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Turfgrasses are measured aesthetically and by their ability to withstand stressors. Historically, researchers quantified acceptability by visual quality, but inconsistencies necessitate the use of vegetation indices (VIs) as an objective measurement. Indiscernible relationships have been established between turfgrass canopy normalized difference vegetation index (NDVI) and important variables such as soil moisture and leaf chlorophyll content. Alternative and variable-specific indices have been established in cropping systems. The water band index (WBI) is used for predicting water availability in cropping systems but has not been explored within turfgrass systems. The objective of this study was to compare the relationships of established VIs with chlorophyll content of 'L-93' creeping bentgrass (Agrostis stolonifera L.) and water content of a sand-based root zone maintained under greenhouse conditions. All VIs were moderately to strongly correlated to total chlorophyll content (r = 0.49-0.85). Turf color was more closely related to chlorophyll indices than to wet-lab quantification. Only WBI (r = 0.80) and the green/red ratio index (GRI; r = 0.50) were consistently related to soil water content. The NDVI was weakly related to soil water content in one trial (r = 0.49). Nonlinear regression showed that WBI can be useful for estimating a decline in soil water content as water first becomes limiting for creeping bentgrass and may offer a viable means to assess water availability independently of non-moisture-related stresses and more accurately compared with previous indices. Future research will evaluate WBI on broader geospatial scales to assess practical application for turfgrass irrigation management. [ABSTRACT FROM AUTHOR]

Traffic simulators are often utilized when researching turfgrass wear tolerance and recovery. However, the availability of a durable traffic simulator capable of producing dynamic force is limited. Therefore, the objectives of this research were to: (i) evaluate a novel traffic simulator with improved durability and capable of producing dynamic force and (ii) evaluate the biophysical effects of the traffic simulator on a native soil turfgrass system. The Baldree traffic simulator is a modified Ryan GA 30 (Jacobsen, A Textron Company, Charlotte, NC) riding aerification unit, equipped with fabricated, spring loaded steel plate feet studded with screw in cleats. The effects of this unit on a 'Tifway' bermudagrass [Cynodon dactylon (L.) Pers. × C. transvaalensis Burtt-Davy] system established on a Tifton loamy sand (fine-loamy, kaolinitic, thermic Plinthic Kandiudults) were evaluated at the Coastal Plains Experiment Station, Tifton, GA. Factors included location (1 and 2) and traffic rate (0, 12, and 24 passes applied over a 6 wk period). Field data included soil bulk density, turf density, and percent green turf cover. An in-ground force plate at the McPhail Equine Performance Center, East Lansing, MI was used to quantify vertical and net shear ground reaction force produced by the Baldree traffic simulator when operated in the forward and backward direction. The Baldree traffic simulator produced more cleat marks per pass than the Brinkman and Cady traffic simulators. At the low traffic rate the Baldree traffic simulator increased soil bulk density, while turf density and percent green turf cover decreased; therefore, the tool can be used to simulate heavy traffic conditions with a minimum number of passes. [ABSTRACT FROM AUTHOR]

Management zones (MZs) for southern root-knot nematode (RKN) from the integration of terrain (TR) and edaphic (ED) field features might facilitate variable rate nematicide applications. This study was conducted on 11 coastal plain fields in the USA. The relationships between RKN populations and five soil ED and TR attributes (apparent soil electrical conductivity [shallow (ECa-s) and deep (ECa-d)], elevation (EL), slope (SL), and changes in bare soil reflectance) were analyzed using canonical correlation. Using two ED and TR data sets, canonical predictors were used for zone delineation. Although the results showed that the zones with RKN population above the RKN field average were associated with the lowest values of ECa-s, ECa-d, normalized difference vegetation index (NDVI), and SL with respect to field average values, zone segregation was enough using ECa-s and ECa-d data. The results suggest the potential for using soil properties to identify RKN risk zones. [ABSTRACT FROM AUTHOR]

Vegetation indices based solely on visible reflectance may simplify and decrease the cost of crop growth estimates compared to visible and nearinfrared (NIR) indices. Ground-based and aerial visible and visible/NIR vegetation indices based on aerial images were compared for sensitivity to ground cover fraction (GCF) of cotton (Gossyplum hirsutum L.) under four irrigation treatments in 2004 and five treatments in 2005 and 2006. Inseason cotton imagery was collected using an unmodified Nikon COOLPIX 4300 camera and a COOLPIX 4300 camera modified for NIR imaging attached to a tethered blimp. GCF imagery was collected at 45 to 60 m and compared with normalized difference vegetation index (NDVI) and green/red ratio values from imagery collected at 180 to 250 m. Ground-based (1.5 m) spectrometer NDVI measurements using multiple spectral regions were also evaluated. Spectrometer (r² = 0.40 to 0.80) and camera (r² = 0.68 to 0.90) indices were highly correlated with season-wide GCF between fractions of 0.20 and 0.80 and were sensitive to irrigation treatments. Camera green/red ratio was linearly correlated with GCF throughout the 3 yr. The pooled comparison for the 3 yr was strongly linear (r² = 0.86). Our results suggest that the green/red ratio index might allow quick, simple, and accurate crop growth estimates for production. [ABSTRACT FROM AUTHOR]

The Conservation Effects Assessment Program Watershed Assessment Study is a joint effort between the USDA Natural Resources Conservation Service (NRCS) and the USDA Agricultural Research Service to evaluate the effectiveness of federally funded conservation programs. In response to this initiative, a 26-year history of NRCS conservation practice placement (1980 to 2006) was evaluated for the Little River Experimental Watershed (LREW) in the southeastern coastal plain of Georgia. To accomplish this task, currently available geographic databases were integrated and queried to assess levels of commonly adopted practices and to evaluate factors affecting practice placement. Databases included (1) USDA NRCS Conservation Practice Database for the LREW, (2) USDA NRCS Soil Survey Geographic Database (SSURGO), and (3) 30 m (98 ft) digital elevation maps. Nearly 50% of all cropland fields in the LREW were delineated as having participated in conservation programs. Practices were predonsinantly used for water quality and erosion control. Sixty to 65% of the fields (77% of land area) implemented soil erosion and/or water quality control practices in high resource concern areas. Results showed that hydrologic group and proximity to a water body, rather than slope class, were the predominant factors in conservation practice placement. Using a subwatershed database having complete field coverage of four LREW subwatersheds (with and without USDA NRCS assistance), geographic information system databases were queried to evaluate the adoption and placement of erosion control practices that were visible in a 2005 digital orthoquad. Forty-seven percent of all fields in the subwatershed database had implemented visible erosion control-specific conservation practices. and implementation was linearly related to slope class (r² = 0.64, p < 0.10). Fields identified as having participated in federally funded conservation programs coincided with high resource concern areas 35% of the time. [ABSTRACT FROM AUTHOR]

The article discusses a study which evaluates the impact of nonionic surfactant on cotton production on a loamy sand in the coastal plain region of Georgia. The experiment was composed of on-site rainfall simulation and agronomic cotton field trials. Rainfall simulation discovered that the addition of surfactant increased runoff and decreased infiltration. It also found that agronomic field trials showed that crop yields were not different between surfactant-treated and untreated plots.

Scientists with the U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS) and scientists with the University of Georgia located at the Coastal Plain Experiment Station in Tifton, Georgia, have been conducting research on aflatoxin contamination of peanut since the early 1960s. Early efforts were focused on identifying the risk factors for increased aflatoxin contamination and helped to document the importance of drought, high soil temperatures, and pod damage. Later efforts were focused on the development of screening techniques and the identification of sources of resistance to Aspergillus colonization and/or aflatoxin contamination. This laid the foundation for a conventional resistance breeding program and has resulted in the development of peanut breeding lines that have high yield and low aflatoxin contamination relative to standard control cultivars. Recent research efforts include studies on the use of molecular genetic approaches to reduce aflatoxin contamination. This includes the evaluation of genetically engineered peanut and the development of molecular markers. [ABSTRACT FROM AUTHOR]

Abstract: Conservation tillage is a commonly adopted best management practice for improving soil quality and reducing erosion. However, there are currently no methods in place to monitor conservation tillage adoption at the watershed scale. The primary objective of this study was to evaluate the usefulness of Landsat TM data as a tool to depict conservation tillage in a small Coastal Plain watershed. Satellite imagery was used to calculate four commonly used indices: Normalized Difference Vegetation Index, Crop Residue Cover Index, Normalized Difference Tillage Index, and the Simple Tillage Index. Ground truth data consisted of a windshield survey, assigning each site a tillage regime (conventional or conservation tillage) at 138 locations throughout the watershed and surrounding areas. A logistical regression approach was used on two subsets of the data set (n = 20 or n = 44) to determine the influence of the number of ground control points on the success of modeling the occurrence of Conservation tillage. The most accurate model was re-applied to the satellite image and evaluated using an independent sample of 94 survey sites. Results indicate that the normalized difference tillage and simple tillage indices performed best, with an overall accuracy of 71% and 78% for models developed using n = 20 and n= 44 sample locations, respectively. Errors were typically in the form of commission. Results are encouraging and suggest that currently available satellite imagery can be used for rapid assessment of conservation tillage adoption using minimal a priori information. [ABSTRACT FROM AUTHOR]

Conservation tillage is a well known best management practice that improves soil quality, reduces runoff and erosion, and increases infiltration. However, a rapid assessment strategy for quantifying the rate and spatial distribution of conservation tillage practices is lacking. This study was designed to evaluate the sensitivity of a remotely derived crop residue cover index for depicting conventional tillage (CT), strip tillage (ST), and no-tillage (NT). systems in a cotton-corn-peanut rotation in tile southeastern Coastal Plain. Treatments consisted of CT (rip and bed operation), NT, NT with subsoiling, and STE. Remotely sensed data were acquired three times prior to canopy closure, using a handheld multispectral radiometer (485 to 1,650 nm) and thermal imager (7,000 to 14,000 nm). Using a combination of visible and near-infrared spectra, a crop residue cover index was calculated and evaluated. Results showed that crop residue cover is greatest in years, planted with peanut or cotton--likely due to the later winter cover crop termination date compared to years when corn is planted. The. crop residue cover index outperformed the thermal infrared, accurately separating conventional from conservation tillage treatments in four out of six data acquisitions in 2004 and 2006. Differentiation among conservation tillage treatments was inconsistent. Regression analyses showed that a strong linear relationship existed between tile crop residue cover index and measured crop residue cover (r² = 0.51 to 0.86, alpha = 0.10).These data suggest that remotely sensed data may be used as a rapid, field-scale indicator of conservation tillage adoption. Rapid assessment methodologies are necessary to quantify the impact of conservation practice adoption on Water quality/quantity, assess adoption rates, and improve the placement of conservation tillage practices at local, watershed and regional scales. [ABSTRACT FROM AUTHOR]

Airborne thermal infrared (TIR) imagery is a promising and innovative tool for assessing canopy response to a range of stressors. However, the expense associated with acquiring imagery for agricultural management is often cost-prohibitive. The objective of this study was to evaluate a less expensive system, an unmanned airvehicle (UAV) equipped with a TIR sensor, for detecting cotton (Gossypium hirsutum L.)response to irrigation and crop residue management. The experimental site was located on a 6.1 ha field in the Tennessee Valley Research and Extension Center located in Belle Mina, Alabama, where landscapes are gently rolling and soils are highly weathered Rhodic Paleudults. Treatments consisted of irrigation (dryland or subsurface drip irrigation) and crop residue cover (no cover or winter wheat (Triticum aestivum L.)). TIR (7 to 14 µm) imagery was acquired on 18 July 2006 at an altitude of 90 m and spatial resolution of O.5 m. Coincident with image acquisition, ground truth data consisting of soil water content (0-25 cm), stomatal conductance, and canopy cover were measured within a 1 m radius of each sample location. All sample locations were georeferenced using a real-time kinematic (RTK) GPS survey unit. Analysis of sample locations acquired in multiple flight lines was used to assess the stability and repeatability of the UAV system during an acquisition. Compared to field measurements of stomatal conductance with CVs ranging from 2% to 75%, variability in TIR emittance (CV < 40%) was within the observed tolerance of ground truth measurements of stomatal conductance. Significant differences in canopy cover and stomatal conductance across irrigation treatments allowed testing of the sensitivity of the UAV system. A negative correlation was observed between TIR emittance and stomatal conductance (r = -0.48) and canopy closure (r = -0.44), indicating increasing canopy stress as stomatal conductance and canopy closure decreased. TIR emittance exhibited greater sensitivity to canopy response compared to ground truth measurements, differentiating between irrigation and crop residue cover treatments. TIR imagery acquired with a low-altitude UAV can be used as a tool to manage within-season canopy stress. [ABSTRACT FROM AUTHOR]

The U.S. Department of Agriculture Agricultural Research Service Southeast Watershed Research Laboratory (SEWRL) initiated a hydrologic research program on the Little River Experimental Watershed in south-central Georgia, United States, in 1967. The primary intent of the program was to develop an improved understanding of basic hydrologic and water quality processes on Coastal Plain watersheds and to evaluate the effects of agricultural management practices on the region's natural resources and environment. Long-term (up to 37 years), research-quality streamflow data have been collected for up to eight flow measurement sites within the Gulf-Atlantic Coastal Plain physiographic region, an important agricultural production area in the southeastern United States. Forty-six precipitation gauges and three climate stations are currently in operation to collect data in support of the hydrologic network. Over the past 20 years, sediment and agrichemical concentrations in streamflow have also been monitored to permit evaluation of the impacts of agriculture on regional surface and groundwater quality. Along with the hydrologic and water quality data, geographic spatial data layers for terrain, soils, geology, vegetation, and land management have also been developed. These databases, described in five accompanying data reports, can be accessed via an ftp site supported by the SEWRL (ftp://www.tiftonars.org/). [ABSTRACT FROM AUTHOR]

The Little River Experimental Watershed located in the headwaters of the Upper Suwannee River basin is one of twelve national benchmark watersheds participating in the U.S. Department of Agriculture (USDA) Conservation Effects Assessment Project-Watershed Assessment Studies (CEAP-WAS). Historical paper files and maps (circa 1980-2006) were collected and used to develop a geographic database of conservation practices supported by the USDA Natural Resource Conservation Service. The CEAP-WAS database can be queried by conservation practice, total acreages enrolled, year of implementation, and location. The CEAP-WAS database is integral to understanding the links between conservation practice implementation and placement with observed changes in hydrologic processes within a small southern Coastal Plain watershed. All associated geographic information has been provided in shapefile format and has been projected into universal transverse Mercator coordinates (zone 17), using NAD83 as the datum and GRS80 as the ellipsoid. Data may be accessed via ftp://www.tiftonars.org/, archived in a folder named ceap_data. [ABSTRACT FROM AUTHOR]

The Little River Experimental Watershed is located in the headwaters of the Upper Suwannee River basin and is one of twelve national benchmark watersheds participating in the U.S. Department of Agriculture Conservation Effects Assessment Project-Watershed Assessment Studies. A geographic database has been established to include topography, land use, hydrology, soil distribution, watershed boundaries, and site locations for all weirs, rain gauges, soil moisture sites, and climate stations. These data provide the foundation for integrating point-based measurements with landscape attributes. Each spatial layer can be accessed individually for use within a geographic information system. The watershed boundary layer will serve as the base map, projected into universal transverse Mercator coordinates (zone 17), using NAD83 as the datum and GRS80 as the ellipsoid. Data may be accessed via ftp://www.tiftonars.org/. [ABSTRACT FROM AUTHOR]

Reduced tillage and surface residue increases infiltration, soil water content, and plant available water, while at the same time decreasing runoff and sedimentation. However, there is a general lack of knowledge and appreciation regarding the impact conservation tillage has on sustainable water resources. The objective of this study was to estimate water savings as a result of conservation tillage adoption in Georgia. Total acreages by crop (cotton, corn, and peanut) and tillage (conventional and conservation) were obtained via the Conservation Technology Information Center for the 2004 growing season. Rainfall simulation studies conducted over row-cropped lands in conventional and conservation tillage were obtained for soils in the Coastal Plain and Piedmont physiographies. Data were integrated within a geographical information system. In 2004, cotton, corn, and peanuts represented 85% of row crop production in Georgia, with nearly 90% of the acreage in the Coastal Plain. Conservation tillage systems are currently in place on approximately 30% of those acreages, primarily in the form of strip tillage. Results from rainfall simulation studies indicate that conservation tillage can reduce runoff and increase infiltration in these systems by 29% to 46%. Extrapolating these results to the state, conservation tillage reduced estimated statewide, irrigated water requirements from 4% to 14%. Increasing conservation tillage to 40% in intensively row-cropped counties where conservation tillage adoption rates were less than the national average (40%) increased estimated water savings by an additional 1% to 6%. [ABSTRACT FROM AUTHOR]

Conservation programs developed and implemented by the USDA have led to land-use changes of large areas throughout the U.S. These changes in land-use may lead to changes in evapotranspiration and infiltration and subsequently to dramatic differences in hydrologic response. The impact of recent land-use changes was evaluated using observed precipitation and streamflow data from the Little River watershed in south-central Georgia in the U.S. Land-use patterns within the watershed were, examined through classification of satellite images collected from 1975 to 2003. While some changes in land-use were determined, analysis of the data indicated that the overall changes were less than the typical classification errors obtained. Based on the analysis of the satellite imagery, conservation practices implemented in the Little River watershed have not significantly altered total forest acreage. It addition, 34 years of hydrologic data collected from this watershed do not indicate any significant changes in hydrologic patterns. The long-term average ratio of annual flow to annual precipitation for the Little River watershed has remained stable at approximately 0.27. Year-to-year variation of the ratio varied from a high of 0.41 observed during a year with above-normal winter rainfall to 0.06 observed during a year with very low annual rainfall. When subwatersheds of the Little River were compared, significant differences in their responsiveness to rainfall were found. Linear regression between precipitation and streamflow produced regression coefficients between 0.62 and 0.90. These differences were attributed to differences in physical characteristics and land-use. Long-term data from the watersheds indicate that streamflow response is dominated by annual and seasonal precipitation characteristics. [ABSTRACT FROM AUTHOR]

Transformations and losses of nitrogen (N) throughout the growing season can be costly. Methods in place to improve N management and to facilitate split N applications during the growing season can be time consuming and logistically difficult. Remote sensing (RS) may be a method to rapidly assess temporal changes in crop N status and to promote more efficient N management. This study was designed to evaluate the ability of three different RS platforms to predict N variability in corn (Zea mays L.) leaves during vegetative and early reproductive growth stages. Plots (15 × 15 m) were established in the Coastal Plain (CP) and in the Appalachian Plateau (AP) physiographic regions each spring from 2000 to 2002 in a completely randomized design. Treatments consisted of four N rates (0, 56, 112, and 168 kg N ha−1) applied as ammonium nitrate (NH4NO3) replicated four times. Spectral measurements were acquired via spectroradiometer (λ = 350–1050 nm), Airborne Terrestrial Applications Sensor (ATLAS) (λ = 400–12,500 nm), and the IKONOS satellite (λ = 450–900 nm). Spectroradiometer data were collected on a biweekly basis from V4 through R1. Due to the nature of satellite and aircraft acquisitions, these data were acquired per availability. Chlorophyll meter (SPAD) and tissue N were collected as ancillary data, along with each RS acquisition. Results showed vegetation indices derived from hand-held spectroradiometer measurements as early as V6–V8 were linearly related to yield and tissue N concentration. The ATLAS data were correlated with tissue N at the AP site during the V6 stage (r2 = 0.66), but no significant relationships were observed at the CP site. No significant relationships were observed between plant N and IKONOS imagery. By using a combination of the greenness vegetation index and the normalized difference vegetation index, RS data acquired via ATLAS and the spectroradiometer could be used to evaluate tissue N variability and to estimate corn yield variability given ideal growing conditions. [ABSTRACT FROM AUTHOR]

Crop residues influence near surface soil organic carbon (SOC) content, impact our ability to remotely assess soil properties, and play a role in global carbon budgets. Methods that measure crop residues are laborious, and largely inappropriate for field-scale to regional estimates. The objective of this study was to evaluate high spectral resolution remote sensing (RS) data for rapid quantification of residue cover. In March 2000 and April 2001, residue plots (15 by 15 m) were established in the Coastal Plain and Appalachian Plateau physiographic regions of Alabama. Treatments consisted of five wheat (Triticum aestivum L.) straw cover rates (0, 10, 20, 50, and 80%) replicated three times. Spectral measurements were acquired monthly via a handheld spectroradiometer (350-1050 nm) and per availability via the Airborne Terrestrial Applications Sensor (ATLAS) (400-12 500 nm). Overall, treatment separation was influenced by soil water content and percentage of total organic carbon (TOC) of the residue (degree of decomposition). Results showed that atmospherically corrected visible and near-infrared ATLAS data can differentiate between residue coverages. Similar results were obtained with the handheld spectroradiometer, although treatment differentiation was less consistent. Thermal infrared ATLAS imagery best discriminated among residue treatments due to differing heat capacities between soil and residue. Results from our study suggest airborne thermal infrared (TLR) imagery can be used for crop residue variability assessment within the southeastern USA. [ABSTRACT FROM AUTHOR]

Although conservation tillage is widely believed to be an agricultural management practice effective for increasing soil carbon (C) accretion and associated soil quality, there is limited research to determine whether conservation tillage increases net C accretion versus simply altering the distribution of C content by soil depth. We implemented conservation farming practices (winter cover cropping plus strip tillage) for a nonirrigated corn (Zea mays L.) production system in the southeastern coastal plain of Georgia, United States, that had been previously managed under a conventional plow and harrow tillage regime. Total soil C and nitrogen (N) were measured on samples collected from 0 to 65 cm (0 to 25.6 in) at 57 sites before and after five years under conservation farming practices. Crop yield, winter and summer aboveground crop biomass production, and biomass C and N content were also measured annually at each site. Soil C increased an average of 20 Mg ha-1 (8.9 tn ac-1; 6 to 62 Mg C ha-1 [2.6 to 27.6 tn C ac-1], depending upon slope position) and was associated with a N increase of 2 Mg ha-1 (0.89 tn ac-1). Although 72% to 80% of the C accretion was in the top 35 cm (13.8 in), 3 to 6 Mg C ha-1 (1.3 to 2.6 tn C ac-1) was accreted from 35 to 65 cm (13.8 to 25.6 in). The soil C accreted during the study amounted to 36% of the net biomass C produced. Corn yield increased 2,200 kg ha-1 (1,964 lb ac-1) depending upon slope position (1,200 to 2,500 kg ha-1 [1,071 to 2,232 lb ac-1]) during the same time. Analysis indicated that soil C content from 15 to 35 cm (5.9 to 13.8 in) was the soil parameter primarily associated with corn yield. Season rainfall from planting to corn silking stage for both corn production years was the lowest in the past 45 years (20 to 25 cm [7.8 to 9.8 in] below the net crop demand) suggesting that soil C-mediated increase in plant-available soil water was a mechanism contributing to improved corn yield. Calculated estimates (from soil clay, sand, and C content) of increased soil water holding capacity suggest that C accretion in the top 35 cm (13.8 in) of soil potentially increased water storage enough to supply up to four days' worth of additional crop water demand. These results indicated that conservation farming practices can increase soil C and N accretion in degraded sandy soils of the humid southeastern United States coastal plain, and that increased soil C may potentially mitigate the deleterious effects of short-term rainfall deficits in nonirrigated production systems. [ABSTRACT FROM AUTHOR]

The watershed-scale effects of agricultural conservation practices are not well understood. A baseline calibration and an input parameter sensitivity analysis were conducted for simulation of watershed-scale hydrology in the Little River Experimental Watershed (LREW) in the Coastal Plain near Tifton, Georgia. The Soil and Water Assessment Tool (SWAT) was manually calibrated to simulate the hydrologic budget components measured for the 16.9 km² subwatershed K of the LREW from 1995 to 2004. A local sensitivity analysis was performed on 16 input variables. The sum of squares of the differences between observed and simulated annual averages for baseflow, stormflow, evapotranspiration, and deep percolation was 19 mm²; average annual precipitation was 1136 mm. The monthly Nash-Sutcliffe model efficiency (NSE) for total water yield (TWYLD) was 0.79 for the ten-year period. Daily NSE for TWYLD was 0.42. The monthly NSE for three years with above-average rainfall was 0.89, while monthly NSE was 0.59 for seven years with below annual average rainfall, indicating that SWAT's predictive capabilities are less well-suited for drier conditions. Monthly average TWYLD for the high-flow winter to early spring season was underpredicted, while the low-flow late summer to autumn TWYLD was overpredicted. Results were negatively influenced when seasonal tropical storms occurred during a dry year The most sensitive parameters for TWYLD were curve number for crop land (CN2(crop)), soil available water content (SOL_AWC), and soil evaporation compensation factor (ESCO). The most sensitive parameters for storm flow were CN2(crop), curve number for forested land (CN2(forest)), soil bulk density (SOL_BD), and SOL_AWC. The most sensitive parameters for baseflow were CN2(crop), CN2(forest), ESCO, and SOL_AWC. Identification of the sensitive SWAT parameters in the LREW provides modelers in the Coastal Plain physiographic region with focus for SWAT calibration. [ABSTRACT FROM AUTHOR]

Dense dendritic stream networks in the Tifton Upland (southeastern U.S. coastal plain) provide an opportunity to determine the effects of land management practices on individual farms on downstream hydrology and water quality. A typical farm will be drained by two or three small streams. The streams may be bordered by riparian forests or impounded into farm ponds. Two adjacent farm-scale basins, both of which were 50 to 60 ha (124 to 148 ac) in size were compared for seven years to determine the effects of upstream agricultural land uses, downstream riparian zones, and small impoundments on stream water quality and hydrology. Stream water quality was sampled at four points on the two basins and at a downstream outlet where the two streams came together in a farm pond. The north basin had more cropland than the south basin and had much more area in plastic-covered beds for vegetable production. The south basin had less land in crop production and much more of the total basin area in farm ponds used to supply irrigation water. The north basin had more surface runoff and higher loads of all nutrients and sediment. The south basin had only 55% of the total runoff of the north basin, probably due to the presence of about 6% of the total watershed area in farm ponds. Up to 26% of the north basin (38% of total cropland) was occupied by wide plastic-covered beds in the last two years of the study. Large quantities of sediment transported in surface runoff from these fields on the north basin led to increases in sediment concentrations and loads of over 100 times compared to both the south basin and to earlier years of the study. Based on reductions in differences in loads and concentrations between upstream and downstream sites, there is less potential to reduce nutrients and sediment once these materials are in stream flow than when water is moving to streams through a riparian buffer. A downstream pond that received inputs from both the north and south basins had significantly lower concentrations of most nutrients and sediments than either of the upstream sampling sites. [ABSTRACT FROM AUTHOR]

Hepatitis C virus (HCV) infection is very common among chronic hemodialysis patients. In the past, blood transfusion appeared to be the primary risk factor; however evidence of nosocomial HCV transmission in the hemodialysis setting has recently been reported. This report describes a molecular investigation of HCV isolates obtained from a population of 670 patients attending six different Seattle-King County based hemodialysis centers in order to identify potential common source infections. 733 serum specimens were collected from hemodialysis patients in 1992 and 1996, and were tested for HCV antibodies and RNA. Overall, 115 of 670 (17%) patients were positive for HCV RNA, and thus were considered actively infected by HCV. HCV genotype was determined in all cases by restriction fragment length polymorphism, and 93 patients were found to be infected by HCV genotype 1. HCV envelope genes were amplified from the 93 patients with genotype 1 infection, and were studied in further detail by heteroduplex tracking analysis (HTA) using genotype 1a and 1b specific probes derived from the envelope 1 (E1) and envelope 2 (E2) genes. Genetic relatedness between pairs of HCV envelope genes was estimated by calculating the degree of gel shift relative to homoduplex controls. Nucleotide sequencing and phylogenetic analysis was used to confirm genetic relatedness detected by HTA. When HTA was performed using the E1 gene probe, 12 apparently related infections were detected; 10 of 12 (83%) of these infections were confirmed as truly related using the gold standard method of nucleotide sequencing plus phylogenetic analysis. Using an E2 gene probe, 24 infections were apparently related, but only six (25%) were confirmed by sequencing. As a control, 41 envelope genes, which were unrelated by HTA, were sequenced; 0 of 41 (0%) were truly related. In summary, HTA provides a rapid and effective molecular technique for screening HCV genetic relatedness in population-based studies, and should prove valuable in future studies of HCV molecular epidemiology.

Background: Hepatitis C virus infection persists after liver transplantation and causes recurrent liver injury in the majority of patients. Standard dose interferon therapy has been largely unsuccessful for hepatitis C in transplant recipients., Methods: Twelve patients, at least 7 months posttransplant, with detectable hepatitis C virus RNA in serum and features of hepatitis C on liver biopsy were randomized to interferon-alpha2a, 3 mU daily for 12 months (n=8) or no treatment (n=4). The tolerability of daily interferon dosing in liver transplant recipients was evaluated and effects on hepatitis C virus RNA level, quasispecies evolution, and liver histology were studied., Results: Treated patients had an improvement in histological activity index at the end of therapy relative to controls (median reduction of 2 versus median increase of 1.5) (P=0.04). Four treated patients had a virological response (all bDNA negative, one qualitative polymerase chain reaction negative) compared with none of the untreated patients. Only two of six treated patients tested had evidence of quasispecies diversification on therapy. Seven of eight patients in the treatment group required dose reduction for fatigue and/or depression. They tolerated 1.5 mU of interferon-alpha2a daily. Two treated patients developed graft dysfunction, one of who had histological evidence of rejection and subsequent graft loss., Conclusions: Low daily doses of interferon were tolerated by liver transplant recipients and provided histological benefit without associated quasispecies diversification in most cases. These findings provide a rationale to study low dose daily or pegylated interferon maintenance therapy for the management of hepatitis C posttransplant.

The hepatitis C virus (HCV) nonstructural 5A (NS5A) protein has been controversially implicated in the inherent resistance of HCV to interferon (IFN) antiviral therapy in clinical studies. In this study, the relationship between NS5A mutations and selection pressures before and during antiviral therapy and virologic response to therapy were investigated. Full-length NS5A clones were sequenced from 20 HCV genotype 1-infected patients in a prospective, randomized clinical trial of IFN induction (daily) therapy and IFN plus ribavirin combination therapy. Pretreatment NS5A nucleotide and amino acid phylogenies did not correlate with clinical IFN responses and domains involved in NS5A functions in vitro were all well conserved before and during treatment. A consensus IFN sensitivity-determining region (ISDR(237-276)) sequence associated with IFN resistance was not found, although the presence of Ala(245) within the ISDR was associated with nonresponse to treatment in genotype 1a-infected patients (P<0.01). There were more mutations in the 26 amino acids downstream of the ISDR required for PKR binding in pretreatment isolates from responders versus nonresponders in both HCV-1a- and HCV-1b-infected patients (P<0.05). In HCV-1a patients, more amino acid changes were observed in isolates from IFN-sensitive patients (P<0.001), and the mutations appeared to be concentrated in two variable regions in the C terminus of NS5A, that corresponded to the previously described V3 region and a new variable region, 310 to 330. Selection of pretreatment minor V3 quasispecies was observed within the first 2 to 6 weeks of therapy in responders but not nonresponders, whereas the ISDR and PKR binding domains did not change in either patient response group. These data suggest that host-mediated selective pressures act primarily on the C terminus of NS5A and that NS5A can perturb or evade the IFN-induced antiviral response using sequences outside of the putative ISDR. Mechanistic studies are needed to address the role of the C terminus of NS5A in HCV replication and antiviral resistance.

Resistance of the hepatitis C virus (HCV) to interferon-alpha (IFN-alpha) therapy in patients with hepatitis C may be genetically controlled by an IFN sensitivity-determining region (ISDR) within the non-structural 5A (NS5A) gene. To assess whether HCV 1b strains carrying a 'resistant' type of ISDR are selected during unsuccessful IFN therapy, we analysed the evolution of the NS5A quasispecies, as detected by the clonal frequency analysis technique, and of the ISDR sequence by nucleotide sequence determination, in 11 patients showing no virological response during two consecutive cycles of IFN-alpha therapy. IFN-resistant patients had a homogeneous ISDR quasispecies with sequences identical to those described as 'resistant-' or 'intermediate-' type ISDR. After retreatment with IFN, further selection towards a homogeneous viral population was observed and 10 out of 11 patients had only one variant of HCV with no or just one single amino acid mutation within the ISDR sequence. Treatment and retreatment with IFN was associated in our non-responder patients with evolution of the ISDR quasispecies towards a rather homogeneous viral population carrying a conserved or minimally mutated ISDR motif, supporting the idea that this motif may be relevant for IFN resistance in HCV 1b-infected individuals.

Alpha interferon (IFN-alpha) treatment is effective on a long-term basis in only 15 to 25% of patients with chronic hepatitis C. The results of recent trials indicate that response rates can be significantly increased when IFN-alpha is given in combination with ribavirin. However, a large number of patients do not respond even to combination therapy. Nonresponsiveness to IFN is characterized by evolution of the hepatitis C virus (HCV) quasispecies. Little is known about the changes occurring within the HCV genomes when nonresponder patients are retreated with IFN or with IFN plus ribavirin. In the present study we have examined the genetic divergence of HCV quasispecies during unsuccessful retreatment with IFN or IFN plus ribavirin. Fifteen nonresponder patients with HCV-1 (4 patients with HCV-1a and 11 patients with HCV-1b) infection were studied while being retreated for 2 months (phase 1) with IFN-alpha (6 MU given three times a week), followed by IFN plus ribavirin or IFN alone for an additional 6 months (phase 2). HCV quasispecies diversification in the E2 hypervariable region-1 (HVR1) and in the putative NS5A IFN sensitivity determining region (ISDR) were analyzed for phase 1 and phase 2 by using the heteroduplex tracking assay and clonal frequency analysis techniques. A major finding of this study was the relatively rapid evolution of the HCV quasispecies observed in both treatment groups during the early phase 1 compared to the late phase 2 of treatment. The rate of quasispecies diversification in HVR1 was significantly higher during phase 1 versus phase 2 both in patients who received IFN plus ribavirin (P = 0.017) and in patients who received IFN alone (P = 0. 05). A trend toward higher rates of quasispecies evolution in the ISDR was also observed during phase 1 in both groups, although the results did not reach statistical significance. However, the NS5A quasispecies appeared to be rather homogeneous and stable in most nonresponder patients, suggesting the presence of a single well-fit major variant, resistant to antiviral treatment, in agreement with published data which have identified an IFN sensitivity determinant region within the NS5A. During the entire 8 months of retreatment, there was no difference in the rate of fixation of mutation between patients who received combination therapy and patients who were treated with IFN alone, suggesting that ribavirin had no major effects on the evolution of the HCV quasispecies after the initial 2 months of IFN therapy.

To investigate the role of hepatitis C virus (HCV) quasispecies mutation in the pathogenesis of HCV infection, we analyzed changes in the genetic diversity of HCV genomes in 22 patients before and after liver transplantation by using heteroduplex mobility assay (HMA) technology. All patients were infected with HCV genotype 1 and developed high-titer posttransplant viremia. Each patient was classified according to the severity of posttransplant hepatitis, as assessed by standard biochemical and histological criteria. HCV quasispecies were characterized by HMA analysis of eight separate subgenomic regions of HCV, which collectively comprise 44% of the entire genome. The glycoprotein genes E1 and E2, as well as the nonstructural protein genes NS2 and NS3, had the greatest genetic divergence after liver transplantation (the change in the heteroduplex mobility ratio [HMR] ranged from 2.5 to 7.0%). In contrast, genes encoding the core, NS4, and NS5b proteins had the least amount of genetic divergence after liver transplantation (range, 0.3 to 1.2%). The E1/E2 region showed the greatest change in genetic diversity after liver transplantation, and the change in HMRs was 2.5- to 3.3-fold greater in patients with asymptomatic or moderate disease than in those with severe disease. The E1-5' region of HCV quasispecies isolated from patients in the asymptomatic group had a significantly greater degree of diversification after liver transplantation than the same regions of HCV quasispecies isolated from patients in the severe disease group (P = 0.05). While changes in the genetic diversity of some nonstructural genes were also greater in asymptomatic patients or in patients with mild disease than in patients with severe disease, the results were not significant. Data from this cohort demonstrate that greater rates of HCV quasispecies diversification are associated with mild or moderate liver disease activity in this immunosuppressed population.

To study hepatitis C virus (HCV) genetic mutation during interferon (IFN) therapy, the temporal changes in HCV quasispecies heterogeneity were compared before and after treatment for nine patients infected with HCV genotype 1, including four nonresponders, four responders who relapsed after therapy, and one responder who experienced a breakthrough of viremia during therapy. Nine untreated patients with an average time between specimens of 8.4 years served as controls. Sequences from the second envelope glycoprotein gene hypervariable region 1 (HVR1) and the putative IFN sensitivity-determining region (ISDR) of the nonstructural NS5A gene were analyzed by heteroduplex mobility assays and nucleotide sequencing. A strong positive correlation was found between the percent change in a heteroduplex mobility ratio (HMR) and percent change in nucleotide sequence (r = 0.941, P < 0.001). The rate of fixation of mutations in the HVR1 was significantly higher for IFN-treated patients than for controls (6.97 versus 1.31% change in HMR/year; P = 0.02). Similarly, a higher rate of fixation of mutations was observed in the ISDR for IFN-treated patients than for untreated controls, although the result was not significant (1.45 versus 0.15 amino acid changes/year; P = 0.12). On an individual patient basis, IFN therapy was associated with measurable HVR1 and ISDR mutation in nine of nine (100%) and two of nine (22.2%) patients, respectively. Evolution to IFN-resistant ISDR sequences was observed in only one of nine IFN-treated patients. These data suggest that IFN therapy frequently exerts pressure on the HCV envelope region, while pressure on the ISDR was evident in only a subset of patients. Thus, the selection pressures evoked on HCV genotype 1 quasispecies during IFN therapy appear to differ among different patients.

Previous studies from Japan have described an association between a conserved sequence within the hepatitis C virus (HCV) genome and resistance to interferon (IFN) therapy for patients infected with HCV genotype 1b [Enomoto et al. (1995): Journal of Clinical Investigation 96: 224-230; Enomoto et al. (1996): New England Journal of Medicine 334:77-81]. The present study examines amino acid sequences surrounding the putative Interferon Sensitivity Determining Region (ISDR) of the NS5A gene of HCV in 21 North American patients with genotype 1a or 1b infection receiving recombinant IFN therapy. The ISDR consensus or intermediate pattern was observed in 13 of 14 NS5A clones from North American patients infected with genotype 1b. However, we found no evidence of the consensus ISDR sequence in any NS5A clones isolated from 15 patients with genotype 1a infection. In select cases, gel shift analysis showed no significant changes in the clonal frequency of the putative ISDR domain of HCV genotype 1a or 1b infected patients who were either nonresponsive to IFN therapy, or relapsed following withdrawal of IFN therapy. These results suggest that a conserved ISDR domain is neither associated with, nor responsible for, IFN resistance in North American patients infected with HCV genotype 1a, and demonstrate a need for further investigation into the reported association between ISDR consensus sequences and IFN resistance in genotype 1b clones.

Historically, the genus pestivirus was believed to contain three species of viruses; bovine viral diarrhea virus (BVDV), border disease virus (BDV) and classical swine fever virus (CSFV). However, based on limited sequence analysis of a small number of pestiviral isolates from domestic livestock, evidence has recently emerged indicating that at least four distinct genotypes exist. In an attempt to gain a better understanding of the degree of viral variation among ruminant pestiviruses, the entire structural gene coding region of an ovine pestivirus. BD31, genome encompassing 3358 nucleotides was cloned and sequenced. Sequence analysis revealed that BD31 shares less than 71% nucleotide similarity with other pestiviruses, suggesting, that BD31 is distinct from BVDV, CSFV as well as other ovine and bovine pestiviruses currently referred to as BVDV type II. Based on this data, BD31 is the first North American pestivirus isolate that falls under the category true BDV. Results from the analysis of the nucleotide sequence of the E0-E1 coding region of six additional ruminant pestiviruses identified the existence of three distinct virus genotypes in North America. Thus, among ruminent pestiviruses, bovine isolates can be grouped into two genotypes, namely types 1 and 4, whereas ovine isolates fall into genotypes 1, 3 and 4.

Viruses that comprise the Pestivirus genus cause significant losses to the livestock industry. Based on sequence analysis, currently 4 distinct genotypes are identified of which 3 infect cattle and sheep. Distinguishing between bovine and ovine isolates by serological tests has often been difficult because of a high degree of cross reactivity. In this study, a nested polymerase chain reaction (PCR) assay was developed to identify and distinguish between bovine viral diarrhea virus (BVDV) type I, BVDV type II, as well as border disease virus (BDV) genotypes. Consensus oligonucleotide primers were designed to amplify a 826-bp product from any of the 3 pestivirus types in a reverse transcription-PCR (RT-PCR). This product was subjected to a second round of nested PCR with type-specific primers which yielded DNA products of unique size characteristic for each pestivirus genotype. Using this assay, we were able to rapidly characterize several viral isolates and determine that all 3 genotypes can be found among ovine isolates.

Border disease virus (BDV) of sheep, an important ovine pathogen, is serologically related to the two other well characterized members of the Pestivirus genus of the Flaviviridae family, namely bovine viral diarrhea virus (BVDV) and hog cholera virus (HoCV). To determine its genetic relationship to BVDV and HoCV, the genome of BDV strain, BD-78 encompassing the 5' untranslated region (UTR) and structural gene coding region was molecularly cloned and the nucleotide sequence determined. The sequenced region of 3,567 nucleotides contained one open reading frame encoding 1063 amino acids. The nucleotide and amino acid sequences of BD-78 were compared with those of two BVDV strains NADL and SD-1, and the Alfort and Brescia strains of HoCV. The overall nucleotide sequence homologies of the region sequenced of BD-78 are 68.3% with BVDV-NADL, 67.8% with BVDV-SD-1, 69.0% with HoCV-Brescia, and 65.8% with HoCV-Alfort. The overall amino acid sequence homologies of BD-78 are 76.1% with NADL, 76.5% with SD-1, 74.2% with Brescia, and 72.9% with Alfort. The most conserved nucleotide and amino acid sequences between BD-78 and the other pestivirueses are in the 5' UTR and the capsid protein coding region (p14), where as the most divergent sequences are in the E2 coding region. These findings suggest that BDV is a unique virus in the Pestivirus genus.

Although nonverbal cues of dominance and the emotional responses they elicit have been well known since antiquity, the facial displays associated with successful political leadership have a direct political impact on the electorate in the television age. The effects of these stimuli can be studied experimentally from the perspective of human ethology. Recent findings indicate that expressive displays like those of nonhuman primates have similar effects when exhibited by human leaders in France and the United States but that cultural differences in expected behavior may significantly modify their effects., (Copyright © 1989. Published by Elsevier B.V.)