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Iodoacetic acid (IAA) is an emerging and the most genotoxic iodinated disinfection byproduct to date. IAA can disrupt the thyroid endocrine function in vivo and in vitro, but the underlying mechanisms remain unclear. In this work, transcriptome sequencing was used to investigate the effect of IAA on the cellular pathways of human thyroid follicular epithelial cell line Nthy-ori 3–1 and determine the mechanism of IAA on the synthesis and secretion of thyroid hormone (TH) in Nthy-ori 3–1 cells. Results of transcriptome sequencing indicated that IAA affected the TH synthesis pathway in Nthy-ori 3–1 cells. IAA reduced the mRNA expression of thyroid stimulating hormone receptor, sodium iodide symporter, thyroid peroxidase, thyroglobulin, paired box 8 and thyroid transcription factor-2, inhibited the cAMP/PKA pathway and Na+-K+-ATPase, and decreased the iodine intake. The results were confirmed by our previous findings in vivo. Additionally, IAA downregulated glutathione and the mRNA expression of glutathione peroxidase 1, leading to increased reactive oxygen species production. This study is the first to elucidate the mechanisms of IAA on TH synthesis in vitro. The mechanisms are associated with down-regulating the expression of genes related to TH synthesis, inhibiting iodine uptake, and inducing oxidative stress. These findings may improve future health risk assessment of IAA on thyroid in human.

The ubiquitous presence of biofilms in premise plumbing and stagnation, which commonly occurs in premise plumbing, can exacerbate the decay of chlorine residual in drinking water. Using biofilms grown in a simulated premise plumbing setup fed directly with freshly treated water at two full-scale water treatment plants, we previously determined the mass transfer coefficients for chlorine decay in premise plumbing. These coefficients coupled with inactivation kinetics of L. pneumophila released from biofilms reported previously were integrated into a Monte Carlo framework to estimate the infection risk of biofilm-derived L. pneumophila from 1 to 48 h of stagnation. The annual infection risk was significantly higher when water stayed stagnant for up to 48 h in pipes covered internally with biofilms, compared to clean pipes without biofilms. The decay of residual chlorine due to biofilms during 48-hour stagnation led to up to 6 times increase in the annual infection risk compared to the case where biofilms was absent. Global sensitivity analysis revealed that the rate of L. pneumophila detachment from biofilms and the decay of chlorine residual during stagnation are the two most important factors influencing the infection risks. Stagnation caused by water use patterns and water-saving devices in the premise plumbing can lead to increased infection risk by biofilm-derived L. pneumophila. Overall, this study’s findings suggested that biofilms could induce chlorine decay and consequently increase L. pneumophila infection risk. Thus, reducing stagnation, maintaining residual chlorine, and suppressing biofilm growth could contribute to better management of L. pneumophila infection risk. Keywords: Residual chlorine, Stagnation, Biofilms, Legionella pneumophila

As an important transportation facility, airports substantially affect the economic lives of people. However, the full extraction of airports located in a vast area is concerning. The size of an airport in a previous wide area detection framework is relatively large and has a strong saliency in remote sensing images, whereas the contradiction between a complex geographical background and a small airport size has yet to be resolved. In this study, we propose a set of automatic detection frameworks to realize efficient detection for various airports in nine Indian states/union territories under the condition that only runway samples are labeled. Preliminary extraction of runway features is performed with a high F1 and recall rate, and teacher nodes judge and guide the results. Next, the output is connected to classification and segmentation for outlier elimination and pixel extraction to locate the runways. For the study area, the proposed framework airport retention rate (RR) is 92.7%, with the false alarm reduction rate (FARR) reduced by a maximum of 95.3%. A total of 192 airports are discovered, and the effective airport growth rate (GR) is 47.4%. Compared to previous work, RR, GR, FARR, and run efficiency increased by 2.2%, 16.0%, 4.5%, and 432.5%, respectively, with more small- and medium-sized airports detected. Furthermore, the framework is tested in Japan, and 155 airports are detected. Thus, the proposed framework effectively improves detection capability for small- and medium-sized airports in large-scale areas and updates the airport database.

We investigated chronic mammalian cell cytotoxicity of wastewaters from four sources and their optical spectroscopic properties with or without chlorination or ozonation. Samples from effluents of activated sludge, nitrification tower, facultative waste stabilization pond, and maturation waste stabilization pond were either chlorinated or ozonated. The wastewater samples were analyzed for fluorescence excitation emission matrix, specific fluorescence index (SFI), and specific UV absorbance at 254 nm (SUVA). Before and after disinfection the wastewater samples were quantitatively analyzed for in vitro mammalian cell cytotoxicity. We found that the organic extracts from the ozonated samples induced lower cytotoxicity responses than those from the chlorinated or the nondisinfected samples. To develop correlations between SFI, SUVA, and cytotoxicity, we analyzed 21 independent samples. Significant linear correlations found among these samples suggest that under the tested conditions, cytotoxicity was preferentially influenced by the fluorescence and SUVA of their composite organic agents. These two spectroscopic parameters may be used as indicators for the potential cytotoxicity of nondisinfected, ozonated, or chlorinated municipal wastewaters. [ABSTRACT FROM AUTHOR]

Road traffic is occasionally blocked by landslide geological disasters in remote mountainous areas, causing obstruction to economic society and national defense construction. It is vital to conduct landslide geological disaster risk assessment and vulnerability research on the road network. Based on landslide geological disaster risk on the road network, this study analyzed the potential effects of the main environmental elements. Due to the lack of previous research works, this study proposed an effective, rational, and understandable multicriteria heuristic analytical hierarchy process model, fuzzy comprehensive evaluation, and frequency ratio-interactive fuzzy stack analysis for vulnerability assessment of road networks in large and complex networks. Based on the comprehensive use of geographic information technology, the road network vulnerability of Tibet in China was evaluated by introducing slope, topographic relief, normalized difference vegetation index (NDVI), annual mean precipitation, distance from river drainage, glaciers and snow, habitation, seismic center and geological fault zone, and soil erosion intensity. According to the findings of the study, the three-stage framework proposed in this study can provide correct inferences and explanations for the potential phenomena of landslide geological disasters; the geological disaster risk are unevenly distributed in the study area; the distribution of the road network vulnerability in China’s Tibet significantly differs among different cities; the high-vulnerability section presents significant regional characteristics, which overlap with the area with a high risk of landslide geological disasters, and its distribution is mostly located in traffic arteries, link aggregations, and relatively frequent human activity.

Water reuse is receiving unprecedented attention as many areas around the globe attempt to better-manage their fresh water resources. Wastewaters in coastal regions may contain elevated levels of bromide (Br-) and iodide (I-) from seawater intrusion or high mineral content in the source waters. Disinfection of such wastewater is essential to prevent the spread of pathogens; however, little is known about the toxicity of the treated wastewater. In this study, we evaluated the genotoxicity to Chinese hamster ovary (CHO) cells induced by municipal secondary wastewater effluent amended with elevated Br- and I- after disinfection by chlorine, chloramines, or ozone. We calibrated and applied an N-acetylcysteine (NAC) thiol reactivity assay as a surrogate for thiol reactivity with biological proteins (glutathione) of wastewater samples. Chlorination of wastewaters produced CHO cell genotoxicity comparable to chloramination, 3.9 times more genotoxic than the nondisinfected controls. Ozonated wastewater was at least 3 times less genotoxic than the samples treated with chlorine-based disinfectants and was not significantly different compared with the nondisinfected controls. Positive and significant correlations were observed among genotoxicity, cytotoxicity, and NAC thiol reactivity for all disinfected samples. These results indicate that the ozonation of wastewater with high Br- and I- levels may yield organics with lower genotoxicity to CHO cells than chlorine-based disinfection. NAC thiol reactivity, although excluding the possible effect of bromate from ozonation in this work, could be used as a rapid in chemico screen for potential genotoxicity and cytotoxicity in mammalian cells exposed to disinfected wastewaters. [ABSTRACT FROM AUTHOR]

Reusing wastewater in agriculture is becoming increasingly common, which necessitates disinfection to ensure reuse safety. However, disinfectants can react with wastewater constituents to form disinfection byproducts (DBPs), many of which are toxic and restrict the goal of safe reuse. Our objective was to benchmark the induction of mammalian cell cytotoxicity after ozonation against chlorination for three types of real wastewaters: municipal secondary effluent and two sources of minimally treated swine farm wastewaters. A new method to evaluate samples of suspected high cytotoxicity was devised. For the secondary effluent, ozonation reduced the cytotoxicity by as much as 10 times; chlorination lowered the cytotoxicity only when followed by dechlorination. The swine farm wastewaters were up to 2000 times more cytotoxic than the secondary effluent, and the highest reduction in cytotoxicity was 17 times as achieved by ozonation. These results indicate that secondary effluent is preferred over swine wastewaters for agricultural reuse regardless of the tested disinfectants. Ozonation consistently reduced the cytotoxicity of both the full strength and the organic extracts of all tested wastewaters more than chlorination. The only significant correlation was observed in the secondary wastewater between total haloacetonitriles and cytotoxicity. While the association of reduced toxicity with the modification or reduction of specific compound(s) is unclear, regulated DBPs may not be the primary forcing agents. [ABSTRACT FROM AUTHOR]

Background: Bacteroides ovatus, a member of the genus Bacteroides, is considered for use in molecular-based methods as a general fecal indicator. However, knowledge on its fate and persistence after a fecal contamination event remains limited. In this study, the persistence of B. ovatus was evaluated under simulated sunlight exposure and in conditions similar to freshwater and seawater. By combining propidium monoazide (PMA) treatment and quantitative polymerase chain reaction (qPCR) detection, the decay rates of B. ovatus were determined in the presence and absence of exogenous photosensitizers and in salinity up to 39.5 parts per thousand at 27°C. Results: UVB was found to be important for B. ovatus decay, averaging a 4 log10 of decay over 6 h of exposure without the presence of extracellular photosensitizers. The addition of NaNO2, an exogenous sensitizer producing hydroxyl radicals, did not significantly change the decay rate of B. ovatus in both low and high salinity water, while the exogenous sensitizer algae organic matter (AOM) slowed down the decay of B. ovatus in low salinity water. At seawater salinity, the decay rate of B. ovatus was slower than that in low salinity water, except when both NaNO2 and AOM were present. Conclusion: The results of laboratory experiments suggest that if B. ovatus is released into either freshwater or seawater environment in the evening, 50% of it may be intact by the next morning; if it is released at noon, only 50% may be intact after a mere 5 min of full spectrum irradiation on a clear day. This study provides a mechanistic understanding to some of the important environmental relevant factors that influenced the inactivation kinetics of B. ovatus in the presence of sunlight irradiation, and would facilitate the use of B. ovatus to indicate the occurrence of fecal contamination. [ABSTRACT FROM AUTHOR]

A greater than four log10 reduction in the concentration of Legionella pneumophila in municipal wastewater has been achieved in 1 min with ozone produced by a microchannel plasma reactor. Requiring less than 22 W of electrical power, and ambient air as the feedstock gas, the microplasma ozone generator is robust and a promising alternative to conventional corona and dielectric barrier discharge (DBD) technologies. Contrary to previous studies, the Ct model for pathogen deactivation (i.e. rate proportional to the product of the available disinfectant concentration and the exposure duration) is found to be valid for L. pneumophila. Accordingly, wastewater-specific Ct equations have been developed to predict the deactivation of L. pneumophila in the secondary wastewater environment. Inactivation of this pathogen was found to be dependent on temperature only in the absence of wastewater organic matter (WOM). In the presence of WOM, pathogen deactivation is controlled by the disinfection contact time, initial ozone concentration (varied between 15 and 281 µg l−1), and initial WOM loading. The data reported here will assist in the implementation of plasma ozone generators for L. pneumophila deactivation in cooling towers, point-of-use systems, and wastewater reclamation facilities. [ABSTRACT FROM AUTHOR]