed that individuals with tattoos took a social component into consideration while lacking concern regarding others' reaction toward their tattoos, although not wanting to cause affront. Pierced and extreme-body-modified individuals, contrarily, tended to display a propensity to actively flout rules and not worry about others' opinions on their modifications. However, although statistically significant, the effect size (d) for the NfU-G differences in the tattooed and pierced participants' mean scores was small to medium in all three subcomponents. The extreme-body-modified group presented medium and medium to large effects. Further, the study observed that the number of body modifications increased with an increasing NfU in tattooed and pierced individuals. These findings demonstrated multifaceted interrelations between the NfU, its subcomponents, and the three kinds of body modifications investigated in the present study., Competing Interests: The authors have declared that no competing interests exist.

Tactical breathing (TB) is used by military and law enforcement personnel to reduce stress and maintain psychomotor and cognitive performance in dangerous situations (Grossman and Christensen, in On combat: the psychology and physiology of deadly conflict in war and in peace, PPCT Research Publications, Belleville, 2008). So far, empirical evidence on the effectiveness of TB is limited and there are breathing techniques that are easier to learn and to apply. This study compared the effectiveness of tactical breathing and prolonged exhalation (ProlEx) under laboratory conditions. Thirty healthy participants performed a Stroop interference task under time pressure and noise distraction. Time pressure was induced with short inter-trial intervals of 350 ms and short trial durations of 1500 ms. Acoustic distraction was realised with white noise with intensity increasing from 77 to 89 dB SPL over the course of an experimental block. In a counterbalanced repeated-measures design, participants used either TB or ProlEx to reduce the induced psychological and physiological arousal. Stress reactions were assessed on the subjective level (Steyer et al., in Multidimensional mood questionnaire (MDMQ), Hogrefe, Göttingen, 1997) and on the physiological level (heart rate, heart rate variability, electrodermal activity). Results showed no significant differences between breathing techniques on the subjective level. While participants showed a lower physiological arousal in the TB condition, better performance was achieved in the ProlEx condition. Results indicate that TB may be superior in passive coping conditions, while ProlEx is more effective when active coping is required.

Our theoretical model (Menninghaus et al., 2019) defines aesthetic emotions by reference to their role in aesthetic evaluation, and specifically as being predictive of aesthetic liking/disliking. Skov and Nadal (2020) dismiss the construct of "aesthetic emotions" as a "dated supposition" adopted from a "speculative" tradition and assert that there are no such emotions. Accordingly, they question all pieces of empirical evidence we referred to as supporting our model. In our response, we rebut these objections point by point and defend as well as expand the empirical evidence in support of our model. (PsycInfo Database Record (c) 2020 APA, all rights reserved).

CRISPR-Cas systems comprise diverse adaptive immune systems in prokaryotes whose RNA-directed nucleases have been co-opted for various technologies. Recent efforts have focused on expanding the number of known CRISPR-Cas subtypes to identify nucleases with novel properties. However, the functional diversity of nucleases within each subtype remains poorly explored. Here, we used cell-free transcription-translation systems and human cells to characterize six Cas12a single-effector nucleases from the V-A subtype, including nucleases sharing high sequence identity. While these nucleases readily utilized each other's guide RNAs, they exhibited distinct PAM profiles and apparent targeting activities that did not track based on phylogeny. In particular, two Cas12a nucleases encoded by Prevotella ihumii (PiCas12a) and Prevotella disiens (PdCas12a) shared over 95% amino-acid identity yet recognized distinct PAM profiles, with PiCas12a but not PdCas12a accommodating multiple G's in PAM positions -2 through -4 and T in position -1. Mutational analyses transitioning PiCas12a to PdCas12a resulted in PAM profiles distinct from either nuclease, allowing more flexible editing in human cells. Cas12a nucleases therefore can exhibit widely varying properties between otherwise related orthologs, suggesting selective pressure to diversify PAM recognition and supporting expansion of the CRISPR toolbox through ortholog mining and PAM engineering., (© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.)