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Your search keyword '"Sittinger, Michael"' showing total 303 results
Start Over Author "Sittinger, Michael" Publication Type Academic Journals
303 results on '"Sittinger, Michael"'

3. 3D Inkjet Printing of Biomaterials with Solvent‐Free, Thiol‐Yne‐Based Photocurable Inks.

Author

Kainz, Michael, Haudum, Stephan, Guillén, Elena, Brüggemann, Oliver, Höller, Rita, Frommwald, Heike, Dehne, Tilo, Sittinger, Michael, Tupe, Disha, Major, Zoltan, Stubauer, Gerald, Griesser, Thomas, and Teasdale, Ian

Subjects
PHOTOCHEMICAL curing, TISSUE scaffolds, THREE-dimensional printing, BIOMATERIALS, TISSUE engineering, CYTOTOXINS, INK
Abstract

3D inkjet printing is a fast, reliable, and non‐contact bottom–up approach to printing small and large models and is one of the fastest additive manufacturing technologies available. These attributes position inkjet printing as a promising tool for the additive manufacturing of biomaterials, for example, tissue engineering scaffolds. However, due to the stringent technical rheological requirements of current inkjet technologies, there is a lack of photopolymer resins suitable for the inkjet printing of biomaterials. Hence, a novel ink engineered for 3D piezoelectric inkjet printing of biomaterials is designed and developed. The novel resin leverages a biodegradable amino acid phosphorodiamidate matrix copolymerized with a dialkyne ether to modulate the viscosity. Copolymerization with commercially available thiols facilitates the photochemical thiol‐yne curing reaction. The ink exhibits optimal viscosity, eliminating the need for solvents, as well as reliable jetting and sufficiently swift curing kinetics. Furthermore, the formulation is successfully demonstrated in an industrial inkjet printhead. Notably, the resulting materials have low cytotoxicity and, hence, have significant promise in advancing the applications of 3D inkjet printing of biological scaffolds. [ABSTRACT FROM AUTHOR]

Published
2024
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15. Blends of gelatin and hyaluronic acid stratified by stereolithographic bioprinting approximate cartilaginous matrix gradients.

Author

Shopperly, Lennard K., Spinnen, Jacob, Krüger, Jan‐Philipp, Endres, Michaela, Sittinger, Michael, Lam, Tobias, Kloke, Lutz, and Dehne, Tilo

Subjects
BIOPRINTING, HYALURONIC acid, GELATIN, EXTRACELLULAR matrix proteins, ARTICULAR cartilage
Abstract

Stereolithographic bioprinting holds great promise in the quest for creating artificial, biomimetic cartilage‐like tissue. To introduce a more biomimetic approach, we examined blending and stratifying methacrylated hyaluronic acid (HAMA) and methacrylated gelatin (GelMA) bioinks to mimic the zonal structure of articular cartilage. Bioinks were suspended with porcine chondrocytes before being printed in a digital light processing approach. Homogenous constructs made from hybrid bioinks of varying polymer ratios as well as stratified constructs combining different bioink blends were cultivated over 14 days and analyzed by histochemical staining for proteoglycans/collagen type II, cartilage marker expression analysis, and for cellular viability. The stiffness of blended bioinks increased gradually with HAMA content, from 2.41 ± 0.58 kPa (5% GelMA, 0% HAMA) to 8.84 ± 0.11 kPa (0% GelMA, 2% HAMA). Cell‐laden constructs maintained vital chondrocytes and supported the formation of proteoglycans and collagen type II. Higher concentrations of GelMA resulted in increased formation of cartilaginous matrix proteins and a more premature phenotype. However, decreased matrix production in central areas of constructs was observed in higher GelMA content constructs. Biomimetically stratified constructs retained their gradient‐like structure even after ECM formation, and exclusively exhibited a significant increase in COL2A1 gene expression (+178%). Concluding, we showed the feasibility of blending and stratifying photopolymerizable, natural biopolymers by SLA bioprinting to modulate chondrocyte attributes and to create zonally segmented ECM structures, contributing to improved modeling of cartilaginous tissue for regenerative therapies or in vitro models. [ABSTRACT FROM AUTHOR]

Published
2022
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22. Gene expression profiling of rheumatoid arthritis synovial cells treated with antirheumatic drugs

Author

Haupl, Thomas, Yahyawi, Mehdi, Lubke, Carsten, Ringe, Jochen, Rohrlach, Thorsten, Burmester, Gerd R., Sittinger, Michael, and Kaps, Christian

Subjects
Gene expression -- Research -- Genetic aspects, DNA microarrays -- Research -- Genetic aspects, Synovitis -- Research -- Genetic aspects, Drugs -- Product/Service Evaluations, Rheumatoid arthritis -- Genetic aspects -- Research, Biological sciences, Genetic aspects, Research
Abstract

Nonbiological therapeutics are frequently used for the treatment of patients with rheumatoid arthritis (RA). Because the mechanisms of action of these therapeutics are unclear, the authors aimed to elucidate the [...]

Published
2007

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