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Water scarcity and disasters, particularly floods and droughts, are the most devastating disasters in Sri Lanka, causing severe damage to agriculture, lives and property. This condition has been worsen further during the Covid19, due to isolation and travel restrictions. Collaboration and effective watershed management among the stakeholders is one of the key solutions for effective operation, but watershed management is more complex and complicated due to its physical processes, and conflicts among stakeholders. This study attends to attract stakeholders and develop Decision Making System by integrating complex hydrological model with dam operation and diversion, based on topography, rainfall, evaporation with bias-corrected satellite data. Finally, we developed an Integrated System for decision makers for effective water resource management in River basins. The proposed approach was applied to the complex Mahaweli watershed in Sri Lanka, to address prevailing issues.

In this article, the catchment of Grand River North West (GRNW) in Mauritius was selected as a case study to develop an Integrated Flood Management Plan to address the flood risks that Mauritius is and will be facing due to climate change. The study contains three main components: (i) a climatology analysis for the past (2003–2018) and future climate (2025–2040) to assess the trends in floods under climate change, (ii) the development of a hydrological model to study the hydrological responses of the basin for extreme flooding events, and (iii) a GIS-based flood risk model to develop a risk map. The results show that extreme rainfall events exceeding 50 mm d−1 have a high likelihood of increasing. A flood vulnerability map for the basin was developed based on nine indicators to aid decision making at the village council area (VCA) level and guide financial investments flood mitigation measures. The study established a novel end-to-end approach to develop an Integrated Flood Management Plan based on a case study for a river basin in Mauritius.

The African regions have been affected by frequent flood-related disasters. To support decision-makers in flood disaster management, a real-time flood monitoring system was established in the Niger and Volta basins under the Water disaster platform to enhance climate resilience in Africa (WADiRE-Africa). To bridge a gap between data unavailability and inaccessibility, the near-real-time satellite rainfall data (GSMaP-NRT) was evaluated and found to have a very low correlation () with gauge data at daily scales and a higher correlation (>0.7) at monthly scales. The statistically bias-corrected GSMaP-NRT data was used in the Water and Energy Budget-based Rainfall-Runoff-Inundation (WEB-RRI) model to generate hydrological responses of the basins. As the simulated river discharges were overestimated, an additional correction method was employed for the GSMaP-NRT data to match well with observed discharges. The prototype system provides real-time flood information in both basins.

In light of the frequent occurrence of unprecedented heavy rains and floods attributable to climate change effects, the importance of the “River Basin Disaster Resilience and Sustainability by All” initiative is being emphasized and promoted strongly in Japan. For promoting discussions on a wide range of countermeasures for flood damage reduction by the initiative, this paper presents a trial estimation result of quantitative flood damage reduction effect by spillway installation on a riverine levee in a protected area where severe flood disasters have occurred in recent years. The result shows that the countermeasure considerably reduce the flooded area, the days required for drainage, and house flood depths. Results also indicate that the average annual damage to paddy fields in the area can be expected to decrease, although the inundation frequency will increase.

Extreme rainfall or heavy rainfall events (HREs) causes significant socio-economic damages annually affecting local development especially in developing countries. Thus, assessing changes in frequency and magnitude of HREs under climate change using global climate model (GCM) projections became ubiquitous to hydrological impact studies. Here, we present a framework for evaluating GCM's ability in reproducing the seasonal frequency of HREs in Davao River basin and the associated weather patterns that led to HREs. Our results show that HREs in MRI-AGCM 3.2S occurred 81 % in DJF and 4 % in JJA, which showed over(under) estimation bias during DJF(JJA) season compared to ERA5 HREs that show occurrence of 50 % in DJF and 18 % in JJA. Furthermore, we examined the weather pattern and anomalies that led to anomalous conditions of the season specific HREs in Davao River basin, which showed MRI-AGCM3.2S was able to reproduce the general structure of anomalous conditions fairly well on both seasons in comparison with ERA5. However, the slight over(under)estimation of the surface anomalous conditions in DJF(JJA) are directly proportional to the over(under)estimation in rainfall magnitude in the basin.

Inadequacy of spatio-temporal hydro-climatic data limits the efficacy of hazard monitoring and disaster risk reduction activities in disaster-prone areas. Various satellite missions are recently providing climate data, but prior evaluation and enhancement of these data are necessary for a reliable application. In this study, we conducted performance evaluation and enhancement of three real-time satellite precipitation products (SPPs) (GSMaP, GPM-IMERG, and PERSIANN) for flood modeling in the Blue Nile basin. The bias correction improved the original SPPs, with the largest improvement being for factors generated from 10 d mean data. Flood event hydrograph indicated satisfactory results of error metrics on the devastating flood event of 2012. Employing reliable physical–based distributed hydrologic models provided longer lead time and high-accuracy flood simulation. Furthermore, the results indicate that integrating available initial observed precipitation data improved the efficiency of SPPs simulation, and hence are applicable in operational flood monitoring.

Two new negative-parity bands have been identified in the odd-odd 104Rh nucleus. According to their experimentally observed properties, they have the same π(1g9/2)−1⊗ν(1h11/2)1 high-j configuration as the previously known negative-parity chiral doublet bands. This observation raises the possibility of the existence of multiple chiral doublet bands, MχD, in this nucleus. Comparing the properties of the observed bands with results of detailed theoretical calculations, one can conclude that the lower energy parts of bands 1 and 2 are chiral partner bands with the π(1g9/2)−1⊗ν(1h11/2)1 two-quasiparticle configuration, while bands 3 and 4 are chiral partner bands with the π(1g9/2)−1⊗ν(1g7/2)−2(1h11/2)1 four-quasiparticle configuration. Thus, MχD based on different configurations is observed in 104Rh.

Until the early 1990s, cyst nematodes were abundant pathogens in fields where hosts of Heterodera spp. were frequent members of crop rotations along California’s Central Coast. To mitigate damage caused by Heterodera schachtii and H. cruciferae, the soil fumigant 1,3-dichloropropene (1,3-D) was used by more than 43% of surveyed broccoli growers. Over the last few decades, use of 1,3-D and other nematicides has dramatically diminished, suggesting a decline in nematode disease pressure. The goal of this study was to examine the hypothesis that increased population densities of nematophagous fungi contribute to the low populations of Heterodera spp. in fields frequently cropped to their hosts. In 2016, soil samples were collected from 152 Brassica fields with a broad geographical distribution, from Santa Barbara County to Santa Cruz County. The average number of Heterodera cysts per 250 cm3 of soil ranged from 0.5 to 27.5, with 62% of the soils harboring no detectable cyst nematodes and only a few samples reaching a potentially damaging threshold level. A baiting experiment with H. schachtii and cabbage was performed in a greenhouse to detect nematophagous fungi associated with nematode females as their posterior end emerged and became exposed to the soil’s rhizosphere. An Illumina-based sequence analysis of these H. schachtii females identified several known nematophagous fungi, including members of the Hyalorbilia oviparasitica clade, Pochonia chlamydosporia, certain Fusarium spp., and others. These soils clearly harbor a diverse population of hyperparasitic fungi that could be biologically suppressing cyst nematodes below a damaging threshold.[Figure: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

Allyl isothiocyanate (AITC) is a glucosinolate produced in cruciferous plant species. AITC is known to act as a pesticide on microorganisms, insects, and weeds. Synthetic AITC is registered as a biopesticide for agricultural soil treatment use in the United States and elsewhere in the world. Although a potent pesticide, reports on the weed and pathogen control efficacy of synthetic AITC applied as soil disinfectant are highly variable. Due to the low vapor pressure of AITC, questions remain as to whether pest and weed control efficacy can be improved by combining it with other chemicals. The objective of this study was to assess the control efficacy of AITC stand-alone applications vs. applications, in which AITC was combined with the standard-fumigants chloropicrin, 1,3-dichloropicrin, and methyl isothiocyanate. Two shank-applied on-farm field trials were conducted in cut flower [delphinium (Delphinium elatum), ranunculus (Ranunculus asiaticus)] fields, and two drip tape applied field trials in strawberry (Fragaria ×ananassa) fields in California. Weed pressure, weed seed viability, nematode survival, and pathogen survival of Pythium ultimum, fusarium wilt (Fusarium oxysporum), and verticillium wilt (Verticillium dahliae) were assessed. Cumulative yearly yield of marketable fruit was assessed in the strawberry field trials. The results of this study show that the use of AITC as a stand-alone treatment provided no consistent weed or pathogen control efficacy. However, our results also indicate that shank and drip applied multitactic fumigation approaches with AITC can efficiently control soil-borne diseases and weeds. These findings have potential implications, especially in those areas where certain fumigants are restricted due to regulations and/or availability.

Although deciduous and evergreen conifers are expected to utilize needle nitrogen differently, their interspecific differences in the ozone sensitivity remain unclear. We compared the growth and physiological responses to elevated O3 concentrations in deciduous Japanese larch (Larix kaempferi) and evergreen Sakhalin fir (Abies sachalinensis) seedlings. Seedlings of both species were exposed to 62.5 ± 1.95 nmol(O3) mol-1 during the day time in open-top chambers. Elevated O3 had no effect on height or diameter growth in either of the species but suppressed photosynthetic parameters. A decline in photosynthetic nitrogen-use efficiency was observed in both species. However, needle N content in larch was decreased by O3, whereas it was not decreased in fir. Needle N responses to O3 were different between larch and fir seedlings, indicating that the O3 sensitivity of photosynthesis could vary depending on needle N dynamics. We also observed a higher correlation between photosynthesis and needle N even under O3 exposure, particularly in the mass-based relationship with higher accuracy. The result indicated that mass-based needle N could explain difference in O3 sensitivity of photosynthesis between the different leaf habits in larch and fir seedlings.

The low-lying dipole strength in the 90,94Zr nuclei was investigated via (p,p′γ) at 80 MeV and (α,α′γ) at 130 MeV. The experiments, made at RCNP, used the magnetic spectrometer Grand Raiden for the scattered particles and the array CAGRA with HPGe detectors for the γ-decay. For 94Zr these are the first data for both reactions and for 90Zr these are the first data with (p,p′γ) and the first ones at high resolution for (α,α′γ). The comparison of the present results for the two nuclei with existing (γ,γ′) data shows that both nuclear probes produce an excitation pattern different than that of the electromagnetic probes.DWBA calculations were made using form factors deduced from transition densities, based on RPA calculations, characterized by a strong neutron component at the nuclear surface. A combined analysis of the two reactions was performed for the first time to investigate the isoscalar character of the 1− states in 90,94Zr. The (p,p′γ) cross section was calculated using values for the isoscalar electric dipole energy-weighted sum rule (E1 ISEWSR) obtained from the (α,α′γ) data. The isoscalar strength for 90Zr was found to exhaust 20 ± 2.5% of the EWSR in the energy range up to 12 MeV. In case of 94Zr, a strength of 9 ± 1.1% of the EWSR was found in the range up to 8.5 MeV.Although an overall general description was obtained in the studied energy intervals, not all proton cross sections were well reproduced using the isoscalar strength from (α,α′γ). This might suggest mixing of isoscalar and isovector components and that this mixing and the degree of collectivity are not the same for all the 1− states below the particle binding energy.

The activity of commercial soil fumigants on some key soilborne pathogens was assessed in sandy loam soil under controlled conditions. Seven soil fumigants that are registered in California or are being or have been considered for registration were used in this study: dimethyl disulfide (DMDS) mixed with chloropicrin (Pic) (79% DMDS and 21% Pic), Tri-Con (50% methyl bromide and 50% Pic), Midas Gold (33% methyl iodide [MI] and 67% Pic), Midas Bronze (50% MI and 50% Pic), Midas (MI, active ingredient [a.i.] 97.8%), Pic (a.i. 99% trichloronitromethane) and Pic-Clor 60 (57% Pic and 37% 1,3-dichloropropene [1–3,D]). Dose-response models were calculated for pathogen mortality after 24 hours of exposure to fumigants. Overall, the tested fumigants achieved good efficacy with dosages below the maximum label rate against the tested pathogens. In this study, Pythium ultimum and citrus nematode were sensitive to all the fumigants and Verticillium dahliae was resistant. For most fumigants, California regulations restrict application rates to less than the maximum (federal) label rate, meaning that it is possible that the fumigants may not control major plant pathogens. This research provides information on the effectiveness of these alternatives at these lower application rates. The results from this study will help growers optimize application rates for registered fumigants (such as Pic and 1,3-D) and will help accelerate the adoption of new fumigants (such as DMDS) if they are registered in California.

Adequate estimation of the spatial distribution of snowfall is critical in hydrologic modelling. However, this is a well-known problem in estimating basin-scale snowfall, especially in mountainous basins with data scarcity. This study focuses on correction and estimation of this spatial distribution, which considers topographic effects within the basin. A method is proposed that optimises an altitude-based snowfall correction factor (Cfsnow). This is done through multi-objective calibration of a spatially distributed, multilayer energy and water balance-based snowmelt model (WEB-DHM-S) with observed discharge and remotely sensed snow cover data from the Moderate Resolution Imaging Spectroradiometer (MODIS). The Shuffled Complex Evolution–University of Arizona (SCE–UA) automatic search algorithm is used to obtain the optimal value of Cfsnow for minimum cumulative error in discharge and snow cover simulations. Discharge error is quantified by Nash–Sutcliffe efficiency and relative volume deviation, and snow cover error was estimated by pixel-by-pixel analysis. The study region is the heavily snow-fed Yagisawa Basin of the Upper Tone River in northeast Japan. First, the system was applied to one snow season (2002–2003), obtaining an optimised Cfsnow of 0.0007 m−1. For validation purposes, the optimised Cfsnow was implemented to correct snowfall in 2004, 2002 and 2001. Overall, the system was effective, implying improvements in correlation of simulated versus observed discharge and snow cover. The 4 yr mean of basin-average snowfall for the corrected spatial snowfall distribution was 1160 mm (780 mm before correction). Execution of sensitivity runs against other model input and parameters indicated that Cfsnow could be affected by uncertainty in shortwave radiation and setting of the threshold air temperature parameter. Our approach is suitable to correct snowfall and estimate its distribution in poorly gauged basins, where elevation dependence of snowfall amount is strong.

In this study, the snow physics of a distributed biosphere hydrological model, referred to as the Water and Energy Budget based Distributed Hydrological Model (WEB-DHM) is significantly improved by incorporating the three-layer physically based energy balance snowmelt model of Simplified Simple Biosphere 3 (SSiB3) and the Biosphere-Atmosphere Transfer Scheme (BATS) albedo scheme. WEB-DHM with improved snow physics is hereafter termed WEB-DHM-S. Since the in-situ observations of spatially-distributed snow variables with high resolution are currently not available over large regions, the new distributed system (WEB-DHM-S) is at first rigorously tested with comprehensive point measurements. The stations used for evaluation comprise the four open sites of the Snow Model Intercomparison Project (SnowMIP) phase 1 with different climate characteristics (Col de Porte in France, Weissfluhjoch in Switzerland, Goose Bay in Canada and Sleepers River in USA) and one open/forest site of the SnowMIP phase 2 (Hitsujigaoka in Japan). The comparisons of the snow depth, snow water equivalent, surface temperature, snow albedo and snowmelt runoff at the SnowMIP1 sites reveal that WEB-DHM-S, in general, is capable of simulating the internal snow process better than the original WEB-DHM. Sensitivity tests (through incremental addition of model processes) are performed to illustrate the necessity of improvements over WEB-DHM and indicate that both the 3-layer snow module and the new albedo scheme are essential. The canopy effects on snow processes are studied at the Hitsujigaoka site of the SnowMIP2 showing that the snow holding capacity of the canopy plays a vital role in simulating the snow depth on ground. Through these point evaluations and sensitivity studies, WEB-DHM-S has demonstrated the potential to address basin-scale snow processes (e.g., the snowmelt runoff), since it inherits the distributed hydrological framework from the WEB-DHM (e.g., the slope-driven runoff generation with a grid-hillslope scheme, and the flow routing in the river network).

In this study, a frozen soil parameterization has been modified and incorporated into a distributed biosphere hydrological model (WEB-DHM). The WEB-DHM with the frozen scheme was then rigorously evaluated in a small cold area, the Binngou watershed, against the in-situ observations from the WATER (Watershed Allied Telemetry Experimental Research). First, by using the original WEB-DHM without the frozen scheme, the land surface parameters and two van Genuchten parameters were optimized using the observed surface radiation fluxes and the soil moistures at upper layers (5, 10 and 20 cm depths) at the DY station in July. Second, by using the WEB-DHM with the frozen scheme, two frozen soil parameters were calibrated using the observed soil temperature at 5 cm depth at the DY station from 21 November 2007 to 20 April 2008; while the other soil hydraulic parameters were optimized by the calibration of the discharges at the basin outlet in July and August that covers the annual largest flood peak in 2008. With these calibrated parameters, the WEB-DHM with the frozen scheme was then used for a yearlong validation from 21 November 2007 to 20 November 2008. Results showed that the WEB-DHM with the frozen scheme has given much better performance than the WEB-DHM without the frozen scheme, in the simulations of soil moisture profile at the cold regions catchment and the discharges at the basin outlet in the yearlong simulation.

An optical particle counter (OPC) is used in conjunction with lidar measurements to examine the characteristics of the particle size distribution in cirrus cloud in the tropical tropopause layer (TTL) over Thailand where the TTL is defined as the height at which temperature is lower than −75°C in this paper. Of 11 OPC launches, cirrus cloud was detected at 10–15 km high on 7 occasions, cirrus was detected in the TTL in 6 cases, and simultaneous OPC and lidar measurements were made on two occasions. Comparison of lidar and OPC measurements reveal that the cloud heights of cirrus in the TTL varies by several hundred meters over distances of tens kilometers; hence the height is not always horizontally uniform. The mode radii of particles constituting the clouds are estimated by lidar and OPC measurements to be less than approximately 10 μm. The regression lines of the particle size distribution with and without cirrus cloud exhibit similar features at equivalent radii of

Use of cover crops and compost increased soil quality in irrigated, intensive production of lettuce and broccoli in the Salinas Valley. These methods had the beneficial impacts of increasing soil microbial biomass, increasing total soil carbon and nitrogen, reducing surface bulk density and decreasing the potential for groundwater pollution as a result of nitrate leaching below the root zone. These soil benefits did not lead to lower yields and occasionally resulted in fewer weeds and lower lettuce corky root disease. Although surface minimum tillage reduced yields, it led to reduced potential for nitrate leaching below the root zone. Use of conventional tillage, cover crops, and compost produced high vegetable yields and acceptable net economic returns over a 2-year period, but broccoli was more profitable than lettuce under this regime. Understanding the trade-offs of various costs and benefits will help growers choose management practices that optimize economic and environmental benefits.

The finding of Phytophthora ramorum — the pathogen that causes sudden oak death in four California native trees — on rhododendron in Europe led us to hypothesize that its host range in California's natural forests was much greater than previously suspected. In addition to the affected oak species, we have now identified an additional 13 species from 10 plant families that act as hosts for P. ramorum in California. Our data indicates that nearly all of the state's main tree species in mixed-evergreen and redwood-tanoak forests — including the coniferous timber species coast redwood and Douglas fir — may be hosts for P. ramorum. The broad host range of P. ramorum, the variability of symptoms among different hosts and the ability of the pathogen to disperse by air suggests that it may have the potential to cause long-term, landscape-level changes in California forests.

The superconducting fluctuations above critical temperature in the Bi2Sr2Ca1-xYxCu2O8 single crystals are studied. The boundaries of the superconducting fluctuations area are defined by the MWA measurement. The estimation of the fluctuations lifetimes is made.

Glucose-6-phosphate dehydrogenase (G6PD) is the key enzyme of the pentose phosphate pathway in carbohydrate metabolism, and it plays an important role in cell proliferation and antioxidant regulation within cells in various organs. Although marked cell proliferation and oxidant/antioxidant metabolism occur in lung alveolar epithelial cells, definite data has been lacking as to whether cytochemically detectable G6PD is present in alveolar epithelial cells. The distribution pattern of G6PD within these cells, if it is present, is also unknown. The purpose of the present study was to investigate the subcellular localization of G6PD in alveolar cells in the rat lung using a newly- developed enzyme-cytochemistry (copper-ferrocyanide) method. Type I cells and stromal endothelia and fibroblasts showed no activities. Electron-dense precipitates indicating G6PD activity were clearly visible in the cytoplasm and on the cytosolic side of the endoplasmic reticulum of type II alveolar epithelial cells. The cytochemical controls ensured specific detection of enzyme activity. This enzyme may play a role in airway defense by delivering substances for cell proliferation and antioxidant forces, thus maintaining the airway architecture.

Adequate estimation of the spatial distribution of snowfall is critical in hydrologic modeling. However, this is a well-known problem in estimating basin-scale snowfall, especially in mountainous basins with data scarcity. This study focuses on correction and estimation of this spatial distribution, which considers topographic effects within the basin. A method is proposed that optimizes an altitude-based snowfall correction factor (Cfsnow). This is done through multi-objective calibration of a spatially distributed, multilayer energy and water balance-based snowmelt model (WEB-DHM-S) with observed discharge and remotely sensed snow cover data from the Moderate Resolution Imaging Spectroradiometer (MODIS). The Shuffled Complex Evolution -- University of Arizona automatic search algorithm is used to obtain the optimal value of Cfsnow for minimum cumulative error in discharge and snow cover simulations. Discharge error is quantified by Nash--Sutcliffe efficiency and relative volume deviation, and snow cover error was estimated by pixel-by-pixel analysis. The study region is the heavily snow-fed Yagisawa Basin of the Upper Tone River in northeast Japan. First, the system was applied to one snow season (2002-2003), obtaining an optimized Cfsnow of 0.0007m-1. For validation purposes, the optimized Cfsnow was implemented to correct snowfall in 2004, 2002 and 2001. Overall, the system was effective, implying improvements in correlation of simulated vs. observed discharge and snow cover. The 4 yr mean of basin average snowfall for the corrected spatial snowfall distribution was 1160mm (780mm before correction). Execution of sensitivity runs against other model input and parameters indicated that Cfsnow could be affected by uncertainty in shortwave radiation and setting of the threshold air temperature parameter. Our approach is suitable to correct snowfall and estimate its distribution in poorly-gauged basins, where elevation depen dence of snowfall amount is strong. [ABSTRACT FROM AUTHOR]

Abstract  Photosynthetic water use efficiency (PWUE), stomatal conductance (g s), and water potential were measured at two different positions in the tree crown of two emergent tropical tree species (Shorea beccariana Burck, Dryobalanops aromatica Gaertn. f.). The trees were about 50 m high, in a tropical rain forest in Sarawak, East Malaysia. In both species, g s at the upper crown position at midday was lower than at the lower crown position, even though both positions were exposed to full sunlight; the difference was greater in S. beccariana. Hydraulic limitation occurs in the upper crown position in both species. A midday depression was observed in the photon saturated photosynthetic rate in both species, especially at the upper crown. However, PWUE was markedly higher in the upper crown than the lower crown at midday, even though no morphological adjustment was observed in the leaves; this difference was greater in S. beccariana. [ABSTRACT FROM AUTHOR]

Abstract??We investigated the responses of photon-saturated photosynthesis rate (Psat) and its simultaneous acclimation of anatomy and nitrogen use patterns of current needles of Korean pine (Pinus koraiensis) seedlings grown under factorial combinations of two nitrogen levels and irradiances. Although N supply resulted in a significant increase of N content in needles under both irradiances, the increase ofPsattended to be suppressed only in shade (S). The significant increase ofPsatin full sunlight (O) was associated with the increase of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) and chlorophyll (Chl) contents. In contrast, small increase of Chl content and no increase of RuBPCO content were found in S (90 % cut of full irradiance), which would result in a small increase ofPsat. This result suggests that extra N is stocked in needles under shade for the growth in next season. With N supply, a significant decrease of specific leaf area (SLA) was detected only in O. This decrease of SLA was due to the increase of density of needle. Furthermore, the increase of needle density was not due to the increased number and size of mesophyll cells, but the increased density of each mesophyll cell. Therefore, although SLA changed in O, the change did not involve anatomical adaptation to use increased N effectively, at least observable by light microscopy. Hence, even though the SLA would change, N deposition will improve the photosynthetic capacity of Korean pine seedlings, not through the development of needle anatomy but through improvement of the allocation of N in both irradiances. [ABSTRACT FROM AUTHOR]

Beginning in 2000 and continuing into 2004, a previously undescribed disease caused significant damage to romaine lettuce (Lactuca saliva) in the coastal Salinas Valley of California. Symptoms were brownish black, sunken cavities on the crown and upper taproot. Cavities were firm, lacked signs of fungal growth, and resulted in cracking and weakening of the crown. Affected plants were stunted, uneven in growth, and unmarketable. Crisphead lettuce also developed these symptoms, although disease incidence was always lower than that for romaine. A fungus was consistently isolated from cavities. Using morphological features and molecular methods, the fungus was identified as Phoma exigua. Pathogenicity of isolates from romaine and crisphead lettuce was demonstrated on both of these lettuce types. Replicated field studies showed that azoxystrobin, boscalid, and cyprodinil + fludioxonil fungicides effectively controlled the disease. This is the first report of a crown disease caused by P exigua in the United States. A similar disease, Phoma basal rot on greenhouse grown lettuce in the United Kingdom, had been previously observed, but no report has been published. [ABSTRACT FROM AUTHOR]

A stem-girdling experiment was carried out on an evergreen conifer, the Korean pine (Pinus koraiensis Sieb. et Zucc.), in mid summer in Northeast China. A 50 % higher respiration rate at the upper part of the stem was observed 3 d after stem girdling, and a stable higher rate (1.2–2.8 times) one week later. However, no higher soluble sugar or starch contents were found in the upper bark of the girdled stems in measurements over three weeks. These findings indicate that most of the newly-formed photosynthates were consumed by the high respiratory activity; this is also implied by the strong correlation between the photosynthetic photon flux over the canopy (PPF) and respiration at the upper parts of girdled stems. Moreover, the maximum PPF and cumulative PPF one day before measurement (PPFmax-Y and CPPF-Y, respectively) were closely correlated with the respiratory difference between the upper and the lower parts, but no such correlation was found with the instantaneous PPF (PPF-I) and cumulative PPF on the current day from sunrise to measured time point (CPPF-C). This shows that photosynthates newly formed by canopy needles need at least one day for transportation in order to increase the stem respiration at tree breast height. [ABSTRACT FROM AUTHOR]

Objective: An extensive study as to whether maternal age itself is a risk factor for blood loss during parturition. Method: A total of 10,053 consecutive women who delivered a singleton infant were studied.The excess blood loss was defined separately for women with vaginal and cesarean deliveries as >= 90th centile value for each delivery mode. The effects of 13 potential risk factors on blood loss were analyzed using multivariate analysis. Results: The 90th centile value of blood loss was 615 ml and 1,531 ml for women with vaginal and cesarean deliveries, respectively. A low lying placenta (odds ratio [OR], 4.4), previous cesarean (3.1), operative delivery (2.6), leiomyoma (1.9), primiparity (1.6), and maternal age >= 35 years (1.5) were significant independent risk factors for excess blood loss in women with vaginal delivery. Placenta previa (6.3), leiomyoma (3.6), low lying placenta (3.3), and maternal age >= 35 years (1.8) were significant independent risk factors for excess blood loss in women with cesarean sections. Conclusion: A maternal age of >= 35 years was an independent risk factor for excess blood loss irrespective of the mode of delivery, even after adjusting for agerelated complications such as leiomyoma, placenta previa, and low lying placenta. [ABSTRACT FROM AUTHOR]

Glucose metabolism through the pentose phosphate pathway (PPP) seems to play a critical role in meiotic resumption in mouse oocytes (Downs et al. 1998 Biol. Reprod. 58, 1084–1094). However, the role is not clear in porcine oocytes. In the present study, we examined whether glucose affects morphological change of germinal vesicles and the resumption of meiosis in porcine oocytes in a chemically defined medium. In the first experiment, porcine cumulus–oocyte complexes (COCs) were collected from 3–6-mm follicles of slaughterhouse ovaries and cultured in a chemically defined medium, mNCSU37-PVA with/without 5.55 mM glucose in the presence of eCG, hCG, and dibutyryl cAMP for 20–22 h and then in the absence of eCG, hCG, and dibutyryl cAMP for 24 h. In the second experiment, 5.55 mM glucose in the maturation medium was replaced with the same concentration of Na pyruvate. In the third experiment, the PPP inhibitor 6-aminonicotinamide (6-AN) was added to the maturation medium at various concentrations (0, 10, 50, and 100 M). To determine the activity of glucose-6-phosphate dehydrogenase (G6PD), OCCs were fixed, blocked, and treated with anti-G6PD polyclonal antibody and the secondary antibody labeling a fluorescent material. Results from 3–5 replicates were analyzed by ANOVA and Duncan''s multiple range test. When OCCs were cultured in glucose-free chemically defined maturation media, regardless of the presence of hormones and dibutyryl cAMP, germinal vesicle breakdown (GVBD) of oocytes was inhibited (10.0–21.3%), as compared with OCCs cultured in the presence of glucose and hormones (91.4–92.0%). In a majority of oocytes in which GVBD was inhibited, the arrest occurred at the GV-I stage. When OCCs were cultured in maturation media in which glucose was replaced with Na pyruvate, GVBD was not inhibited any more than in control samples that were cultured in the presence of glucose (97.4% vs. 97.1%). However, the incidence of oocytes developing to the metaphase II stage was significantly lower in this condition than in controls (4.8% vs. 49.9%, respectively). A majority of the oocytes were at the metaphase I stage (86.0% vs. 45.5% in controls). The presence of 6-AN in maturation media significantly inhibited GVBD of oocytes (77.3, 29.0, 7.4, and 8.4% at 0, 10, 50, and 100 µM, respectively) and arrested the oocytes at the GV-I stage. Immunocytochemistry with anti-G6PD demonstrated the activity of G6PD in cumulus cells of OCCs. In conclusion, these results demonstrate that glucose plays a critical role in the release of porcine oocytes arrested at the GV-I stage, probably through PPP of cumulus cells. The current results also suggest the possibility of gluconeogenesis in porcine OCCs when glucose in maturation media was replaced with Na pyruvate. [ABSTRACT FROM AUTHOR]

Data related to radioactivity released from the Fukushima Daiichi Nuclear Power Plant (FDNPP) accident on 15 March 2011 gathered by residents of Miharu, Fukushima Prefecture, and by Tohoku University are presented. These data sets consist of (1) the earliest radiation monitoring by a Geiger counter in the town, (2) ratios of radioactivity between 132Te and 137Cs for a wide area between Fukushima and Tokyo, (3) radiation measurement of soil samples collected from 18 school grounds, and (4) external radiation exposure of 1400 students using OSL badges. By combining and analysing these various data sets, a curve for the cumulative total external exposure as a function of time, with 16 : 00 h on 15 March 2011 being time zero, is obtained. The average cumulative external dosage is estimated to be 10 mSv (σ = 4.2 mSv) over 10 years. In addition, the initiative that the residents of Miharu took in response to the FDNPP accident, which became known as The Misho Project (MP), is documented; in particular, the time at which the municipality instructed the immediate ingestion of iodine tablets by those under the age of 40, 13 : 00 h on 15 March 2011, is assessed. [ABSTRACT FROM AUTHOR]

Objectives. Haemophagocytic syndrome (HPS) is known as a relatively rare complication in autoimmune diseases. Here we analysed the clinical features of HPS in patients with systemic autoimmune diseases. Methods. One thousand and fourteen patients with systemic autoimmune diseases admitted to Hokkaido University Hospital from 1997 to 2007 were recruited [350 SLE, 136 RA, 98 polymyositis/dermatomyositis (PM/DM), 88 SSc, 91 vasculitis syndrome, 37 primary SS, 26 adult onset Stills disease (AOSD) and 188 other diseases]. Clinical features and treatment outcomes were retrospectively analysed. Results. Thirty cases (3.0%) fulfilled HPS criteria (progressive cytopenia in two or more lineages and haemophagocytosis in reticuloendothelial systems). Underlying diseases were SLE (18), RA (2), PM/DM (2), SSc (2), vasculitis (1), SS (2) and AOSD (3). Nineteen patients were diagnosed as having autoimmune-associated HPS, eight infection-associated, one drug-induced and one developed HPS after haematopoietic stem cell transplantation. For the treatment of HPS, high-dose corticosteroid monotherapy was given in 26 cases, being effective in 12 (46%). Ten out of 15 patients with corticosteroid-resistant autoimmune-associated HPS were treated with CsA, cyclophosphamide or tacrolimus, leading to the remission in 80%. The overall mortality rate was 20%. Multivariate analysis showed that the presence of infections and CRP level >50 mg/l on HPS related with poor prognosis. Conclusions. The prevalence of HPS among in-hospital patients with systemic autoimmunity is not ignorable. Administration of immunosuppressants was effective in cases with autoimmune-associated HPS, whereas prognosis was poor in infection-associated HPS. [ABSTRACT FROM AUTHOR]

Oral propranolol (OP) remains the first-line treatment for problematic infantile hemangiomas (IHs). However, the efficacy of OP for IH varies among patients, with reports suggesting that some lesions are resistant to OP. Therefore, monitoring methods for accurate evaluation are particularly important issue, especially during the OP introduction period. Dermoscopy, a noninvasive and simple technique for various skin disorders, can occasionally be used for the longterm evaluation of OP treatment. We present five cases of patients with IHs who were started on OP treatment and monitored using a dermoscopic camera during the titolation period. In all lesions, a very early reaction to OP could be detected. Involution was noted 4 days after OP initiation in four lesions, and after 1 week in one lesion. Changes were more obvious in the dermoscopic images than in clinical images. Reduction of erythema was the main change and was apparent at the edge of the lesion. Dermoscopy might be superior to other assessment modalities given its ability for the detection of the early detailed changes in response to OP. [ABSTRACT FROM AUTHOR]

NLRP3 inflammasome inhibitor is a highly attractive drug target for the treatment of various inflammatory diseases. Here, we report the discovery of pyridazine derivatives as a new class of scaffold for NLRP3 inflammasome inhibitors. We optimized HTS hit 2a to improve both in vitro IL-1β inhibitory activity and the mean photo effect (MPE) value in the in vitro 3T3 neutral red uptake (NRU) phototoxicity test. As a result, we identified compound 5e (ASP0965) with brain penetrability and showing efficacy in the brain on oral administration in the rat pharmacodynamics (PD) model and the mouse α-synuclein injection model. These findings suggest that compound 5e is a promising clinical candidate for α-synucleinopathy therapeutics., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Mesenchymal glioma stem cells (MES-GSCs) are a major subtype of GSCs that reside within glioma tissues and contribute to metastasis, therapy resistance, and glioma recurrence. However, the precise molecular mechanisms governing MES-GSC functions remain elusive. Our findings revealed that expression levels of miR-505-5p/-3p are elevated in MES-GSCs compared with those in proneural (PN)-GSCs, glioma cell lines, and normal brain tissue and that miR-505-5p/-3p expression levels are decreased in differentiated MES-GSCs. We assumed that miR-505-5p/-3p would play distinctive roles in MES-GSCs and performed loss-of-function experiments targeting miR-505-5p/-3p. Knockdown of miR-505-5p/-3p increased proliferation and promoted differentiation in MES-GSCs while suppressing invasion, temozolomide resistance, and enhancing apoptosis in MES-GSCs. Mechanistically, miR-505-5p/-3p directly targets the 3' UTR of AUF1, leading to its repression in MES-GSCs. Notably, AUF1 expression levels were significantly lower in MES-GSCs compared with those in PN-GSCs, glioma cell lines, and normal brain tissues. Co-inhibition of AUF1 expression with miR-505-5p/-3p knockdown ameliorated the observed cellular phenotypes caused by miR-505-5p/-3p knockdown in MES-GSCs. These results suggest that miR-505-5p/-3p exerts MES-GSC-specific roles in regulating proliferation, differentiation, invasion, apoptosis, and temozolomide resistance by repressing AUF1 expression., (© 2024 Wiley Periodicals LLC.)

This study introduces a novel class of carbon-centered diradicals: a monosubstituted C-atom stabilized by a phosphine. The diradical Ph3P→C was photochemically generated from a diazophosphorus ylide precursor (Ph3PCN2) and characterized by EPR and isotope-sensitive ENDOR spectroscopy at low temperatures. Ph3P→C features an axial zero-field splitting parameter D = 0.543 cm-1 with a vanishingly small rhombicity |E|/D = 0.002. Time- and temperature-dependent measurements confirm a triplet ground state with a lifetime of approximately 10 min at 127 K in toluene-d8. Multireference electronic structure calculations predict a clear triplet ground state with a singlet-triplet gap greater than 20 kcal/mol. In contrast to divalent C(0) compounds, such as Ph3P→C←PPh3, in which carbon needs excitation into a highly-excited closed-shell 2s02p4 configuration, Ph3P→C can be explained by direct involvement of carbon in its natural 3P state arising from the 2s22p2 configuration., (© 2025 Wiley‐VCH GmbH.)

Classic Hodgkin lymphoma (CHL) histologically consists of Hodgkin Reed-Sternberg (HRS) cells and the tumor microenvironment (TME), but the relationship between TME characteristics and clinical features of CHL remains unclear. We aimed to investigate the effects of the TME structure on the outcomes of patients with CHL. We performed a high-throughput analysis of HRS cells and their topological relationship with the reactive immune cells in the TME. After multiplexed immunofluorescence labeling against CD4, CD8, CD30, CD68, CD163, PD-1, and PD-L1, visual images were analyzed. Phenotypes were assigned to all reactive cells, such as CD4 + and CD8 + T-cells and macrophages. Since the densities of PD1 + /CD4 + T-cells, CD8 + T-cells, and PD-L1 + macrophages were significantly higher in the area < 60 μm than in the area < 120 μm from each HRS cell in 45 tissue samples from 34 patients with CHL, we further analyzed the TME-component cells by focusing on the 60 μm radius in the initial samples. TMEs containing > 15 CD8 + T-cells were associated with a significantly better 3-year progression-free survival than those with ≤ 15 CD8 + T-cells (100% vs. 53%, p = 0.006). In comparison with TMEs containing ≤ 15 CD8 + T-cells, TMEs containing > 15 CD8 + T-cells had significantly more PD-L1 - macrophages (mean 3 vs. 1 cell, p = 0.015) and fewer PD-1 + /CD4 + T-cells (mean 16 vs. 28 cells, p = 0.036). Epstein-Barr virus positivity in HRS cells was significantly associated with a higher number of macrophages in the 60 μm radius area. In conclusion, the TME structure in patients with CHL can differ, enabling precision therapies., Competing Interests: Declarations. Ethical approval: This study was approved by the Nihon University Itabashi Hospital Clinical Research Judging Committee in accordance with the Ethical Guidelines for Medical and Health Research Involving Human Subjects (updated March 2023; approval number RK-210608-9). The committee waived the requirement for informed consent from the patients owing to the retrospective nature of this study. Competing interests: H.T. received honoraria from Chugai, Kyowa Kirin, Nippon Shinyaku, Takeda, and Bristol-Myers Squibbs. T.H. received honoraria from Bristol-Myers Squibb. M. N. received honoraria from Bristol-Myers Squibb. K.M. received honoraria from AstraZeneca, Chugai, Kyowa Kirin, Takeda, Bristol-Myers Squibb, Nippon Shinyaku, SymBio, and Ono Parma. H.N. has received research grants from MSD, Asahi Kasei Pharma, Astellas, AbbVie, Japan Blood Products Organization, Eisai, Otsuka Pharmaceutical, Ono Pharma, Kyowa Kirin, Sanofi, Shionogi, Daiichi Sankyo, Taiho, Takeda, Mitsubishi Tanabe, Chugai, Teijin Pharma, Eli Lilly, Nippon Kayaku, Nihon Pharmaceutical, Boehringer Ingelheim, and Pfizer. The authors declare no other conflicts of interest., (© 2025. The Author(s).)

Stereoselective synthesis of β-fluorostyrene derivatives has been achieved. Selective isomerization of gem -bromofluoroalkenyl benzenes bearing various ortho -substituents is enabled by using Ir photocatalysts with high triplet energy. Subsequent one-pot transition-metal (TM)-catalyzed reactions enable pot-economical synthesis of monofluoroalkenes in a stereoselective manner.

Skeletal muscles contain lipids inside and outside cells, namely intramyocellular lipids (IMCL) and extramyocellular lipids (EMCL), respectively; lipids have also been found to be interspersed between these muscles as adipose tissue, namely intermuscular adipose tissue (IMAT). Metabolized IMCL has been recognized as an important substrate for energy production and their metabolism is determined by the muscle oxidative capacity. Therefore, it has been speculated that muscle oxidative capacity is related to muscle lipid content. Excessive accumulation of EMCL and IMAT has been confirmed in older individuals, leading to metabolic disorders and a decline in muscle strength. However, whether EMCL and IMAT contribute to muscle lipid metabolism remains unknown. This study aimed to investigate whether muscle oxidative capacity is related to IMCL, EMCL, and IMAT in young and older individuals. A total of 18 young and 14 older individuals were included and their muscle oxidative capacity was assessed based on the recovery rate of muscle oxygen saturation after exercise, using near-infrared spectroscopy of the medial gastrocnemius. IMCL, EMCL, and IMAT were assessed using magnetic resonance spectroscopy and imaging. A relationship between muscle oxidative capacity and IMCL was confirmed in young (r=-0.47, P<0.05) but not older individuals (r=0.22, P=0.45). Muscle oxidative capacity was not related to EMCL or IMAT in either young or older individuals. These results suggest that IMCL in young individuals can contribute to muscle lipid metabolism, but not EMCL and IMAT, and this relationship differs with aging.

Empirical studies worldwide show that warming has variable effects on plant litter decomposition, leaving the overall impact of climate change on decomposition uncertain. We conducted a meta-analysis of 109 experimental warming studies across seven continents, using natural and standardised plant material, to assess the overarching effect of warming on litter decomposition and identify potential moderating factors. We determined that at least 5.2° of warming is required for a significant increase in decomposition. Overall, warming did not have a significant effect on decomposition at a global scale. However, we found that warming reduced decomposition in warmer, low-moisture areas, while it slightly increased decomposition in colder regions, although this increase was not significant. This is particularly relevant given the past decade's global warming trend at higher latitudes where a large proportion of terrestrial carbon is stored. Future changes in vegetation towards plants with lower litter quality, which we show were likely to be more sensitive to warming, could increase carbon release and reduce the amount of organic matter building up in the soil. Our findings highlight how the interplay between warming, environmental conditions, and litter characteristics improves predictions of warming's impact on ecosystem processes, emphasising the importance of considering context-specific factors., (© 2024 The Author(s). Ecology Letters published by John Wiley & Sons Ltd.)

Background: The patients taking direct oral anticoagulants (DOACs) are at high risk for developing ischemic stroke and delayed bleeding in upper gastrointestinal endoscopic submucosal dissection (ESD). We aimed to identify the optimal DOAC based on both adverse events in upper gastrointestinal ESD., Methods: A retrospective population-based cohort study was conducted using the Diagnosis Procedure Combination database in Japan. We included patients on a DOAC undergoing upper gastrointestinal ESD between 2012 and 2021. The primary outcomes were ischemic stroke occurring after upper gastrointestinal ESD and delayed bleeding in gastroduodenal and esophageal ESD. Inverse probability weightings were applied to balance the four DOAC groups (dabigatran, rivaroxaban, apixaban, and edoxaban), and logistic regression analyses were performed to compare the outcomes., Results: We analyzed 9729 patients on a DOAC undergoing upper gastrointestinal ESD. Ischemic stroke developed after upper gastrointestinal ESD in 1.4%, 0.7%, 0.6%, and 0.8% of patients taking dabigatran, rivaroxaban, apixaban, and edoxaban, respectively, after weighting. Rivaroxaban and apixaban showed significantly lower risk of ischemic stroke compared with dabigatran (odds ratio, 0.15 and 0.12, respectively) in standard doses. The delayed bleeding developed after gastroduodenal ESD in 7.6%, 14.6%, 19.2%, and 17.3% of patients taking each DOAC, respectively, with the lowest risk in dabigatran, followed by rivaroxaban. A similar pattern was observed in delayed bleeding in esophageal ESD (3.2%, 5.4%, 7.5%, and 5.5% in each DOAC), but with no significant results., Conclusions: Rivaroxaban might be an optimal DOAC for upper gastrointestinal ESD showing a lower risk for both ischemic stroke and delayed bleeding., Competing Interests: Declarations. Conflict of interest: All authors have no conflicts of interest to be declared. Patient consent for publication: Not required., (© 2024. The Author(s).)

CD34 is a well-known cell marker of hematopoietic stem/ progenitor cells, endothelial cells, and fibrocytes. In the peripheral nervous system, a certain type of primary sensory neuron C-fiber low threshold mechanoreceptors (C-LTMRs) are reported to express CD34 mRNA. Here, we investigated the distribution of CD34 protein among putative C-LTMRs (pC-LTMR) using pC-LTMR markers such as VGLUT3 and TH in the dorsal root ganglion (DRG) and spinal cord. CD34 was frequently observed in DRG neurons double-positive for VGLUT3 and TH and single-positive for VGLUT3 in C8 and L4 levels, however, in C4 and L1 levels most of CD34-positive DRG neurons were demonstrated to be double-positive for VGLUT3 and TH. As for the termination, CD34-positive DRG neurons terminated in the ventral part of inner lamina II (lamina IIiv). At C4 and L1 levels of the dorsal horn, CD34 was observed in the entire region of lamina IIiv, however, in C8 and L4 levels of the dorsal horn CD34 was not detected in the medial part of lamina IIiv, which receives neural inputs from DRG neurons that innervate palm or sole skin. These results indicate that CD34 is expressed in pC-LTMRs and suggest that CD34 may play a role in providing C-LTMRs with a specific sensation by maintaining neural circuits., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Desmoid fibromatosis (DF) is defined as a borderline tumor of the soft tissues with a low malignant potential. The most common tumor sites are the extremity, trunk, abdominal cavity, and head and neck. Surgical resection has been the standard treatment for DF. However, there are concerns regarding long-term cosmetic outcomes or functional morbidity associated with surgery particularly in the head and neck. Recently, the use of the front-line wait-and-see strategy and pharmacologic treatment such as chemotherapy for progression has been proposed as tumors can spontaneously stabilize and regress. Herein, we report the efficacy of vinblastine (VBL) and methotrexate (MTX) in a childhood patients with DF. A 21-month-old female patient with a 4-cm tumor at the left lower jaw was diagnosed with DF. She did not initially receive any therapies (wait-and-see) according to the recent treatment guidelines. However, the tumor gradually progressed, and the patient was managed with COX2 inhibitor. Since the tumor was not controlled with such a treatment and tracheal exclusion caused by the mass was a cause of concern, the patient was managed with chemotherapy with VBL and MTX. VBL and MTX were administered weekly for 26 weeks, every other week, and further for 26 weeks. The tumor size gradually decreased with VBL and MTX. Magnetic resonance imaging revealed no evidence of the disease at the end of chemotherapy. Good cosmetic outcomes were achieved, and recurrence was not observed after 24 months of follow-up.

Objective . Classification models for electroencephalogram (EEG) data show a large decrease in performance when evaluated on unseen test subjects. We improve performance using new regularization techniques during model training. Approach . We propose several graphical models to describe an EEG classification task. From each model, we identify statistical relationships that should hold true in an idealized training scenario (with infinite data and a globally-optimal model) but that may not hold in practice. We design regularization penalties to enforce these relationships in two stages. First, we identify suitable proxy quantities (divergences such as Mutual Information and Wasserstein-1) that can be used to measure statistical independence and dependence relationships. Second, we provide algorithms to efficiently estimate these quantities during training using secondary neural network models. Main results . We conduct extensive computational experiments using a large benchmark EEG dataset, comparing our proposed techniques with a baseline method that uses an adversarial classifier. We first show the performance of each method across a wide range of hyperparameters, demonstrating that each method can be easily tuned to yield significant benefits over an unregularized model. We show that, using ideal hyperparameters for all methods, our first technique gives significantly better performance than the baseline regularization technique. We also show that, across hyperparameters, our second technique gives significantly more stable performance than the baseline. The proposed methods require only a small computational cost at training time that is equivalent to the cost of the baseline. Significance . The high variability in signal distribution between subjects means that typical approaches to EEG signal modeling often require time-intensive calibration for each user, and even re-calibration before every use. By improving the performance of population models in the most stringent case of zero-shot subject transfer, we may help reduce or eliminate the need for model calibration., (© 2024 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved.)

Background: Even in current guideline-directed medical therapy, including recently introduced vasopressin type 2 receptor antagonist tolvaptan, congestion has not been resolved in patients with heart failure. Kampo medicine goreisan has been receiving considerable attention as an additional therapy for patients who are refractory to conventional diuretics therapy, including tolvaptan. However, the impact of goreisan on urine electrolytes remains uncertain. Methods: Patients with congestive heart failure who received goreisan as an add-on therapy to tolvaptan-incorporated medical therapy were prospectively included. The changes in urine parameters during the first 24 h were assessed as a primary concern. Baseline factors associated with an increase in urine sodium excretion were investigated. Results: A total of 21 patients were included. The median age was 81 (77, 86), and 13 (62%) were men. Twenty-four hours after the initiation of goreisan, urine osmolality decreased significantly, urine sodium level remained unchanged, urine potassium and glucose levels decreased significantly, urine urea nitrogen level tended to decrease, and urine volume tended to increase. The fractional excretion of sodium tended to increase. Baseline plasma B-type natriuretic peptide level had a positive correlation with a change in fractional excretion of sodium from baseline to day 1 (r = 0.52, p = 0.015). Conclusions: Goreisan may increase urine volume via aquaretic and natriuretic effects in patients with congestive heart failure receiving tolvaptan-incorporated medical therapy. Goreisan may have the ability to "modulate" fluid balance depending on congestion status.

Background: The Esophageal Hypervigilance and Anxiety Scale (EHAS) is an English questionnaire created in the USA to assess these factors in all patients with esophageal diseases. The aim of this study was to develop and validate the Japanese version of EHAS and investigate the relationship between EHAS scores and symptoms in untreated disorders of esophagogastric junction (EGJ) outflow., Methods: This prospective study recruited patients who underwent high-resolution manometry (HRM) at six tertiary centers in Japan. The EHAS was translated to Japanese using standard forward and backward translation methods. Patients completed the following questionnaires: the Japanese EHAS, Eckardt score, Gastroesophageal Reflux Disease Questionnaire, and Hospital Anxiety and Depression Scale for assessment of construct validity. Logistic regression analysis identified factors associated with esophageal symptom severity in untreated disorders of EGJ outflow., Results: Overall, we analyzed 432 patients. Their main symptoms were dysphagia and reflux. The most common HRM diagnosis was normal (35.9%), followed by achalasia (29.4%). The Japanese EHAS demonstrated excellent reliability, and construct validity, with two subscales similar to the original EHAS. Total EHAS score moderately correlated to Eckardt score (r = 0.545, p < 0.001). In 113 patients with untreated disorders of EGJ outflow, multivariable analysis demonstrated that younger age, type II achalasia, and higher EHAS score were independently associated with higher Eckardt score., Conclusions: The Japanese EHAS is a reliable and valid questionnaire. Its subscale scores can be used as in the original version with some caution. Future studies are warranted to assess the appropriateness of factor loading., Competing Interests: Declarations. Conflict of interests: Akinari Sawada: Bristol Myers Squibb (grant).,Daniel Sifrim: Jinshan (speaking, grant, trips), Reckitt Benckiser (speaking, grant, trips).,Tiffany H. Taft: Ayble Health (scientific advisory board), Oak Park Behavioral Medicine LLC (ownership).,John Erik Pandolfino: Medtronic (speaking, consulting, patent, license), Sandhill Scientific/Diversatek (grant), Takeda (speaking), Astra Zeneca (speaking), Torax/Ethicon (speaking, consulting), EndoGastric Solutions (advisory board), Phathom (speaking, consulting).,Yasuhiro Fujiwara: Takeda (speaking), Astra Zeneca (speaking), Astellas (speaking), DaiichiSankyo (speaking), Otsuka (speaking), EA Pharma (speaking).The remaining authors declare no conflicts of interest in this study., (© 2024. The Author(s).)